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Citations for Thermo Fisher :
4551 - 4600 of 7736 citations for Recombinant Human CLEC3B His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The AML sample underwent human CD3 depletion using dynabead CD3 depletion kit (Invitrogen) prior to injection ...
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bioRxiv - Cell Biology 2024Quote: ... The following Taqman probes were used: human ACTB (Thermo Fisher Scientific, 4331182-Hs01060665_g1), human MCU (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: Human foreskin dermal fibroblasts were cultured in DMEM (Thermo Fisher, Cat No: 11965092) with 10% FCS and 1% Pen/Strep at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: Suspension adapted human embryonic kidney cells (HEK293F) were acquired directly from Thermo Fisher Scientific as an authenticated product maintained according to the manufacturer’s guidelines in either FreeStyle 293 Expression medium (Cat #12-338-018 ...
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bioRxiv - Cancer Biology 2024Quote: ... siRNA oligonucleotides (20 nM) targeting eIF4B (Human: s4573 & s4574; Mouse: s93804 & s93805 ThermoFisher) were transfected using Lipofectamine RNAiMax (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... Primary human T cells were cultured in AIM-V medium (Invitrogen 12055-083) supplemented with 5% Human serum (Sigma H4522-100ML) ...
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bioRxiv - Cancer Biology 2024Quote: ... A549 human lung adenocarcinoma cells (CCL_185, ATCC) were cultured in DMEM (Gibco, #41965039), and supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... human stool samples were diluted 1:10 in DEPC-treated water (Life Technologies), vortexed ...
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bioRxiv - Microbiology 2024Quote: Human embryonic kidney (HEK)293T (ATCC CRL-11268) and FreeStyle 293F (Life Technologies) cell lines were used for recombinant protein productions ...
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bioRxiv - Immunology 2024Quote: Human serum derived macrophages were lifted using TrypLE (5 mL, Thermo Fisher Scientific). Once lifted ...
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bioRxiv - Cell Biology 2024Quote: ... and a human proteome database (UniProt) was screened with SEQUEST (Thermo Fisher Scientific). The normalization node was added to the processing workflow ...
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bioRxiv - Immunology 2024Quote: ... IL-1β was evaluated using an IL-1 beta Human ELISA Kit (Invitrogen). Neutrophils (3 × 105/well ...
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bioRxiv - Immunology 2024Quote: Human and murine B cells were lysed in RIPA buffer (Thermo Fisher, #89900) supplemented with protease inhibitors (Roche ...
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bioRxiv - Immunology 2024Quote: ... Isotype-specific antibodies were detected with goat anti-human IgG (1/1000, Invitrogen) or IgM (1/2500 ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were activated with DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher, 11131D) at a 1:1 cell-to-bead ratio and cultured in 24-well plates with 1 million cells per mL at 37◦C and 5% CO2 overnight.
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bioRxiv - Immunology 2024Quote: ... in combination (each at 100ng/mL) or with Dynabeads human T-Activator (ThermoFisher) anti-CD3/CD28 beads (at a 1:1 bead/cell ratio) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... followed by secondary staining with a Goat anti-human IgG Fc (ThermoFisher A18818). Individual bEFNB2 expressing cells were then sorted into individual wells of a 96 well plate on a Sony MA900 and gated to include only live ...
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bioRxiv - Molecular Biology 2024Quote: ... the CECs were resuspended in stabilisation media (containing Human Endothelial-SFM (Life technologies) supplemented with 5% foetal bovine serum (FBS) ...
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bioRxiv - Immunology 2024Quote: ... ELISA plates were coated with anti-human IgG (ThermoFisher 88-50550-88, 1X) and blocked overnight with 2X assay buffer (Invitrogen 88-50550-88 ...
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bioRxiv - Immunology 2022Quote: ... The residual amount of endotoxin in the recombinant S100A4 preparation was examined using a Pierce™ Chromogenic Endotoxin Quant Kit (Thermo Fisher Scientific). Mice were anesthetized using isoflurane prior to immunization ...
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bioRxiv - Immunology 2021Quote: ... Purified digested DNA fragments were cloned into human Igγ1- and Ig κ-/ Igλ-expressing vectors 38 and recombinant IgG1 antibodies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described 39 ...
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bioRxiv - Immunology 2019Quote: ... the wild-type and mutant (C302/305R) recombinant Mul1 proteins were produced in Sf9 cells using the Bac-to-Bac Baculovirus expression system (Invitrogen, Carlsbad, CA, USA) and purified by a nickel-chelating resin (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: The interaction of the NAB1 promoter fragments with recombinant LCRF-SBP (rLCRF-SBP) was analyzed by using the LightShift®Chemiluminescent EMSA Kit (Thermo Scientific™), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: In vitro binding experiments were performed with recombinant GST fusion proteins purified from E.coli and immobilized on glutathione-Sepharose 4B beads (Life Technologies #17-0756-01). Purification of GST fusion proteins was performed as described (Ebnet et al. ...
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bioRxiv - Biochemistry 2020Quote: Recombinant bacmids containing the gene of interest (PDI) were generated according to the Baculovirus Expression system manual (Thermo Fisher Scientific, Cat. No. 10359016). In brief ...
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bioRxiv - Microbiology 2022Quote: Cu-sensitive strains harboring the recombinant pTR plasmids were harvested for metal content determination by using inductively coupled plasma mass spectrometry (ICP-MS) (Thermo Fisher Scientific, USA). E ...
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bioRxiv - Neuroscience 2020Quote: ... The same recombinant Cdk5/p25 protein used for the MST assays was labeled with biotin using EZ-LinkNHS-PEG4-Biotinylation Kit (Thermo Scientific, Waltham, MA) and excess reagent was removed with a spin desalting column as per the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... The linearized recombinant plasmid was used as templates to synthesize capped RNAs (cRNAs) with the mMessage mMachine T7 Kit (Life Technologies, Carlsbad, CA). The cRNAs were dissolved in RNase-free water at a concentration of 1000 ng/μL ...
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Preprotech, cat#100- 18B) and 50 ng/mL recombinant sonic hedgehog (SHH, Preprotech, cat#100-45) in Knockout DMEM (Gibco cat #10829-018), 10% Knockout serum replacement (KOSR ...
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bioRxiv - Microbiology 2019Quote: ... and then stained with anti-LCMV-WE recombinant NP immunized rabbit serum and HRP-goat anti-rabbit IgG (H+L) DS Grd (Life Technologies, Carlsbad, CA) (41) ...
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bioRxiv - Microbiology 2021Quote: ... ExpiSf9 cells for recombinant baculovirus production were cultured at 27°C and 125 rpm in ExpiSf CD Medium (Thermo Fisher Scientific, Waltham, MA). B19V-infected plasma sample was obtained from a donation center (CSL Behring AG ...
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bioRxiv - Biochemistry 2019Quote: ... A concentration range of indicated recombinant proteins was analyzed by thermal melt assays in SEC buffer using SYPRO Orange (Molecular Probes, cat.# S6651) using a Biorad CFX96 Touch Real-Time PCR C1000 System (ramping from 4 to 100°C at 1°C per minute).
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bioRxiv - Microbiology 2019Quote: ... and stained with anti-LCMV-WE recombinant NP immunized rabbit serum and HRP-goat anti-rabbit IgG (H+L) DS Grd (lot: 917439A, Life Technologies, Carlsbad, CA) (41) ...
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bioRxiv - Cell Biology 2021Quote: ... were delivered into H9 cells along with recombinant True Cut Cas9 v2 using the Neon Transfection system (Thermo Fisher Scientific, Cat. No MPK5000). H9 cells (1×105 cells ...
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bioRxiv - Microbiology 2020Quote: ... recombinant proteins were purified by affinity chromatography as described previously (27) and loaded on NuPAGE 4-12% BisTris gels (Invitrogen, Carlsbad, CA, USA) to confirm the correct molecular weights and assess their purity.
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bioRxiv - Microbiology 2022Quote: ... Samples were washed in blocking buffer thrice and stained with 1:1000 dilution of Alpaca anti-Rabbit IgG Nano (VHH) Recombinant Secondary Antibody conjugated with Alexa Fluor™ 647 (Invitrogen SA5-10327), Goat anti-Mouse IgG conjugated with Alexa Fluor™ Plus 488 (5 μg/mL final concentration ...
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bioRxiv - Neuroscience 2022Quote: ... The injections were either a fluorescent dextran amine or a recombinant cholera toxin subunit B tracer: (Dextran-405; CTB-488, CTB-594, CTB-647, Invitrogen #C22841, #C34777, #C34778). We chose our injection volume based on the size ...
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bioRxiv - Genomics 2022Quote: ... The mini prep DNA was then used with the Bac-to-Bac™ expression system to generate a recombinant bacmid as per manufacturer conditions with MAX efficiency™ DH10Bac™ Competent Cells (ThermoFisher). After 4 hours of transformation recovery ...
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bioRxiv - Microbiology 2023Quote: ADCP was measured using a flow cytometry-based phagocytosis assay.98 Biotinylated recombinant HIV-1 gp120 from strain CN54 (Acro Biosystems; catalog #GP0-V182E6) was combined with fluorescent NeutrAvidin beads (Life Technologies; catalog #F8776) at a concentration of 1 µg protein per µl bead and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 500 nM recombinant MEC-12/MEC-7 tubulins and a kinase candidate [500 nM recombinant MBK-2m or 475 nM CDK1/Cyclin B (Thermo Fisher; Cat# PV3292)] were incubated in MEM buffer [100 mM MES ...
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bioRxiv - Microbiology 2023Quote: ... the binding of HIV+ plasma was detected using a biotin-tagged dimeric recombinant soluble FcγRIIIa (0.2 μg/mL) followed by the addition of Alexa Fluor 647-conjugated streptavidin (Thermo Fisher Scientific; 2 μg/mL). Infected cells were then stained intracellularly for HIV-1 p24 ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: MARC-145 cells transfected with the recombinant pCAGGS-N plasmid were lysed in Pierce™ IP Lysis Buffer (Thermo Fisher Scientific, Waltham, MA), and then mixed with each of the purified anti-N mAbs ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrations of recombinant proteins were determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Cat#A53225, Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Biochemistry 2023Quote: ... The molecular weight of the recombinant proteins was estimated using Invitrogen™ BenchMark™ Pre-stained Protein Ladder (Fisher Scientific, Waltham, Massachusetts, USA). Eluted proteins were concentrated using Vivaspin® 10,000 MWCO centrifugal filter units (Sartorius ...
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bioRxiv - Cancer Biology 2022Quote: One million of BMDCs cultures in the absence or presence of 10 ng/ml of recombinant MDK (indicate provider and origin) were incubated with 1 μg/ml of Ovalbumin-AF594 conjugate (Thermo Fisher Scientific; O34781) for 30 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Purity and concentration of the recombinant proteins was assessed by SDS-PAGE and the Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Gel filtration was used to recover the recombinant protein fraction according to its expected size on an AKTA Ettan LC FPLC System (Amersham Pharmacy Biotech ...
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bioRxiv - Immunology 2024Quote: ... pH 7.34 for a short stimulation of 30 min) or in a CBMC medium supplemented with recombinant h-SCF (100 ng/mL, Gibco, PeproTech, #300-07) (for a longer stimulation) ...
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bioRxiv - Biochemistry 2021Quote: ... All RNA solutions were prepared by solubilizing in nuclease free water in the presence of RNAseOUT™ recombinant ribonuclease inhibitor (Thermo Fisher, Cat. #. 10777019) at a final concentration of 0.4 U/μL ...
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bioRxiv - Microbiology 2020Quote: ... the recombinant proteins were produced in their respective expression systems according to standard procedures and purified using either Ni-NTA (ThermoFisher Scientific, Waltham, MA, USA) or Strep-Tactin (IBA Lifesciences ...