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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The protein concentration was determined in the samples using the Pierce BCA Protein Kit (Thermo Fisher Scientific Inc). The solid protein was denatured at 95°C for 5 min in NuPAGE LDS Sample Buffer (4X ...
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bioRxiv - Immunology 2024Quote: ... 0.6 µg gRNA was mixed with 2 µg Cas9 protein (TrueCut™ Cas9 Protein v2, Thermo Fisher Scientific) in 5 µl E8 medium supplemented with 10 μM Y27632 ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration of lysates was tested by the Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific). For the reducing gel ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants (2 mg protein) were removed and incubated overnight at 4°C with Dynabeads Protein G (Thermo Scientific) coated with anti-FLAG antibody or IgG heavy chain according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein fractions were quantified using an 8-point Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, PI23225), and 20 µg of protein per sample diluted to 35µL using NuPAGE LDS sample buffer (1x final conc. ...
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bioRxiv - Molecular Biology 2024Quote: ... protein concentrations of sheared chromatin were quantified with the Pierce™ BCA Protein Assay Kit (Thermo Scientific, #23227) following manufacturer instructions.
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bioRxiv - Bioengineering 2024Quote: ... Total protein concentration was quantified by Micro BCA Protein Assay Kit according to the manufacturer’s instructions (Thermo Scientific). Samples and migration standards were prepared under reducing conditions and incubated for 10 min at 37 °C prior to loading ...
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bioRxiv - Physiology 2024Quote: ... The protein concentration in the supernatant was then determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). For protein analysis in isolated mitochondria ...
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bioRxiv - Developmental Biology 2024Quote: ... The supernatant was kept it in ice and protein concentration were measured with QUBIT protein kit (#Q33211, ThermoFisher).
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bioRxiv - Developmental Biology 2024Quote: ... The amount of protein in the lysate was quantified by NanoOrange™ Protein Quantitation Kit (Thermo Fisher Scientific) and the enzymatic activity of glutamine synthetase was measured by Glutamine Synthetase Activity Assay Kit (Abcam) ...
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bioRxiv - Developmental Biology 2024Quote: ... The lysate protein content was quantified with the Pierce™ BCA Protein Assay Kits (ThermoFisher Scientific, Cat.#: 23225) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by SDS-PAGE on NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gels (Invitrogen) in MOPS running buffer ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The EV protein concentration was measured by using a Micro BCA Protein Assay Kit (23235, Thermo Fisher Scientific). The collection method ...
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bioRxiv - Cancer Biology 2024Quote: ... and estimated the protein concentrations using Pierce BCA Protein Assay Kit as per the manufacturer’s protocol (ThermoFisher Scientific). Around 10 µg of proteins were allowed to resolve in 8% SDS-PAGE separating gel ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, cat. no. 23225). The concentration of samples was adjusted by dilution with RIPA buffer ...
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bioRxiv - Plant Biology 2019Quote: The DNA coding sequences for the 20 fluorescent protein (FP) genes listed in Table 1 were mobilized into Invitrogen pENTR /D-TOPO cloning vectors (Invitrogen, Carlsbad, CA). Following colony PCR and validation by sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... Tubulin beta chain protein was detected with an anti-TUBB antibody (1:500 in 5% fat-free milk solution in TBST, Invitrogen MA5-16308) followed by an anti-mouse secondary antibody (Alexa Fluor™ Plus 800 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were blocked with 5% milk in TBST buffer and were probed with antibodies diluted in 5% BSA in TBST buffer against the following proteins: FCHSD2 (#PA5-58432, 1:500, Thermo Fisher Scientific), Rab11 (#5589S ...
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bioRxiv - Molecular Biology 2022Quote: ... Ago2 and IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A or G (ThermoFisher Scientific). After incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... The diluted protein was coated in an increasing gradient (25-200 ng well-1) on a 96-microtiter ELISA plate (Nunc, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... SEMA3C protein levels were checked by western blot as described above using rabbit anti-human SEMA3C antibody (1:1000, Thermo Fisher Scientific). Beta-actin (housekeeping gene ...
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bioRxiv - Developmental Biology 2019Quote: ... and biotinylated at a molar ratio biotin/protein (3:1) for 30 min at room temperature (EZ-Link NHS-PEG4-Biotin (1189-1195, Fisher Scientific, Germany)) ...
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bioRxiv - Cell Biology 2020Quote: ... AHA-labeled proteins were detected with 1 μM alkyne-tagged Alexa Fluor-488 using Click-iT cell detection reagents (Thermo Fisher Scientific) for 45 min at room temperature as previously described (3).
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bioRxiv - Genetics 2019Quote: Coverslips were incubated for one hour at 37°C in 1mL of of 1:30 solution of Collagen I Rat Protein (Thermo Fisher Scientific) to 1X PBS ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatant was collected and diluted to 1 µg/µl in KHM buffer after determination of its protein concentration by the Pierce BCA assay (Thermo Fisher Scientific). For immunoprecipitation ...
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bioRxiv - Neuroscience 2021Quote: Bacterial expression constructs coding for His6/SUMO-tagged fusion proteins of rat Shank3 (residues 1-348, SPN-Ank) were generated in pET-SUMO (Thermo Fisher Scientific) as described before (17) ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations were adjusted to 1-3 μg/μl in lysis buffer and NuPAGE 4x LDS sample buffer (NP0007) (Thermo Fisher Scientific) was added to lysates ...
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bioRxiv - Cell Biology 2021Quote: ... An aliquot of lysate was taken and diluted 1:5 in 8 M Urea from which protein concentration was determined by BCA assay (Thermo Fisher Scientific). Aliquots corresponding to 100 μg of protein were subsequently diluted in SDC buffer into a 96-well deep-well plate ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was incubated at 4°C for 1 h with anti-BmAgo3 antibody23 conjugated to Dynabeads protein G (Thermo Fisher Scientific). The superparamagnetic beads were washed five times with wash buffer (30 mM HEPES-KOH ...
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bioRxiv - Immunology 2022Quote: Plasma was pooled and heat inactivated at 56°C for 1 hour then diluted in protein G binding buffer (Pierce ThermoFisher, catalog #54200) and passed over a column containing 1mL of protein A/G resin (Pierce ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... Yeast were washed with ice-cold PBS-BSA and fluorescently labeled with 1:100 FITC-conjugated chicken anti-Myc (Immunology Consultants CMYC-45F) to detect yeast-displayed RBD protein and 1:200 PE-conjugated streptavidin (Thermo Fisher S866) to detect bound ACE2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were denatured for 1 minute at 70°C and RNA-protein complexes were resolved on NuPAGE 4-12% Bis-Tris Gels (Thermo Fisher, #WG1402A) at 180V for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was dissolved in 200 µl 1% SDS with protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, Cat. No. 78444). Any insoluble material was removed by centrifugation at 10,000 x g for 10 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: TSA assays were performed by mixing 5 μL protein solution at 2 mg/mL with 5 μL SYPRO®Orange (1:50 diluted from 5000x stock solution, Invitrogen, USA), 5 μl of 8-fold buffer (1200 mM NaCl and 400 mM Tris pH 8) ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then stained with 20 µg/mL biotinylated S protein (S1/S2) or 1:1000 Anti-HS (Clone F58-10E4) (Fisher Scientific, NC1183789) in PBS containing 0.5% BSA ...
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bioRxiv - Biophysics 2020Quote: ... (67)) or end-binding protein 1 (EB1)-EGFP (EB1/GFP-3; (68)) were cultured in Life Technologies Opti-MEM (Invitrogen, Carlsbad, CA) containing 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... Human cell lysate and mouse cell lysate (as a spike-in internal control) were combined (∼4:1 ratio) and incubated with protein A or G Dynabeads (Thermo Fisher Scientific) conjugated with the relevant antibodies for 14 h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected after 6 days and passed through a gravity chromatography column containing 1 ml of PierceTM Protein G Agarose resin (ThermoFisher Scientific 20397). The column was washed with 20 ml of wash buffer (150mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... with 0.1% Tween 20 to assess endothelial VCAM-1 and E-selectin protein expression using the SuperPicture 3rd Gen Immunohistochemistry detection system (Invitrogen, Waltham, MA). Endogenous peroxidases were quenched and endogenous mouse IgG in blood serum was blocked using the Mouse-on-Mouse blocking reagent (Vector Laboratories ...
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bioRxiv - Genetics 2019Quote: ... Each sample containing equal amount of proteins was incubated overnight at 4 ℃ with mouse anti-V5 (1:500; Thermo Scientific, R960-25). Then ...
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bioRxiv - Pathology 2021Quote: Plasmids for expressing proteins having LFn (LF aa 1-254) fused to Cre recombinase were constructed using the Champion pET SUMO vector (Invitrogen, Carlsbad, CA), which expresses proteins fused at the C-terminus of a His6-SUMO tag ...
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bioRxiv - Microbiology 2019Quote: ... We matched MS/MS fragmentation spectra to human forward protein databases and against reverse databases to a 1–2% false discovery rate using the SEQUEST database search program (Thermo Fisher Scientific) [61] ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were treated for 1 hour with mock (untreated cells) or PNDD1 200 nM before fractionation with Subcellular Protein Fractionation Kit (#78840, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: 1 μg of IRE1-KR recombinant protein was crosslinked in 25 μl final volume of RNA cleavage buffer containing 1 μl of disuccinimidyl suberate (DSS, Thermo Fisher Scientific) crosslinker at 6.25 mM (final concentration is 250 μM ...
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bioRxiv - Cancer Biology 2020Quote: ... Chromatin fragments were immunoprecipitated overnight using 1 μg antibody of SOX11-PAb antibody and 20 μl Protein A UltraLink® Resin (Thermo Scientific) beads per 10×106 cells ...
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bioRxiv - Molecular Biology 2021Quote: ... for 14-17 h at 4°C followed by the incubation with 100 μl of Dynabeads Protein A + G (2:1 A:G proportion, Invitrogen, 10001D and 10004D) for 2 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Molecular Biology 2020Quote: ... normalized to 2 mg/ml and protein expression was analysed by western blotting employing anti-V5 antibody (1:3000 dilution, Invitrogen, #R960-25).
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with 1μg/ml of biotinylated recombinant ACE2 protein for 1 hour at room temperature followed by incubation with HRP-conjugated streptavidin (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary (4°C overnight) and secondary antibodies (1 h, room temperature) dilutions and proteins were visualized with enhanced chemiluminescence (ECL) (Pierce; Thermo Fisher Scientific). For loading control (Supplementary figures 3b and 3k) ...