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Citations for Thermo Fisher :
4351 - 4400 of 5129 citations for TAGAP Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 25 μg of peptide labeled with TMT reagent (0.4 mg, dissolved in 40 μl anhydrous acetonitrile, Thermo Fisher Scientific, Cat # 90111) and made at a final concentration of 30% (v/v ...
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bioRxiv - Physiology 2023Quote: ... An aliquot (10%) was removed for quantification using the Pierce Quantitative Fluorometric Peptide Assay kit (Thermo Fisher Scientific, Cat. No. 23290). The remainder of the peptides from each IP samples (∼0.5-3.5 µg ...
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bioRxiv - Plant Biology 2023Quote: ... A total of 2 μg of re-suspended peptide was injected per replicate using Easy-nLC 1200 system (LC140; Thermo Scientific) and an Acclaim PepMap 100 C18 trap column (Cat# 164750 ...
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bioRxiv - Neuroscience 2023Quote: ... Eluted peptides were lyophilized and reconstituted in 300 µL 0.2% TFA for fractionation using the Pierce High pH Reversed-Phase Peptide Fractionation Kit following the instructions provided by Thermo Fisher Scientific for TMT-labelled samples ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Molecular Biology 2022Quote: Peptides were analysed by reversed-phase liquid chromatography on an EASY-nLC 1000 or 1200 system (Thermo Fisher Scientific, Odense, Denmark) coupled to a Q Exactive plus or HF mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: The prepared tryptic peptide sample of each subject was injected individually into an Ultimate3000 Nano/Capillary LC System (Thermo Scientific, UK) coupled to a Hybrid quadrupole Q-Tof impact II™ (Bruker Daltonics ...
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bioRxiv - Immunology 2022Quote: EAE was induced in 8-12 week old mice by subcutaneous injection with 100 μL of inoculant containing 100μg myelin oligodendrocyte glycoprotein peptide fragment 35-55 (MOG35-55, Thermo Scientific J66557MCR) in PBS with complete Freund’s adjuvant (CFA ...
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bioRxiv - Molecular Biology 2022Quote: ... The dried peptides were re-dissolved with 0.1% FA solution for LC-MS analysis (Q-Exactive, Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Plant Biology 2022Quote: ... Five microliters of peptide mixtures were analyzed in duplicate on a Q-Exactive Plus hybrid quadripole-orbitrap mass spectrometer (Thermo Fisher) as described in Pérez-Pérez et al ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were separated on an Ultimate 3000 RSLCnano system in-line connected to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) with a linear gradient reaching 55% MS solvent B after 85 min ...
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bioRxiv - Molecular Biology 2022Quote: ... TMT labeled peptides were analyzed by nano-ultrahigh performance (UHP)LC–MS/MS (Easy-nLC1200, Orbitrap Fusion LumosTribrid, Thermo Fisher Scientific). Proteome Discoverer 2.2 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Peptides were extracted from brains from 5 d old male flies and analyzed on an Orbitrap Fusion mass spectrometer (Thermo Scientific) equipped with a PicoView ion source (New Objective ...
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bioRxiv - Biochemistry 2024Quote: Fractionated crosslinked peptides were loaded onto a Vanquish Neo HPLC coupled to a nano-ESI source of an Orbitrap Eclipse (ThermoFisher Scientific). Samples were injected onto a 300μm x 5mm PepMap Neo Trap Cartridge peptide trap column (C18 ...
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bioRxiv - Biochemistry 2024Quote: ... The digestion was quenched to 0.5% trifluoracetic acid and spiked with Pierce Retention Time Calibrant (PRTC) peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/µL ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto an Acclaim Pepmap C18 5 µm-particle-size trap column (0.1 × 20 mm, Thermo Scientific #164564-CMD) and separated over a nanoEase M/Z Peptide BEH C18 1.7 µm-particle-size analytical column (0.075 × 250 mm ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto an Acclaim Pepmap C18 5 µm-particle-size trap column (0.1 × 20 mm, Thermo Scientific #164564-CMD) and separated over a nanoEase M/Z Peptide BEH C18 1.7 µm-particle-size analytical column (0.075 × 250 mm ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were collected by centrifugation and labelled using tandem mass tag (TMT) 11-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: 50 µg of the peptides resulting after tryptic digest as described above were subsequently labeled using TMT-10plex reagents (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Elution was performed in 30μl of wash buffer plus 5μl of 1mg/ml 3xFLAG peptide solution (Pierce™ 3x DYKDDDDK, Thermo Scientific, A36805). Samples were incubated on ice for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides (100 ng) were separated using Acclaim PepMap C18 columns (2 µm, 100 Å, 75 mm, 15 cm; Thermo Fisher Scientific). The peptides were loaded in buffer A (5% acetonitrile and 0.1% formic acid ...
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bioRxiv - Microbiology 2024Quote: A volume of 6 µl phosphopeptides or 500 ng of peptides were directly loaded onto reversed-phase pre-column (Acclaim PepMap 100, Thermo Scientific) and eluted in backflush mode ...
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bioRxiv - Molecular Biology 2024Quote: ... that were spiked with 18 pmol shTMTpro-labeled AS192 triggers were fractionated using the Pierce high pH reversed phase peptide fractionation kit (Thermo Scientific). A total of eight fractions were generated ...
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bioRxiv - Molecular Biology 2024Quote: Fractionated peptides were resuspended in 0.05% formic acid and separated using an UltiMate 3000 RSLC nano-LC system (Thermo Fisher Scientific) equipped with an analytical column (Waters nanoEase HSS C18 T3 ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were separated on a capillary column (0.075 mm × 500 mm, Acclaim Pepmap 100, reverse phase C18, NanoViper, Thermo Fisher Scientific) following a gradient of 2-40% buffer B in 128 min (A = 0.1 % formic acid B = 0.1% formic acid ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, ThermoFisher, US) using a 180-min gradient ...
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bioRxiv - Cell Biology 2024Quote: ... Online detection of peptide ions was by electrospray ionisation (ESI) mass spectrometry MS/MS with an Orbitrap Elite MS (Thermo Scientific). Ionisation of LC eluent was performed by interfacing the Sharp Singularity emitters (Fossil Ion Tech ...
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bioRxiv - Cell Biology 2024Quote: ... (Roberta Noberini et al., 2021) Peptide mixtures were separated by reversed-phase chromatography on an EASY-Spray column (Thermo Fisher Scientific), 25-cm long (inner diameter 75 µm ...
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bioRxiv - Immunology 2024Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Genetics 2024Quote: ... TMT-labeled peptides were fractionated into 8 fractions using the high pH reverse-phase peptide fractionation kit according to manufacturer’s instructions (Thermo Fisher Scientific) (Pierce High pH Reversed-Phase Peptide Fractionation Kit ...
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bioRxiv - Cell Biology 2024Quote: ... the digest peptides from DMSO and Chk2i conditions were respectively spiked with the synthetic lysine-labelled heavy phospho-peptide of (phos)TLC(Carbamidomethylation)GTPNYIAPEVLSK(+8Da) that was synthesized by Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were separated using an Acclaim PepMap RSLC analytical column (C18, 100 Å, 75 μm × 50 cm, Thermo Fisher Scientific, USA) and Acclaim PepMap trap column (75 μm × 2 cm ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... Equal amounts of peptide fragments from each experimental group were then labelled utilizing the TMTpro 16plex Isobaric Label Reagent (A44520, Thermo Fisher) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The synthetic peptides YNGVFQECCQAEDKGACLLPK (STD) and YNGVFQECCQA EDKacGACLLPK (STD*Kac) were analyzed using an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) equipped with an EASY-nLC 1200 system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: LC-MS/MS analysis of peptide samples was performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, Waltham, MA, USA) coupled to an EASY-nLC 1200 liquid chromatography (LC ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 300 µL of 0.1% trifluoroacetic acid and desalted using Pierce™ Peptide Desalting Spin Columns (ThermoFisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Chromatography of peptides prior to mass spectral analysis was accomplished using capillary emitter column (PepMap® C18, 3µM, 100Å, 150x0.075mm, Thermo Fisher Scientific). Protein annotations ...
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bioRxiv - Cell Biology 2023Quote: The processing of BioID raw LCMS data for peptide and protein identification and quantification was performed with Proteome Discoverer v2.3 (Thermo Fisher Scientific). Raw mass spectra were filtered ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were then separated with an Acclaim PepMap RSLC column (75 µm ξ 25 cm, C18 2 µm 100Å, Thermo Scientific) at 0.25 µL/min with a gradient of 4-31% solvent B (80% I ...
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bioRxiv - Biochemistry 2023Quote: ... The concentration of amino acids and unique peptides was measured using an ultrahigh-performance liquid chromatograph tandem triple quadrupole mass spectrometer (Vanquish UHPLC-TSQ Altis MS, ThermoFisher, USA). To confirm the peptide structure ...
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bioRxiv - Developmental Biology 2023Quote: ... The resultant peptides were analyzed on an Advance UHPLC system (ABRME1ichrom Bioscience) connected to a Q Exactive mass spectrometer (Thermo Fisher) processing the raw mass spectrum using Xcalibur (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... TFEB tryptic peptides were dried and reconstituted in LC-MS mobile phase A (2% acetonitrile, Thermo Scientific, cat. no.047138.M6), 98% MilliQ purified water ...
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bioRxiv - Cell Biology 2023Quote: MS analysis of TMT-labelled peptides was performed on a Q-exactive-HF mass spectrometer coupled with a Dionex Ultimate 3000RS (ThermoFisher Scientific). The detailed LC-MS protocol can be found in the supplemental materials ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were redissolved in 100mM TEAB buffer and equal amounts of peptides from each sample were labelled with TMT 10-plex Mass Tag labelling kit (#90110, ThermoFisher Scientific). After labelling ...
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bioRxiv - Cell Biology 2023Quote: The processing of whole-cell protein quantitation raw LCMS data for peptide and protein identification and quantification was performed with Proteome Discoverer (PD v3.0 (Thermo Fisher Scientific) using a SPS MS3 reporter ion-based quantification workflow ...
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bioRxiv - Neuroscience 2023Quote: ... The quantification of Aβ40 and Aβ42 peptides present in the cell culture medium was performed utilizing the Amyloid beta 40 Human ELISA Kit (Thermo Fisher Scientific) and the Amyloid beta 42 Human ELISA Kit ...
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bioRxiv - Systems Biology 2023Quote: Peptide digests of purified RSVF and antibodies were analyzed in DIA mode on a Thermo Scientific Lumos mass spectrometer (Thermo Fisher) equipped with a nanoelectrospray ion source and coupled to an Easy-nLC 1200 system (Thermo Fisher) ...
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bioRxiv - Pathology 2023Quote: ... internal diameter 100 μm) for desalting and a C18 analytical column (length 10 cm, internal diameter 75 μm) for peptide separation (Thermo Scientific). Peptides were eluted with a three-step 45 min gradient of 0.1% formic acid in ACN at a flow rate of 300 nl/min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the labeled peptides of each group were mixed in equal amounts and fractionated by a high pH reversed-phase peptide fractionation kit (Thermo Scientific). The column was first balanced by acetonitrile and 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2023Quote: ... The digested peptides were cleaned-up using the HiPPR detergent removal resin kit (PN 88305, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...