Labshake search
Citations for Thermo Fisher :
4551 - 4600 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... Usually 5 µg of total protein or the total protein of 1 to 2 × 106 autophagosomes were subjected to 4 – 12 % NuPage Bis-Tris gels (Thermo Scientific, MP0335) and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo RTA mini nitrocellulose transfer kit (BioRad ...
-
A stable reference human transcriptome and proteome as a standard for reproducible omics experimentsbioRxiv - Cell Biology 2022Quote: ... Cells were dissolved in 1% SDS lysis buffer (Beyotime, P0013G, China) and the protein concentration was measured using a BCA kit (ThermoFisher, 23227, USA). The protein digestion was performed by filter-aided sample preparation (FASP ...
-
bioRxiv - Cell Biology 2022Quote: ... Treated cells were incubated for 1 and 4 hours and total protein was isolated using RIPA lysis and extraction buffer (Thermo Scientific, 89900) and run using SDS-PAGE in 10% precast polyacrylamide gels (BioRad ...
-
bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
-
bioRxiv - Genetics 2022Quote: ... Blots were blocked for nonspecific protein binding with 5% (w/v) nonfat milk and then incubated with a 1:500-diluted antibody against COL15A1 (Thermo Fisher Scientific) or 1:3000-diluted antibody against GAPDH (glyceraldehyde 3-phosphate dehydrogenase ...
-
bioRxiv - Microbiology 2022Quote: ... proteins were separated on Bolt 4-12% Bis-Tris gel in 1 × Bolt MOPS SDS running buffer (Thermo Fisher Scientific catalog # B0001). Separated proteins were electro-transferred to nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Microbiology 2022Quote: ... or with 200 μL of Exosome Resuspension Buffer (1-2.5% Triton X-100) from the Total Exosome RNA and Protein Isolation Kit (Invitrogen, Thermo-Fisher Scientific) to lyse the EVs for proteomic analysis.
-
bioRxiv - Microbiology 2022Quote: ... Yeast were washed twice with ice-cold PBS-BSA and fluorescently labeled for 1 hr at 4°C with 1:100 FITC-conjugated chicken anti-Myc (Immunology Consultants CMYC-45F) to detect yeast-displayed RBD protein and 1:200 PE-conjugated streptavidin (Thermo Fisher S866) to detect bound ACE2 ...
-
bioRxiv - Physiology 2024Quote: Titin isoforms were separated on a 1% w/v agarose gel and stained with SYPRO Ruby protein gel stain (Invitrogen, ThermoFisher Scientific) as previously described.30 All samples were measured in triplicate and the average of triplicate measurements per sample was shown.
-
bioRxiv - Microbiology 2024Quote: ... coli type I fimbria or FliC (diluted 1:10 000 in protein-free tris-buffered saline (TBS) blocking solution (Fisher Scientific, France)) for 1 h at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... 1h at RT and subsequently incubated overnight at 4°C in primary antibody solution using Rabbit anti-Green-Fluorescent-Protein (GFP; 1:1000 ThermoFisher Scientific, A6455). The following day sections were rinsed three times in PBTriton 0.5% and incubated overnight at 4°C in secondary antibody solution using Goat anti-Rabbit-Oregon Green 488 (1:500 Invitrogen ...
-
bioRxiv - Synthetic Biology 2023Quote: After washing with PBSF (PBS+1% BSA), the cells were incubated with 1uM biotinylated target proteins (IGF-2R, ASGPR, Sortilin) together with streptavidin–phycoerythrin (SAPE, ThermoFisher, 1:100) and anti-c-Myc fluorescein isothiocyanate (FITC ...
-
bioRxiv - Genetics 2022Quote: ... The 10 μL Hifi Cas9 protein were aliquoted into 10 tubes followed by 1:5 dilution with Opti-MEM (ThermoFisher Scientific, 31985062) solution before use.
-
Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 1% FBS in PBS) and the transfer of CFSE labeled proteins was analyzed by an AttuneTM NxT flow cytometer (Thermo Fisher Scientific) and FlowJo software version 10.7.1 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were co-transfected at ∼80% confluence with wild-type (4 μg) or mutant SCN1A construct (4 μg) and enhanced green fluorescent protein (eGFP (1 μg) using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) and incubated under the culture conditions above for 24 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... Immunocomplexes were isolated with protein A/G beads (Thermo, 20421) and eluted 1× LDS sample buffer with 10% β-mercaptoethanol (Invitrogen; NP0007) at 55 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... They were then incubated at 4 °C overnight with primary antibody rabbit anti-Myosin VI (1:400 Proteus Bioscience) and mouse anti-green fluorescent protein (Invitrogen, cat. A11120). The tissues were incubated with 1:800 diluted secondary antibodies for 2 hrs at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were then stained with primary antibodies overnight at 4°C targeting the following proteins: MAP2 (PA1-10005, ThermoFisher Scientific, 1:5000), AT8 (MN1020 ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.5 mL of a 1–2 mg/mL protein sample was mixed with 2.5 µL of SYPRO® orange dye (ThermoFisher Scientific, USA) and aliquoted at 5 µL before being mixed with 45 µL of the respective screening buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated overnight with SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) rabbit polyclonal antibody (Thermo Fisher scientific, France) at a dilution of 1:1000 in the same buffer as for saturation ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by an incubation of 100 μl of a 2:1 (A:G) mixture of Dynabeads Protein A and G (Invitrogen, 10001D and 10004D) for 2h at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were incubated on ice for 15 min and protein expression was initiated with the addition of 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Fisher Scientific; BP1755). Cells were then incubated for 16–18 hr at 18°C for optimal protein production.
-
bioRxiv - Molecular Biology 2023Quote: ... Cell extracts were incubated with 12 μg anti-PRG-1 antibody (15) and 60 μl Protein A/G dynabeads (Thermo Fisher Scientific) at 4°C for 3 h ...
-
bioRxiv - Neuroscience 2023Quote: ... for 1 h at 4°C before the addition of 25 μL of Pierce Protein A/G Magnetic Beads (Thermo Fisher Scientific) for an additional 2 h under constant rotation ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Yeast were washed twice with ice-cold PBS-BSA and fluorescently labeled for 1 hr at 4°C with 1:100 FITC-conjugated chicken anti-Myc (Immunology Consultants CMYC-45F) to detect yeast-displayed RBD protein and 1:200 PE-conjugated streptavidin (Thermo Fisher S866) to detect bound ACE2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... lysates were incubated for overnight at 4°C with 2 μg of antibodies recognizing MTR4 or IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A (ThermoFisher Scientific). After incubation ...
-
bioRxiv - Microbiology 2023Quote: ... Then each sample was diluted in a 1:10 ratio and measured by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... the microtiter plates were washed 5 × with T-PBS and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of IgG antibodies from human ...
-
bioRxiv - Immunology 2024Quote: ... with T-PBS and then incubated for 1 hour at room temperature with 2 µg/mL biotinylated Protein-G (Thermo Fisher Scientific) in T-PBS ...
-
bioRxiv - Molecular Biology 2024Quote: ... and non-specific IgG were incubated for 1 hour at room temperature with 20 µL aliquots of Protein A Dynabeads (Thermo Fisher, USA) mixed with 200 µL of PBST ...
-
Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: To confirm protein enrichment on the pulldown samples the protein were eluted from the beads as mentioned above and 1/3rd of the eluted protein was resolved on a 4–12% Bris-Tris gel (Invitrogen: cat# NW04125box) and transferred onto transferred onto iBlot 2 Transfer Stack containing nitrocellulose membrane using the BOLT transfer system ...
-
bioRxiv - Neuroscience 2024Quote: ... Phosphorylation was induced by incubating 1 µg of recombinant human polo-like kinase 3 (PLK3) protein (Thermo Fisher Scientific, MA, USA; #PR7316B) with 50 µL of mouse WT α-synuclein in kinase reaction buffer for 8 hrs at 30°C ...
-
bioRxiv - Biochemistry 2024Quote: ... or 1.9 mg-protein mL-1 Pd-CI-NDs (with FOM) were applied to each grid in an FEI Vitrobot IV (Thermo Fisher Scientific) at 4 °C and 100% relative humidity before blotting for 10 s with a blot force setting of - 10 and vitrifying the sample in liquid ethane ...
-
bioRxiv - Molecular Biology 2024Quote: The following primary antibodies were used for the immunological detection of specific proteins on PVDF membranes: anti-Tap (CAB1001 rabbit polyclonal antibody, Invitrogen, 1:10.000), anti-HA (12CA5 mouse monoclonal antibody ...
-
bioRxiv - Cancer Biology 2021Quote: ... respectively coupled with protein G Dynabeads (Invitrogen) with nuclear extract derived from MCF-7 cells for 2 hours ...
-
bioRxiv - Cancer Biology 2021Quote: ... Dynabeads Protein A or G (Life Technologies), 25 µl per IP ...
-
bioRxiv - Bioengineering 2021Quote: ... Dynabeads M-280 Protein G (ThermoFisher, 10003D) were resuspended by vortexing for 30 s ...
-
bioRxiv - Biophysics 2020Quote: ... B-PER Bacterial Protein Extraction Reagent (ThermoFisher) was used to lyse the cells in the presence of protease inhibitor ...
-
bioRxiv - Cancer Biology 2021Quote: ... or DynabeadsTM protein G beads (#10004D, Invitrogen) were added into each sample and incubated at 4 °C for 1-2 hours on the next day ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein G Dynabeads (Thermo Fisher Scientific, #10003D), protein G beads (EMD Millipore ...
-
bioRxiv - Cancer Biology 2021Quote: ... Sypro Ruby Protein Blot Stain (Molecular Probes) was used to quantify protein concentration in each sample ...
-
bioRxiv - Cell Biology 2020Quote: ... Dynabead Protein G beads (ThermoFisher, Waltham MA) were added to a concentration of 30 µL/mL and rotated for 2 hours at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: 100 µl of Dynabeads Protein A (ThermoFisher) were prepared for immunoprecipitation (IP ...
-
bioRxiv - Immunology 2021Quote: ... Protein concentrations was determined by Thermo Fisher BCA assay kit and then 50μg of protein was loaded into the pre-coated VEGFA ELISA kit (R&D systems) ...
-
bioRxiv - Genetics 2021Quote: ... 30 pmol TrueCut Cas9 Protein (Invitrogen #A36498) and 15 pmol gRNA were combined for a ratio of 2:1 ...
-
bioRxiv - Genetics 2021Quote: ... Dynabead protein A (50ul; Thermo Fisher 10001D) and protein G (50ul ...
-
bioRxiv - Microbiology 2019Quote: ... bound to protein A magnetic beads (Invitrogen). After three washes with 1X IP buffer ...
-
bioRxiv - Developmental Biology 2021Quote: ... were conjugated with Dynabeads Protein G (Thermofisher) magnetic beads ...
-
bioRxiv - Developmental Biology 2020Quote: ... 20 μl of protein G Dynabeads (Invitrogen) per aliquot were washed twice with ChIP dilution buffer and resuspended in 50 μl/aliquot of the same solution ...
-
bioRxiv - Developmental Biology 2021Quote: ... Dynabeads Protein G (10003D, Thermo Fisher Scientific) and rat anti-HA antibody (3F10 ...