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Citations for Thermo Fisher :
4551 - 4600 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich], 1% bovine gelatin [Sigma-Aldrich], and 0.05% Tween 20 [Thermo Fisher Scientific]) was added and plates incubated for 2 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with a cocktail of secondary antibodies (1:500, Alexa Fluor 488, 546, 647) in blocking solution containing Hoechst 33342 (1:10,000, Life Technologies, Cat. No. H3570) overnight at room temperature followed by three final washes with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again re-blocked with 5% NGS for 2 hr (second serum blocking) and subsequently stained with rat anti-GFAP (1:500; Invitrogen Cat. #13-0300), and rabbit anti-Megf10 (1 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed several times with PBS and subsequently incubated with secondary antibody with the following antibodies in blocking solution for 30-60 minutes: donkey anti-rabbit Alexa Fluor 488 (Themo Fisher Scientific; A-21206), donkey anti-mouse Alexa Fluor 555 (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... Fixed cells were then washed in the blocking solution (0.3% BSA (Fisher Bioreagents, Cat# BP1600-100) and 0.01% Tween (Thermo Scientific, Cat# 9005-64-5) in 1x PBS) ...
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bioRxiv - Neuroscience 2023Quote: ... MSD GOLD 96-well small-spot streptavidin-coated microtiter plates (Meso Scale Discovery, Rockville, MD) were treated with a blocking step of 1% casein-based PBS (Thermo Scientific, Waltham, MA) for approximately 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-N-WASP (Cell Signalling #4848S; 1:100) followed by Alexa Fluor 568 conjugated secondary antibodies (Invitrogen; 1:1000 in blocking buffer). Actin tails were labeled with Alexa Fluor 488 phalloidin (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... incubated at room temperature for one hour with Alexa Fluor 647-conjugated donkey anti-mouse IgG diluted in blocking buffer (2 μg/ml, Molecular Probes, Cat #A31571) followed by washes in PBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... OCT-embedded penis sections were permeabilized in PBS with 0.3% Triton X-100 at room temperature for 10 min following by 1h incubation with blocking buffer containing 10% normal goat serum (Life Technologies, 16210-072) and 0.1% Tween20 in PBS at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were first incubated in blocking solution (2% bovine serum albumin [Sigma-Aldrich] and 0.3% triton X-100 [ThermoFisher] in 1X PBS) for 45 min at constant agitation and 4°C ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 488 (FLAG) and 594 (LCMV NP) Alexa Fluor antibodies (Life Technologies; 1:500 in blocking buffer), then washed four times with PBS.
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rat anti-Tbr2 (1:500, Invitrogen 14-4875-80), rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then transferred to new wells containing blocking solution with primary antibodies mouse anti PSD-95 (Thermo Fisher Scientific Cat. #MA1-045) at 1:250 ...
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bioRxiv - Cancer Biology 2023Quote: ... specific sites were blocked with a blocking buffer solution (5% fetal bovine serum [FBS, Life Technologies, #10270-098], 0.1% Tween 20 [Biosolve, #20452335] in PBS [Life Technologies, #14200-067]) for 30 min at RT and washed twice ...
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bioRxiv - Cell Biology 2023Quote: ... The protein-bound PVDF membranes were blocked with blocking solution (5% (w/v) non-fat dried milk in 1X PBS Tween™-20 (PBST) (ThermoFisher Scientific, 28352)) for 1 hour at room temperature (RT ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min each time and subsequently incubated with blocking buffer (5% goat serum [R&D Systems, Minneapolis, MN, Catalog no. S13110], 1% bovine serum albumin [Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then washed 2x with Blocking Buffer and subsequently incubated with Alexa Fluor 633 goat anti-mouse IgG (Invitrogen, A21050, 1:400), Alexa Fluor 555 goat anti-rabbit IgG (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... The Triton-X permeabilization solution was removed and cells were washed 3×2 minutes with DPBS at RT before a blocking solution (1% BSA (Fisher Scientific, BP1600-100) was added to cells for one hour at RT with rocking ...
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bioRxiv - Microbiology 2024Quote: ... After lysis of red blood cells with Gibco ACK lysing buffer and blocking of Fc receptors (Invitrogen CD16/CD32 clone 93, RRID AB_467133), cells were stained with live/dead aqua (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.1% Triton X in phosphate-buffered saline (phosphate-buffered saline (PBS)) and ReadyProbesTM Mouse-on-Mouse IgG Blocking Solution (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the following secondary antibodies diluted in blocking solution were applied for 45 min in the dark: Alexa Fluor 488-conjugated α-mouse IgG (Molecular Probes #A11001), 1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... the following secondary antibodies were incubated for 1 hour in blocking solution and protected from light: Alexa Fluor 488-conjugated α-mouse IgG (1:500, Molecular Probes #A11001), Alexa Fluor 488-conjugated α-mouse IgG1 (1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... 10 μL of peptide digests were loaded onto a C18 PepMap trap column (300 μm inner diameter x 5 mm, Thermo Fisher Scientific) at a flow rate of 30 μL/min ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulted peptides were separated by a LC gradient of two hours and analyzed on a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). The MS data were searched against the mouse database using the MaxQuant software ...
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bioRxiv - Cancer Biology 2021Quote: ... An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18, 5 μm particles, 20 mm length, Thermo Fisher Scientific) at 8 μL/minute ...
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bioRxiv - Cell Biology 2020Quote: ... for 120min at 300nl/min was used to elute peptides from the capillary reverse-phase column (0.075 x 250mm, Pepmap®, Fisher Scientific, 164941). Data were acquired using the Xcalibur software (version 4.0) ...
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bioRxiv - Genetics 2021Quote: The coding sequence for the pH insensitive Citrine fluorescent protein was inserted in the coding sequence of the BK2L3 protein (Zm00001d034049) immediately after the N-terminal secretory peptide domain and synthesized in the pDONOR221 vector (Life Technologies, USA) (Tian et al. ...
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bioRxiv - Genetics 2021Quote: ... peptides were analyzed by liquid chromatography tandem mass spectrometry (LC MS/MS) with an Easy-nLC 1000 (Thermo Fisher, San Jose, CA) coupled to an Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... A volume corresponding to 400ng of peptides was used for the analysis using an LC 1200 ultra-high-pressure system (Thermo Fisher Scientific) coupled via a nano-electrospray ion source (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... An aliquot of each peptide preparation was injected onto a PepMap RSLC C18 analytical column (2 µm, 100 Å, 75 µm x 25 cm, Thermo Scientific) and subject to a 90 min ...
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bioRxiv - Developmental Biology 2020Quote: ... peptides were first loaded onto a trapping cartridge (Acclaim PepMap300 C18, 5 μm particle size, 300 Å pore size, Thermo Fisher Scientific) and washed for 3 min with 0.1 % TFA ...
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bioRxiv - Developmental Biology 2020Quote: ... Peptides were ionized by the application of a 2.5 kV positive voltage applied distally to the peptides eluting into a Q-Exactive Plus (QE+) mass spectrometer (Thermo Scientific, Waltham, MA). Full MS spectra were recorded on the eluting peptides over a 350 to 1700 m/z range at 70,000 resolving power ...
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bioRxiv - Biochemistry 2020Quote: ... and washed for 3 min at 30 μL/min before peptides were eluted onto a C18 AcclaimTM PepMapTM column (100 Å, 5 μm × 0.75 mm × 150 mm, Thermo Fisher Scientific) at flow rate of 0.3 μL/min ...
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bioRxiv - Biochemistry 2022Quote: ... approximately 0.15 μg of peptides were run by online reversed phase chromatography on an Agilent 1290 UHPLC or Dionex UltiMate 3000 (Thermo Fisher Scientific) coupled to a Thermo Scientific Orbitrap Fusion mass spectrometer ...
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bioRxiv - Molecular Biology 2021Quote: ... peptides were analyzed in triplicate using an Ultimate 3000 nano coupled in line with an Orbitrap ELITE (Thermo Scientific, San Jose California). Briefly ...
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bioRxiv - Immunology 2021Quote: ... The peptides from the Hemocyte samples collected at four different time points were labelled with four 8-plex iTRAQ reagents (Applied Biosystems, USA).
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bioRxiv - Systems Biology 2020Quote: ... Up to 1mg peptide was fractionated by reverse-phase high pressure liquid chromatography (HPLC) on an Dionex UltiMate 3000 HPLC system (Thermo Scientific, USA) equipped with an XBridge® BEH C18 Sentry Guard Cartridge pre-column (130Å ...
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bioRxiv - Cell Biology 2019Quote: LC/MS data of the trypsin-Partially-digested peptides were collected on an Orbitrap Fusion mass spectrometer (Thermo Scientific, San Jose, CA) with a Nanoacquity M Class UPLC (Waters Corporation ...
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bioRxiv - Microbiology 2019Quote: ... The peptides obtained after trypsin digestion were analyzed by Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) analyzer (Applied Biosystems, USA). The tandem mass spectrometry (TOF/TOF ...
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bioRxiv - Cell Biology 2019Quote: Liquid chromatography mass spectrometry analysis (LC-MS / MS) of peptide extracts was done using 3000 Nano LC nanochromatographic system (Thermo Scientific, USA) and Maxis Impact mass spectrometer with an electrospray ionization source Captive Spray (Bruker ...
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bioRxiv - Cell Biology 2020Quote: ... The dried peptides were dissolved with 100 mM triethylammonium bicarbonate and then labeled with 6-plex Tandem Mass Tags (TMT) reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... at 37°C with 5% CO2 and transfected by different expression constructs for human IgG-Fc with various signal peptide coding regions using LipofectAmine (Invitrogen, Calsbad, CA) as the transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Eluted peptides were introduced directly via a nanospray ion source into a Q Exactive Plus hybrid quardrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). The mass spectrometer was operated in standard data dependent mode ...
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bioRxiv - Molecular Biology 2019Quote: ... Peptides were separated on reversed phase C18 columns (precolumn: Acclaim PepMap 100, 300 μm × 5 mm, 5μm, 100 Å (Thermo Fisher Scientific); separation column ...
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bioRxiv - Biochemistry 2019Quote: ... The peptides were loaded on a reversed-phase trap column (75 μm × 2 cm, 3 μm, C18, 100 Å, Thermo Scientific, Germany), and then the eluent was transferred to a reversed-phase analytical column (50 μm × 150 mm ...
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bioRxiv - Immunology 2021Quote: TMT-labelled tryptic peptides were subjected to pH reversed-phase (HpRP) fractionation using an Ultimate 3000 RSLC UHPLC system (Thermo Fisher Scientific) equipped with a 2.1 mm internal diameter (ID ...
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bioRxiv - Immunology 2021Quote: ... peptide separation using acetonitrile gradient was run for 120 min and (ii) data was collected on an Orbitrap Fusion (Thermo Fisher Scientific) operated at 120,000 resolution using HCD (higher-energy collisional dissociation ...
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bioRxiv - Neuroscience 2020Quote: ... At this stage the samples included in each 10plex were merged and were fractionated by basic Reverse Phase (bRP) using Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific), generating eight fractions for each of the two 10plexes.
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bioRxiv - Biochemistry 2020Quote: ... Peptides were loaded into a PepMap C18 (5 mm × 300 μm ID, 5 μm particles, 100 Å pore size; Thermo Fisher Scientific) trap column on an UltiMate 3000 RSLC nano HPLC system (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were digested with the filter-aided sample preparation (FASP) method23 and peptides were labeled using TMT 11-plex isobaric reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...