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Citations for Thermo Fisher :
4751 - 4800 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... RNA was isolated and cDNA generated from 10 young adult animals per strain and condition using the Power SYBR Green Cells-to-Ct kit (Invitrogen) according to the method described by Chauve et al (Chauve et al ...
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bioRxiv - Developmental Biology 2021Quote: ... were amplified from the genomic DNA of the RW strain and were cloned into pCR Blunt II-TOPO vector (Invitrogen). pDestTol2pA2-charon:EGFP (charon:EGFP for short ...
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bioRxiv - Cell Biology 2021Quote: Gene deletions were generated by PCR amplification of the locus-specific KanMX cassette from template genomic DNA obtained from the relevant strains of the gene disruption collection (Invitrogen) using primers annealing upstream and downstream of the genomic loci and subsequent transformation into the W303 background ...
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bioRxiv - Neuroscience 2021Quote: ... inactivated mouse embryonic fibroblasts (EmbryoMax® PMEF, strain CF-1) in hESC medium consisting of Knockout DMEM (#10829-018, Invitrogen) with 20% Knockout Serum Replacement (#10828-028 ...
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bioRxiv - Bioengineering 2021Quote: ... The wild-type and drug-resistant IAV strains were propagated in MDCK cells cultured in DMEM supplemented with 1% bovine serum albumin (Gibco) and 2 µg/mL l-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin at 34 °C for 48 h ...
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bioRxiv - Systems Biology 2020Quote: ... the different Sla1 engineered proteins were constructed using the BY4741 Sla1-GFP strain from the GFP collection (Thermo Fisher Scientific) (43 ...
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bioRxiv - Microbiology 2021Quote: Verticillium dahliae strain JR2 (CBS 143773; Faino et al., 2015) was cultured on potato dextrose agar (PDA) (Oxoid, Thermo Scientific) at 22°C in the dark ...
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bioRxiv - Microbiology 2021Quote: Well-differentiated camelid AEC cultures were infected with 4000 PFU of MERS-CoV (strain EMC/2012, propagated on Huh7 cells diluted in Hanks balanced salt solution (HBSS, Gibco). The cells were washed with 100 μl of HBSS prior to infection (18) ...
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bioRxiv - Microbiology 2021Quote: ... if sequences diverged between strains) were codon-optimised for expression in human cells and synthesised by GeneArt (Thermo Fisher Scientific), with an optimal 5’ Kozak sequence and fused to an N-terminal FLAG epitope ...
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bioRxiv - Microbiology 2020Quote: ... 100μg of whole cell lysate from each strain was run on NuPage 4-12% bis-tris protein gel (Thermo Scientific) at 120V for 3hours ...
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bioRxiv - Cell Biology 2021Quote: The laboratory strain of Leishmania donovani (Dd8) was routinely maintained in high glucose Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Life Technologies) supplemented with 10% of heat-inactivated foetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... simulans mh genomic region covering the coding region and ~3000 bp upstream was PCR amplified from the strain w501 using oligos 2099/2100 and cloned into pCR-Blunt II-TOPO (Invitrogen). The sequence of sim-mh was confirmed by Sanger sequencing (using oligos 788 ...
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bioRxiv - Synthetic Biology 2022Quote: Transformations for plasmid targeting experiments were performed in chemically competent PIR1 cells containing pTarget (original PIR1 strain obtained from Invitrogen), using 25 ng of pCAST or pHELIX and 25 ng of pDonor ...
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bioRxiv - Microbiology 2022Quote: ... parahaemolyticus strains were grown in LB-Miller at 37°C and diluted in DMEM (Dulbecco Modified Eagle’s Medium, Invitrogen #11995) to generate a multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2022Quote: ... All strains were washed with PBS and diluted in Dulbecco’s Modified Eagle Medium (DMEM) without phenol red (Gibco, Life technologies). Number of bacteria were quantified by absorbance measurement at OD600 with an estimation of 2.5×108 bacteria in 1 ml of 1.0 OD600 ...
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bioRxiv - Microbiology 2021Quote: ... velezensis strains were prepared and inoculated into the wells of 24-well polyvinylchloride (PVC) microtiter plates (Fisher Scientific, Shanghai, China) filled with MSgg medium as described previously (52) ...
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bioRxiv - Microbiology 2020Quote: ... dahliae strain JR2 (CBS 143773; Faino et al., 2015) was maintained on potato dextrose agar (PDA) (Oxoid, Thermo Scientific, CM0139) and grown at 22°C in the dark ...
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bioRxiv - Microbiology 2021Quote: Two-day biofilm formation of Pseudomonas strains was determined by crystal violet staining using a 96-well plate lid with pegs extending into each well (Nunc-TSP lid ...
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bioRxiv - Microbiology 2022Quote: ... All strains were washed with PBS and diluted in Dulbecco’s Modified Eagle Medium (DMEM) without phenol red (Gibco, Life technologies). Number of bacteria were quantified by absorbance measurement at OD600 with an estimation of 2.5×108 bacteria in 1 ml of 1.0 OD600 ...
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bioRxiv - Microbiology 2019Quote: ... rodA2 and rodA3 in the different strains was assessed using the TaqMan® probe-based gene expression assay (Applied Biosystems). Expression of gyrB was used as control ...
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bioRxiv - Microbiology 2019Quote: ... Standard curves for gfp and oriV were constructed using plasmid isolated from the ancestral strain using the PureLink Plasmid DNA Miniprep Kit (Invitrogen). Total DNA amounts were quantified using a Qubit Fluorometer (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... All HTM cell strains used in this study were validated with dexamethasone (DEX; Fisher Scientific, Waltham, MA, USA; 100 nM) induced myocilin (MYOC ...
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bioRxiv - Molecular Biology 2021Quote: ... For Protein A-tagged strains, immunoprecipitation was carried out with Rabbit IgG-conjugated (MP-Biomedicals, SKU 085594) Dynabeads (Invitrogen, 14301) as described 57 ...
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bioRxiv - Biophysics 2021Quote: The DNA coding for NRMTs from 82 bacterial and archaeal strains were cloned from gDNA (obtained from DSMZ) into pBXNHG3 or pBXC3H vectors (Invitrogen) using FX cloning (Geertsma and Dutzler ...
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bioRxiv - Microbiology 2021Quote: ... The mycelia of the tested strains were then collected and preincubated for 15 min with 200 nM MitoTracker Red CMRos (M7512, Invitrogen). After washing with phosphate-buffered saline (PBS) ...
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bioRxiv - Bioengineering 2021Quote: ... All plates were spread with 150uL of the yMM1456 (suc2Δ leu2Δ) strain with glass beads (Fisher Scientific, #11-312B 4mm), the beads were removed and the plate allowed to dry for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: The catalytic domain of the human ST6Gal I (residue range 132-406) was expressed in a BL21(DE3) Escherichia coli strain (Invitrogen), using a polycistronic Ptac vector containing the CyDisCo system (Gąciarz et al ...
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bioRxiv - Genetics 2019Quote: ... 10 pairs of testes from each transgenic strain were pooled to constitute a biological replicate for total RNA and protein extraction using TRI reagent (Ambion). RNA was reverse-transcribed using Superscript II (Invitrogen ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 3 wells from each strain were fixed with 10 µl of 16% paraformaldehyde (Fisher Scientific Cat. No. 50-980-487) and stored at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Using the DNA we again confirmed strain identity by sequencing the ITS region and nucleic acid quantity was measured using Qubit (ThermoFisher) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from RNA extracted from ~30 pairs of ovaries or testes dissected from 5-7 days-post-emergence (dpe) Liverpool strain adults using Trizol (Invitrogen). Primers LA1076 then nested with LA1352 (5’ ...
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bioRxiv - Microbiology 2024Quote: ... One fluorescent variant of aEPEC 1711-4 was obtained by transforming the wild-type strain with the recombinant plasmid pDP151 (Invitrogen), which encodes the fluorescent protein mCherry and confers resistance to ampicillin.
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bioRxiv - Biochemistry 2024Quote: ... Ligations of the restricted ORF fragments into the pYES2 vector were transformed into competent Escherichia coli TOP10™ strain (Invitrogen) and grown in Luria-Bertani (LB ...
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bioRxiv - Molecular Biology 2022Quote: ... derived from the MHOM/NP/02/BPK282/0 strain was maintained as promastigotes at 26°C in HOMEM medium (Gibco, ThermoFisher) supplemented with 20% Foetal Bovine Serum ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: Mouse embryonic cerebral cortices (strain C57BL/6N) were harvested at gestational day 14.5 (E14.5) and digested with Trypsin (Gibco, 15090-046). To obtain a single cell suspension the cells were mechanically dissociated with a glass Pasteur in neuronal culture medium (Neurobasal™ Plus Medium ...
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bioRxiv - Plant Biology 2023Quote: ... All direct assays and library screens were performed using the MaV203 yeast strain as described in the ProQuest manual (Invitrogen). Before screening the cDNA libraries ...
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bioRxiv - Molecular Biology 2023Quote: ... xanthus WT strain DK1622 and gene expression was quantified using the relative standard curve method (user bulletin 2; Applied Biosystems). 16S rRNA was used as the internal standard for each sample ...
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bioRxiv - Microbiology 2023Quote: ... Forty-three microliters of the cell suspension were applied in triplicate for each strain and allowed to dry in a MaxiSorp microtiter dishes (Nunc) plate overnight ...
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bioRxiv - Bioengineering 2023Quote: ... the bacterial toxin ccdB was cloned with the enzymes KpnI and NheI using a dedicated commercial ccdB-resistant strain (ThermoFisher); the generated plasmid LentiCRISPRV1-gRNA-TetR-intron-ccdB was used to obtain the KpnI/NheI digested backbone for lentiviral library ...
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bioRxiv - Microbiology 2023Quote: Two-day biofilm formation of Pseudomonas strains was determined by crystal violet staining using a 96-well plate lid with pegs extending into each well (Nunc-TSP lid ...
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bioRxiv - Plant Biology 2023Quote: ... All entry clones were propagated in the DH5 [strain of E.coli using a single gateway cloning system (Invitrogen, Cat# 12538120). The sense and antisense for STM were cloned under pG1090 inducible promoter (Zuo et al ...
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bioRxiv - Microbiology 2024Quote: Brachyspira pilosicoli strain P43/6/78 (ATCC #51139) was grown in Brain Heart Infusion (BHI) broth (Thermo Fisher Scientific, #CM1135B) containing 10% heat-inactivated fetal bovine serum and 0.2% glucose solution for four days in an anaerobic chamber (Coy Lab Products ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were derived from twenty-month old mice (C57BL/6 strain) and stimulated for 48 hours with CD3/CD28 Dynabeads (11452D, Gibco). The cells were then transduced with p-SIREN-sh lentivirus particles to target sestrins or with control p-SIREN scramble vectors ...
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bioRxiv - Microbiology 2024Quote: ... All ESKAPE strains were grown at 30-37°C using Luria broth/agar (BD Difco, Fisher Scientific #DF0145-17-0) for 15-24 h.
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bioRxiv - Neuroscience 2021Quote: ... the slides were washed and incubated with Donkey anti-Mouse IgG (H+L) 488nm and Donkey anti-Rabbit IgG (H+L) 546nm (ThermoFisher #A-21202, A-10040, respectively) for Figure 5a ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed and incubated with Donkey anti-Mouse IgG (H+L) 488nm and Donkey anti-Rabbit IgG (H+L) 647nm (ThermoFisher #A-21202, #A-31573, respectively) for Figure 5b ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were reduced with 2 µL of 200 mmol/L Tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Thermo Scientific; final concentration of 14.8 mmol/L) and incubated at room temperature for 30 min ...
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bioRxiv - Plant Biology 2019Quote: ... 2002) containing the 35S Cauliflower Mosaic virus promoter and eGFP gene using the Gateway® LR Clonase™ II kit (Invitrogen Corp., Carlsbad, CA). A single colony was used to amplify the [Gene-pK7FW2/pK7WGF2] plasmid and it was sequenced before transforming Agrobacterium (strain GV3101 ...
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bioRxiv - Microbiology 2019Quote: ... The number of JEV RNA copies present in BBB-crossing samples was determined by RT-qPCR using TaqMan® Fast Virus 1-Step Master Mix kit (Applied Biosystems®, 4444432) according to the manufacturer’s instructions ...