Labshake search
Citations for Thermo Fisher :
4601 - 4650 of 7736 citations for Recombinant Human CLEC3B His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen, catalog no. AM2694), and the resulting RNA/DNA was tested for viral copies by qRT-PCR ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were then co-transfected with recombinant plasmid (300 ng) together with miR-544-3p mimic or negative control mimic (50 nM) using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) and Lipofectamine RNAimax (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2022Quote: ... at a bead-to-cell ratio of 1:1 in the presence of recombinant IL-4 (Fisher Scientific, #204-IL-010, 10 ng/ml) and anti-IFN-γ antibody (10 μg/ml) ...
-
bioRxiv - Microbiology 2019Quote: ... Purified cells were cultured in a tissue culture flask containing 20 mL RPMI 1640 supplemented with recombinant murine Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) (10 ng/mL) (BioSource, Invitrogen cytokines & signaling, Camarillo, CA). At day 3 of the culture ...
-
bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
-
bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen, AdenoPACK 20, Vivapure; Sartorius AG).
-
bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... were prepared from 10 to 11-day-old chicken embryos (SPF eggs, VALO, Cuxhaven, Germany) using recombinant trypsin (Tryple TM, Thermo Fisher Scientific, Planegg, Germany) and maintained in VP-SFM medium ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were replated onto glass coverslips at day 12 of the differentiation in N2/B27 medium supplemented with four recombinant growth factors at 25ng/ml (BDNF; ThermoFisher, NT3, NGF, GDNF; Peprotech). CHIR90221 was included for 4 further days ...
-
bioRxiv - Biochemistry 2023Quote: ... and the bound Arr3_ýC•IP6–Fab7 complexes incubated with HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 1 μg/ml of recombinant Tat using a commercial transfection reagent Pro-ject Protein Transfection Reagent Kit (Thermo Scientific Cat. No. 89850) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
-
bioRxiv - Cell Biology 2024Quote: Organoids were treated from day 4 through day 11 for EN1 and day 7 through day 11 for EPC2 with or without 10 ng/mL recombinant IL-13 (200-13, Thermo Fisher Scientific, Waltham, MA), reconstituted in phosphate-buffered saline (PBS ...
-
bioRxiv - Synthetic Biology 2021Quote: ... CD3+ T cells were activated with anti-human CD3/CD28 magnetic Dynabeads (CTS, ThermoFisher) in a 1:1 ratio with 100 U mL-1 of IL-7 and 10U mL-1 IL-15 (R&D Systems ...
-
bioRxiv - Cancer Biology 2021Quote: ... FAM-labeled target primer and endogenous human ACTB control (beta actin) (Thermo Fisher Scientific) were mixed with master mix and template ...
-
bioRxiv - Microbiology 2020Quote: ... Human embryonic stem cell (hESC; H9)-derived neural stem cells (NSC) (Thermo Fisher Scientific) were cultured ...
-
bioRxiv - Microbiology 2020Quote: A549 human epithelial cells (ATCC ref# CCL-185) were maintained in F12K media (Gibco) supplemented with 1x GlutaMax (Gibco ...
-
bioRxiv - Cancer Biology 2021Quote: ... AlexaFluor 488 conjugated anti-human/mouse CD44 antibody (1:50 dilution, Molecular probes, A25528) was directly added into the cell culture medium of the cells to be stained ...
-
bioRxiv - Cell Biology 2020Quote: ... Taqman probes targeting human GAPDH (Hs02758991_g1) and IFIT1 (Hs03027069_s1) were purchased from Life Technologies. qRT-PCR data were collected on a StepOnePlus™ Thermal Cycler (Thermo Fischer Scientific ...
-
bioRxiv - Immunology 2021Quote: Membranes were coated with an anti-human IgG capture antibody (Thermo Fisher Scientific, A18813). Cells were then added to the plate in duplicate in 2 fold serial dilutions and incubated for 24hrs prior to detection using an alkaline phosphatase-linked anti-human IgG detection antibody (Jackson ...
-
bioRxiv - Genetics 2021Quote: ... donkey-anti-rabbit and mouse-anti-human IgG secondary antibodies (Molecular Probes, 1:1000), or NbALFA-Atto647 (NanoTag Biotechnologies ...
-
bioRxiv - Genomics 2021Quote: ... Human K562 erythroleukemia cells were cultured in Roswell Park Memorial Institute (RPMI, Life Technologies) 1640 medium supplemented with 10% FBS ...
-
bioRxiv - Developmental Biology 2021Quote: ... or of the rabbit anti-Human Rac1 polyclonal antibodies (1:40, Thermofisher PA1-091) were added in 1 % BSA-PBS and incubated O/N at 4°C ...
-
CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Total RNA was isolated from human desmoid tumor cell lines using Trizol reagent (Invitrogen) according to the manufacturer instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... Primed human ESC and iPSC cells were harvested with 0.25% trypsin-EDTA solution (Invitrogen), and cells resuspended in PBS were transfected with 75μg DNA constructs (Gene Pulser Xcell System ...
-
bioRxiv - Microbiology 2022Quote: ... human foreskin fibroblast (HFF) monolayers were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplemented with 25 mM glucose (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2021Quote: ... slides were incubated with Alexa Fluor 488 goat anti-human IgG(H+L) (Invitrogen) at 1:500 (in 3% BSA/PBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... except for human XBP1S that was assessed using SYBR green master mix (Thermofisher Scientific) using primers in Supplementary Table 3.
-
bioRxiv - Molecular Biology 2021Quote: ... Stable integration of CLIP-UPF1SL into human Flp-InTM T-RExTM-293 cells (Invitrogen) and subsequent maintenance of this stable line was previously described (Kishor et al ...
-
bioRxiv - Genomics 2020Quote: The THP1 (human acute monocytic leukemia derived; ATCC number TIB-202 purchased from Thermofisher) cell line[18] was regularly maintained in RPMI 1640 medium containing GlutaMAX™ ...
-
bioRxiv - Genomics 2020Quote: ... Samples passing quality control were analyzed using the human Clariom D Assay (ThermoFisher Scientific) and analyzed using Transcriptome Analysis Console (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2019Quote: ... and stained with Alexa 647-conjugated anti-human IgG (H+L) antibody (Thermo Fisher) for 1h at 4°C ...
-
bioRxiv - Pathology 2020Quote: ... SMCs were transfected with control siRNA or siRNA targeting the human calpain-2 (Ambion - Silencer Select validated siRNA ...
-
bioRxiv - Cell Biology 2020Quote: ... HEK293 human embryonic kidney cells were cultured in Dulbecco’s modified Eagle medium (DMEM, Invitrogen), containing 10% FBS ...
-
bioRxiv - Biophysics 2019Quote: Homo sapiens SUR1 and human Kir6.2 were subcloned into pcDNA4/TO (Invitrogen; Carlsbad, CA) for expression in HEK-293T cells ...
-
bioRxiv - Genomics 2019Quote: ... dataset (which used an Affymetrix platform, [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array); the default importer in BRB-ArrayTools was used to collate the data from .cel files ...
-
bioRxiv - Immunology 2019Quote: ... anti-human CD3 conjugated to FITC (clone OKT3, cat. no. 11-0037-41, Invitrogen), anti-human CD8 conjugated to Pacific Blue (clone 3B5 ...
-
bioRxiv - Immunology 2019Quote: ... Cortactin expression was assessed using rabbit anti-human cortactin antibody (PA5-27134; Life Technologies) stained in conjugation with goat anti-rabbit Cy3 (Life Technologies) ...
-
bioRxiv - Immunology 2019Quote: ... and the ELISPOT plate was incubated with biotinylated goat anti-human IgG Fc (Invitrogen), followed by HRP-conjugated avidin (Vector Laboratories ...
-
bioRxiv - Cancer Biology 2020Quote: ... Then the cells were stained with AlexFuor488-conjugated goat anti human Fc (Life technologies) at RT for 30 min and analyzed by flow cytometry ...
-
bioRxiv - Genomics 2019Quote: ... We used the Genome-Wide Human SNP Array 6.0 (Affymetrix, Santa Clara, CA, USA) for genotyping single nucleotide polymorphisms (SNPs) ...
-
bioRxiv - Immunology 2021Quote: ... coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher, #H17000) in 0.1 M carbonate buffer ...
-
bioRxiv - Immunology 2021Quote: ... Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific) and cultured in DMEM medium (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2021Quote: ... followed by the secondary goat anti-human HRP-conjugated antibody (1:4000, A18805, Invitrogen). Stained proteins were detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
-
bioRxiv - Biochemistry 2020Quote: Human GCDH cDNA (clone ID 3142978) in pOTB7 vector was obtained from Thermo Fisher Scientific Open Biosystems repository (now part of Horizon Discovery) ...
-
bioRxiv - Biochemistry 2020Quote: ... 7.5 μg/mL human IgG and 50 μg/mL BSA-biotin (Thermo Fisher Scientific) were also printed to serve as a printing control and “intra-well signal normalizer” ...
-
bioRxiv - Biochemistry 2020Quote: ... and negative selection using the Dynabeads Untouched Human CD4 T Cells kit (Invitrogen 11346D) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: Human embryonic kidney (HEK)293T cells (ATCC, CRL-11268) were grown in DMEM (Gibco) plus 10% heat-inactivated fetal calf serum (FCS ...
-
bioRxiv - Biophysics 2021Quote: ... CD4+ T cells were stimulated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) and cultured in AIM V Medium (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS (Gibco ...