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Citations for Thermo Fisher :
4501 - 4550 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The peptide samples were loaded onto an Acclaim PepMapTM 100 C18 trap column (100 µm x 2 cm, 100 A; Thermo Scientific) and resolved on a PepMap RSLC C18 analytical column (2µm ...
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bioRxiv - Neuroscience 2024Quote: ... sonicated and the peptide concentration was determined through absorbance at 280 nm measurement using a Nanodrop instrument (ThermoFisher, Nanodrop One C).
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bioRxiv - Cell Biology 2024Quote: ... LC-MS analyses of peptide samples were performed on a hybrid ion trap- Orbitrap mass spectrometer (LTQ-Orbitrap Velos, Thermo Scientific) coupled with nanoflow reversed- phase liquid chromatography (EASY-nLC 1200 ...
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bioRxiv - Cell Biology 2024Quote: ... 400 µg of plasma exosome peptides (pooled from 10 different plasma exosome samples) were injected onto Dionex Ultimate 3000 LC (Thermo Scientific) coupled to ACQUITY UPLC CSH1.7 mm C18 column (2.1 × 150 mm ...
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bioRxiv - Immunology 2024Quote: ... were immunized subcutaneously at two sites on the back flank area with 200 μg of MOG35–55 (MEVGWYRSPFSRVVHLYRNGK) peptide (Thermo Fisher) emulsified in 200 μl incomplete Freund’s adjuvant (BD bioscience ...
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bioRxiv - Developmental Biology 2024Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 minutes organic gradient ...
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bioRxiv - Microbiology 2024Quote: Peptides were analysed through Ultimate 3000 RSLC nano-UPLC system connected with an Orbitrap Elite hybrid mass spectrometer (Thermo Fisher Scientific). Roughly 600 ng of peptides were loaded with automated injections onto a trap column (Acclaim PepMapTM 100 ...
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bioRxiv - Biochemistry 2024Quote: ... was used as a reference protein database and peptide spectra matches were performed using the SEQUEST algorithm within Proteome Discoverer v.2.1 (Thermo Fisher Scientific) with a fragment tolerance of 0.6 Da and parent tolerance of 10 ppm ...
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bioRxiv - Cancer Biology 2021Quote: ... in blocking buffer were incubated overnight at 4°C followed by washing and incubation with Alexa Fluor conjugated secondary antibodies (Invitrogen, 1:1000 dilution) at room temperature for 1-2 hour in the dark ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and incubated 1 hour at RT with secondary antibodies diluted in blocking solution as follows: anti-guinea pig Dylight 488 (Thermo Scientific, SA5-10094) (0.5 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Staining media was prepared in blocking buffer using alpha-Bungarotoxin (BTX) conjugated with Alexa Fluor 555 (ThermoFisher, B35451, 1:200 dilution in PBS). Additionally ...
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bioRxiv - Molecular Biology 2021Quote: ... blocked with 600 μL blocking buffer (33% sonication buffer, 67% 2x hybridization buffer, 500 ng/μL yeast RNA [Thermo Fisher Scientific], 1% BSA) at room temp for 1 hr ...
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bioRxiv - Neuroscience 2019Quote: ... slices were washed in PBS and then incubated in PBS blocking buffer containing goat anti-rabbit 2o antibody conjugated with an Alexaflour 594 (Life Technologies, 1:500) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... slices were washed in PBS and then incubated in PBS blocking buffer containing goat anti-rabbit 2o antibody conjugated with an Alexafluor 594 (Life Technologies, 1:500) for 1 hour at room temperature.
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bioRxiv - Physiology 2020Quote: ... Slides were subsequently exposed to a 1:5000 dilution of the primary antibody in blocking solution (Alexa488-conjugated rabbit anti-GFP; Life Technologies, Waltham, MA) for 3 hours at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... in blocking buffer were incubated overnight at 4°C followed by washing and incubation with Alexa Fluor conjugated secondary antibodies (Invitrogen, 1:1000 dilution) at room temperature for 1-2 hour in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then washed 3 times in PBS and incubated with appropriate fluorochrome secondary antibody diluted in blocking solution for 1-2h (AlexaFluor® 488, AlexaFluor® 568, 1:400, Life Technologies). Finally ...
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bioRxiv - Bioengineering 2020Quote: ... Slides were blocked in serum-based blocking buffer and incubated with primary antibody against MMP12 (MA5-32011, Invitrogen, 1:50, 20 μg/mL). Slides were washed in PBS (3×5 minutes) ...
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bioRxiv - Biophysics 2021Quote: ... After one hour of blocking with 5% (w/v) skim milk in 0.1% (v/v) Tween™-20 (Fisher Scientific, Hampton, NH, USA) and PBS (PBS-T) ...
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bioRxiv - Cancer Biology 2019Quote: ... the sections were incubated overnight at room temperature with the indicated primary antibody prepared in blocking/permeabilization solution: mouse monoclonal antibody against HK-2 (1:100; Thermo Fisher, MA5-15679) or mouse monoclonal antibody against GLUT-3 (1:100 ...
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Pax6 loss alters the morphological and electrophysiological development of mouse prethalamic neuronsbioRxiv - Developmental Biology 2021Quote: ... cells were washed with 0.1% PBST and further incubated with blocking serum containing the corresponding secondary antibodies (Streptavidin Alexa Fluor™ 488, 546 or 647 conjugates; Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated in 1 mL the secondary staining solution (goat anti-rabbit-AlexaFluor 488 [Thermofisher] at 1:1000, Hoechst 33342 at 1:1000 [Thermofisher] and Phalloidin-AlexaFluor555 at 1:200 [Thermofisher] in blocking buffer). The tissue was washed extensively and then mounted on glass slides using VectaShield (Vector Labs ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then permeabilized using 0.1%Triton X-100 for 10 minutes and blocked using Seablock blocking buffer (37527, Thermo Fisher Scientific, MA, USA) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Hemocytes were washed three times with blocking buffer and counterstained with 20 µM Hoechst 33342 (405, Molecular Probes, ThermoFisher Scientific, Waltham, MA, USA) and then mounted by adding 2 drops of Prolong Gold Antifade Mountant (Molecular Probes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with primary antibodies diluted with blocking buffer overnight at 4°C followed by washes and incubation with Alexa Fluor®-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... the secondary antibody (1/400 in blocking buffer, goat anti-rabbit Alexa 594 antibody, Thermo Fisher, A-11037 or donkey anti-mouse Alexa 488 antibody, Thermo Fisher, R37114) was added in blocking buffer for 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... a secondary antibody was applied to the sections for 2 hours (AlexaFluor goat anti-mouse 594 diluted 1:400 in blocking buffer; Life Technologies, Carlsbad, CA). Sections were again washed in PBS over 30 minutes prior to the application of mounting media and coverslips.
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bioRxiv - Developmental Biology 2022Quote: ... The samples were washed (4 times 30 min, PBST) and then incubated with the secondary antibody (Alexa fluor 647, 1: 1200 ThermoFisher in blocking solution) overnight at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Then samples were incubated for 30 min in blocking buffer containing 2% (w/v) BSA and then incubated with Alexa Fluor 488 phalloidin (1:400 dilution, Thermo Fisher Scientific, A12379) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... GFP was amplified with an anti-chicken GFP antibody from Abcam and retina slices were incubated overnight with primary antibodies in blocking solution followed by washes in PBS and a 3-hour incubation with anti-isotypic secondary antibodies (Invitrogen or Jackson Immunoresearch). Labeling of Dact2-GFP or Plcxd2-GFP retinas for GAD67 (mouse monoclonal ...
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bioRxiv - Developmental Biology 2020Quote: ... sections were rinsed in 0.02M KPBS and incubated for 2 hr at room temperature in blocking buffer containing goat anti-rabbit Alexa-Fluor conjugated secondary antibodies (Life Technologies, Carlsbad, CA). Sections were coverslipped using ProLong antifade mounting medium (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated for 2 hr at room temperature in blocking buffer containing secondary antibodies against rabbit (raised in goat) conjugated with Alexa-Fluor fluorochromes (Life Technologies, Carlsbad, CA) and coverslipped using ProLong mounting medium (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated overnight (4°C) in the same blocking solution containing the primary rabbit anti-PV antibody (1:1000; Thermo Scientific, PA1-933) and mouse anti-GFP antibody (1:500 ...
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bioRxiv - Genetics 2019Quote: ... primary antibodies in blocking buffer containing 5% milk followed by the secondary antibodies of goat anti-mouse IgG (H+L) HRP (Life Technologies, 1:10000) or goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2019Quote: ... washed with PBS and then blocked with blocking solution composed of 10% normal donkey serum or normal goat serum (both from Thermo Fisher Scientific Inc.) and 0.1% Triton-X-100 in PBS for one hour at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated for 30 minutes with the appropriate secondary antibodies (1:400 dilution in blocking buffer) and/or Alexa FluorTM-647 conjugated phalloidin (1:400, Invitrogen, A22287, lot 1884190), and washed twice more with PBS ...
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bioRxiv - Biochemistry 2019Quote: ... Sections were washed 3x in 1x PBS and bound primary antibodies were detected by incubation with Alexa-fluor conjugated secondary antibodies diluted in blocking buffer (Thermo Fisher/Invitrogen, Eugene, OR) for 1 hr at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then washed with PBS and incubated with blocking buffer (10% goat serum in PBS) for 30 min at RT and incubated with primary antibody (anti-GFP Invitrogen chicken Y fraction) at 1:1000 in blocking buffer 4 h RT ...
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bioRxiv - Biochemistry 2022Quote: ... coverslips were washed 3 x 10 min in PBS containing 0.1% Triton X-100 at room temperature and then incubated for 1 hr at room temperature with secondary antibodies diluted in blocking buffer: AlexaFluor594 goat anti-mouse IgG (Molecular Probes, ThermoFisher Scientific, AB_2534091) and AlexaFluor488 Streptavidin (Molecular Probes ...
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bioRxiv - Bioengineering 2022Quote: ... Organoids sections were washed three times for ten minutes in 1X PBS prior to a two-hour incubation in 10% BSA in PBS blocking solution (ThermoFisher Scientific BP1605-100). The sections were then incubated in primary antibodies diluted in blocking solution overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and lung cryosections after blocking were stained with a 1:200 dilution of Alexa Fluor 488 Phalloidin (Thermo Fisher Scientific, Rockford, IL, USA) for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Virus infected cells were detected by staining with a primary rabbit anti-VACV antibody (1:1000 in blocking buffer for 1 hour, Thermo Fisher PA1-7258) and a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (1:1000 in blocking buffer for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cells were washed 5x with blocking solution and mounted with Invitrogen ProLong™ Gold antifade reagent with DAPI (Thermo Fisher, MA, USA). Slides were imaged with an Olympus FV3000 confocal microscope.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cells were washed 5x with blocking solution and a 1:500 dilution of secondary antibodies (Invitrogen Goat anti-rabbit IgG (H+L) Superclonal™/Invitrogen Alexa Fluor™ 594 goat anti-mouse IgG2a (γ2a ...
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bioRxiv - Molecular Biology 2023Quote: ... The following secondary antibodies were added overnight at 4°C in blocking buffer: Alexa Fluor™ 647 donkey anti-rabbit IgG (1:1000, Invitrogen, #A-32795), Alexa Fluor™ 488 donkey anti-mouse IgG (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were washed for 1 hour in 1x PBS twice at room temperature and then incubated in a solution containing 1% Triton X-100 (93443, MilliporeSigma) in StartingBlock (PBS) Blocking Buffer (37538, ThermoFisher Scientific, Waltham, MA) overnight at room temperature to permeabilize and block the tissue ...
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bioRxiv - Neuroscience 2022Quote: ... the fixed tissue cultures were incubated for 1 h at room temperature in blocking solution consisting of 10 % (v/v) normal goat serum (NGS; Fisher Scientific, Cat# NC9270494) and 0.5 % (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at RT and then in the blocking buffer containing monoclonal anti-GFP mouse antibody (GF28R, 1:3000, Thermo Fisher, #MA5-15256) and biotin-conjugated anti-ALFA nanobody (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed with blocking solution and incubated with species-specific Alexa Fluor 488 and 555 conjugated secondary Abs (1:200) (Invitrogen, Carlsbad, CA, USA) and DR™ nuclear stain (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... they were incubated with the secondary antibodies diluted in blocking solution: Alexa Fluor 488-conjugated goat anti-chicken IgY (A-11039, Invitrogen, dilution 1:1000) and Alexa Fluor 594-conjugated goat anti-mouse IgG (A-11020 ...