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Citations for Thermo Fisher :
4451 - 4500 of 10000+ citations for 6 Methoxy 2 3 4 9 tetrahydro 1H b carbolin 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Adherent semiconfluent HEK293SG cells cultured in DMEM:F12 medium supplemented with 10% FBS were transfected in 6-well plates with 2 μg/well of plasmid DNA complexed with Lipofectamine 2000 reagent (Invitrogen). The cells were incubated for 48 hrs at 37 °C under 7% CO2 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in urea buffer (6 M urea, 2 M thiourea, 100 mM ammonium bicarbonate, pH 8.0) and quantified using EZQ (Invitrogen/Life Technologies).
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells at ∼50% confluency in 6-well plates were incubated with 2 mL Opti-MEM-I medium (Gibco, 31985062) supplied with 6 µL Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected in 6-well plates with 2 µg of the indicated constructs in the presence of Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI) in phosphate buffered saline (PBS) and cells were mounted with ProLong Gold Antifade reagent (Invitrogen).
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bioRxiv - Bioengineering 2024Quote: ... Hepatic endoderm specification was initiated on differentiation days 6 ─ 10 using RMPI supplemented with 2% B27 and insulin (ThermoFisher, #17504044), 10ng/ml FGF-2 ...
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bioRxiv - Neuroscience 2024Quote: ... with real time quantitative gene expression determined using using Taqman probles (Table 2) and analysed by the Quant Studio 6 Flex Real-Time PCR System (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with 4-6 μg linearized targeting vector DNA (ROSA26-SpCas9) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, USA), selected with 1400-1600 μg/mL G418 (Genaxxon Bioscience ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from liver tissues of SHR and WKY (4-6 weeks old) using the TRIzol reagent (Invitrogen, Waltham, USA) followed by purification using Nucleospin columns (Machery-Nagel ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 × 105 of isolated MLEC were cultured in a 4-well chamber slide (Nunc Lab-Tek Chamber Slide System, Thermo Fisher Scientific) precoated with Extracellular Matrix Attachment Solution provided in the kit ...
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bioRxiv - Genetics 2020Quote: MEFs were fixed on 6-well glass bottom dishes with 4% PFA (diluted from 16% stock in PBS; ThermoFisher Scientific, Massachusetts, US), washed with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were typically transfected in a 6 well plate format with 20 μM siRNAs and 4 μL Lipofectamine RNAiMAX (Thermo Fisher Scientific); the depletion phenotype was inspected 72 h after transfection unless otherwise indicated in the text or the legends ...
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bioRxiv - Cancer Biology 2022Quote: ... B16F10-TR-shApoe cells were plated into a 6-well dish the day prior to transfection and transfected with 4 μg plasmid and TurboFect transfection reagent (Thermo Scientific, R0533) diluted in serum-free media ...
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bioRxiv - Immunology 2022Quote: ... Bacteria were harvested after 16–18 h at 3000 x g for 15 min at 4°C (Sorvall LYNX 6000 centrifuge, F9-6×1000 LEX rotor, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Nuclei was then isolated using a sucrose gradient and ultracentrifugation at 13,000 rcf for 45 mins at 4°C (Sorvall WX-100, F65L-6 x 13.5 rotor, Thermo Fisher, Waltham, MA USA). Isolated nuclei were resuspended in Nuclei PURE Prep kit storage buffer and RNAase inhibitor ...
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bioRxiv - Genetics 2021Quote: ... 6-VIC ® or 6-PET® (Life Technologies Corporation, Carlsbad, USA). During capillary electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... in a 3:1 ratio with 1X penicillin/streptomycin (Gibco; 15070) and 5% FBS (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... rat Taste receptor type 1 member 3 (Tas1r3, Rn00590759_g1, Applied Biosystems) and rat Taste receptor ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... in Fibroblast medium (3:1 DMEM: Ham’s F12 Nutrient Mixture, Gibco) supplemented with 10% FCS ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were counterstained with ToPro-3 (1:1000 dilution; Life Technologies).
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bioRxiv - Cell Biology 2020Quote: ... and washed 3 times with 1x PBS (Fisher Scientific, BP399-1).
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bioRxiv - Microbiology 2020Quote: ... and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5,000 dilution, Invitrogen). Secondary antibodies against rabbit and mouse IgG conjugated to IRDye 680LT or IRDye 800CW were obtained from Li-Cor (1:10,000 dilution) ...
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bioRxiv - Cancer Biology 2022Quote: ... BxPC-3 and PSN-1 were cultured in RPMI1640 (Thermo Fisher), supplemented with 10% FCS and 1% P/S ...
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bioRxiv - Microbiology 2022Quote: ... 1 nM TO-PRO™-3 Iodide (642/661 nm, Thermofisher) and 8 ng/ml calcofluor-white (CW ...
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bioRxiv - Microbiology 2022Quote: ... 1 nM TO-PRO™-3 Iodide (642/661 nm, Thermofisher) and 8 ng/ml calcofluor-white (CW ...
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bioRxiv - Developmental Biology 2019Quote: ... Nuclei were labeled using TOPRO-3 (1:1000, T3605, Life Technologies). Brains were mounted for confocal microscopy in Vectashield (Vector Laboratories ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were collected into FAD media (DMEM/F12 3:1 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Immunology 2019Quote: ... 1 cm pieces and washed 3 times with 1x HBSS (Gibco) containing 15 mM HEPES (Thermo Scientific) ...
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bioRxiv - Genomics 2021Quote: ... for RPE1] in 1:3 ratio in Opti-MEM media (Gibco), incubated for 15 min at RT ...
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bioRxiv - Neuroscience 2022Quote: Neurons were transfected at DIV 1-3 using Lipofectamine 2000 (Invitrogen). For one Ø18-mm coverslip seeded with 100,000 neurons ...
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bioRxiv - Immunology 2021Quote: ... and goat anti-mouse Cyanine 3 (Life technologies, A10521, 1:1000). Images for BrdU staining were taken using a Zeiss Confocal (LSM710 META) ...
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bioRxiv - Microbiology 2020Quote: ... 3 μl of DNA ladder (1 Kb Plus DNA Ladder; Invitrogen), 6 μl of positive control and 10μl of template DNA were ran on 2.5% (w/v ...
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bioRxiv - Genomics 2021Quote: ... Linker oligo sequences were: 5’ – TTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNNCAGGCTACTCCGCTTAAGGGAC-3’ (linker 1, Invitrogen, UK) and 5’-GTCCCTTAAGCGGAGTAGCCTG/3AmMO/-3’ (linker 2 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μM FluoZin-3 tetrapotassium salt (Thermo Fisher Scientific, Waltham, MA) was then added to each reaction ...
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bioRxiv - Cell Biology 2022Quote: ... Incubation with TO-PRO-3 (1:100, ThermoFisher Scientific Ref. T3605) was performed along with secondary antibody incubation to stain nucleic acid ...
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bioRxiv - Immunology 2022Quote: ... 3 µl of Dynabeads MyOne Carboxylic Acid (1 μm; ThermoFisher Scientific) were added at a concentration of 0.5 mg/ml to each of the samples ...
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bioRxiv - Immunology 2024Quote: ... were coated with 3 µg ml-1 of streptavidin (Thermo Fisher) diluted in carbonate-bicarbonate buffer (E107 ...
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bioRxiv - Genomics 2022Quote: ... at a 1:3 ratio in Opti-MEM medium (Gibco, 11524456), incubated for 15 minutes at room temperature and added dropwise to the cells ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-14-3-3γ 1:2000 (Thermo fisher PA5-29690) and mouse anti-GAPDH (CSB-MA000195 ...
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bioRxiv - Microbiology 2023Quote: ... HSV-1 Probe FAM-5’-CGGCCCAACATATCGTTGACATGGC-3’-MGBNFQ (Thermo Fisher Scientific). The efficiency of each round of PCR was determined using 10-fold dilutions of Topo TA plasmids (Invitrogen AB ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was labeled with ToPro-3 (1:5000; Invitrogen, Cat #T3605) in 0.3% PBST for 30 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen; 1:1000) was used to measure apoptosis ...
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bioRxiv - Physiology 2023Quote: ... counterstained with 1:30,000 TO-PRO-3 Iodide (Life Technologies, T3605), and then mounted in SlowFade Diamond mounting medium (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... Cy-3 conjugated anti-rabbit secondary antibody (Invitrogen, 1:300 dilution) was added and incubated for 0.5 hr ...
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bioRxiv - Neuroscience 2024Quote: ... larvae were immersed in 3 µM FM 1-43FX (ThermoFisher, F25255) in E3 for 30 s ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected and diluted 1:6 for BCA protein quantification (ThermoFisher #23227) and performed according to manufacturer’s guidelines ...
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bioRxiv - Microbiology 2019Quote: ... 6×His-Lsr2 was purified by binding to 1 mL Ni-NTA agarose (Invitrogen), after which the resin was collected and the bound protein was washed with binding buffer supplemented with increasing concentrations of imidazole ...
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bioRxiv - Bioengineering 2021Quote: ... The composition of 1 L MM1 (pH = 6) was 10 g glucose (Acros Organics), 5 g of (NH4)2SO4 (Lach-Ner) ...
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bioRxiv - Genomics 2021Quote: ... and 6 μl lysozyme solution (50 mg.ml-1, Thermo Fisher Scientific, Cat. No.: 90082) and incubated for 60 min at 37°C with light mixing at 300 rpm using the Eppendorf ThermoMixer® C (Eppendorf ...