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Citations for Thermo Fisher :
4751 - 4800 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The library is quantified through qPCR (Invitrogen™ Collibri™ Library Quantification Kit; QuantStudio™ 5 Real Time PCR Systems, APPLIED BIOSYSTEMS).
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bioRxiv - Physiology 2024Quote: ... One-step reverse transcription and real-time PCR was performed with a Quantstudio 5 using Power SYBR Green RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) following the manufacturer protocol ...
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bioRxiv - Physiology 2024Quote: Quantitative real-time PCR was performed by extracting RNA from quadricep muscle lysates using PureLink mRNA kits (Thermo Scientific cat # 12183-018A) and synthesizing cDNA using a high capacity first strand cDNA synthesis kit (Thermo Scientific cat # 4368813) ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was conducted using the Thermoscript RT-PCR system (Invitrogen, Carlsbad, CA) and Ex Taq (Takara ...
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bioRxiv - Developmental Biology 2022Quote: ... RT-PCR was run on a 7900HT Fast RT-PCR instrument (Applied Biosystems) with Taqman probes for Fev (assay ID ...
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bioRxiv - Immunology 2023Quote: ... Gene expression was analyzed by quantitative reverse transcription PCR (qRT-PCR) analysis on a QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) and normalized to endogenous control gene mGUS ...
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bioRxiv - Molecular Biology 2023Quote: ... The quantitative real-time PCR (qRT-PCR) experiments were performed utilizing the POWRUP SYBR MASTER MIX via a StepOnePlus Real-time PCR instrument (Applied Biosystems, Texas, USA).
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bioRxiv - Genomics 2024Quote: ... Routine mycoplasma testing (PCR mycoplasma detection kit, Thermo Scientific, Waltham, MA) was performed as infrequently as every 6 months and as frequently as monthly ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently measured by real-time PCR with the Taqman universal PCR master mix and specific Taqman probes (Applied Biosystems). Sequences of primers are shown in Supplementary Table 2.
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR (qPCR) was performed using Fast Sybr Green chemistry on the 7900HT Fast Real-Time PCR System (Applied Biosystems) and gene-specific primers (Supplemental Table 1) ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative reverse transcriptase PCR (qPCR) was carried out by QuantStudio® 12 K Flex Real-Time PCR System (Life Technologies) using TaqMan Gene Expression Assay (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative real-time PCR was run on a ViiA 7 or QuantStudio 6 Flex PCR thermal cycler (Thermo Fisher Scientific) using SensiMix™ SYBR® No-ROX Kit (Bioline ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... 1μl of cDNA was used to set up real time qRT-PCR in a 20µl reaction system with 2X SYBR Green PCR master mix (Life Technologies). Experimental setup and execution were conducted using an ABI 7500 Fast Real-Time PCR system ...
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bioRxiv - Molecular Biology 2021Quote: ... were used for each luciferase and two reference genes were also quantified with qRT-PCR using an ABI QuantStudio 5 Real-Time PCR System (ThermoFisher). Three technical replicate reactions were performed for each unique primer set for each sample.
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bioRxiv - Microbiology 2019Quote: ... enabled us to perform quantitative PCR on an Applied Biosystems 7500 Fast Real-Time PCR System (Applied Biosystems, CA, USA) using the manufacturer’s instructions for a standard cycling protocol ...
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bioRxiv - Physiology 2019Quote: ... and 300nM of forward and reverse primer in a 25µl Power SYBR Green PCR Master Mix reaction in the StepOne-Plus Real-Time PCR System (Applied Biosystems). Gene expression was normalized to mouse RPS29 gene expression ...
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bioRxiv - Genetics 2019Quote: ... qPCRs were performed with a 7500 real-time PCR amplification system using SYBR Green PCR master mix (Applied Biosystems, UK). The relative levels of transcripts were calculated using the ΔΔCT method within the ABI 7500 System Software (V2.0.4) ...
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bioRxiv - Biochemistry 2019Quote: ... following the manufacturer’s instructions and qRT-PCR was performed with a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using PerfeCTa SYBR Green SuperMix ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time quantitative PCRs were performed in the presence of Power SYBR green PCR Master Mix (Applied Biosystems, Guangzhou, China). Amplification was monitored in real-time with the MiniOpticon™ Real-Time PCR System (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... Gene sequences were obtained from Ensembl v92 (www.ensembl.org) and PCR primers (Supplementary Table 16) were designed using Primer Express Software for Real-Time PCR 3.0 (Applied Biosystems). Primer efficiency and specificity were evaluated on genomic DNA in each species ...
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bioRxiv - Microbiology 2020Quote: ... real-time quantitative PCR analysis with a gene specific primer pair using SYBR green PCR mix (Applied Biosystems, cat: 4385612) was performed and data was analysed on the Applied Biosystems ViiATM 7 Real-Time PCR System ...
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bioRxiv - Neuroscience 2020Quote: ... mRNA expression levels were assessed by quantitative real-time PCR using SYBR Green dye-based PCR amplification (Thermo Fisher Scientific) and the QuantStudio 3 detection system (Applied biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... and SYBR Green PCR reagents according to the manufacturer’s instructions on a StepOnePlusTM Real Time PCR System (Thermo Fisher Scientific). The primers used are listed in Table S2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative PCR (qPCR) was performed using THUNDERBIRD SYBR qPCR Mix (TOYOBO) and Step One Real-Time PCR System (Applied Biosystems).
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bioRxiv - Cancer Biology 2020Quote: PCR amplification was performed in technical triplicates on the Applied Biosystems QuantStudio 3 Real-Time PCR System (Applied Biosystems-ThermoFisher). The PCR reaction contained SimpleChip Universal master mix (catalogue # 88989 ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplification was performed in technical triplicates on the Applied Biosystems QuantStudio 3 Real-Time PCR System (Applied Biosystems-ThermoFisher). TaqMan Universal PCR Master Mix (Applied Biosystems-ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: PCR amplification was performed in technical triplicates on the Applied Biosystems QuantStudio 3 Real-Time PCR System (Applied Biosystems-ThermoFisher). The PCR reaction contained SimpleChip Universal master mix (catalogue # 88989 ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplification was performed in technical triplicates on the Applied Biosystems QuantStudio 3 Real-Time PCR System (Applied Biosystems-ThermoFisher). TaqMan Universal PCR Master Mix (Applied Biosystems-ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... Transcript abundance was quantified on a QuantStudio 6 Real Time PCR system using SYBR® Green PCR Master Mix (ThermoFisher) and gene-specific primers (Supplementary Table S5) ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR reaction procedure was carried out on the ABI 7900HT Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA) with the AceQ qPCR SYBR Green Master Mix (Vazyme ...
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bioRxiv - Neuroscience 2022Quote: ... The real-time quantitative PCR (qRT-PCR) was performed with TaqMan Fast Advanced Master mix (ThermoFisher Scientific, catalog n°4444963) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: RT-qPCR was carried out on the Applied Biosystems 7500 Real Time PCR system using Power SYBRgreen PCR master mix (Invitrogen), 2 ng of cDNA per reaction and the following cycling conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... RealTime PCR was performed on a 7900HT Fast Real-Time PCR System with TaqMan® Array Block (Thermo Fisher Scientific), using universal cycling conditions (95 °C/10 min ...
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bioRxiv - Microbiology 2021Quote: ... The number of PCR cycles for sufficient DNA for downstream applications was empirically determined by quantitative PCR (ViiA 7 Real-Time PCR System, ThermoFisher) in the presence of 1X Evagreen (20X Evagreen ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative PCR was performed using PowerUP SYBR Green master mix on an ABI StepOnePlus real-time PCR instrument (Applied Biosystems). All PCR reactions were run in triplicates ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Real-time PCR was performed using the Power SYBR green PCR master mix in an ABI Prism 7900 (Applied Biosystems). The RNA probes used for the detection of endogenous tpm1 ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was performed using the qPCRBio SyGreen Mix Hi-ROX (PCR biosystems) according to the manufacturer’s instructions and run on the Real Time PCR QuantStudio3 (ThermoFisher scientific) using the following conditions – Initial denaturation step at 95□ °C for 2 minutes ...
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bioRxiv - Genomics 2019Quote: ... The qRT-PCR was conducted by an Applied Biosystems 7500 Real Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) and SYBR Premix Ex Taq™ II (TaKaRa ...
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bioRxiv - Microbiology 2019Quote: ... qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems) on an Applied Biosystems 7900HT real-time PCR machine in 96-well-plate format ...
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR measurements were obtained in a 7900HT Fast Real Time PCR System with ExpressionSuite Software v1.0 (Applied Biosystems). Taqman probes utilized in this study are listed in S-Table 9.
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bioRxiv - Plant Biology 2021Quote: ... qPCR was performed on a StepONE Plus Real-Time PCR system with Fast SYBR-Green PCR master mix (Applied Biosystems). FLC primer sequences were as previously described (Csorba et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... qRT-PCR reactions were performed using SYBR Green Master Mix in a 7500 Fast Real Time PCR cycler (Applied Biosystems). The TATA-box-binding protein domain gene was used as the internal control ...
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bioRxiv - Cancer Biology 2019Quote: ... Fast SYBR™ Green quantitative PCR was performed on a Step One™ Plus Real-Time PCR System (Applied Biosystems). Sequence of all RT-qPCR primers are shown in Supplementary Table 1.
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... mRNA expression levels were determined by real-time PCR using SYBR™ Green PCR Master Mix (Life Technologies, California, USA) on an ABI 7500 system ...
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bioRxiv - Microbiology 2019Quote: ... All quantitative PCR were performed in triplicate on an ABI 7500 Fast real-time PCR system (Applied Biosystems, Carlsbad, USA) using TransStart Tip Green qPCR SuperMix (Transgen ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems) on an Applied Biosystems 7900HT real-time PCR machine in 96-well-plate format ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR was performed using qPCRBIO SyGreen Blue Mix Lo-ROX (PCR biosystems) and the QuantStudio 5 Real Time PCR system (ThermoFisher). The data were analysed using QuantStudio Design and Analysis software (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... Quantitative PCR reactions were carried out using PowerUp SYBR-Green master mix using StepOne Real-Time PCR systems (Applied Biosystems). GAPDH mRNA levels were used for normalization ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was performed using SYBR Select Master Mix and QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) with the following parameter ...
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bioRxiv - Cancer Biology 2022Quote: ... This was followed by quantitative real-time PCR using Power SYBR Green PCR Master Mix [Thermo Fisher Scientific (Applied Biosystems), Waltham ...