Labshake search
Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... N2a cells were transfected with pSpCas9(BB)-Puro empty vector or vectors expressing each of the three gRNAs targeting the eIF4E3 exons (meIF4E3 gRNA exon 1, exon 2, exon 4) using Lipofectamine 2000 (Invitrogen). pSpCas9(BB)-Puro was a generous gift from Dr ...
-
bioRxiv - Developmental Biology 2021Quote: ... The iPSCs were dissociated (DD0) after 4-5 days of maintenance using 0.25% trypsin / 1 mM EDTA (Thermo Fisher Scientific) at 37°C for 2 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... and 8 mM MgSO4 pH 7.0 (2x PHEM buffer) containing 1% Triton X100 for 5 min and then fixed in 4% (v/v) paraformaldehyde (ThermoFisher) in 2x PHEM buffer at 37°C ...
-
bioRxiv - Neuroscience 2021Quote: ... was reconstituted by submerging the vial at 4°C over night and was diluted 1:5 in ice-cold knock-out DMEM (Cat.-No. 10829, Gibco) until material was evenly dispersed ...
-
bioRxiv - Plant Biology 2020Quote: ... Quality and quantity of bulked DNA was checked by running a DNA sample on an agarose gel (1%) and final DNA concentration determined using the Quibit-4 Fluorometer (dsDNA BR assay, Q32850, Thermofisher).
-
bioRxiv - Cancer Biology 2020Quote: ... All cell lines were maintained in DMEM (25 mM Glucose, 4 mM Glutamine, 1 mM Sodium Pyruvate) supplemented with 10% Fetal Bovine Serum (FBS, Gibco) and 50 units/mL Penicillin/Streptomycin (Gibco ...
-
bioRxiv - Immunology 2022Quote: Bacteria in PBS were first stained for 1 h at 37°C with 4 μM Oregon Green 488-X succinimidyl ester (Invitrogen) followed by 20 μg/ml CypHer5E (Cytiva ...
-
bioRxiv - Biochemistry 2022Quote: ... C6 cells were grown and maintained in High Glucose DMEM (25 mM glucose, 1 mM pyruvate, 4 mM glutamine; #12800017, Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin ...
-
bioRxiv - Cell Biology 2022Quote: ... Skeletal muscle were stained with Myosin 4 monoclonal antibody (MF20, MyHC-2) (1:200; Thermo Fisher Scientific, Rockford, IL, USA) and Laminin gamma 1 Antibody (A5 ...
-
The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
-
bioRxiv - Microbiology 2022Quote: ... Enteroids were then washed with PBS three times with the addition of 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (1:5000) (Invitrogen) for the final 10 min wash ...
-
bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum) for 1 hr at 4°C and then with a primary antibody anti-GFP (Invitrogen, catalog # G10362 ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then incubated with anti-IgM antibody (1/100) (Jackson Immuno Research) for 30 min at 4°C and mounted in Fluoromount-G with DAPI (Invitrogen). Fluorescence was detected using an inverted confocal microscope ...
-
bioRxiv - Microbiology 2022Quote: ... Total lipids were extracted by resuspending washed cell pellets in 1 mL HPLC grade methanol (A454-4, Thermo Fisher Scientific), transferring to a glass vial with PFTE cap ...
-
bioRxiv - Neuroscience 2022Quote: ... and spinal cord medium (MEM with Glutamax [Gibco] supplemented with 4 mg/ml Albumax [Gibco], 1 mM pyruvate [Sigma], 100 units/ml Penicillin and 100 μg/ml Streptomycin [Gibco]), as previously described (Dumoulin et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... The plasmids were mixed with 100 μl of PEI (4:1 v/w PEI:DNA ratio) in 500 μl of pre-warmed OptiMEM II reduced serum medium (Gibco, 1985070), after 10-15 min incubation at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... the culture was fixated by removing the remaining medium and adding 1 mL of a 4% paraformaldehyde (PFA, Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 1hr before being incubated overnight at 4°C in and primary antibodies (1:1000 mouse anti-V5 Tag monoclonal Antibody (Invitrogen) and anti-Actin monoclonal antibody (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: ... the sections were washed 3 times for 20 minutes each time in 1x PBS/0.3% Triton X-100/10% NDS at room temperature and incubated overnight at 4°C with secondary antibodies (Thermo Fisher Scientific Cat# A-21206, RRID:AB_2535792, 1:500; Thermo Fisher Scientific Cat# A-21203 ...
-
bioRxiv - Plant Biology 2023Quote: ... 4 μl of the DNAseI reaction was mixed with 1 μl of oligo(dT)18 0.5 μg/μl (Thermo Scientific), and incubated at 65°C for 5 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... overnight at 4°C followed by incubation with donkey anti-goat Alexa Fluor 594 secondary antibodies (1:200, A-11058; Invitrogen) and rabbit anti GFP (1:100 ...
-
bioRxiv - Neuroscience 2022Quote: ... they were incubated for 24 hours at 4 °C with Alexa Fluor 594-conjugated donkey anti-goat IgG (1:300, A-11058, Invitrogen), Cy3-conjugated goat anti-rabbit IgG (1:500 ...
-
bioRxiv - Neuroscience 2022Quote: ... the tissues were washed five times with PBS and then permeabilized for 1 hour with 0.2% TritonX-100 supplemented with 4% goat serum (Thermo Fisher). The permeabilization solution was also used to generate a primary antibody solution ...
-
bioRxiv - Neuroscience 2022Quote: ... before being incubated 72hours at 4°C with the following primary antibodies: chicken anti-GFP (1:1000, Life Technologies, #A10262) and rabbit anti-FMRP (1:500 ...
-
bioRxiv - Neuroscience 2022Quote: ... WAKO) overnight at 4 °C followed by detection with Alexa 488 conjugated anti-rabbit secondary antibody (1:1000, Life Technologies), and Alexa 594 conjugated anti-rat secondary antibody (1:1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568, 1:200, Invitrogen, A11041 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Overnight incubations (4°C) of the sections were carried out with mouse monoclonal anti- proliferating cell nuclear antigen (PCNA) (1:200, Invitrogen). Control sections were incubated with a mouse IgG Isotype Control (1:200 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 48 hpf embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) in 10% DMSO ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were centrifuged at 500 ×g for 5 minutes at 4°C and washed and resuspended with ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS) ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by a goat anti-rabbit Alexa Fluor 546 secondary antibody (1:1000; overnight at 4 °C; # A-11035, Invitrogen). Sections were mounted in a medium containing 50 % glycerol ...
-
bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated rotating for 1 h at 4 ºC on 1.25 mL bed of HisPur Ni-NTA agarose beads (ThermoFisher Scientific), pre-equilibrated in lysis buffer ...
-
bioRxiv - Cancer Biology 2024Quote: ... and incubated overnight at 4°C in a humified chamber with anti-AKAP12 antibody (Thermo Fisher #PA5-21759, 1:200) or anti-Ki67 (CST #9449 ...
-
bioRxiv - Cell Biology 2024Quote: ... Myoblasts were cultured in growth medium consisting of 1 vol 199 Medium/4 vol DMEM (Life Technologies, Carlsbad, CA, USA) supplemented with 20% fetal calf serum (Life technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... both at 1:300 dilution and mounted with ProLong Gold antifade reagent with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen). Confocal microscopy (Olympus FLUOVIEW FV3000 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Glycoproteins were resolved by SDS–PAGE (approx. 1×107 cell equivalents/lane) on NuPAGE bis-Tris 4–12% gradient acrylamide gels (Invitrogen) and transferred either to nitrocellulose or PVDF membranes (Invitrogen) ...
-
bioRxiv - Pathology 2024Quote: ... at 4 °C overnight followed by an Alexa Fluor 594-conjugated secondary antibody (1:500) (A-11012, Thermo Fisher Scientific). Immunofluorescence staining images were acquired using the Pannoramic Scanner software (v3.0.3.139795 RTM) ...
-
bioRxiv - Neuroscience 2024Quote: ... denatured (70°C for 10min) protein samples (1 µg/µl) were loaded onto a NuPAGE 4-12% Bis-Tris Gel (Invitrogen) along with Chameleon 800 pre-stained protein ladder (LI-COR) ...
-
bioRxiv - Developmental Biology 2024Quote: ... the samples were washed extensively in PBS and then incubated overnight at 4°C in blocking solution with the appropriate concentration of secondary antibodies diluted at 1:500 and with DAPI (Invitrogen) diluted at 1:1000 ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were purified at 4°C by the batch method over 1 mL Ni-NTA resin according to the manufacturer’s instructions (HisPur, Thermo Scientific). All buffers were 50 mM NaPO4 ...
-
bioRxiv - Immunology 2024Quote: ... the membranes were probed overnight at 4°C with anti-mouse CASP-1 (Cat# 14-9832-82; Thermo-Fisher Scientific) or anti-mouse mature IL-1β (Cat# 63124S ...
-
bioRxiv - Cell Biology 2024Quote: ... at 4°C o/n and then with 40 μL of pre-blocked (1 mg/ml BSA) Dynabeads protein A (ThermoFisher). Beads were sequentially washed with Wash buffer 1 (20 mM Tris HCl pH 8.1 ...
-
bioRxiv - Biophysics 2023Quote: ... incubated in 10 mg/mL sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC, Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... then incubated overnight at 4° C in a 1:1000 dilution of secondary antibody (Thermofisher goat anti-rabbit AF546, 11010) in PBT+ ...
-
bioRxiv - Cell Biology 2023Quote: ... The Sca-1+-VPCs were maintained in stem cell culture medium and passaged every two days at a ratio of 1:4 by Trypsin-EDTA (0.25%) phenol red (ThermoFisher, UK). Up to 5 passages were used in this study ...
-
bioRxiv - Genetics 2023Quote: ... [DNA : Lipofectamine = 1 μg : 4 μL] in 300 μL of Opti-MEM I Reduced Serum Medium (#31985062; Thermo Fisher Scientific) and then plated in 2 mL of culture medium onto a six-well plate.
-
bioRxiv - Cell Biology 2023Quote: ... were generated by mixing the appropriate pTT5 expression vector with transfection reagent (ratio of 1 µg vector : 4 µg PEI MAX) in F17 Expression Medium (Gibco). Complexes were incubated for 15 min at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by an overnight incubation at 4 °C with 20 μg ml-1 laminin (Thermo Fisher Scientific, 23-017-015). After being thoroughly washed with PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... in blocking buffer overnight in 4°C and stained with secondary AlexaFluor633 donkey anti-goat antibodies (Invitrogen, #A21082; 1:200) for 1 hour at RT ...
-
bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...