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Citations for Thermo Fisher :
4751 - 4800 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The whole tissue was homogenized in 4 mL of TRIzol (Thermo Fisher Scientific), ¼ processed according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... ANDS-labeled SCCs were resolved on BoltTM Bis-Tris 4-12% Gel (ThermoFisher) and visualized by fluorescence.
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bioRxiv - Immunology 2024Quote: ... Electroporation was performed using 4 mm gap cuvettes (Fisher Scientific Canada, Toronto, ON) and a Gene Pulser II instrument (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... 3T3-L1 cells were seeded into 4-well chambered slides (Thermo Fisher, 154534) and were grown and differentiated as described previously 20 ...
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bioRxiv - Molecular Biology 2024Quote: ... SEC fractions of interest were loaded on 4-20% Tris-glycine gel (ThermoFisher) and stained with Coommassie Blue.
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bioRxiv - Molecular Biology 2024Quote: ... SEC fractions of interest were loaded on 4-20% Tris-glycine gel (ThermoFisher) and stained with Coommassie Blue.
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bioRxiv - Microbiology 2024Quote: ... France) and DNA was counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) and polysaccharide content (wheat germ agglutinin labeled with fluorescein ...
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bioRxiv - Microbiology 2024Quote: ... then fixed with 2% paraformaldehyde (pH = 7.2-7.4, Fisher Scientific 30525-89-4) for 15m ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were stained with DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) (Thermofisher Scientific) in PBS for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and the concentration of RNA was measured with a QubitTM 4 Fluorometer (ThermoFisher) using the RNA Broad Range Assay (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... then maintained in culture for 4 days in growth medium (DMEM [Thermo Fisher Scientific 11960044] supplemented with 10% heat inactivated FBS [Thermo Fisher Scientific 10437028] ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant (1 ml at ~4-5 mg/ml) was incubated with 50 μl of superparamagnetic Dynabeads Streptavidin C1 (Invitrogen) and rotated overnight at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were incubated in primary antibody overnight at 4°C (rabbit anti-GFP 1:1000; Invitrogen A-6455 RRID:AB_221570), and in secondary antibody at 1:500 for 3 hours at room temperature goat (anti-rabbit Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2022Quote: ... at 4°C overnight followed by donkey anti-rabbit Alexa 488 secondary antibody (1:1000; A32766, Thermo Fisher Scientific), donkey anti-mouse Alexa 546 (1:1000 ...
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bioRxiv - Biophysics 2022Quote: ... Oocytes were injected with 0.1 to 10 ng cRNA of mASIC1a (volumes between 9 and 50 nL) and incubated for 1–4 days at 18 °C in Leibovitz’s L-15 medium (Gibco), supplemented with 3 mM L-glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were first incubated overnight at 4°C in rabbit anti-CALR (1:500, ThermoFisher, Waltham, MA, cat# PA3900) then goat anti-rabbit Alexa Fluor 488 (1:2000 ...
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bioRxiv - Immunology 2019Quote: ... loaded onto NuPAGE® 4-12% gels and run at 120 V in 1 X MES buffer (ThermoFisher Scientific). The separated proteins were transferred onto membranes using the iBlot® transfer system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Finally samples were counter stained for nucleus with 1 μM DAPI (4′, 6-diamidino-2′-phenylindoldihydrochloride; Thermo Fisher Scientific). Zeiss inverted Axio Observer fluorescent microscope (Zeiss ...
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bioRxiv - Neuroscience 2019Quote: ... at 4 °C followed by incubation the next day with goat anti-chicken antibody (647nm, A21449, ThermoFisher, 1:600) and goat anti-rabbit antibody (568 nm ...
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bioRxiv - Genomics 2020Quote: ... 5 mM MgCl2, 1 mM EGTA, 0.5 mM DTT, 0.05% Tween-20, 4 U/mL SUPERase-In [Thermo Fisher] ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated with primary antibodies (in 0.01 M PBS with 1% BSA and 0.3% Triton X-100) overnight at 4 °C and subsequently with corresponding secondary antibodies (1:1,000, Alexa-Fluor-conjugated, Invitrogen). DAPI was used to label the nuclei and sections were mounted with 75% glycerol ...
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bioRxiv - Biophysics 2020Quote: ... cells were previously fixed with paraformaldehyde 4% and permeabilized with Triton-X100 (0.5%) in PBS and stained with 1/200 anti-E-cadherin-FITC (Invitrogen).
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bioRxiv - Bioengineering 2021Quote: ... The resulting supernatant was centrifuged at 20,000 ×g for 1 hour at 4°C (Sorvall WX 100+ Ultracentrifuge, ThermoFisher) to pellet large EVs and the remaining supernatant was again centrifuged at 100,000 ×g for 1 hour at 4°C to pellet crude small EVs ...
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bioRxiv - Bioengineering 2020Quote: ... 1×1010 sEV were incubated with 3.8 µm aldehyde/sulfate latex 4% (m/v) beads (Molecular probes, Eugene, OR). Coated beads were then incubated with anti-human CD63 (eBioscience ...
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bioRxiv - Bioengineering 2021Quote: ... cells were live-dead stained with 2 µM calcein acetoxymethyl and 4 µM ethidium homodimer-1 (#L3224, Thermo Fisher) and imaged using fluorescent microscopy to compare cell density and viability between each silicone substrate ...
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bioRxiv - Bioengineering 2020Quote: ... 1-mm coronal sections from each tissue (4-mm for the brain) were homogenized in Trizol (Life Technologies, 15596) using a BeadBug (Benchmark Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 2 µg PAX2 and 4 µg shRNA in pLKO.1 backbone using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated overnight at 4 °C with primary antibody against ZO-1 (7.5 μg/ml, Thermo Fisher Scientific). Alternatively ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Chromosomes were counterstained for 20 minutes with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; ThermoFisher Scientific, D3571) in 2x SSC ...
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bioRxiv - Microbiology 2020Quote: ... Cell envelopes were stained by adding 5 µL of 1 mg/mL FM™ 4-64FX (Thermo Fisher Scientific) and non-viable cells stained with 1 µL of the LIVE/DEAD™ cell dye (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... AGS cells were transiently transfected for 24 h with the sgRNA-containing pX459 vector using polyethylenimine (PEI) at a 4:1 PEI:DNA ratio in Opti-MEM I Reduced Serum Media (OptiMEM; Gibco). Puromycin treatment (2 µg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... Tubes were incubated for 1 hour at 4°C and then protein A/G agarose magnetic beads (Thermo Scientific) were added for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: The lipophilic fluorescent membrane probe 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH) (Themo Fisher Scientific) was used to assess EV associated lipids ...
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bioRxiv - Cell Biology 2020Quote: ... The organoids were incubated overnight at 4◦C with a primary antibody against KI67 (Invitrogen #MA5-14520; 1:200) or MUC2 (Santa Cruz #sc-15334 ...
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bioRxiv - Neuroscience 2020Quote: ... TRA-1-60) in iPSCs was performed using the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Invitrogen™, A24881). iPSC colonies were fixed for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... islets were similarly incubated for 1 hr in imaging buffer at 37 °C with the addition of 2.5 μM Fluo-4 (Invitrogen). Islets incubated with Fluo-4 were washed three times with imaging buffer prior to imaging ...
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bioRxiv - Microbiology 2022Quote: ... lipid concentrations of FtsA fractions were determined by incubation of FtsA with FM 4-64FX (1.25 μg ml-1) (Invitrogen). Fluorescence intensity was measured with an Agilent Eclipse spectrofluorometer using an excitation wavelength of 565 nm and an emission wavelength of 745 nm (slit widths 5/10 nm) ...
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bioRxiv - Biochemistry 2022Quote: ... Sections were incubated overnight at 4 degrees with anti-ATF4 (10835-1-AP, ThermoFisher, Waltham, MA USA, RRID: AB_2058600) antibody at a 1:200 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary antibody O/N at 4°C (5% FBS, 0.5% Tx, 0.2% gelatine in PBS; GFP (1:500, ThermoFisher Scientific). Slices were then repeatedly washed with PBS-0.5% Tx ...
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bioRxiv - Biochemistry 2022Quote: ... the media in each plate was replaced with 40 μL of 1× Hank’s Balanced Salt Solution (HBSS) containing 20 mM HEPES pH 7.4 and 4× dilution of Fluo4 (Thermo Fisher). The assay plate was incubated at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were precleared by incubation for 1 hour at 4°C on a rotator with 10μl Protein G magnetic beads (ThermoFisher). The indicated antibody was incubated in 600μl IP buffer containing 1μl 10mg/ml acetylated BSA and 5μl Protein G magnetic beads for 4 hours with rotation at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... 10% dialyzed fetal bovine serum (PAA),4 mM L-glutamine (PAA),1% penicillin/streptomycin (100 IU/100 μg, Invitrogen). For Light Medium ...
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bioRxiv - Molecular Biology 2020Quote: ... Crosslinking was reversed by incubating the beads at 65°C for 4 hr in the presence of 0.3% SDS and 1 mg/ml of Proteinase K (Ambion). DNA was purified using Zymo ChIP DNA clean and concentrator kit (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: ... 4 x TaqMan® Fast Virus 1-Step Master Mix made up with molecular-grade nuclease free water (Ambion) to a final volume of 15 μl ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then incubated overnight at 4°C with a 1:500 dilution of rabbit anti-HA antibody (Invitrogen) in PBS 1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... 6.25 µl of 4 μM primers P1 and P2 and 1 µl of template DNA (Thermo Scientific, cat#F553S), and milliQ water up to 50 μl ...
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bioRxiv - Microbiology 2020Quote: ... BCR-expressing Ramos B cells were suspended at 4 million cells/mL in RPMI++ and labeled with 1.5 μM Indo-1 (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... falciparum NF54 parasites were cultivated at 4% hematocrit in human B+ blood in RPMI-HEPES supplemented with 0.25% Albumax 1 (Invitrogen) and human donor plasma type B+ ...
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4°C and with anti-rabbit IgG Alex Fluor 633 (ThermoFisher, Cat# A-21070, dilution 1:2000) for one hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... lysate from mock transfected cells was incubated at 4°C for 1 hour with Sepharose-G beads (Fisher Scientific), previously washed with 5% BSA in TBS-Triton buffer and preincubated with 2.5 μg anti-GFP antibody ...