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Citations for Thermo Fisher :
4301 - 4350 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 2% B27 (GIBCO), 1% N2 supplement (GIBCO) ...
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bioRxiv - Microbiology 2024Quote: ... 2% B27 (Invitrogen), 1.25 mM N-acetylcysteine ...
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bioRxiv - Molecular Biology 2024Quote: ... 2-mercaptoethanol (Gibco).
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bioRxiv - Physiology 2022Quote: ... the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG; 6.83 μg/kg BW, Invitrogen) dissolved in 50% dextrose (1 g/kg BW ...
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bioRxiv - Neuroscience 2021Quote: ... the Ca+2 indicator Fluo-4-AM (Molecular Probes, Eugene, OR; 2–5 µl of 2 mM dye) were dropped over S1 cortex ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated for 30min at 37°C with 150µM of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (ThermoFisher).
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bioRxiv - Plant Biology 2020Quote: ... N121 and ΔN121 was cloned into pFast-FH vector (inserting a FLAG tag into pFast-HTB, Life Technologies, Cat. #10712-024), respectively ...
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bioRxiv - Biophysics 2021Quote: HEK293F cells grown in a 6-well plate were transiently transfected with CFTR constructs labelled with C-terminal eGFP tag using Lipofectamine 3000 (Thermo Fisher) in Opti-MEM (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... Precleared nuclear protein lysates (500 μg) were incubated with 20 μl of anti-c-MYC tag antibody coupled to magnetic beads (Thermo Scientific). After two hours ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated over night at 4°C with primary antibody mouse IgG2a anti-V5 tag monoclonal antibody (1/1000; Thermo Fisher Scientific Cat# R960-25 ...
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bioRxiv - Immunology 2021Quote: SPRR genes containing a C-terminal 6xHis tag were cloned into a pFastBac1 vector and heterologously expressed in Sf9 cells (Thermo Fisher). One liter of cells (2.5×106 cells/mL ...
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bioRxiv - Molecular Biology 2022Quote: The synthetic sequence of the 3 x FLAG tag (Table S1) was inserted between the KpnI and NotI sites of the vector pcDNA5/FRT/TO (ThermoFisher Scientific) to prepare a DNA construct for N-terminal FLAG fusions ...
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bioRxiv - Biochemistry 2022Quote: ... The cell suspension was transferred to a 96-well V-bottom plate in duplicate and fluorescently labeled with the anti-FLAG epitope tag antibody and a goat anti- mouse IgG secondary antibody conjugated with Alexa Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant baculovirus containing the sequence of ΔPRD-dynamin 1 with an N-terminal His-tag was generated by following Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). The suspension cultures of Sf9 were maintained in Sf-900 III serum-free media (SFM ...
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bioRxiv - Cell Biology 2022Quote: ... and U-2 OS Flp-In TREx CalR-HaloTag7-Pro30-SNAP-tag-KDEL cells were cultured in high-glucose phenol red free DMEM (Life Technologies) medium supplemented with GlutaMAX (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... the tryptic peptides from top and bottom of Stentor cell were labeled isobarically with the 6-plexed tandem mass tag (TMT) reagents (ThermoFisher Scientific): TMT126 (top) ...
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bioRxiv - Cancer Biology 2019Quote: KLK4-KLKP1 cDNA was PCR amplified using a forward primer with DDK tag and a reverse primer from KLK4-KLKP1 template and was cloned into Gateway expression system (Life Technologies). To generate lentiviral and adenoviral constructs ...
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bioRxiv - Plant Biology 2019Quote: In vitro pull-down assays were performed according to the manufacture’s protocol using the Pierce® HA Tag IP/Co-IP Kit (Thermo Scientific). Briefly ...
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bioRxiv - Biochemistry 2019Quote: ... variants of interest were engineered to contain a C-terminal hexahistidine tag to enable purification from conditioned media using HisPur Cobalt Resin (ThermoFisher Scientific). Sample purity was verified by the observation of only a single band on an SDS-PAGE gel following purification.
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bioRxiv - Systems Biology 2019Quote: ... The Gateway cloning cassette was introduced immediately after the SNAP tag sequence using the Gateway vector conversion system (Invitrogen, # 11828-029). All subsequent cloning steps were performed using Gateway BP and LR reactions according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... 5 muscle samples 100 μg each corresponding to 5 different age groups and one separately prepared master reference sample were labeled with 6-plex tandem mass spectrometry tags using a standard TMT labeling protocol (Thermo Fisher). 200 femtomole of bacterial beta-galactosidase digest (SCIEX ...
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bioRxiv - Microbiology 2019Quote: ... All NS3 fragments with FLAG-tag that included AA mutations and NS3 cDNA vector were digested with enzyme sets KpnI and XbaI (Thermo Scientific). Following the digestions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... NURF-1.D isoform with FLAG tag was detected using 1:1000 PIERCE ANTI-DYKDDDDK antibody (Life Technologies, Catalog number: MA191878) and NURF-1.F isoform with FLAG tag was detected using 1:1000 Millipore ANTI-FLAG antibody (Millipore Sigma ...
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bioRxiv - Immunology 2021Quote: ... containing additional S383C and D985C substitutions (Henderson et al., 2020) with a C-terminal TwinStrep tag into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Cell Biology 2021Quote: ... The coding sequence of human PLIN2 with an N-terminal EGFP tag was provided by John McLauchlan (Targett-Adams et al., 2003) and re-cloned into pCR8 (ThermoFisher Scientific). The human PLIN3 (TIP47 ...
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bioRxiv - Microbiology 2020Quote: ... Cell culture supernatant was collected after four days and purified over a 5 mL C-tag affinity matrix (Thermo Fisher Scientific). Elution fractions were concentrated and injected on a Superose 6 Increase 10/300 GL column with 1x PBS pH 7.4 as running buffer.
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bioRxiv - Biophysics 2020Quote: ... and unprocessed H57-scFV (still containing a 12x histidine tag) were removed by a batch purification with Ni-NTA agarose (ThermoFischer Scientific) and glutathione agarose (ThermoFisher Scientific). The supernatant containing biotinylated H57-scFV was further purified via gel filtration using Superdex 75 (10/300 GL ...
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bioRxiv - Molecular Biology 2021Quote: Sample aliquots comprising 25 μg of peptides were labelled with isobaric tandem mass tags (TMT 10-plex, Thermo Fisher Scientific, #90110) as described previously (31) ...
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bioRxiv - Cell Biology 2021Quote: ... and 100 μg of the total proteins from each cell line were labelled with 6-plex isobaric tandem mass tag (TMT6) reagents (Thermo Scientific) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... which then were cross-linked to polymerizing PAA gels premixes by their biotin tags to streptavidin-conjugated polyacrylamide (Streptavidin-acrylamide, Thermo Fisher). For that 7-10 μL of PAA premix of desired G’ was polymerized in the “sandwich” fashion between an “intermediate” patterned surface and glass-bottom 35 mm Petri dishes (MatTek Corp. ...
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bioRxiv - Cell Biology 2020Quote: The full-length bovine PLCγ1 with an N-terminal His10 tag and a C-terminal SNAP tag was expressed in SF9 cells using the Bac-to-Bac baculovirus expression system (Life Technologies). Cells were harvested by centrifugation and lysed by Dounce homogenizer in 50 mM HEPES (pH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... Expression vectors containing the promoter-gene-fluorescent tag cassette or 3’UTR were obtained by using LR clonase-based three-fragment recombination system (Invitrogen®), the pB7m34GW/pH7m34GW/pK7m34GW/pS7m43GW/ pLOK180_pR7m34g (gift from L ...
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bioRxiv - Cell Biology 2021Quote: ... and processed independently according to the manufacturer’s protocol for a 10-plex Tandem Mass Tag (TMT) reagent labeling kit (Thermo Fisher Scientific). The different TMT-labeled peptide mixtures were pooled equally ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples in the amount 50-100 micrograms (GFP) or 200-400 ug (HA tag) were separated using NuPAGE 4 – 12% precast gels (Life Technologies) in SDS-containing buffer and transferred to Immobilon membrane (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: The CPSF6 gene and the MBP tag were amplified by PCR and subcloned into the pcDNA3.1(+) mammalian expression vector (Thermo Fisher Scientific) using the NEBuilder HiFi Assembly kit (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... Serum supernatant was collected as described above and incubated with a pellet of 900 μl Dynabeads™ His-Tag Isolation & Pulldown (Invitrogen) equilibrated in wash buffer (50 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... both preceded by a murine IgK leader peptide and followed by an 8xHis Tag were synthetized and cloned into pcDNA™3.1/Zeo(+) expression vector (Thermo Fisher). Proteins were produced by transient co-transfection of exponentially growing Freestyle™ 293-F suspension cells (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... as measured on protein level (BCA protein concentration determination kit; before digestion) was labeled with 10-plexing tandem mass tags (TMT-10; Thermo Scientific) following the manufactures instructions ...
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bioRxiv - Microbiology 2019Quote: ... live infected cells were stained with the TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit (Invitrogen, ThermoFisher Scientific, UK), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... live infected cells were stained with the TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit (Invitrogen, ThermoFisher Scientific, UK), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... The pcDNA5/FRT-PRPF4 plasmid was constructed by cloning the PRPF4 gene fused to an N-terminal FLAG/HA tag into the pcDNA5/FRT plasmid (Thermo Fisher).
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bioRxiv - Cancer Biology 2021Quote: ... 1ul of DNA eluate isolated from the transfected samples, and 10ul of DreamTag PCR Master Mix (2X) (MgCl2, dNTPs, Dream Tag buffer and DNA polymerase) (Thermo Fisher) were used for 20ul PCR reaction on both the test sample and positive control ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... the peptides from each sample were reacted for 1 h with the appropriate tandem mass tag (TMT) isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: Insect or bacterially expressed proteins were purified by His-tag purification using hiTRAP FF columns (GE) or HisPur Resin (Thermo Scientific), followed by His tag removal by Tobacco Etch Virus (TEV ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned in frame with a N-terminal GFP tag and intervening short peptide linker (GSGEGRG) into the pcDNA5/FRT/TO bacterial expression vector (Invitrogen; V652020). A C-terminal histidine tag (Hisx6 ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant proteins containing a hexa-histidine tag was purified by passing through the nickel-NTA-affinity Column (HisPurTM Ni-NTA Spin Columns, Thermo Fisher) following supplier’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: 3T3 cells were expanded after selection to create a stable line and collected for HA-tag Co-IP analysis (Thermo Scientific™ Pierce™ HA-Tag IP/Co-IP Kit ...
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bioRxiv - Immunology 2020Quote: ... The lysates were then resolved by SDS-PAGE and immunoblotted with indicated antibodies (Wapl and V5-tag antibodies were purchased from Thermo Fisher Scientific #PA5-38024 and #R960-25 ...
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bioRxiv - Molecular Biology 2020Quote: Sample aliquots comprising 25 μg of peptides were labelled with isobaric tandem mass tags (TMT 10-plex, Thermo Fisher Scientific, 90110) as described previously31 ...