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Citations for Thermo Fisher :
4651 - 4700 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Levels of GTP-bound Rac1 and Cdc42 were determined using either active Rac1/Cdc42 pull-down and detection kit (Cat# 16118/19, ThermoFisher Scientific) following the of the manufacturer’s instructions and buffer composition or purified GST-Pak1-PBD fusion beads ...
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bioRxiv - Developmental Biology 2024Quote: ... detection was performed on GOCs according to the Click-iT EdU Alexa Fluor 488 Imaging Kit manufacturer’s instructions (Molecular Probes, C10337). Briefly ...
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bioRxiv - Genetics 2024Quote: ... 1 μg RNA was reverse transcribed using the TaqMan Reverse transcription kit and gene level detection performed using SYBR Green Master Mix (all Thermo Scientific). Relative expression was normalized to GAPDH and ACTB ...
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bioRxiv - Developmental Biology 2023Quote: EdU detection was conducted with (Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 647 dye, Invitrogen, C10340) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Samples were visualized using horseradish peroxidase coupled to appropriate secondary antibodies with enhancement by an ECL detection kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... the replication detection was carried out using the Click-iT EdU Cell Proliferation Kit for Imaging (AlexaFluor 555 dye; Invitrogen, USA) following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2023Quote: ... NAE staining was followed by 5-ethynyl-2⍰-deoxyuridine (EdU) detection using Click-iT Edu Alexa Fluor 488 or 594 Imaging kits (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2023Quote: ... The membrane was incubated with streptavidin-conjugated horseradish peroxidase and then with reagents of the Chemiluminescent Nucleic Acid Detection Module Kit (ThermoFisher, 89880). The membrane was then scanned with the use of a ChemiDoc XRS+ System (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... Detection of EdU was accomplished using the Click-iT Plus EdU Alexa Fluor 488 or 647 Imaging Kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... cDNA synthesis was performed with Sensifast cDNA synthesis kit (Meridian Bioscience) and Quantitative PCR (qPCR) on CFX384 Real-Time PCR Detection System using DyNAmo Flash SYBR Green qPCR Kit (ThermoFisher Scientific). Relative quantification of gene expression was performed by the 2−ΔΔCt method using Gapdh as the housekeeping gene for normalization ...
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bioRxiv - Cell Biology 2022Quote: ... the metabolic activity of viable cells was tested by a colorimetric dimethyl-thiazole-diphenyltetrazolium bromide (MTT) assay (cat. no. M6494, Invitrogen). Briefly ...
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bioRxiv - Microbiology 2022Quote: Cell toxicity due to the compound treatment was determined using the standard colorimetric MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The colorimetric reaction was then measured at 540 nm using a Multiskan Go microplate reader (Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Biochemistry 2022Quote: ... The dried peptides were resuspended in 200 mM EPPS pH 8.5 and quantified by bicinchoninic acid assay using the quantitative colorimetric peptide assay from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Sep-Pak Vac tC18 solid phase extraction cartridges (Waters) and the concentration determined using the Pierce quantitative colorimetric peptide assay (ThermoFisher). Peptides were dried by vacuum centrifugation.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The PCR product was used as the template for the dsRNA synthesis with a high yield transcription kit MEGAscript T7 Kit (Thermo Fisher Scientific: Pittsburgh, PA). The dsRNA was purified using a GeneJET Gel extraction Kit (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Lung or liver total RNA was extracted with the Nucleospin RNA II kit (Macherey Nagel, Düren, Germany) and reverse-transcribed with the High-Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA). The cDNA was amplified using SYBR-Green-based real-time PCR on a Quantstudio™ 12K Real-Time PCR System Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Isolation of sarcoplasmic and sarcolemmal protein were initially isolated from muscle tissue using a high-fidelity membrane protein extraction kit according to the manufacturer’s recommendations (Mem-PRE Plus Membrane Protein Extraction Kit; Thermo Fisher Scientific; Waltham, MA, USA). This process yielded solubilized sarcoplasmic and sarcolemmal protein isolates.
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bioRxiv - Cell Biology 2022Quote: Cells were grown on 10 cm plates and total RNA was extracted using the Trizol reagent (Invitrogen, 15596026). Cells were rinsed twice in PBS containing 0.1 mM CaCl2 and 1.0 mM MgCl2 ...
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bioRxiv - Pathology 2020Quote: Three separate 10 µL RT-qPCR reactions were set up in a 384-well Barcoded plate (Thermo Scientific) for either the N1 ...
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bioRxiv - Cell Biology 2019Quote: ... 15 mg/mL) and photon counts were obtained after 10 minutes using a plate reader (VarioSkan Lux, Thermofisher), with 1 second acquisition per well ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We ran 10 plates (384-well format) on QuantStudioTM 12K Flex Real-Time PCR System (Thermo Fisher Scientific). Each 10 μL reaction contained 200 nM of each primer ...
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bioRxiv - Cell Biology 2021Quote: ... Suspended myofibers were cultured in 60 mm horse serum-coated plates in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 2% chicken embryo extract (Accurate Chemical ...
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bioRxiv - Systems Biology 2021Quote: ... Mixtures were supplemented with Alexa-488 at 10 µM and plates were sealed with adhesive qPCR seals (ThermoFisher).
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bioRxiv - Biochemistry 2022Quote: ... 5 × 105 landing pad cells expressing hACE2 with mNG21-10 were seeded in 6-well plates (Fisher Scientific). The cells were then then incubated at 37 °C with 5% CO2 for 15 mins to allow seeding ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293-T cells were seeded into 10 cm plates with DMEM containing 0.5 mg/mL geneticin G418 (Thermofisher). Once the cells reached approximately 80% confluence ...
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bioRxiv - Microbiology 2022Quote: ... 4×104 conidia of each isolate was evenly spread on a solidified RPMI plate (1.5% agar) and 10 μl of 0.8 mg/mL voriconazole or 3.2 mg/mL itraconazole (Acros Organics) was added to a Whatman 6 mm antibiotic assay disc which was placed in the middle of the plate ...
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bioRxiv - Neuroscience 2019Quote: ... The plate was then washed and 100 μL of CSF (diluted 1:10 in Blocker Casein (Thermo Scientific) in Tris-buffered saline ...
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bioRxiv - Cell Biology 2019Quote: 80% confluent 10 cm plates of Plat-E cells were transfected by using Lipofectamine 2000 (Thermo Fisher Scientific) with 10 μg retrovirus vectors in additional 1.5 ml Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2021Quote: ... Hepatocytes were allowed to attach for 90 minutes on collagen-coated plates in RPMI containing 10% FBS (Gibco), followed by overnight starvation in serum-free medium before experiments (Lipogenesis and β-oxidation assay).
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bioRxiv - Synthetic Biology 2021Quote: ... 10 µL was then added to each well of an assay ready plate using a Multidrop Combi (ThermoFisher). Percent inhibition was calculated on a scale of 0% (i.e. ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 x 106 WT HEK293T cells were plated in 10 cm plates and transfected using Lipofectamine 2000 (Invitrogen) with the following plasmids expressing V5-SBP fusions ...
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bioRxiv - Microbiology 2021Quote: ... A 10 μL drop from each dilution was transferred to a Mueller-Hinton plate (Thermo Fisher, Waltham, MA) and incubated overnight in ambient air at 35°C (58) ...
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bioRxiv - Immunology 2020Quote: ... For poly(I:C) stimulation cells were transfected in 6-well plates with 10 μg polyI:C using Lipofectamine 2000 (Invitrogen) for 2 h before imaging ...
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bioRxiv - Genomics 2022Quote: ... Plates were incubated for 10 minutes with PBS with Hoechst 33342 (1:500 dilution) (#134406, Thermo Fisher Scientific) and then washed 3x with PBS ...
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bioRxiv - Cell Biology 2024Quote: We seeded HEK293T cells at 2.5 × 10^6 cells per 15 cm plate in DMEM (Thermo Fisher 11965118), enriched with 10% FBS (Atlanta Biologicals S11150H) ...
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bioRxiv - Microbiology 2024Quote: ... 1 in 10 serial dilutions solutions were created using sterile PBS in a 96 well plate (ThermoFisher Scientific). Bacterial counts (CFU/mL ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in tissue culture treated plates containing Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC # 30-2002) containing 10% fetal bovine serum (Gibco) until 60% confluent after which cells were transfected using Lipofectamine 2000 (Invitrogen # 11668019 ...
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bioRxiv - Developmental Biology 2023Quote: Mouse ESCs from 10 cm culture plates were harvested and disassociated using Trypsin EDTA (Thermo Fisher Scientific, 25200056), washed twice in PBS (cells pelleted by centrifugation 300 rpm ...
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bioRxiv - Synthetic Biology 2023Quote: ... Three 10 μL aliquots of each reaction mixture were transferred to a 384-well plate (Thermo Scientific 142761), covered with a seal (VWR 60941-078) ...
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bioRxiv - Immunology 2023Quote: ... 10% H2/N2 (BOC) for 3 days on blood agar plates containing 7% laked horse blood (Thermo Scientific) and the Campylobacter selective supplement “Skirrow” containing the antibiotics trimethoprim ...
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bioRxiv - Developmental Biology 2023Quote: ... The Six2+ cells were directly FACS sorted into a 96 well plate supplemented with 10% FBS/ Dulbecco’s modified Eagle’s medium (DMEM,Gibco) in the presence of 4 µM DMSO (Thermo) ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... Cancer cells (5 × 103 cells) were seeded in 96-well plates in DMEM supplemented with 10% FBS (Gibco) and 1% antibiotic-antimycotic (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... Horizon Discovery) using the DharmaFECT reagent (54 μL/10 cm plate) in OPTI-MEM reduced serum media (ThermoFisher) according to the manufacturer’s instructions (Horizon Discovery) ...
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bioRxiv - Microbiology 2023Quote: ... qPCR was conducted in 384-well plates using the SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience) on the QuantStudio 5 (384-well plate) qPCR cycler (Applied Biosystems, Foster City, CA, USA). The qPCR program encompassed 40 cycles ...
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bioRxiv - Genetics 2020Quote: ... PCR amplification of genomic DNA was performed using Phusion™ Green Hot Start II High Fidelity DNA Polymerase with GC Buffer in presence of 10% of DMSO (ThermoFisher). Big dye sequencing chemistry was used to sequence the PCR products in both directions using the ABI 3500xL Genetic analyser (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... were obtained from the UC Berkeley cell culture facility and were cultured in DMEM (Gibco-Thermo Fisher 1165092 high glucose, +L-glutamine,10% FBS, with the addition of 100 U/ml Pen/Strep-Gibco). Cells were seeded at 5000 cells/ cm2 for passaging ...
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bioRxiv - Cell Biology 2022Quote: ... and C2C12 cells (CRL-1772, ATCC) were cultured in modified DMEM (high glucose; Nacalai Tesque) containing 10% FBS (qualified, Thermo Fisher), 4 mM L-glutamine ...
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bioRxiv - Cell Biology 2020Quote: ... Cell suspensions were transferred to tubes containing MEF medium (DMEM-high glucose supplemented with 10% FBS, 50 U/ml penicillin and 50 mg/ml streptomycin (Invitrogen, Carlsbad), and 2 mM GlutaMAX ...
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bioRxiv - Molecular Biology 2019Quote: HEK-293T cells (293T/17, ATCC) were maintained in high-glucose DMEM supplemented with 10% fetal bovine serum (FBS, Life Technologies). U2OS cells (ATCC ...