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Citations for Thermo Fisher :
4551 - 4600 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... intestinal fragments were incubated in epithelial cell solution (10 mM EDTA [Invitrogen, #15575020]-HBSS, 5 mM HEPES [Invitrogen, #15630080], 100 U/ml Pen-Strep [Invitrogen, #15140122] ...
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bioRxiv - Neuroscience 2023Quote: ... iPS-MNs were resuspended in NDM medium (Neurobasal Medium supplemented with Glutamax, 0.1mM 2-mercaptoethanol, N2, Pen/Strep, MEM NEAA, ThermoFisher, 11140050), supplemented with GDNF (10 µg/ml) ...
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bioRxiv - Developmental Biology 2023Quote: ... Finally single cells were sorted into a gelatin coated 96well plate containing Serum medium supplemented with Pen/Strep (Catalog no. 15140122, Gibco). Based on fluorescence and morphology ...
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bioRxiv - Developmental Biology 2023Quote: ... coated plates in growth media (DMEM with 20% FBS, 5% horse serum, 1% pen-strep, 1% glutamax (above from Gibco), 0.1% chick embryo extract (MPbio ...
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bioRxiv - Bioengineering 2023Quote: ... 10 µL of the grown pre-culture was re-inoculated to MTP-R48-B FlowerPlate (m2p-labs) containing 1 mL minimal medium + Pen/Strep (Invitrogen). The plate was incubated 48 h in Biolector® at 30°C ...
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bioRxiv - Bioengineering 2024Quote: ... at a density of 300,000 cells per flask using sterile filtered α-MEM media supplemented with 10% FBS and 1% Pen-Strep (Gibco), referred to as α-MEM culture media ...
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bioRxiv - Physiology 2024Quote: ... were cultured in pen/strep-free media overnight and transfected with 25 nmol/L of either Lgr4 (Thermo Fisher Scientific) or scrambled (SC ...
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bioRxiv - Immunology 2024Quote: RMA/S cells were cultured in media (Gibco RPMI Medium 1640 containing 10% Gibco FBS and 1% Gibco Pen Strep) at 37°C ...
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bioRxiv - Immunology 2024Quote: RMA/S cells were cultured in media (Gibco RPMI Medium 1640 containing 10% Gibco FBS and 1% Gibco Pen Strep) at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... using a pDSG-IBA-Twin-Strep-Tag vector as template (IBA-lifesciences) into the HindIII-ApaI site of pcDNA5-FRT-TO (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... 1.65×106 FL3 cells were seeded in a P10 dish and transfected 24 hrs later with 12 µg total DNA (2 μg Twin-Strep-Tag-RBP expression vector and 10 µg pcDNA3 PL) using Lipofectamine 2000 (Invitrogen) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... Rescue cell lines with stable expression of N-terminally-tagged Flag- or Strep-TTC5 (WT and mutants) were generated from the TTC5 knockout cells using the Flp-In system (K650001 Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The Madin-Darby Canine Kidney II (MDCK-II) cell line was maintained in Eagle’s Minimum Essential Medium (EMEM, Gibco), supplemented with 5% heat-inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... Gateway recombination reactions were performed with BP Clonase II and LR clonase II enzyme mixes (Invitrogen, Carlsbad, CA, USA) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were then rinsed and stained with secondary antibody (biotinylated horse anti-mouse; Gibco; BA2000; 1:2000) for 1h at room temperature before rinsing and incubation with streptavidin-HRP (SNN1004 ...
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bioRxiv - Immunology 2021Quote: ... These antibodies were biotinylated using an EZ-Link micro Sulfo-NHS-biotinylation kit (ThermoFisher Scientific, Waltham, MA) before the incubation ...
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bioRxiv - Immunology 2022Quote: BG505 SOSIP trimers were randomly biotinylated using the EZ-Link NHS-PEG4-Biotin kit (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2022Quote: ... wells were then incubated for 1 hour at room temperature with biotinylated antibody against IL-10 (Invitrogen) and then incubated with HRP-conjugated streptavidin (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then biotinylated using NHS-PEG4-Biotin (125 µM unless otherwise specified, ThermoFisher Cat. No. A39259) diluted in PBSCMA to achieve a total volume of 50 µL per well for 30 minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Up to 150 ng of the DNA was then biotinylated with Streptavidin C-1 beads (ThermoFisher Scientific). Tagmentation was performed using Nextera DNA Library Preparation Kit (#FC-121-1030 ...
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bioRxiv - Neuroscience 2020Quote: ... was added to each sample and mRNA was captured using modified oligo-dT biotinylated beads (Dynabeads, Invitrogen). PCR amplification was performed for 22 cycles ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 pmol Biotinylated RNA oligos were conjugated with 50 μl of streptavidin beads (50% slurry; Thermo Fisher) in a total volume of 300 μl of RNA-binding buffer (20mM Tris ...
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bioRxiv - Bioengineering 2020Quote: ... Antibodies were biotinylated using an EZ-Link™ micro Sulfo-NHS-biotinylation kit (ThermoFisher Scientific, Waltham, MA). For surface marker EV detection ...
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bioRxiv - Bioengineering 2021Quote: ... The absorbance spectrum of UV-cleavable biotinylated cDNA-antibody conjugates were measured by a Nanodrop spectrophotometer (Thermofisher) to determine the degree of labeling (DOL ...
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bioRxiv - Biophysics 2021Quote: ... the respective ligands were biotinylated using the biotinylation kit EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). The biotin label was resolved in H2O and directly added to the protein solution in a final molar ratio of 1:1 in 25 mM MES ...
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bioRxiv - Microbiology 2021Quote: ... the RBD was biotinylated using EZ-Link NHS-LC-LC-Biotin (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Immunology 2021Quote: ... biotinylated SARS-2 and Rs4081 RBDs were incubated with streptavidin-APC (eBioscience™) and streptavidin-PE (ThermoFisher), respectively ...
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bioRxiv - Cell Biology 2021Quote: To prepare biotinylated salmon sperm DNA for DNA-carpets 10 mg/ml salmon sperm DNA (Thermo Fisher) was sonicated for 5 min (110 watts ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 790 ng of the products were biotinylated with Pierce RNA 3’ End Biotinylation Kit (Thermo Fisher Scientific). After ligation of 15 h ...
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bioRxiv - Microbiology 2020Quote: ... RBD-6H (340-538; NITN.GPKK) was chemically biotinylated using EZ-link Sulfo-NHS-Biotin (A39256; Life Technologies). Serial half-log dilutions (starting at 1 μM ...
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bioRxiv - Genomics 2019Quote: ... and the 5’ overhang was repaired using a biotinylated residue (0.4 mM biotin-14-Datp (INVITROGEN, America). The resulting blunt-end fragments were ligated in situ (10X NEB T4 DNA ligase buffer (NEB ...
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bioRxiv - Molecular Biology 2019Quote: EMSA was performed using double stranded biotinylated DNA probes and the Lightshift Chemiluminescent EMSA kit (Thermo Scientific). Biotin 3’ end-labeled DNA oligomers were prepared using a biotin end-labeling kit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... or PD41 underwent stereotaxic surgery to inject biotinylated dextran amine (BDA; 10,000MW anterograde neural tracer; Life Technologies NeuroTrace BDA 10000 Neuronal Tracer Kit ...
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bioRxiv - Genomics 2019Quote: ... Biotinylated primers and undigested inserts were removed as before using Dynabeads M-270 Streptavidin (Thermo Fisher Scientific). Ligation of pJDrcEPP_lib and minP-Luc2 inserts was performed at a 1:3 ratio as before but with T7 DNA ligase (New England Biolabs) ...
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bioRxiv - Microbiology 2019Quote: ... Anti-NS1 antibody binding was detected by a biotinylated goat anti-mouse IgG secondary antibody solution (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... The following secondary antibodies were used: biotinylated donkey anti-goat IgG (H+L) (Molecular Probes, Paisley, UK) and biotinylated donkey anti-rat IgG (H+L ...
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bioRxiv - Biophysics 2020Quote: ... The respective ligands were biotinylated using the biotinylation kit EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). The biotin label was freshly resolved in H2O ...
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bioRxiv - Neuroscience 2019Quote: ... we injected the axon tracer biotinylated dextran amine (BDA, 10,000 MW, 10% solution in water, Invitrogen, D1956) into the sensorimotor cortex 2 weeks before the mice were sacrificed ...
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bioRxiv - Neuroscience 2019Quote: ... A hybridization cocktail containing the biotinylated target was incubated with the GeneChip Mouse 2.0 ST array (Affymetrix) at 60rpm for 16hr at 45°C in a Genechip Hybridisation Oven 645 ...
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bioRxiv - Microbiology 2020Quote: ... the RBD was biotinylated using EZ-Link NHS-LC-LC-Biotin (Thermo Fisher Scientific, Waltham, MA, USA). The assay was carried out by placing the Streptavidin Biosensors (ForteBio ...
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bioRxiv - Immunology 2021Quote: ... fragments containing a biotinylated C region were captured by M270 beads which were crosslinked to streptavidin (Invitrogen), with a dissociation ...
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bioRxiv - Immunology 2019Quote: ... Clone 31-90-25 was biotinylated with the EZ-Link Micro Sulfo-NHS-Biotinylation Kit (Pierce, ThermoFisher). Samples were detected using streptavidin-HRP (Fitzgerald ...
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bioRxiv - Neuroscience 2019Quote: ... biotinylated dextran amine (BDA, 10 kDa; 10% solution dissolved in PB; Thermo Fisher Scientific, Waltham, MA, USA) was injected using either a brief air pulse (10–20 psi for 40–100 ms ...
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bioRxiv - Biochemistry 2021Quote: ... biotinylated Ube2k-UBC∼Ub was immobilised to Nunc Maxisorp 96-well plates (Fisher Scientific, Nepean, ON, Canada) coated alternately with neutravidin (New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: EVs were biotinylated using EZ-Link Sulfo-NHS-LC LC-Biotin (Thermo Fisher Scientific, Waltham, MA, USA). Biotinylated EVs were loaded onto Zeba spin desalting columns (7 K MWCO ...
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bioRxiv - Molecular Biology 2021Quote: ... Where required antibodies were biotinylated using EZ-link NHS-LC-Biotin according to the manufacturer’s instructions (Thermofisher), or were attached to cyanogen bromide-activated agarose using the manufacturer’s protocol (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... The trimeric spike SARS-CoV-2 and its variants were biotinylated (EZ-Link Sulfo-NHS-Biotin, ThermoFisher), desalted (Zeba Spike Desalting ...
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bioRxiv - Immunology 2022Quote: ... Bovine serum albumin (BSA) was biotinylated using the EZ-Link Micro NHS-PEG4-Biotinylation Kit (Thermo Fisher); excess unreacted biotin was removed using 7-kDa Zeba desalting columns (Pierce).
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bioRxiv - Microbiology 2022Quote: ... Biotinylated lectins were precomplexed (on ice, 20 min) with 0.2 µg streptavidin Alexa Fluor 488 (S32354, Invitrogen). For titration experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Then 0.1 μl of 10% biotinylated dextran amine (BDA, 10,000 MW, ThermoFisher Scientific, Waltham, MA, United States) or 4% FluroGold (FG ...