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Citations for Thermo Fisher :
4551 - 4600 of 10000+ citations for 6 Chloro 9 3 N 2 chloroethyl ethylamino propylamino 2 methoxyacridine dihydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... onto a nanotrap column (75 μm i.d. x 2 cm precolumn, packed with Acclaim PepMap100 C18, 3 μm, 100 Å; Thermo Fisher) using an EASY-nLC 1200 system (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.05 M TBS and then incubated in the dark for 2 h at room temperature with secondary antibodies: donkey anti-mouse Alexa Fluor 488 (3 µl/ml; Life Technologies) and donkey anti-rabbit Alexa Fluor 594 (5 µl/ml ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were aliquoted in 10 mL (OD600 ∼ 0.2) and re-grown for 2-3 h to extract plasmids using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) for sequencing and for subcloning into the expression strain ...
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bioRxiv - Microbiology 2024Quote: ... the samples were stained with 80 μL CellEvent Caspase 3/7 Green detection Reagent 2 µM in PBS with 5% FBS (Invitrogen, C10423) and then incubated for 30 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Microbiology 2020Quote: ... Israel) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Pathology 2020Quote: ... 6-dihydroxy-2,4,5,7-tetraio-dospiro (isobenzofuran-1(3H),9[9H] xanthan)-3:1 dipotassium salt (50 mg/kg in 0.9% saline, Fisher Scientific) was injected retro-orbitally before catalyzing vessel injury with a 540 nm laser ...
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bioRxiv - Immunology 2020Quote: ... TIB-47) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Synthetic Biology 2020Quote: ... transfection complexes containing 3 μL of Xtremegene-9 reagent and 500 ng of plasmid DNA were prepared in 100 μL of OptiMEM (Invitrogen). After 20 min of incubation at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... A 11.2 kb PCR product that included a region from CLN3 exon 3 to exon 9 was amplified using a high fidelity polymerase (Platinum Taq High Fidelity, Invitrogen) and CLN3 primers pCLN3F3 and pCLN3R3 (Supplemental Table 2) ...
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bioRxiv - Microbiology 2024Quote: ... Israel) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was assessed at different time points (3, 5, 7 and 9 days) using AlamarBlue Cell Viability Reagent (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... N,N,N’,N’- tetramethyl ethylenediamine and glycylglycine were purchased from Acros Organics (New Jersey, USA). Protogel 30% acrylamide ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ng/mL (n = 3) and 10 ng/mL (n = 3) treatment were subjected to albumin depletion according to the manufacturer’s instructions (Thermo Fisher Scientific, Loughborough, UK). Individual samples were digested with trypsin (2.5µg trypsin ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Samples of the visceral yolk sac (n=6/group) were immersed in RNA Later solution (Invitrogen, MA, USA) and frozen at −20°C until further processing ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Bioengineering 2021Quote: ... N,N′-Diisopropylcarbodiimide (DIC, Fisher Scientific) and 10 equivalents of DIPEA in DMF for 4 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were then washed with PBT for 2 h and stored in 2 % DABCO (Acros Organics, AC112471000).
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bioRxiv - Neuroscience 2021Quote: The cultured cells were incubated with Fura-2-acetoxymethyl ester (Fura-2-AM; Molecular Probes; 2.5 µM) in MEM (0.7 mL ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from the SARS-CoV-2 infected Caco-2 cells using Trizol (Thermo Fisher). The extracted RNA was then reverse transcribed with the reverse primers specific to Nsp12 ...
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bioRxiv - Microbiology 2021Quote: ... LNPs were stained with 2% uranyl acetate for 2 mins and imaged by Talos F200X (Thermo Fisher) at an accelerating voltage of 200 kV ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL malt extract (2% w/w) in 4 mL screw neck vials (Fisher Scientific, Loughborough, UK) were inoculated with an agar piece of the G ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 µl cDNA was combined with 25 µl 2× TaqMan Gene Expression Master Mix (Thermo Fisher Scientific), 2.5 µl of 20× TaqMan Gene Expression Assay (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Lipofections were performed using 2 μg gRNA plasmid and 2 μg donor construct in OptiMEM medium (GIBCO) and Lipofectamin Stem reagent (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... Purified PCR products were sequenced and analyzed with the Sequence Scanner Software 2 Version 2 (Applied Biosystems).
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bioRxiv - Immunology 2020Quote: ACE-2 protein (hsACE-2, residues S19 to D615) was transiently transfected into HEK293E cells (Thermo Fisher) with PEI MAX (Polysciences ...
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bioRxiv - Biochemistry 2022Quote: ... The peptide sample was loaded onto the precolumn (75 μm×2 cm, C18, 2 μm, Thermo Fisher) at a flow rate of 400 nL/min and then separated using a reversed-phase analysis column (50 μm×15 cm ...
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bioRxiv - Cell Biology 2022Quote: ... in HBSS (Hanks Balance Salt Solution) without Ca+2 and Mg+2 (Thermo Fisher Scientific, Waltham, MA.) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Transferences were done in an iBlot 2 gel transfer device using iBlot 2 transfer nitrocellulose stacks (Invitrogen). Membranes were blotted against phospho-AKT (Ser473 ...
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bioRxiv - Cancer Biology 2024Quote: ... packaging plasmid psPAX2 and pMD2G in a ratio of 2:2:1 using lipofectamine 3000 (ThermoFisher, L3000001) and transduced into NES cells and ONS76 cells ...
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bioRxiv - Genomics 2024Quote: ... each with 2 µg PCR input and 2 µL FastDigest® LguI (Thermo Fisher Scientific, Waltham, MA). These reactions were then incubated at 37°C for two hours ...
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bioRxiv - Cell Biology 2024Quote: ... adipose tissue (2∼4 cm3) was washed twice with PBS solution (AQ) containing 2% penicillin-streptomycin (Invitrogen), and then cut to 1∼2 mm3 with scissors ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 of the remaining cells were stimulated with 2×106 anti-CD3/CD28 beads (Gibco, 11453D) in 5 ml of IMDM medium supplemented with 10 % FBS ...
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bioRxiv - Microbiology 2023Quote: ... 2′–5′ OA and dephosphorylated OH-2′–5′ OA were transfected into cells using Lipofectamine 2000 (Invitrogen) according to the protocol provided by the manufacturer.
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bioRxiv - Microbiology 2023Quote: 2 x104 MPI-2 cells/well were seeded in a white clear-bottom 96 well plates (ThermoFisher) and incubated at 37°C with 5% CO2 for 24 hours ...
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bioRxiv - Microbiology 2023Quote: 2 x104 MPI-2 cells/well were seeded in a flat clear-bottom 96 well plates (ThermoFisher) and incubated at 37°C with 5% CO2 for 24 hours before the treatment with 15 nmol of either sfGFP or LysBFL ...
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bioRxiv - Cancer Biology 2024Quote: ... OPM-2 cells were labelled with 2 μg/mL of Vybrant DiD cell-labeling solution (Life Technologies). The labeled cells were suspended in RPMI containing 2 mM EDTA ...
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bioRxiv - Cell Biology 2019Quote: ... with NuPAGE 3-(N-morpholino) propane sulfonic acid (MOPS) sodium dodecyl sulfate (SDS) running buffer (Invitrogen). Proteins were then transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Bioengineering 2019Quote: ... n=3 pellets/condition/cell-type) and cultured in differentiation media (StemPro Chondrogenic Media; ThermoFisher Scientific) for 28 days ...
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bioRxiv - Cancer Biology 2020Quote: ... cell viability (n =3) was assayed at different times using the PrestoBlue cytotoxicity assay (Thermo Fisher), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Laser Capture Microdissection (LCM; N=3; 7 µM glass slide coated with polyethylene naphthalate – ThermoFisher #LCM0522), multiplex or regular immunohistochemistry (N≥3 4 µM glass slide ...
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bioRxiv - Neuroscience 2022Quote: Tissue cultures (n=3) were transferred as one sample into 250 μl RNAlater (ThermoFisher, Cat# AM7020) and stored at −20 °C ...
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bioRxiv - Neuroscience 2023Quote: Retinal tissues (N = 3 biological replicates) were lysed with RIPA buffer (Thermo Fisher Scientific, Cat.# 89900) containing protease and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... 150 mM NaCl) containing N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM; Invitrogen) at a final concentration of 10 μM and incubated for 30 min in the dark on ice ...
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bioRxiv - Cell Biology 2023Quote: ... 9 µL of the reaction was stopped with 9 µL NuPAGE LDS Sample Buffer (Invitrogen) at 0 ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted and quantified from WwoxWT/WT and WwoxP47T/P47T n=6 mice/group (3 males and 3 females) and cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Cancer Biology 2020Quote: ... MiRNAs or vectors were transfected in the amount of 2 μg for 6-well plate into indicated cells by Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... the coverslips were placed upside down on a glass slide with slow fade mounting medium containing 4’,6-diamidino-2- phenylindole (DAPI, Life Technologies, Carlsbad, CA, USA) and kept at 4 °C until observation ...
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bioRxiv - Microbiology 2020Quote: ... were acid digested on a hot plate using 6 mL Primar Plus grade HNO3 (68%) and 2 mL H2O2 (Thermo Fisher Scientific, Loughborough, UK). Samples were diluted with Milli-Q water (18.2 MΩ cm ...