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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Commercial colloidal force probes (CP-qp-CONT-BSA-A, NanoAndMore USA) were passivated with heat-inactivated fetal calf serum (FCS, Invitrogen) for 1h at room temperature to avoid non-specific adhesion of the bead to the cells ...
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bioRxiv - Pathology 2020Quote: ... cells were first synchronized overnight in 1% FCS DMEM and then cultured for 14 days in 10% FCS DMEM and stimulated daily with IL-1β (1 ng/ml; Invitrogen) or IL-17 at (2.5 ng/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Two x 106 cells/well of a 6-well plate were seeded in DMEM containing 10% FCS (Invitrogen, Carlsbad; USA), 1% HEPES (Carl Roth ...
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bioRxiv - Immunology 2019Quote: ... The biopsy samples were collected in basic medium supplemented with 10% FCS and minced into pieces of 1 mm3 in PBS (Gibco). The biopsies were enzymatically digested with 1 mg/mL collagenase IV (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 1% [v/v] FCS in PBS++) and primary antibodies were detected with secondary antibodies conjugated to Alexa Fluor dyes (Invitrogen). Images were acquired as above ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by addition of 0.5 N HCl and absorbance at 450 nm was measured using a Muktiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Neuroscience 2019Quote: ... with 10% fetal calf serum (FCS) at 37°C in an atmosphere with 5% CO2 and transfected with lipofectamine (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Fc Block was diluted in deficient RPMI (dRPMI; RPMI-1640 supplemented with HEPES and 1g/L sodium azide [ThermoFisher Scientific]) with 10% heat-inactivated FBS (dRPMI+FBS) ...
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bioRxiv - Biochemistry 2021Quote: ... and resuspended in plating medium (Dulbecco’s modified Eagle’s medium (DMEM)/medium 199 [4:1 (v/v)]) containing 15% (v/v) foetal calf serum (FCS; Life Technologies) and 100 U/ml penicillin and streptomycin ...
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bioRxiv - Immunology 2020Quote: ... washed 2x with PBS 0.5% FCS and then stained with the Click-it Plus OPP Protein Synthesis Kit Alexa Fluor 647 (C10458, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10 % foetal calf serum (FCS, Labtech) and 100 U/ml penicillin and 100 μg/ml streptomycin (Pen/Strep; Gibco). THP-1 cells were maintained in RPMI (Gibco ...
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bioRxiv - Immunology 2021Quote: ... cells from each vial were thawed in a water bath at 37°C and transferred to 10 mL thawing medium 10% FCS (Gibco) 1% Pen-Strep (Gibco ...
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bioRxiv - Immunology 2021Quote: ... or in sterile Sorting Medium [RPMI 1640 supplemented with 10% (v/v) Heat-Inactivated Fetal Bovine Serum (HI-FCS; A3840001; Gibco)] ...
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bioRxiv - Immunology 2021Quote: ... 1.25 × 105 J76 cells were co-incubated with 2.5 × 105 K562 cells transgenic for HLA-A*02:01 in 96 well plates filled with 200 μL of IMDM media 10% FCS (Gibco) containing serial dilutions peptide of interest in three independent replicates ...
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bioRxiv - Immunology 2022Quote: ... from healthy donors were isolated from whole blood by density centrifugation on Lymphoprep (Axis-Shield PoC) and cryopreserved at −150 °C in FCS (Gibco) + 10% DMSO.
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in complete B cell medium (RPMI supplemented with 10%FCS, 25 mM Hepes, Glutamax, penicilin streptomycin (Invitrogen) and 1‰ß-mercaptoethanol (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... Stop reaction was added in each well and protein was measured at 450 nm in a Multiskan FC (Thermo Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... UM-SCC-47 cell line was cultured in three parts of Hams F-12 nutrient mixture medium and one part of DMEM with 10% FCS (Gibco) and 100 U/mL penicillin–streptomycin ...
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bioRxiv - Molecular Biology 2022Quote: ... Hepatocytes were then maintained for up to 1 week in hepatocyte culture medium (L15 medium, 8.4% FCS + 1% glutamine + 10% tryptose phosphate, Life Technologies) containing 1 μM insulin ...
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bioRxiv - Immunology 2022Quote: ... the cells were washed thrice with PBS and Minimal Essential Medium α containing additional nucleosides and 10% FCS (Thermo Fisher) was added ...
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bioRxiv - Microbiology 2022Quote: ... and the absorbance at a wavelength of 595 nm was measured using a plate reader (Multiskan FC, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... The optical density was measured at wavelength 405 nm (OD405) by a microplate photometer (Multiskan™ FC, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: E14tg2a ESCs (RIKEN Cell Bank) were cultured in Glasgow minimum essential medium (GMEM; Wako) containing 10% fetal calf serum (FCS; Invitrogen), 1 mM glutamine (Wako) ...
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bioRxiv - Immunology 2024Quote: ... Tachyzoites were maintained in vitro by serial passages on confluent monolayers of HFF using DMEM supplemented with 1% FCS (GIBCO). For mouse infections ...
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bioRxiv - Immunology 2024Quote: ... A single dose of 10 plaque forming unit (PFU) of PVM in 10 µl or the same volume of vehicle (10%, v/v, FCS in DMEM; Gibco) was administered via the intra-nasal (i.n. ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood as described for the PBMCs from the patients and cryopreserved at -150°C in fetal calf serum (FCS, Gibco) with 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2024Quote: ... a final concentration of 0.1 μg/ml of VHH-Fc in PBS was mixed with 10X SYPRO Orange dye (Life Technologies). Dye binding to molten globule unfolding protein was measured over a 0.01 °C/s temperature gradient from 20 °C to 98 °C in a Roche LightCycler 480 qPCR machine.
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bioRxiv - Immunology 2023Quote: ... to block non-specific binding of fluorochrome-conjugated anti-human monoclonal antibodies to Fc receptors before being labeled with a 27-color panel of markers including the viability dye Live/Dead Blue (Invitrogen), following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: HeLa and HEK293T cells were grown in DMEM (Dulbecco’s modified Eagle’s medium) with GlutaMAX and 10% decomplemented-FCS (fetal calf serum) (Gibco, Life Technologies). HeLa WT and HeLa BST2 KO cells were a kind gift of Stéphane Fremont (Institut Pasteur ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then washed and incubated with 1 μg/ml DAPI/20% FCS/0.3% TritonX-100/PBS and either anti-rabbit Alexa Fluor 488 (Invitrogen, A11070) and anti-mouse Alexa Fluor 594 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... were cultured for 24h in 100 μL of RPMI medium containing 10% FCS in the presence of 5 μL of anti-CD3/CD28 beads (ThermoFisher). After centrifugation (450g ...
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bioRxiv - Physiology 2023Quote: ... and grown in DMEM/F12 with 10% FCS and penicillin–streptomycin (Life Technologies, final conc., 50 U/mL of penicillin). Differentiation was achieved by culturing the cells in differentiation media (DMEM/F12 with 2% horse serum and penicillin–streptomycin) ...
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bioRxiv - Cancer Biology 2023Quote: Freshly FACS-sorted cells were plated into 96 wells at 400,000 cells per well for each cell type and cultured for 24-48 hours in 200 ul of RPMI medium with 10% FCS (Gibco), 2 mM glutamine (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with 1:7500 goat anti-rabbit IgG A680nm and 1:7500 goat anti-mouse IgG A790nm for 1 hour at room temperature before being visualised on LiCor Odyssey Fc (Invitrogen).
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bioRxiv - Immunology 2023Quote: Surface antibody staining was conducted in PBS containing 0.1% FCS and 2mM EDTA for 30 minutes at 4°C to cells that had been pre-incubated with Fc block and LIVE/DEAD Blue (InVitrogen) staining ...
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bioRxiv - Immunology 2023Quote: ... and B16 were cultured inside tissue culture treated flasks with DMEM with 10% FCS (Hyclone) and 1X penicillin-streptomycin (100 units/ml, Gibco) at 37C and 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... All FCS measurements were carried out at ∼20 nM fluorescently labeled protein at 20°C in Nunc chambered coverslips (ThermoFisher). The chambers are pre-incubated with poly-lysine conjugated polyethylene glycol (PLL-PEG ...
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bioRxiv - Genetics 2023Quote: ... Cells were grown in DMEM with 10% FCS and penicillin–streptomycin (Life Technologies, final conc., 50 U/mL of penicillin). Differentiation into mature brown fat adipocytes was achieved by adding an adipogenic cocktail (0.5 μg/mL insulin ...
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bioRxiv - Developmental Biology 2023Quote: siRNA knockdown was performed as previously reported.20 9-12 week placental explants were placed into a 48-well plate with IMDM supplemented with 10% (v/v) FCS and gentamicin (50 μg/mL; Gibco). siRNA sequences targeting PRKCI (final concentration 0.2 μM ...
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bioRxiv - Immunology 2023Quote: Non-specific Fc binding was blocked for 10 minutes on ice with unconjugated anti-CD16/CD32 (93; Thermo-Fisher Scientific). Cells were subsequently labeled for 30 minutes on ice with the following antibodies and reagents ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with anti-CD16/32 (FC receptor block) at 1:100 and LIVE/DEAD Fixable (1:400-1:800; cat no., 15519340; Invitrogen) in 20 μl PBS for 10 min ...
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bioRxiv - Physiology 2023Quote: ... nearly 106 cells were first incubated on ice for 30 minutes in a solution consisting of a 1:10 Fc Receptor Binding Inhibitor (eBiosciences) and 1:500 Live/Dead Aqua stain (Invitrogen) diluted in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... DRGs or TGs were isolated and digested with 1 mg/ml collagenase and 2.5 mg/ml dispase dissolved in ‘basal medium’ (Neurobasal A medium supplemented with 10% FCS) (all from Gibco/Thermo Fisher) at 37°C for ca ...
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bioRxiv - Immunology 2023Quote: ... 4mM EDTA and stained for 30mins at 4°C with: Fc blocker CD16/32 (clone 93, Invitrogen #14-0161-85), CD4-AF488 (clone GK1.5 ...
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bioRxiv - Biochemistry 2023Quote: ... E14 mouse embryonic stem cells were cultured in plates coated with 1% gelatine in GMEM supplemented with 10% foetal calf serum (FCS) batch-tested for ES cell culture (Gibco), 0.1 mM non-essential amino acids ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies rabbit anti-HA (1:500) was subsequently added in permeabilization/blocking buffer (10% fetal calf serum (FCS) (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated in freshly made serum-containing medium (GMEM supplemented with 10% foetal calf serum (FCS) batch-tested for ES cell culture (Gibco), 0.1 mM non-essential amino acids ...
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bioRxiv - Immunology 2024Quote: ... BHK21 cells were purchased from ECACC (85011433) and cultured in DMEM (supplemented with 10 % FCS, 10 mM HEPES (Gibco, 15630056), 1 mM Na-Pyruvate (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% fetal calf serum (FCS; Capricorn Scientific, Ebsdorfergrund, Germany) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Dreireich, Germany) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: The binding of KIRs-Fc fusion proteins to iRBCs was assessed by flow cytometric analysis with Attune NxT (ThermoFisher Scientific). Prior to the assay ...