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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Zika Virus Envelope Protein Suriname strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Vero cells were used to propagate the virus in DMEM medium (Gibco) supplemented with 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Virus was removed from cells and replaced with post-infection media (Gibco OptiMem supplemented with 0.01% FBS ...
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bioRxiv - Biophysics 2022Quote: ... X-31 influenza virus was diluted in Opti-MEM (Gibco, cat # 11058021) at a ratio of 1:10000 to add 250 µL into each well (25) ...
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bioRxiv - Microbiology 2022Quote: ... the reactions with Taqman Fast Virus 1-step master mix (ThermoFisher scientific) were half of the recommended 20 μl volume ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-containing pellet was resuspended in 200 μL of DPBS (ThermoFisher) and stored at -80 °C ...
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bioRxiv - Microbiology 2023Quote: ... 10-fold dilutions of each virus in DMEM + 1% antibiotic/antimycotic (Gibco) were added to a monolayer Vero E6-TMPRSS2-T2A-ACE2 cells for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis was performed with Moloney murine leukemia virus reverse transcriptase (Invitrogen) at 38°C for 60 min ...
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bioRxiv - Microbiology 2023Quote: ... Each virus was mixed with PBS-washed defibrinated sheep blood (Fisher Scientific) containing 5 mM ATP to a concentration of 106 PFU/mL and fed to ∼50-60 female mosquitoes ...
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bioRxiv - Microbiology 2021Quote: Plasmodium falciparum strain 3D7 was cultured in RPMI media (Invitrogen) supplemented with 0.5% (w/v ...
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bioRxiv - Developmental Biology 2022Quote: ... coli strain BL21 and purified on glutathione-agarose (Thermo Scientific). Purified protein was used to immunize guinea pigs (Cocalico Biologicals).
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... falciparum strain NF54attB (15) was cultured in RPMI 1640 (Gibco) supplemented with 0.25% (w/v ...
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bioRxiv - Developmental Biology 2019Quote: ... coli strain BL21 and purified on glutathione-agarose (Thermo Scientific). Purified proteins were used to immunize guinea pigs (Cocalico Biologicals ...
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bioRxiv - Microbiology 2019Quote: MRSA strains were grown overnight in bicarbonate-free DMEM (Gibco) supplemented with 25 mM NaHCO3 and diluted into the same medium supplemented with 150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... major strains using TRIzol reagent (Invitrogen, Carlsbad, California, United States). DNA contamination was further removed with DNaseI (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... pastoris strain X33 was purchased from Invitrogen (Cergy-Pontoise, France). The nucleotide sequence of LbGH28A without its signal peptide sequence (Supplemental Information Figure S1 ...
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bioRxiv - Plant Biology 2020Quote: ... were introduced into the Saccharomyces cerevisiae strain INVSc1 (Thermo Fisher). The empty pYES-DEST52 vector was transformed as a negative control ...
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bioRxiv - Microbiology 2022Quote: ... coli strains were cultured in LB broth (Thermo Fisher Scientific) supplemented with 100 mg/L ampicillin (FUJIFILM Wako Pure Chemical Corporation ...
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bioRxiv - Molecular Biology 2021Quote: ... The hsp42-GFP (1005) yeast strain was purchased from Invitrogen. The Hsp42-expressing strain on the BY4741 (1178 ...
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bioRxiv - Biochemistry 2021Quote: ... coli Bl21 (λDE3) and DH5α strains were supplied by ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... expression in Escherichia coli strain BL21 Star DE3 PlysS (Invitrogen), and purification from inclusion bodies were performed as previously described [33 ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain BL21 (de3) pLysS (C606003, Thermo Fisher Scientific, USA). GST-fused LRRC23 recombinant protein was expressed and subjected to the treatment of PreScission Protease to remove the GST tag [41] ...
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bioRxiv - Microbiology 2022Quote: ... and then transformed into PichiaPink™ Strain one (Invitrogen, USA) by electroporation as per the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... and then transformed into PichiaPink™ Strain one (Invitrogen, USA) by electroporation as per the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2022Quote: ... cerevisiae haploid strain BY4741 Arl3-GFP was obtained from Invitrogen. Deletion of NAA30/MAK3/YPR051W was performed by homologous recombination as previously described and verified by PCR (Osberg et al. ...
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bioRxiv - Plant Biology 2022Quote: ... strain GS115 using the EasySelect™ Pichia Expression Kit (Invitrogen). Transformants were selected on plates containing 100 ...
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bioRxiv - Cell Biology 2023Quote: ... coli strains used in this study include Mach1T1R (Thermo Fisher), BL21 (DE3)T1R (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... coli strains used in this study include Mach1T1R (Thermo Fisher) and BL21 (DE3)T1R (Sigma) ...
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bioRxiv - Microbiology 2024Quote: Glycerol stock of Escherichia coli (E. coli) (TOP10 strain, Invitrogen, Thermo Fisher Science ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5 μg pCMV-VSV-G envelope and 0.5 μg pGP packaging plasmids using Lipofectamine 3000 transfection reagent (Invitrogen, Carlsbad, CA, US) according to the manufacturer’s recommendation ...
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bioRxiv - Immunology 2021Quote: ... the PSG3 plasmid was co-transfected into HEK293T cells along with JRFL or YU2 HIV envelope plasmids using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...
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bioRxiv - Cancer Biology 2022Quote: ... Transient retroviral supernatants were generated by transfecting the vector encoding the TCR of interest (MSGV1) and envelope (RD114) into 293GP cells using Lipofectamine 2000 (Life Technologies). PBLs were activated in T cell medium supplemented with 50 ng/mL anti-CD3 and 300 IU/mL IL-2 for 3 days before retroviral transduction ...
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bioRxiv - Molecular Biology 2019Quote: Genomic DNA was extracted from the PhaZ-producing strains (RNA/DNA purification kit, Norgen Biotek for C. testosteroni, and GeneJET Genomic DNA Purification Kit, ThermoFisher Scientific for other strains). Inserts were obtained by amplifications of the mature phaZ genes (without signal peptides ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was harvested for virus titration and cells were lysed in Trizol (Invitrogen). MHV in the supernatant was quantified by standard plaque assay on L2 cells.
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bioRxiv - Cell Biology 2020Quote: ... HEK-293T cells used for virus production were cultured in IMDM (Life Technologies) supplemented with 20% IFS ...
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bioRxiv - Neuroscience 2020Quote: ... Virus-containing supernatant was harvested three days after transfection with Lipofectamine 2000 (Thermofisher). This supernatant was then cleared from cell debris by centrifugation at 3500 rpm for 15 min and filtered through a 0.45 µm filter (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... to wash the virus in Opti-MEM™ Medium (ThermoFisher Scientific, Darmstadt, Germany) and concentrate to a final volume of 150 μl.
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bioRxiv - Neuroscience 2020Quote: ... They were reprogrammed into iPSCs using the replication incompetent Sendaï virus kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The virus pellet was then re-suspended in PBS (Thermo Fisher Scientific, 10010015) containing 25 mM HEPES buffer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... For this the virus was diluted in 3 mL of 199 medium (Gibco). After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... respectively) and 5 μl of 4X TaqMan Fast Virus 1-step mastermix (ThermoFisher). The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... using the Taqman Fast Virus 1-stepMaster Mix enzyme (Thermo Fisher, Waltham, MA). The limit of detection of this assay is estimated to be 200 genome equivalents/ml saliva or swab fluid ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
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bioRxiv - Microbiology 2021Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 1X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific; 4444434) for a total reaction volume of 20 μl in 96-well PCR plates (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) in a total reaction volume of 10 μl ...
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... ten-fold dilutions of virus were prepared in diluent medium (Opti-MEM (Invitrogen) with 2% FBS (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-Rex 293 HEK cells (Invitrogen) which repress antigen expression during virus propagation ...
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bioRxiv - Immunology 2021Quote: ... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...