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Citations for Thermo Fisher :
4601 - 4650 of 10000+ citations for 6 Chloro 9 3 N 2 chloroethyl ethylamino propylamino 2 methoxyacridine dihydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with Alexa-Flour-488 or 555 secondary antibodies (1:1000) along with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Camarillo, CA, 1:1000) for 1 hr at room temperature (RT) ...
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bioRxiv - Biochemistry 2020Quote: ... Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’, 6-diamidino-2-phenylindole) (Molecular Probes, Eugene, OR, US). Images were collected using an Axio Plan2/AxioCam microscope and image processing was performed with MrC5/Axiovision software (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... bacteria were stained with 2µg/µL of 4’,6-diamidino-2-phenylindole (DAPI) and mounted in ProLong Diamond Antifade (Life Technologies, Eugene, OR, USA; P36961). Image acquisition was performed using a Leica confocal microscope (Leica ...
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bioRxiv - Cancer Biology 2022Quote: ... To quantitate cell migration 6 fields of view per membrane were photographed with EVOS™ FL Auto 2 Imaging System(Thermo Scientific™ Invitrogen™), followed by quantification by ImageJ v1.53q (National Institutes of Health ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were stained with DAPI (4’,6-diamidino-2-phenylindole) and cover-slipped with ProLong Glass Antifade Mountant (P36984, Thermo Scientific, Waltham MA, USA). All images were acquired on a Keyence-BZ800 microscope ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... After several wash and centrifugations, nuclei were suspended in a solution (phosphate=buffered saline containing 1% 4’, 6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Eugene, OR, USA), and nuclei density was determined in a hemocytometer ...
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bioRxiv - Cell Biology 2021Quote: ... HCT8 cells were grown on a 6-well slide (3×105/well, Thermo Scientific) overnight ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells (3×105) were seeded in a 6 well plate (Nunc,Thermo,USA) with DMEM media (Invitrogen,USA ...
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bioRxiv - Cancer Biology 2019Quote: ... Spheroids pictures were taken after 3 and 6 days using EVOS FL microscope (Thermofisher) and the images were analyzed with ImageJ software ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Slices were finally incubated for 3-6 minutes with DAPI (1:10 000, Invitrogen) diluted in PBS and mounted on Polysine® Slides stored at 4°C until imaging.
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bioRxiv - Microbiology 2023Quote: ... Substrate 2,2’-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ABTS; Thermo Fisher Scientific) was added ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were blocked in 6% BSA and immunolabeled with anti-α3 antibody (mouse Anti-ATP1A3 xVIF 9-G10, MA3-915, Invitrogen, Thermo Fisher scientific) for 2 hours 200 times in PBS1x containing 1%BSA or at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... transfectants were selected with 3.0 μg/mL puromycin dihydrochloride (A1113803, Gibco) for 36h and then plated onto 6 cm dishes at clonal density ...
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bioRxiv - Microbiology 2022Quote: ... the cultures were supplemented with Raltegravir and Puromycin Dihydrochloride (ThermoFisher Scientific) was added at 2 µg/ml twice a week for 14 days to select transduced cells ...
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bioRxiv - Cell Biology 2021Quote: ... and with p-Phenylenediamine dihydrochloride (Thermo Fisher Scientific, 41 μg/ml) in PBS as an antifade ...
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bioRxiv - Neuroscience 2023Quote: ... a size exclusion layer of m-phenylenediamine dihydrochloride (Acros Organics, NJ) was electroplated to all four platinum recording sites with the use of the FAST16 mkIII system (Fast Analytical Sensor Technology Mark III ...
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bioRxiv - Cell Biology 2024Quote: ... cell selection was undertaken using 4 µg/mL puromycin dihydrochloride (Gibco). ELOVL5 upregulation was confirmed by Western blot as described previously27.
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bioRxiv - Cell Biology 2020Quote: ... and TagRFP-T-VAPB(1-218)-eMagB or TagRFP-T-eMagB-PHOSBP (prey) and iRFP-P4C at a 3:2:1 ratio in OptiMEM-I (Thermo Fisher Scientific) (1:4 DNA ...
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bioRxiv - Cell Biology 2020Quote: Total cellular mRNA was isolated from cells grown in 2-D and 3-D culture conditions using the GeneJET RNA purification kit (Thermo Fisher Scientific). For analysis of germ layer markers ...
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bioRxiv - Cell Biology 2020Quote: An aliquot of each sample equivalent to 3 μg was loaded onto a trap column (Acclaim PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (Acclaim PepMap RSLC column C18 2 μm ...
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bioRxiv - Biochemistry 2022Quote: ... The trap column used for the nano HPLC was a 2 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Thermo Fisher Scientific) and the separation column was a 12.5 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Nikkyo Technos Co. ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptide mixtures were loaded on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3.5 minutes at 5 μL/min with 2% Acetonitrile MS grade (Sigma Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... RNA interference was done by transfecting 100 nM siRNA (TPD54 1: GUCCUACCUGUUACGCAAU, TPD54 2:CUCACGUUUGUAGAUGAAA, TPD54 3:CAUGUUAGCCCAUCAGAAU) with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Peptides were trapped on a C18 reversed-phase trap column (2 cm x 75 μm ID; Acclaim PepMap trap column packed with 3 μm beads, Thermo Fisher Scientific) and separated on a 25 cm C18 reversed-phase analytical column (25 cm x 75 μm ID ...
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bioRxiv - Biochemistry 2019Quote: ... The peptides were loaded on a reversed-phase trap column (75 μm × 2 cm, 3 μm, C18, 100 Å, Thermo Scientific, Germany), and then the eluent was transferred to a reversed-phase analytical column (50 μm × 150 mm ...
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bioRxiv - Cancer Biology 2020Quote: ... were concentrated and desalted on a trapping pre-column (Acclaim PepMap C18, 75 µm × 2 cm nanoViper, 3 µm, 100 Å, Thermo Scientific), using 0.2% formic acid at a flow rate of 5 µl/min ...
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bioRxiv - Biochemistry 2020Quote: ... Peptide sample (2 μL) was injected onto an Acclaim PepMap 1000 trap column (75 μm × 2 cm, nanoViper C18 3 μm, 100Å, Thermo Fisher Scientific) which was heated to 40 °C in a chamber which was connected to a C18 reverse-phase Aurora UHPLC Emitter Column with nano Zero & Captive Spray Insert (75 μm × 25 cm ...
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bioRxiv - Biochemistry 2020Quote: ... N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine (PED-A1) and BODIPY™ FL C5 were purchased from Thermofisher scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed with PBS (3 x 10 min) and incubated 2 hours with the appropriate secondary antibodies (Invitrogen, CA, USA) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... washed 3 times with blocking buffer and incubated for 2 h with Alexa 647 anti-rabbit (1/250, A21247, Thermo Fisher Scientific). After washing the cells 3 times with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... approximately 1 pmol injected onto a Acclaim PepMap 100 guard column (75 μm×2 cm C18, 3 μm particles, 100 Å; Thermo Scientific) and separated on a 50 cm PepMap RSLC C18 (75 μm×50 cm ...
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bioRxiv - Neuroscience 2020Quote: Cells were electroporated with 2 μg of donor construct and 3 μg of px330-Cas9 plasmid using a Neon Transfection System (Thermo Fisher Scientific) and the following settings ...
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bioRxiv - Biophysics 2020Quote: ... We grew HL-60 cells to approximately 1 × 106 cells/ml and passaged the cells every 2-3 days in Roswell Park Memorial Institute medium (RPMI-1640, Thermo Fisher Scientific) supplemented with L-glutamine and 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... BV-2 immortalized microglial cells (Blasi et al. 1990) (passage 3 to 8) cultured in macrophage serum-free medium (ThermoFisher 12065-074) were used ...
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bioRxiv - Biochemistry 2022Quote: The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Orbitrap Exploris 480 for replicate 2, and Q-Exactive HF for replicate 3, Thermo Fisher Scientific). Peptides were sampled on a precolumn (300 μm × 5 mm PepMap C18 ...
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bioRxiv - Immunology 2022Quote: ... Peptide lysates were injected on a trapping column (Acclaim PepMap 100 C18, 3 μm, nanoViper, 75 μm x 2 cm, Thermo Fisher Scientific) with 5 μl/min ...
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bioRxiv - Biochemistry 2021Quote: ... spectra were recorded on a Thermo-Finnigan Surveyor equipped with a Satisfaction RP18AB C18 3 μm (15×2 mm) (Cluseau) and coupled to an ESI LCQ Advantage (Thermo Fisher Scientific). The running mixture was subjected to filtering on a Microcon filter unit of 10 kDa (15 min ...
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bioRxiv - Microbiology 2021Quote: ... using an Acclaim PepMap100 pre-column (C18, 3 μm-100 Å) and a PepMap RSLC (C18, 2 μm, 50 μm-15 cm) both from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... peptides were loaded onto a trap column (PepMap C18 trap column 75 µm × 2 cm, 3 µm, particle size, 100 Å pore size; ThermoFisher Scientific) at 5 μL/min for 3 min before switching the pre-column in line with the analytical column (PepMap C18 analytical column 75 μm × 50 cm ...
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bioRxiv - Systems Biology 2019Quote: ... equipped with a reverse phase trap column (Acclaim PepMap 100, 75 µm x 2 cm, 3 µm, 100 Å; Thermo Fisher Scientific) and 50 cm analytical column (EASY-Spray ...
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bioRxiv - Microbiology 2020Quote: ... incubating with primary antibodies (rabbit anti-β-actin, 1:1000, Thermo Fisher Scientific, PA1-183; mouse anti-claudin-2, 3:500, Invitrogen, 325600 ...
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bioRxiv - Cancer Biology 2020Quote: ... LC separation was executed with a trap column (C18, 75 μm*2 cm, 3 μm) (cat No. 164946, Thermo Fisher Scientific, Germany) and an analytical C18 column (C18 ...
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bioRxiv - Bioengineering 2019Quote: ... medium was switched to a B27-based Retinal differentiation medium (BRDM) (DMEM/F12 (3:1) with 2% B27 (w/o vitamin A, ThermoFisher Scientific, USA), 1x NEAA and 1x AA) ...
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bioRxiv - Microbiology 2020Quote: ... were performed on an Ultimate 3000 RSLC nano instrument coupled to either a QExactive Plus (M#1, M#3) or an HF mass spectrometer (M#2; Thermo Fisher Scientific) as described previously.24 Tryptic peptides were trapped for 4 min on an Acclaim Pep Map 100 column (2 cm × 75 μm ...
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bioRxiv - Immunology 2020Quote: ... D-PBS (D-PBS without Ca++ and Mg++) supplemented with 2% FBS and 3 mM cell culture grade EDTA (Life Technologies; Thermofisher) prior to monocyte isolation ...
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bioRxiv - Genomics 2021Quote: Human brain tissues were manually dissected into small 2-3 mm3 pieces and immersed in homogenization buffer (HBSS (Life Technologies, 14175-095), 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: N2A cells were transfected as described above with BicD2-KIF tail fusions and MBNL-GFP proteins at a ratio of 2:3 in 4-well chamber slides (Thermo Fisher, 154526). 24 hours after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were loaded onto a trap column (Dionex-C18 trap column 75 μm × 2 cm, 3 μm, particle size, 100 Å pore size; ThermoFisher Scientific) at 5 μl/min before switching the pre-column in line with the analytical column (Dionex-C18 analytical column 75 μm × 50 cm ...
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bioRxiv - Biochemistry 2022Quote: ... The trap column used for the nano HPLC was a 2 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Thermo Fisher Scientific), and the separation column was a 12.5 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Nikkyo Technos ...
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bioRxiv - Cell Biology 2022Quote: ... a Thermo Scientific EASY-nLC 1000 liquid chromatography system coupled with an Acclaim PepMap 100 (2 cm x 75 μm, 3 μm particles, Thermo Fisher Scientific) pre-column in line with an EASY-Spray PepMap RSLC C18 reversed phase column (50 cm x 75 μm ...