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Citations for Thermo Fisher :
4451 - 4500 of 5129 citations for TAGAP Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... A 2 μg aliquot of the peptide mixture were analysed using a LTQ-Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: The precipitated pellets were processed as described in the proteomics section and peptides were cleaned up and stored on C18 HyperSEP cartridges at −80°C until use (Thermo Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Plant Biology 2024Quote: ... A total of 1 μg of re-suspended peptide was injected per replicate using and Easy-nLC 1200 system (LC140; Thermo Scientific) mounted with an Acclaim PepMap 100 C18 trap column (Cat# 164750 ...
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bioRxiv - Cell Biology 2024Quote: ... The two supernatants were combined and subjected to high pH fractionation using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... the separated peptide was analyzed using DIA (data independent acquisition) mass spectrometry with a Q-Exactive HF-X mass spectrometer (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2024Quote: The peptides extracted from the SCO or control samples were analyzed using a nanoLC system (Dionex UltiMate 3000, Thermo Fisher Scientific) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: Peptide samples were analysed in a Data-Independent Acquisition mode (DIA) with an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) equipped with a nano-electrospray ion source and a nano-flow LC system (Easy-nLC 1200 ...
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bioRxiv - Microbiology 2023Quote: LC MS/MS data was acquired using a Dionex UltiMate 3000 RSLCnano for peptide separation and analysed with an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Scientific) with an Acclaim PepMap RSLC analytical column (75 µm x 50 cm ...
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bioRxiv - Microbiology 2023Quote: ... the eluates obtained from competitor peptide elutions were resuspended in lysis buffer and incubated with PierceTM anti-HA agarose (Thermo Fisher) for 3 h with end-over-end rotating at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The peptides were then reconstituted in MS-grade 0.1% FA in water before determining their concentration using a Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific 23290) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... and the resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Mixed peptides were re-dissolved in 300 µl 0.1% trifluoroacetic acid and fractionated according to the instructions of Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher, Cat# 84868). Samples were eluted by gradient acetonitrile of 10.0% ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting peptides were analyzed by online nano liquid chromatography (LC)-MS/MS (UltiMate 3000 RSLCnano and Q-Exactive HF, Thermo Scientific). For this ...
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bioRxiv - Cancer Biology 2023Quote: Digested peptides were run on a Q-Exactive HF mass spectrometer coupled to an EASY-nLC II 1200 chromatography system (Thermo Scientific). Samples were loaded on a 50 cm fused silica emitter that was packed in-house with ReproSIL-Pur C18-AQ ...
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bioRxiv - Cell Biology 2023Quote: ... and about 1 µg peptide was loaded onto a 2-cm trap column and separated by a 50-cm EASY-Spray column (Thermo Scientific) heated to 55°C ...
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bioRxiv - Biochemistry 2023Quote: ... Interaction of His-fused proteins with other purified proteins (e.g. GST-PKAC) or synthetic peptides was studied by pulldown using Ni-NTA affinity resin (HisPur, Thermo scientific # 88221). GST-fused proteins were pulled down on glutathione-Sepharose beads (Amersham Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific, Cat# PV3180) used to measure PDK1 activity ...
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bioRxiv - Cancer Biology 2023Quote: Peptides were resuspended in 200 mM HEPES (pH 8.5) and a 100µg aliquot of peptides was mixed with tandem mass tags (TMT, ThermoFisher Pierce, Rockford, IL) at a label to protein ratio of 2:1 ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction was then added to a new tube and the peptides were extracted and desalted using C18 desalting columns (Thermo Scientific).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The pooled and desalted sample was fractionated using high pH reverse-phase chromatography on an XBridge peptide BEH column (130 Å, 3.5 μm, 2.1 × 150 mm, Waters) on an Ultimate 3000 HPLC system (Thermo Scientific/Dionex). Buffers A (10 mM ammonium formate in water ...
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bioRxiv - Cell Biology 2023Quote: ... samples were loaded offline onto 3-phase chromatography columns and peptides were eluted using 10 MudPIT steps into an Orbitrap Elite mass spectrometer in positive ion mode (Thermo Scientific) using an Infinity 1260 quaternary pump (Agilent).
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bioRxiv - Cancer Biology 2023Quote: ... Trypsinized peptides were then analyzed on an EASY-nLC II system connected to an Orbitrap Elite ETD hybrid mass spectrometer (Thermo Fisher) using Thermo Scientific™ Xcalibur™ Software ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 mg of peptides were separated on a reverse phase C18 column and analyzed on QExactive HF Mass Spectrometer (Thermo-Fisher Scientific) as described previously 40 ...
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bioRxiv - Biochemistry 2024Quote: ... The tryptic digest was separated through online reversed-phase chromatography using a peptide trap column Acclaim PepMap 100 C18 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Desalted peptides (100 μg) from each sample were labeled with one of the TMT-10plex reagents (0.8 mg, Thermo Fisher Scientific) according to the manufacture’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 400 µg of plasma exosome peptides (pooled from 10 different plasma exosome samples) were injected onto Dionex Ultimate 3000 LC (Thermo Scientific) coupled to ACQUITY UPLC CSH1.7 mm C18 column (2.1 × 150 mm ...
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bioRxiv - Biochemistry 2024Quote: ... was used as a reference protein database and peptide spectra matches were performed using the SEQUEST algorithm within Proteome Discoverer v.2.1 (Thermo Fisher Scientific) with a fragment tolerance of 0.6 Da and parent tolerance of 10 ppm ...
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bioRxiv - Immunology 2024Quote: ... were immunized subcutaneously at two sites on the back flank area with 200 μg of MOG35–55 (MEVGWYRSPFSRVVHLYRNGK) peptide (Thermo Fisher) emulsified in 200 μl incomplete Freund’s adjuvant (BD bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... LC-MS analyses of peptide samples were performed on a hybrid ion trap- Orbitrap mass spectrometer (LTQ-Orbitrap Velos, Thermo Scientific) coupled with nanoflow reversed- phase liquid chromatography (EASY-nLC 1200 ...
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bioRxiv - Microbiology 2024Quote: ... For LC-MS/MS measurements 800 ng of peptides were analyzed on an LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLCTM 1000 UHPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... at a trypsin:protein ratio of 1:20 and peptides were purified using C18 tips (Pierce C18 Tips, 100-μl bed; Thermo Fisher Scientific). Purified peptides were diluted to 0.1 μg μl−1 and 20 μl (2 μg ...
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bioRxiv - Cell Biology 2024Quote: ... peptide samples were then dried in a speedvac and resuspended in a solution of 3% acetonitrile and 0.1% TFA and desalted using Pierce Peptide Desalting Spin Columns (Thermo Scientific # 89851). Eluants were dried in a speedvac and resusupended in a solution of 3% acetonitrile and 0.1% formic acid to a final concentration of 0.5 µg/µL ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of peptide mixtures (1µg) were loaded onto an Acclaim PepMap precolumn (75 µm x 2 cm, 3 µm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap RSLC C18 Easy-Spray column (75 µm x 50 cm ...
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bioRxiv - Cancer Biology 2024Quote: 50 µg of the of the resulting peptides after tryptic digest as described above were subsequently labeled using TMT-10plex reagents (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18, cartridge, 300 µm i.d.×5 mm, 5 µm) (Thermo Scientific), and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID ...
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bioRxiv - Molecular Biology 2024Quote: ... 1/10 of peptide solution was injected in a nanoRSLC-Q Exactive PLUS (RSLC Ultimate 3000) (Thermo Scientific,Waltham MA, USA). Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18 ...
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bioRxiv - Cancer Biology 2024Quote: ... About 5 µg digested peptides were aliquoted for global proteomics analysis by stage tip desalting and dried using Speed-Vac (Thermo Scientific). 1 µg peptides were loaded on Evotip (Evosep Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... Separated peptides were eluted directly from the column and sprayed into an Orbitrap Velos Mass Spectrometer (ThermoFisher Scientific, Hemel Hempstead, UK) using an electrospray capillary voltage of 2.7 kV ...
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bioRxiv - Cell Biology 2024Quote: LC-MS/SM analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, 100Å, Thermo Scientific) before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were eluted for 35 minutes onto a 0.075 mm × 500 mm Acclaim™ PepMap™ RSLC resolving column (Thermo Scientific) using a liner gradient ranging from 5-28% of buffer B (80% Acetonitrile/0.1% Formic Acid/19.9% Water ...
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bioRxiv - Neuroscience 2024Quote: ... Oligomers of Aβ42 were prepared by diluting the peptide solution in phenol red-free F12 medium (Thermo Fisher, Waltham MA, USA) to a final concentration of 100 μM and incubated for 24 h at 4°C followed by immediate use or storage at -80°C ...
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bioRxiv - Plant Biology 2024Quote: ... 5 µl of this peptide mix was injected onto an Acclaim PepMap RSLC HPLC column (75 µm × 15 cm, Thermo Scientific) connected to the EASY-nLC 1000 system (Thermo Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... A one microgram equivalent of peptide was subjected to LCMS/MS analysis on Orbitrap Q-Exactive Plus mass spectrometer (Thermo Scientific) in conjunction with Dionex UltiMate3000 Ultra High-Performance Liquid Chromatography (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... and were subjected to separation on an XBridge Peptide BEH C18 column (Waters) connected to an Accela high-performance liquid chromatography (HPLC) system (Thermo Scientific). A 50-min gradient of buffer B (90% ACN ...
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bioRxiv - Biochemistry 2024Quote: ... The peptide samples were loaded onto an Acclaim PepMapTM 100 C18 trap column (100 µm x 2 cm, 100 A; Thermo Scientific) and resolved on a PepMap RSLC C18 analytical column (2µm ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptide concentration was calculated based on the peak area of 100 ng of Pierce HeLa protein digestion standard (Thermo Fisher Scientific). Afterwards the peptide solution was frozen at −70°C before further processing.
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bioRxiv - Bioengineering 2024Quote: ... to peptides detected using DECODE was performed as follows: 12.5µL per well of 4 mM peptide solutions were coated on 384 well ELISA plates™ (Thermo Fisher Scientific) by shaking at r.t ...
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bioRxiv - Biochemistry 2024Quote: ... cell lysates were spiked with a known concentration of synthetic stable isotope-labeled peptides (Gerber et al, 2003) (AQUA) obtained from Life Technologies: ATPGHTG[C]LSPG[C]PDQPA(R ...