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Citations for Thermo Fisher :
4801 - 4850 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... MHV-A59 was quantified by RT-qPCR by amplifying a fraction of its ORF5 gene for the structural protein M.15,16 The TaqMan™ Fast Virus 1-Step Master Mix Kit (Thermo Fisher Scientific Inc., 4444432) was used for RT-qPCR ...
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bioRxiv - Microbiology 2020Quote: ... and Mayaro virus (MAYV, TRVL 15537) were propagated in Aedes albopictus C6/36 cells grown in Minimum Essential Medium (MEM, Thermo Fisher Scientific, Waltham, MA). SARS-CoV-2 (SARS-CoV-2/CANADA/VIDO 01/2020 ...
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bioRxiv - Neuroscience 2021Quote: Images of whole brain slices for analysis of virus expression were obtained from a multichannel fluorescence microscope (EVOS FL Auto Imaging System, Thermo Fisher Scientific, Waltham, MA) with an automatic objective at 10X magnification ...
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bioRxiv - Microbiology 2021Quote: ... cDNA were synthesized from 100 pg of RNA and quantified using the TaqMan® Fast Virus 1-Step Master Mix (Thermofisher, Waltham, Massachusetts, USA) following manufacter’s instructions with appropriate oligonucleotides and Taqman probes (Table S1) ...
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bioRxiv - Immunology 2020Quote: Subgenomic RT-qPCR was performed on the QuantStudio™ 7 Flex Real-Time PCR System using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with forward primer ...
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bioRxiv - Immunology 2021Quote: The RBDwt protein was conjugated to Cucumber mosaic virus - tetanus toxoid (CuMVTT) using the linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, USA). CuMVTT contains a powerful T cell epitope of the Tetanus toxin (TT ...
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bioRxiv - Microbiology 2022Quote: ... The gradient was collected into 500 μL fractions and the virus containing fractions were detected using NanoDrop 1000 Spectrophotometer (Thermo Scientific, Waltham, Massachusetts, US). The virus containing sucrose fractions were diluted at least 1:5 in 2 mM MgCl2-PBS and the virus was pelleted by centrifugation (70Ti rotor ...
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bioRxiv - Microbiology 2023Quote: Cell culture supernatants or P1 virus cultures were mixed with a five-fold excess of TRIzol™ LS Reagent (Thermo Fisher Scientific, Waltham, MA). Viral RNAs were extracted by Direct-Zol™ RNA miniprep Plus Kit (ZYMO RESEARCH ...
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bioRxiv - Microbiology 2023Quote: ... Isolated RNA was subjected to one-step RTqPCR analysis as described using primers specific for the viral genome encoding for the RNA-dependent RNA polymerase (RdRp)23 and for E24 genes with TaqMan fast virus 1-step master mix (ThermoFisher Scientific, catalogue number 4444432) using AriaMx instrumentation (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... The infusion line was primed with a loading line solution that was identical to the virus suspension solution [Corning phosphate-buffered saline without Ca2+ or Mg2+ with 350 mM NaCl (Gibco, ThermoFisher Scientific, Waltham, MA) containing 0.001% F68 surfactant (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Isolated RNA was directly subjected to one-step RT-qPCR analysis using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, MA, USA) and Bio-Rad CFX instrumentation (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Ten micrograms of total RNA was reverse transcribed (RT) at 37 °C using random hexamer primers and Moloney murine leukemia virus retrotranscriptase (Invitrogen-Life Technologies, Buenos Aires, Argentina) in a 20 µL reaction mixture ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor® 488 goat anti-rabbit IgG (H+L) and Alexa Fluor® 555 goat anti-rabbit IgG (H+L) (Life Technologies, Carlsbad, California, USA).
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bioRxiv - Evolutionary Biology 2020Quote: DNA was extracted from pools of individuals (‘strain’ henceforth) derived from a single worm collected from a single experimentally infected fish using MagJET Genomic DNA kits (Thermo Scientific). DNA concentration was measured using Qubit High Sensitivity reagents ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant plasmid was then electroporated into competent strains S250ΔfimB:: or CES131CΔfimB:: and transformants were selected on lysogeny broth (LB) (Invitrogen, Carlsbad, California, USA) agar plates containing 100 mg/L of kanamycin ...
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bioRxiv - Genomics 2020Quote: DNA fragments containing telomere-proximal SH regions (∼5 kb) were amplified by PCR from genomic DNA of each 972SD4 strain using Phusion High-Fidelity DNA polymerase (Thermo Fisher) and the following primer set.
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bioRxiv - Microbiology 2019Quote: ... 50 μL of each strain inoculum was added to 50 μL of a two-fold serial dilution of rifampicin (Fisher Scientific) at a final concentration range 64-0.000975 μg ml-1 in Mueller Hinton broth (MHB) ...
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bioRxiv - Genetics 2019Quote: ... a male mouse (strain 129S2/SV) was sacrificed and the entire brain was harvested and grained in Trizol (Thermo Fisher Scientific). Total RNA was obtained according to the manufacture’s protocol ...
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bioRxiv - Immunology 2019Quote: ... First-strand cDNA for each strain was amplified using Thermo Fisher Phusion HF Buffer (Thermo Fisher, Cat. No. F530S; Waltham, MA) for 30 PCR cycles ...
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bioRxiv - Synthetic Biology 2020Quote: ... HEK239T cells (ATCC, strain number CRL-3216) used for part of this study were maintained in DMEM (Sigma-Aldrich or Gibco) supplemented with 10% FBS (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2019Quote: ... aeruginosa used for cloning were amplified from the genomic DNA of the PAO1 strain with Phusion High-Fidelity DNA polymerase (ThermoFisher Scientific). The primers used are listed in supplementary Table S3 of the Supporting Information ...
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bioRxiv - Microbiology 2020Quote: The spike protein gene from MERS-CoV strain Camel/Qatar/2/2014 (GenBank accession number KJ650098.1) was codon optimized for humans and synthesized by GeneArt (Thermo Fisher Scientific). The synthesized S gene was cloned into a transgene expression plasmid comprising a modified human cytomegalovirus immediate early promoter (CMV promoter ...
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bioRxiv - Microbiology 2021Quote: ... and the expression plasmid encoding the GP gene of an EBOV Makona strain (GenBank accession no. KJ660346) or BDBV Uganda strain (GenBank accession no. KR063673) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher). After 48-72 h ...
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bioRxiv - Systems Biology 2020Quote: ... The block 1 and 7 sequences were synthesized either by PCR amplification from the genomic DNA (isolated from sequenced fly strain, stock number 2057 in Bloomington Drosophila Stock Center) or by oligo synthesis from Life Technologies (for HindIII recognition sites mutated and ATGs mutated sequences) ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid reporter was transformed into appropriate strains and GFP fluorescence was measured over growth in a multi-mode plate reader (Varioskan-Thermo Scientific). For comparison across strains GFP fluorescence was normalized to optical density.
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bioRxiv - Microbiology 2021Quote: Total genomic DNA of the three studied strains (LEGE 06013, LEGE 11480 and LEGE 11467) was isolated using MOBIO Ultraclean DNA Isolation Kit (Life Technologies). To obtain the 16S rRNA gene and the 16S-23S Internal Transcribed Spacer (ITS ...
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bioRxiv - Biophysics 2020Quote: ... 10μg of linearized plasmid was transformed into Pichia pastoris X-33 strain by electroporation as per the manufacturers protocol (Thermo Fisher). Transformants were selected on Zeocin containing YPDS plates at a Zeocin concentration of 2mg/mL (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... using the ZIKV-F2 (5’-CAGCTGGCATCATGAAGAATC-3’) and ZIKV-R1 (5’-CACTTGTCCCATC TTCTTCTCC-3’) primers for African strain detection (ThermoFisher SCIENTIFIC) or the ZIKV-F1 (5’-CAGCTGGCATCATGAAGAACC-3’ ...
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bioRxiv - Bioengineering 2022Quote: The strains experienced by the PDMS membrane were measured using a microscope (Evos XL Core, Thermo Fisher Scientific, Waltham, MA, USA) by examining the displacement of ink dots marked on the PDMS membrane ...
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bioRxiv - Microbiology 2019Quote: ... respectively were PCR-amplified from genomic DNA of IPO323 or 06STD024 strains and the fragments cloned by BP cloning using Gateway™ BP Clonase II Enzyme Mix (Invitrogen) into pDONR-P4-P1R (upstream regions ...
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bioRxiv - Immunology 2020Quote: Tachyzoites of Toxoplasma gondii strains were passaged in ‘Toxo medium’ [4.5 g/liter D-glucose in DMEM with GlutaMAX (Gibco, cat#10566024), 1% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2020Quote: ... cDNAs from pUC57 entry vector were transformed by electroporation into Escherichia coli strain DH10B and transferred by a recombinant LR reaction of Gateway cloning (Clonase II enzyme mix Kit, Thermo Fisher) into pB7WGF2 (Karimi et al. ...
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bioRxiv - Bioengineering 2020Quote: ... bacterial suspensions for each strain were prepared by diluting the starter culture in minimum medium M63 supplemented with 20% glycerol (J910500G, Fisher Scientific) to 1:10 dilutions for E ...
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bioRxiv - Microbiology 2020Quote: ... Ten different Bacteroides strains in stationary phase (1mL) were subcultured into 9 mL of liquid sABB (Anaerobe Basal Broth, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... the strains were propagated at 30°C with constant shaking in sterile Nunc 96 deep-well storage blocks (Thermo Scientific, 260252), covered with an AeraSeal (Excel Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Fluorescent signals of eGFP production in wild type and mutant strains were measured by flow cytometry (Attune NxT Flow Cytometer, Thermo Fisher) (53).
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bioRxiv - Microbiology 2020Quote: Rabbit serum immunized with the DENV-2 NGC strain was coated onto Nunc Maxisorp ELISA plates (Thermo Fisher Scientific, Waltham, MA). Then ...
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bioRxiv - Genomics 2022Quote: Single colonies of strains were grown for two days without shaking at 30C in a 96-well deep-well plate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... strains were cultivated separately at 30°C on YPDF medium that contained 1% yeast extract (Oxoid, Thermo Fisher Scientific, Waltham, USA), 2% bactopeptone (BD ...
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bioRxiv - Microbiology 2022Quote: ... individual strains were cultivated at 30°C on YPDF agar that contained 1% yeast extract (Oxoid, Thermo Fisher Scientific, Waltham, USA), 2% bactopeptone (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... brucei single marker 427 strain bloodstream forms were grown in HMI-9 medium supplemented with 10% inactivated Fetal Bovine Serum (FBS, Life Technologies) 21 and 2 µg/mL of G418 (Biobasic ...
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bioRxiv - Microbiology 2022Quote: ... and the resulting solution was used to perform multiplex PCR using primer pairs specific for each endogenous plasmid of strain B31 [61] and the DreamTaq Green DNA Polymerase (Thermo Scientific). For genomic DNA extraction ...
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bioRxiv - Microbiology 2023Quote: Conidia suspensions were prepared for the different experiments by cultivating the fungal strains for six days at 25°C on potato dextrose agar (PDA, Difco, Fisher Scientific). Two mL of Tween 80 (0.045 % ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2/UT-NCGM02/Human/2020 (Wuhan strain) was propagated in VeroE6/TMPRSS2 cells in VP-SFM (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... Salmonella enterica serovar Typhimurium strain SL1344 and Listeria monocytogenes strain 10403s (both generous gifts from Dr. Subash Sad, University of Ottawa) were cultured in Lysogeny Broth (LB, Fisher Scientific). Bacteria were inoculated into LB from frozen glycerol stocks and grown at 37°C with shaking (200 rpm).
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bioRxiv - Microbiology 2024Quote: Conidium suspensions were prepared for the various experiments by cultivating the fungal strains for six days at 25°C on potato dextrose agar (PDA, Difco, Fisher Scientific). Two mL of Tween 80 (0.045 % ...
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bioRxiv - Microbiology 2024Quote: ... falciparum strains were cultured at 2% haematocrit in O+ erythrocytes and RPMI-1640 medium (Lonza, BE12-167F or Gibco, 31870-025) supplemented to give the following final concentrations ...
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bioRxiv - Molecular Biology 2024Quote: ... the pellet was resuspended with MOPS minimal medium and 190 μl of all the strains were placed in a 96 well black/clear bottom plate (Thermo Fisher) and the OD600 and fluorescence was measured ...
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bioRxiv - Microbiology 2024Quote: ... strains overexpressing lpg0275, lpg0969 and lpg2482 (sdbA, ravK, and sdbB respectively) from the high-copy vector pYES2 NT/A (Life Technologies, GAL 1 promoter ...
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bioRxiv - Immunology 2024Quote: ... major Friedlin (MHOM/SU/73/5-ASKH) strain (1×105 logarithmic promastigotes per mL) were cultured at 26 °C in M199 medium (Gibco, USA) (pH 7.4 ...