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Citations for Thermo Fisher :
4301 - 4350 of 10000+ citations for 6 Methoxy 2 3 4 9 tetrahydro 1H b carbolin 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: One hundred thousand cells per condition were fixed for 10 minutes with 1% paraformaldehyde (PFA, Thermofisher), then washed once with 4 mL of FACS buffer (2% FCS in PBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligated plasmid (1 µl) was then transformed into One Shot BL21 Star (DE3) competent cells (Invitrogen) with outgrowth in SOC media for 1 hour at 37°C before plating on LB agar plates containing kanamycin (50 µg/ml) ...
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bioRxiv - Immunology 2019Quote: ... Total serum F(ab’)2 was then applied to a one ml custom made affinity column comprised of IFX-F(ab’)2 coupled to NHS-activated agarose beads (Thermo Fisher Scientific). The purified serum F(ab’)2 were recycled three times over the affinity column ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM DTT supplemented with Pierce Protease and Phosphatase Inhibitor Mini Tablets (40 μL/mL of stock solution – one tablet / 2 mL H2O, Thermo Scientific, 88668) and 25 U/mL of benzonase (Merck-Millipore ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with Pierce Protease and Phosphatase Inhibitor Mini Tablets (40 μL/mL of stock solution – one tablet / 2 mL H2O, Thermo Scientific, 88668). Samples were kept on ice for 30 minutes and centrifuged for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... with one notable difference being that sodium azide was excluded from FACS buffer (2% FBS and 1mM EDTA [Fisher Scientific] in PBS) to ensure optimal CD138 staining (Wilmore et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with 4.5 μg of either pSpCas9(BB)-2A-GFP control plasmid DNA (Ran et al, 2013) or one of 2 different TBK1 targeting plasmids using Lipofectamine® LTX with Plus™ Reagent (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... 2 µL of one-tenth diluted RT products were amplified in 2X ABsolute qPCR SYBR Green Mix (Thermo Fisher Scientific, Illkirch, France) with 1.6 µM of specific primers with an QuantStudio™ 6 Flex system (Applied ...
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bioRxiv - Immunology 2022Quote: Preparation of CD5L-coated pHrodo particles: one vial containing 2 mg of particles (pHrodo™ Red E. coli BioParticles™ Conjugate for Phagocytosis, ThermoFisher) were reconstituted with 500 μL of TBS with 5 mM CaCL2 and sonicated for 5 min ...
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bioRxiv - Pathology 2024Quote: ... immunohistochemistry was performed using one of two rabbit polyclonal primary antibodies (COX-2, Biocare, CRM-306 and TGF-B1, Invitrogen, PA1-29032) that were incubated for 30 minutes at room temperature ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were cut into 2-4 mm3 pieces and resuspended in RPMI 1640 media supplemented with 2% FBS (Thermo Scientific, Waltham, MA, USA). These pieces then underwent mechanical and enzymatic digestion for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... 1% (w/v) penicillin-streptomycin and 0.01 mg/ml hygromycin B (all from Thermo Fisher Scientific UK). For experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Myo21 heterozygous mutants were maintained in the same medium with 10 μg ml−1 hygromycin B (Invitrogen). Episomally complemented heterozygous Myo21 mutants were maintained in the same medium with 10 μg ml−1 of hygromycin B and 10 μg ml−1 of G418 Sulfate (Sigma) ...
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bioRxiv - Genetics 2022Quote: ... supplemented with 10% fetal bovine serum (MilliporeSigma TMS-013-B) and 1% penicillin/streptomycin (ThermoFisher 15140-122). MEFs were cultured at 37 C with 5% CO2 and passaged every 2-3 days following the same passaging protocol as performed for mESCs (above) ...
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bioRxiv - Molecular Biology 2020Quote: ... in sealed 35-mm culture dishes containing 1.5mL DMEM supplemented with 1 x B-27 plus (Gibco), 352.5 μg/mL sodium bicarbonate ...
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bioRxiv - Bioengineering 2023Quote: ... 80 μM Rhodamine B dissolved in DMSO is sandwiched between two thin coverslips (No.1; Thermo Fisher) for the measurement ...
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bioRxiv - Physiology 2023Quote: ... and a 20-min incubation with TSA-555 (1:200, Thermo Fisher Scientific, Cat. No.: B-40957). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... Bladder tumor organoids were cultured in a culture medium containing Ad+++ supplemented with 1 × B-27 (Gibco), 1.25 mM N-acetylcysteine (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... cat no: B-002000-UB-100) and 2 μL (A673) or 2.5 μL (CHLA10) Liopfectamine RNAiMAX transfection reagent (Life Technologies, cat no: 13778150). The mixture was incubated with intermittent rocking for 30 minutes prior to the addition of cells.
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bioRxiv - Neuroscience 2020Quote: ... and cultured for 7 days in vitro in Neurobasal® medium supplemented with 2% v/v B-27® supplement medium and 1mM L-glutamine (all from Life Technologies) at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was mixed in a 4:1 ratio with 4-methylvaleric acid (Thermo Fisher catalogue number AAA1540506) in 6% v/v phosphoric acid and copper sulfate pentahydrate ...
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bioRxiv - Developmental Biology 2020Quote: Transfection mix was prepared by combining PEI and plasmid DNA (4:1 ratio; 4 µg PEI per 1 µg DNA) in Opti-MEM™ Reduced Serum Medium (Thermo Fisher Scientific) followed by brief vortexing ...
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bioRxiv - Immunology 2021Quote: ... were cocultured at 1:1 ratio for 6 days with anti-CD3/CD28–coated beads (Dynabeads, Life Technologies) at 1 bead ...
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bioRxiv - Cell Biology 2020Quote: ... Digested glomerular fractions were added to 9 ml RPMI (Thermo Scientific 11875119) containing 10% FBS (Thermo Scientific 16000044 ...
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bioRxiv - Genomics 2020Quote: ... were transferred by using 9 μl Lipofectamine RNAi MAX Reagent (Invitrogen, USA). Matrigel (250 μl per well ...
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bioRxiv - Cancer Biology 2019Quote: ... they were transfected with 9 μL of Lipofectamine 2000 (Thermo Fisher #11668027), 1.5 μg per well of PsPax2 (Addgene #12260) ...
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bioRxiv - Biophysics 2019Quote: ... SF-9 cells were grown in Sf-900™ III medium (Gibco) complemented with 0.5% 100× Antibiotic-Antimycotic (Gibco ...
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bioRxiv - Bioengineering 2019Quote: ... biofilms and cells are dyed using Syto-9 (Thermo Fisher Scientific S34854) and Calcein AM (Thermo Fisher Scientific L3224) ...
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bioRxiv - Microbiology 2022Quote: The pellicle biofilm was stained with 5 μM SYTO 9 (Thermo Scientific), 5 μM Propidium Iodide (PI ...
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bioRxiv - Molecular Biology 2021Quote: ... and blotted for 9 s using a Vitrobot Mark IV (ThermoFisher, Eindhoven) operated at 4°C and 100% humidity.
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bioRxiv - Cancer Biology 2021Quote: ... SCC-9 and SCC-25 cells were cultured in DMEM/F12 (Gibco) supplemented with 400 ng/mL of hydroxycortisone (H0888 ...
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bioRxiv - Cell Biology 2022Quote: ... and mixed with 9 µL Pluronic F-127 (Fisher Scientific, P-3000MP) by vortexing ...
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bioRxiv - Genomics 2022Quote: ... All transfections were performed using 9 uL of Lipofectamine 2000 (Invitrogen, 11668), 1.5 µg of luciferase vector ...
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bioRxiv - Molecular Biology 2022Quote: ... falciparum 3D7 and RKL-9 were cultured in RPMI 1640 (Invitrogen, USA) supplemented with 27.2 mg/L hypoxanthine (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... siRNA (45 nMols) and 9 μL of RNAiMax transfection reagent (ThermoFisher Scientific) were incubated in 300 μL of OptiMem media (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 9 in small tubes (Thermo Scientific, Thin-Walled WX, no.03699) and centrifuged at 32,900xg ...
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bioRxiv - Cancer Biology 2022Quote: ... All transfections were performed using 9 uL of Lipofectamine 2000 (Invitrogen, 11668), 1.5 μg of luciferase vector ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 9% foetal bovine serum (FBS) (10099, Gibco™, Thermo Fisher Scientific Inc ...
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bioRxiv - Biochemistry 2024Quote: ... were transfected with 5-9 µg of bacmid DNA using ExpiFectamine (Gibco) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... All media was supplemented with 9% fetal bovine serum (FBS) (Gibco, #26140), and all cells were grown at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: Cells expressing the eGFP-RBD3 probe [24] were grown in SD medium at 30° C until exponential phase(in the range 0.6-1 × 107 cells mL-1) and incubated with the mitochondrial marker rhodamine B hexyl ester perchlorate (Molecular Probes, Eugene, OR) 100 nM final concentration for about 5 min before imaging ...
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bioRxiv - Genetics 2023Quote: ... the medium was removed and NIM was added with doxycycline plus 5 µg ml–1 puromycin and 250 µg ml–1 hygromycin B (Thermo Fisher Scientific) (NIM selection medium) ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed again and then incubated for 1h in Alexa FluorTM 594-conjugated streptavidin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then harvested and stained for 1h at room temperature with 8 μM CCF4-AM (Invitrogen) in EM medium supplemented with 2.5 μM probenecid ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then incubated for 1h with anti-rabbit Alexa Fluor 568 secondary antibodies (Molecular Probes-Invitrogen) diluted at 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then incubated for 1h with anti-rabbit Alexa Fluor 568 secondary antibodies (Molecular Probes-Invitrogen) diluted at 1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed HUVEC were blocked for 1h at RT in blocking solution (5% FBS, 2X antibiotic-antimycotic (Gibco), 0.1% sodium azide (s2002-100G ...
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bioRxiv - Molecular Biology 2023Quote: ... Separated proteins were transferred to nitrocellulose membrane (Amersham) 1h at 120V using 1x NuPAGE Transfer Buffer (Invitrogen) supplemented with 10% methanol ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody was detected after probing for 1h with HRP-linked rabbit anti-mouse IgG (AB_228307, Thermofisher) or goat anti-rabbit IgG (AB_228341 ...