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Citations for Thermo Fisher :
4451 - 4500 of 5181 citations for S100A12 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cells were washed once and resuspended in phenol-free RPMI with 2% (v/v) human AB serum containing DAPI (ThermoFisher 62248). Single-color controls were performed with these same antibodies using BD CompBeads (BD Biosciences 552843) ...
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bioRxiv - Neuroscience 2021Quote: ... From live single cells with top 30% protein expression (evaluated by EGFP signal) the MFI of the Alexa Fluor 647-conjugated goat anti-human IgG (1:400, Life Technologies) was calculated.
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bioRxiv - Cell Biology 2021Quote: The expression cassette consisting of a Kozak sequence followed by the coding sequence for human diphtheria toxin receptor (hDTR; UniProt Q53H93) was codon-usage optimized for expression in mice and synthesized (Thermo Fisher Scientific/GeneArt ...
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bioRxiv - Cell Biology 2021Quote: ... Induced pluripotent stem cells were derived from human fibroblasts using Sendai virus-based reprogramming kit (CytoTune-iPS Sendai Reprogramming Kit; cat #. A13780-01; Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (beads/cell ratio of 1/2; Gibco, Thermo Fisher Scientific) or with 4.5 μg/mL phytohemagglutinin (PHA ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (beads/cell ratio of 1/2; Gibco, Thermo Fisher Scientific) or with 4.5 μg/mL phytohemagglutinin (PHA ...
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bioRxiv - Microbiology 2020Quote: ... human epithelial cell line were cultured at 37°C with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Invitrogen, Waltham, MA) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was extracted and the whole transcriptome of ALDH+ and ALDH-populations was assessed using Affymetrix Whole-Transcript Human Gene 1.0 ST Array (Affymetrix, Thermo Fisher Scientific) (30) ...
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bioRxiv - Neuroscience 2022Quote: Healthy wild type control (WT) iPSCs derived were obtained from Thermo-Fisher Scientific (WT, GIBCO human episomal iPSC line, cat # A18945), which were generated via reprogramming of cord blood derived CD34+ progenitors ...
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bioRxiv - Cancer Biology 2022Quote: Total RNAs were extracted from the human prostate tumors and the normal tissues and various cell lines using Trizol (Life Technologies). Reverse transcription was performed using the M-MLV reverse system (Takara ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM β- Mercaptoethanol and 1 ng/ml human recombinant leukemia inhibitory factor) for 24 h and then treated with 0.1 μg/ml Colcemid (Gibco, 15210-040) for 2.5 h ...
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bioRxiv - Microbiology 2022Quote: ... Parasites were maintained in monolayer cultures of human foreskin fibroblasts (HFFs, ATCC® SCRC-1041) in Dulbecco’s Modified Eagles Medium (DMEM, GIBCO, USA) containing 10% foetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: ... FLAG-tagged human DNA2 nuclease-deficient (D277A) and helicase-deficient (K654R) mutants were generated by Phusion site-directed mutagenesis (Thermo Fisher) of wild-type DNA2-FLAG (pIF494 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5.5μg of fragmented cDNA was hybridized on Affymetrix GeneChip™ Human Transcriptome Array 2.0 (HTA2.0) chips (Invitrogen, 902162, lot no. 4364589) for 16 hrs at 45°C ...
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bioRxiv - Cancer Biology 2022Quote: ... We collected viral supernatants after 48 h and used them to infect the Jurkat human cell line by spinoculation with 4 μg ml-1 Polybrene Infection/Transfection Reagent (Fisher Scientific). We selected infected human cell lines with 1 mg ml-1 blasticidin (InvivoGen ...
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bioRxiv - Cell Biology 2022Quote: ... Transcriptional profiling was performed with the use of the Affymetrix Human Genome U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA). All labeling reactions and hybridizations were carried out according to the standard GeneChip® eukaryotic target labeling protocol ...
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bioRxiv - Cell Biology 2022Quote: ... DNAs were amplified by PCR from human Caco2 cells or MCF7 breast cancer cells and cloned into Gateway™ expression vectors (Invitrogen) according to the manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h with anti-human IgG goat polyclonal antibodies conjugated to FITC or Alexa Fluor 488 (H10301; A-11013, Invitrogen, USA) diluted 1/100 in FACS buffer ...
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bioRxiv - Genomics 2022Quote: PBMC culture supernatants were analyzed for secreted proteins with Immune Monitoring 65- Plex Human ProcartaPlex Panel (Thermo Fisher Scientific, Carlsbad, CA) as per manufacturer’s instructions (all proteins assayed shown in Table S5) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well-plates and treated with 10μg/ml OM-85 concentrate (OM-Pharma) or 100 ng/mL of human recombinant IFN-β protein (ThermoFisher Scientific). The concentration of 10μg/ml of OM-85 was based on previous data with RSV we recently generated [9] ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 2.5 µg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes [65] using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ & mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ and mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Cancer Biology 2024Quote: ... #D-001810-10-05) or human GPX3 siRNA (Dharmacon, #L-006485-00-0005) were delivered to the cells using Lipofectamine RNAiMAX (Invitrogen, 13778150). After 24 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20 ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 pmol of desthiobiotinylated HOXC13-AS was mixed with magnetic beads and incubated with 200 µg of protein lysate from human adult epidermal keratinocytes (ThermoFisher Scientific). Poly(A)25 RNA ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2/UT-NCGM02/Human/2020 (Wuhan strain) was propagated in VeroE6/TMPRSS2 cells in VP-SFM (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: Total RNA from HeLa cells (human) or melanocytes (mouse) melan-Ink4a (Shakya et al., 2018) was isolated using a GeneJET RNA purification kit (ThermoFisher Scientific) in the presence of β-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... established mature DB-VOs at day 15 from diabetic iPSCs were started to culture in high glucose concentration (33mM, adapted from our previous publication (Yang et al., 2020)) plus 1 ng/ml human TNF (Invitrogen, PHC3011) and 1 ng/ml IL-6 (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: PBMC culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel (Thermofisher Scientific, Carlsbad, CA), following previously documented procedures.43,44 This is a preconfigured multiplex immunoassay kit that measures 65 cytokines ...
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bioRxiv - Molecular Biology 2024Quote: Approximately 800,000 U2OS cells were reverse transfected with 20 nM siRNA against the 3’ UTR of endogenous TIRR or a negative control siRNA (Dharmacon ON-TARGETplus Human NUDT16L1 siRNA J-014872-23-0005 or ON-TARGETplus Non-targeting Control) using 3 uL of RNAiMAX lipofectamine (Thermo Fisher) in a 6-well plate ...
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bioRxiv - Bioengineering 2024Quote: ... The cell clumps in Matrigel dome were cultured in the EM supplemented with 25 ng/mL human active BMP7 recombinant protein (Gibco; PHC7204) initially for 3 - 6 days to regenerate and form Exp-HLOs using BMP7 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were then transfected with 25 nM of either ON-TARGETplus Human ABCB1 (5243) SMARTpool or non-targeting pool siRNAs (negative control, Dharmacon) using the Lipofectamine RNAiMAX reagents (Thermo Fisher). Following 24 hours of incubation ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary antibodies or co-stains were as follows: Alexa Fluor 647-conjugated streptavidin (surface display staining other than TfR human/mouse cross-reactivity, ThermoFisher S21374); iFluor 647-conjugated anti-His-tag antibody (pilot CYpHER detection and TfR human/mouse cross-reactivity ...
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bioRxiv - Bioengineering 2024Quote: Human fibroblast (ATCC) and SKOV-3 cells (graciously donated from the Dr. Hoshino lab) were cultured on T75 flasks (Fisher Scientific) at 5% CO2 at 37° C using McCoys 5A media (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Protein concentrations from fractions 2 and 3 of fed human milk and digesta were determined using a Micro BCATM kit (Pierce, Thermo Fisher), following manufacturer instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Ptf1aCreER;Insrf/f;nTnG acinar cells and the control cells were cultured with Human plasma-like media (Gibco, Cat. No. A4899102), instead of RPMI1640 ...
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bioRxiv - Cancer Biology 2024Quote: ... and human gastrointestinal stromal tumour cells (GIST882) (National Infrastructure of Cell Line Resource, Beijing, China) were seeded in confocal dish (ThermoFisher Scientific) and grew in DMEM in 5% CO2 in a 37 °C incubator ...
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bioRxiv - Biophysics 2024Quote: ... primary stained with goat anti-Syndecan-1 antibody and mouse anti-human choriogonadotropin (β-hCG) antibody (#14-6508-82, 1:500 dilution in the buffer solution, ThermoFisher), and secondary stained as previously described ...
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bioRxiv - Cancer Biology 2024Quote: ... and PANC-1 (CRL-1469, ATCC) and the human colorectal cancer cell line HT29 (HTB-38, ATCC) were grown in RPMI-1640 (31870025, Thermofisher Scientific), DMEM (11965092 ...
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bioRxiv - Cell Biology 2024Quote: ... human EGF (10 µg/ml) and bovine insulin (10 µg/ml) in low-attachment plates (Nunclon sphera, Thermo Scientific™ #174932) and maintained at 37°C in a humidified 5% CO2 incubator for 3 days before harvesting ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 ng/ml human recombinant epidermal growth factor (EGF) and 50 µg/ml bovine pituitary extract (BPE)(all Thermo Fisher). Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... 6-well plates were coated with 20 µg/mL Human recombinant laminin-521 (Biolaminin; Biolamina; LN521-05) in 1x dPBS++ (Gibco; 14040117) and incubated overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... T cells (1 million cells/ml) were activated with Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Fisher) in culture medium ...
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bioRxiv - Immunology 2024Quote: ... primed NK cells from PWH were cultured with autologous CD4+ T cells also isolated by negative immunomagnetic selection (DynaBeads Human CD4+ T cell isolation kit, Life technologies) in the presence of 50nM Romidepsin (Selleck Chemicals ...
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bioRxiv - Pathology 2024Quote: ... and slides were analyzed independently by 3 experienced reviewers to identify and measure the area of human cardiomyocyte grafts using an EVOS (Life Technologies) microscope ...
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bioRxiv - Cell Biology 2024Quote: Cytokine secretion of PMA treated THP-1 cells was analyzed using Cytokine & Chemokine 34-Plex Human ProcartaPlex™ Panel 1A (Invitrogen) and measured with xMAP LuminexTM system (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... Human TS cells were cultured in six-well plates precoated with 5 μg/mL of CorningTM collagen IV (CB40233, Thermo Fisher) and Basal Human TS Cell Medium [Dulbecco’s Modified Eagle Medium/F12 (DMEM/F12 ...
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bioRxiv - Genomics 2024Quote: ... and were activated for 72h using Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermofisher, 11161D) according to the manufacturer’s instructions at 37°C under 5% CO2 ...