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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... A549 and PC9 cells were maintained in RPMI-1640 media supplemented with 10% Tet-Free FBS (Gemini) and 1x penicillin/streptomycin (pen/strep, Gibco) and HEK239T cells were maintained in DMEM media supplemented with 10% FBS (Gemini ...
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bioRxiv - Cell Biology 2019Quote: ... 10 ml of resuspension media (DMEM/F12 with glutamax supplemented with 15% FBS and 1% pen/strep; Thermo Scientific, CA) was added to digested solution and slowly inverted 5 times ...
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bioRxiv - Cancer Biology 2020Quote: 10U/ml penicillin G sodium and 10 mg/ml streptomycin sulfate (pen-strep; Gibco/ Invitrogen, Grand Island, NY; 15140-122).
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS, Seradigm) and 1% (v/v) penicillin and streptomycin (Pen-Strep, Gibco) (i.e. ...
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bioRxiv - Cell Biology 2021Quote: T47D cells were seeded in six-well plates (50 000 cells/well) in 2 ml of cell medium (10% hiFBS [Fisher] and 1% Pen/Strep [Invitrogen]). On Day 1 ...
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bioRxiv - Neuroscience 2019Quote: ... and resuspended in Neurobasal complete medium (NBC) (0.02% 10X SM1, 0.01% 100X Pen/Strep, 0.0025% glutamax in NeuroBasal Media (ThermoFisher Gibco 21103-049)) ...
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bioRxiv - Biochemistry 2020Quote: For the expression in insect cells ctCDK7 was first inserted into the pETtwin vector by SLIC and the resulting Twin-Strep-tag-3C-ctCDK7 construct was then transferred into a pFastBac1 transfer vector (Invitrogen) by restriction cloning using EcoRI and XbaI ...
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bioRxiv - Neuroscience 2020Quote: ... CLU199 cells were routinely maintained in DMEM culture medium supplemented with 10% FBS and 1% Pen-Strep (all from Gibco) and seeded on PLL-coated glass coverslips as needed ...
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bioRxiv - Cancer Biology 2021Quote: ... were cultured in EBM-2 Basal Medium supplemented with EGM-2 MV Microvascular Endothelial Cell Growth Medium and 1% v/v Pen-Strep (ThermoFisher) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... with 10% heat-inactivated fetal bovine serum (FBS; Gemini Bio, Sacramento, CA, USA, #GEMZR039) containing 1% penicillin and streptomycin (pen/strep) (Gibco; Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... RPE-1 cells were cultured in DMEM + GlutaMAXTM-I medium supplemented with 10% Fetal Bovine Serum and Pen strep (Gibco). RM 13 KO cell line was generated by pre-designed zinc finger nucleases (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... The biotinylation of the pT270 and noTel fragments was verified using AFM imaging of DNA samples in the presence of streptavidin-coated quantum dots (strep-QDs, Invitrogen). All oligos were purchased from IDT ...
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bioRxiv - Biochemistry 2022Quote: ... with a C-terminal Strep tag for purification and a foldon tag for trimerization was inserted into the pFastBac-Dual vector (Invitrogen) and was expressed using Bac-to-Bac baculovirus system (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... with a C-terminal Strep tag for purification and a foldon tag for trimerization was expressed in FreeStyle 293-F cells (Invitrogen). The plasmid was transiently transfected at a density of 2 × 106 cell per ml using polyethyleneimine (PEI ...
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bioRxiv - Cell Biology 2021Quote: ... The lentivirus-transduced HEK293T and hTERT-RPE1 cells to express mouse CatSpertS:WT-EGFP and CatSperτS:Mut-EGFP were passaged one time and cultured in the FBS- and Pen/Strep-containing media supplemented with and 50 μg/ml (HEK293T cells) or 20 μg/ml (hTERT-RPE1) hygromycin B (Invitrogen). Stable protein expression was checked by epifluorescence microscope (ZOE Fluorescent Cell Imager ...
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bioRxiv - Neuroscience 2021Quote: ... to 50 mL of Neurobasal medium (NeuroBasal medium (21203-049) with an added 1 % GlutaMAX (35050-061) and 1 % Pen-Strep (15070-063, all from ThermoFisher).
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 10% Fetal Bovine Serum (FBS; BioConcept Ltd. Amimed) and 1% Penicillin/Streptomycin (Pen/Strep; 15140122 Gibco, ThermoFisher Scientific), and maintained at 37 °C and 5% CO2.
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bioRxiv - Microbiology 2022Quote: ... At PPSC, HepG2 cells (ATCC, HB-8065) were cultured using DMEM supplemented with 0.1% Pen-Strep solution (Invitrogen; 15070-063) and 10% FBS (Gibco ...
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bioRxiv - Immunology 2022Quote: ... followed by an enterokinase cleavage site and a C-terminal double strep-tag was cloned into a modified pMT/BiP expression vector (pT350, Invitrogen). Drosophila S2 cells were stably co-transfected with pT350 and pCoPuro (for puromycin selection ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 10% Fetal Bovine Serum (FBS; BioConcept Ltd. Amimed) and 1% Penicillin/Streptomycin (Pen/Strep; 15140122 Gibco, ThermoFisher Scientific), and maintained at 37 °C and 5% CO2.
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bioRxiv - Developmental Biology 2019Quote: ... After 1 h ROCK-inhibitor was removed and was replaced with standard growth media supplemented with Pen-Strep (Life Technologies). Cells were stimulated with the following ligands or small molecules 12 h after seeding ...
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bioRxiv - Developmental Biology 2019Quote: HEK293 cells were passaged in HyClone DMEM (GE) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco) at 37°C and 5% CO2 under humidified conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... exchanging half of the media with Serum-Free medium (DMEM w/o Phenol Red-GIBCO-, 25mM HEPES, L-Glu, Pen/Strep, 1% glucose, B-27 and N2 supplement-GIBCO-) every 2 days ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with PBS and cultured for 4 days in basic differentiation medium (Advanced RPMI 1640, 1x GlutaMAX, Pen/Strep; all Gibco) supplemented with 8 µM CHIR99021 (Tocris ...
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bioRxiv - Immunology 2021Quote: ... cells were re-suspended in PBS/1% (v/v) FBS/1% (v/v) Pen/Strep/5mM EDTA/25mM HEPES (ThermoFisher).
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bioRxiv - Cancer Biology 2021Quote: ... and a combination of 100 U/ml Penicillin and 100 μg/ml Streptomycin (Gibco® Pen Strep, Thermo Fisher Scientific). The enzalutamide resistant LNCaP RES-C cell line was generated by passaging of LNCaP cells with continuous treatment with 10 µM enzalutamide for 9 months and maintained in the same medium as LNCaP except for the supplementation with 10 µM enzalutamide.
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bioRxiv - Cell Biology 2020Quote: ... with 10% FBS and 1% pen/strep and MCF10A cells were cultured in DMEM/F-12 supplemented with 5% horse serum (Gibco), 20ng/ml epidermal growth factor ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10 % foetal calf serum (FCS, Labtech) and 100 U/ml penicillin and 100 μg/ml streptomycin (Pen/Strep; Gibco). THP-1 cells were maintained in RPMI (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... and 1% v/v Pen/Strep (100 U/ml of penicillin, 100 μg/ml of streptomycin, Gibco, Cat#: 15140-122). A549 cells (Cat# ...
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bioRxiv - Immunology 2021Quote: ... were cultured in DMEM (ATCC) supplemented with 10% fetal bovine serum (FBS, Atlanta Biologics) and penicillin-streptomycin (Pen/Strep, Gibco).
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10 % foetal bovine serum (FBS, Labtech) and 100 U/ml penicillin plus 100 µg/ml streptomycin (Pen/Strep; Gibco). THP-1 cells were maintained in RPMI (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 CAR and NT-T cells CFSE-labeled were incubated with or without 1×106 OVCAR4 and Caov-3 cells for 48 h in RPMI-10% FBS-Pen/Strep and L-Glut medium in 10 cm cell culture dishes (Nunc). CFSE dilution was analyzed using flow cytometry on CD3+-gated cells ...
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bioRxiv - Cancer Biology 2022Quote: ... The remaining tissue was washed vigorously with cold DPBS containing Pen/Strep and Gentamicin for 10 times before incubating with 2.5 mM EDTA (Invitrogen, AM9260G) for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% Fetal Bovine Serum (EuroBio) and 1% Pen/Strep + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids solution (Gibco) at 37°C in 5% CO2 humidified incubators ...
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bioRxiv - Microbiology 2023Quote: Normal adult donor blood (in EDTA) was diluted 1:5 with RPMI 1640 supplemented with pen/strep and 2mM L-glutamine (Gibco). Cells were incubated at 37°C for 2 hours in the presence of polymyxin B (1.25μg/ml ...
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bioRxiv - Molecular Biology 2022Quote: Cells obtained after enzymatic digestion of testis were resuspended in 5-10 ml FACS buffer (1x DPBS [Gibco] supplemented with 1% Pen-Strep, 1% FBS, 10 mm HEPES, 1 mm pyruvate [Gibco] and 1 mg/ml glucose [Gibco]) and counted with a hemocytometer ...
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bioRxiv - Biochemistry 2024Quote: ... Unlabeled KBM7 cells were maintained in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% (v/v) FBS and 1x Pen-Strep (Gibco #15140-122). Unlabeled Jurkat cells were maintained in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Neuroscience 2024Quote: ... cortices from P3-P5 pups were micro-dissected into chilled Hibernate A (HibA) media (Hibernate A[Gibco], 1% Pen/Strep [Gibco]). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... and differentiated using 2.5% FBS neurobasal media (1X B27, 20 mM KCl, 1% Pen-Strep, 2 mM Glutamax (Thermo Fisher) 50 ng/mL Brain-derived neurotrophic factor (BDNF) ...
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bioRxiv - Developmental Biology 2023Quote: ... coated plates in growth media (DMEM with 20% FBS, 5% horse serum, 1% pen-strep, 1% glutamax (above from Gibco), 0.1% chick embryo extract (MPbio ...
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bioRxiv - Developmental Biology 2023Quote: ... Finally single cells were sorted into a gelatin coated 96well plate containing Serum medium supplemented with Pen/Strep (Catalog no. 15140122, Gibco). Based on fluorescence and morphology ...
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bioRxiv - Synthetic Biology 2022Quote: ... 35 μl of IBA MagStrep “type3” XT Strep-Tactin beads were equilibrated in a wash buffer of tris-buffered saline solution with 0.05% Tween 20 (ThermoFisher 28360) by washing twice with 35 ul of the wash buffer ...
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bioRxiv - Microbiology 2022Quote: ... and primary chicken embryo fibroblast cells (CEF) were cultured in DMEM containing 10% FBS,1% penicillin-streptomycin (Pen-Strep) (Gibco). Preparation of TKO cells has been previously described(Zhang et al. ...
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bioRxiv - Microbiology 2022Quote: ... sE genes with a tandem C-terminal strep-tag in pMT/BIP/V5 plasmid were expressed in Drosophila S2 cells (Invitrogen) as described previously (20) ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... Media were supplemented with alternating antibiotics after MACS to reduce the risk of bacterial contamination (50 μg/ml Kanamycin, 25 μg/ml chloramphenicol or 1x Pen/Strep, Gibco). Transformation was carried out by electroporation for libraries48 and using the Frozen-EZ Yeast Transformation II Kit (Zymo ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10 % foetal bovine serum (FBS, Labtech) and 100 U/ml penicillin plus 100 μg/ml streptomycin (Pen/Strep; Gibco). THP-1 cells were maintained in RPMI (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were plated in 6 well plates at 8 x 10^5 cells / well with DMEM / 10% FBS / NEAA / Pen-Strep (Gibco). Cells growing at ∼70-80% confluency were transfected with 1 μg of the desired expression plasmid (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed and blocked with 200 μl of complete RPMI 1640 (10% FBS, 1% Penn-Strep, 1% L-glutamine; Gibco) medium ...
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bioRxiv - Neuroscience 2023Quote: ... and re-plated on Matrigel-coated dishes in NPC Medium (DMEM/F12, 2% B27, 1% N2, 1% Pen-strep; all from Gibco) supplemented with 20 ng/mL bFGF (Peprotech) ...
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bioRxiv - Genomics 2023Quote: ... at Passage 184 was purchased from ATCC and grown under standard conditions (EMEM medium [ATCC, catalog # 30-2003] + 10% FBS [Gibco 26140-079], and Pen-Strep [Gibco 15140122] in an incubator set at 37°C with 5% CO2).