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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Zika Virus Envelope Protein Suriname strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... hiPSCs were generated from primary fibroblasts using Sendai virus reprogramming kit (ThermoFisher Scientific), yielding 2-3 different clonal lines for each individual ...
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bioRxiv - Cancer Biology 2022Quote: ... packaging cell line for virus production were cultivated in DMEM high glucose (Gibco), supplemented with 10% heat-inactivated fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Immunology 2022Quote: ... and Toledo virus was conjugated with DMSO-dissolved AF647–N-hydroxysuccinimide ester (Invitrogen) with constant agitation at room temperature for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... the master mix used was TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Manufacturer’s protocol was followed to add the appropriate volumes of master mix ...
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bioRxiv - Microbiology 2022Quote: Purified virus particles were incubated with different concentrations of BS3 cosslinkers (ThermoFisher Scientific) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml, Gibco) 24h later ...
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bioRxiv - Neuroscience 2022Quote: ... PBMCs were expanded and reprogrammed using Sendai virus vector (CytoTune-iPS 2.0, Invitrogen) using a feeder-free system (Sommer et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (ThermoFisher Scientific). Real-time PCR was performed using TaqMan assays (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... MCC virus-positive MKL-1 cells were cultured in 1x RPMI (ThermoFisher Scientific) supplemented similarly as stated above ...
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bioRxiv - Immunology 2023Quote: ... RNA from virus stocks was purified using TRIzol Reagent (Thermo Fisher Scientific, #15596026). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The virus-containing media were filtered through a 0.45 µm filter (ThermoFisher Scientific) and mixed with fresh media at a 1:1 ratio before adding to pre-seeded target cells ...
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bioRxiv - Immunology 2024Quote: ... the media was changed to virus production medium containing Opti-MEM I (Gibco) + 5 % FBS + 1:100 Glutamax + 1:100 MEM-NEAA (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Nuclease treatment of virus stocks was performed at room temperature by DNase (Ambion), RNase (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...
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bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...
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bioRxiv - Biochemistry 2024Quote: ... cells were given virus production media (DMEM supplemented with 20mM HEPES (Gibco; 15630080) and 30% FBS) ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was prepared using Moloney Murine Leukemia Virus reverse transcriptase (Thermo Fisher Scientific) with Random Hexamers (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... these plasmids were transiently transfected into HEK293T cells with packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 3000 transfection reagent (Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... we introduced a stop codon in the pLRB302 plasmid at residue 640 of the envelope ORF (C640Stop) located at the N’ terminal of the cytosolic tail using the Phusion Site-Directed Mutagenesis Kit (Thermo Fisher Scientific) and the following primers ...
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bioRxiv - Immunology 2020Quote: ... PSG3 5 plasmid was co-transfected into 293T cells along with various HIV envelope plasmids 6 using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific, #11668019) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...
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bioRxiv - Cell Biology 2019Quote: ... The RNAi libraries used were a custom library targeting gene products predicted to localize to the nuclear envelope (346 genes, ThermoFisher, lot# AMO20JUZ), and an off the shelf library targeting proteins involved in epigenetic and chromatin regulation (521 genes ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 million HEK 293FT cells were seeded and transfected after 24 hours with the LentiCRISPRV2-IL11g1 or LentiCRISPRV2-IL11g2 construct together with the pLp Δ8,9 packaging plasmid and VSVG envelope plasmid using lipofectamine™ 2000 transfection reagent (Life Technologies, 11668019) and Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Synthetic Biology 2023Quote: ... and envelope plasmids into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 according to the manufacturer’s instructions (ThermoFisher Scientific, MA, USA). Cell supernatant containing lentivirus was collected at 24 and 48 hours ...
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bioRxiv - Synthetic Biology 2021Quote: ... Lysate from a strain containing empty pET51b+ vector (Invitrogen, Waltham MA) was analyzed as negative control for background endogenous transacylation by E ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... and purified from the BL21 (DE3) Arabinose-Inducible bacterial strain (Invitrogen) with an adaptation from the same purification protocol (McDuff et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... cerevisiae strain background (S288C, MATa HIS3Δ1 LEU2Δ0 MET15Δ0 URA3Δ0, Thermo Fisher) was used for all experiments in this study ...
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bioRxiv - Biochemistry 2020Quote: ... Ded1-TAP and eIF4A-TAP strains were obtained from Thermo Scientific Open Biosystems (Waltham ...
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bioRxiv - Biochemistry 2020Quote: ... were transformed into Escherichia coli production strain Rosetta (DE3) cells (Invitrogen). Production of the maltogenic α-amylase LaGH13_20 and the oligosaccharide 1,4-α-glucosyltransferase LaGH13_31B was carried out using a 5 L bioreactor (Biostat B ...
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bioRxiv - Microbiology 2021Quote: Mouse liver microsomes (CD-1 male strain) were purchased from GIBCO. Hamster (Syrian female strain ...
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bioRxiv - Microbiology 2021Quote: ... falciparum 3D7and PfKelch13R539T strain was isolated using TRIZOL (Life Technologies, USA). Equal amount of total RNA was used for cDNA synthesis using the cDNA synthesis Kit (Thermo fisher ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae strain D39 diluted in phosphate-buffered saline (PBS; Thermo Scientific) into the lateral tail vein [15 ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain BL21-star (DE3) (Thermo Fisher Scientific, Waltham, MA, USA), and the transformed cells were cultured in Luria–Bertani medium at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... while knockout strains were taken from the Yeast Knockout Collection (Invitrogen).
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bioRxiv - Microbiology 2021Quote: FAS was purified from PichiaPink™ yeast strain one (Invitrogen, USA), which was grown according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli BL21 (DE3) strain was purchased from Invitrogen (California, USA). Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt ...
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bioRxiv - Microbiology 2019Quote: Strains were grown in LB medium (Fisher Scientific, cat # BP1426-2) at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... or TgGPNΔSRD-expressing strains were harvested and lysed in TriReagent (Invitrogen). RNA was extracted according to the manufacturer’s instructions and further cleaned with PureLink Mini RNA and the concentration was calculated on a Nanodrop (Thermo) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The “BL21” strain is the BL21 Star™ (DE3) (Fisher Scientific) strain ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 Star™ (DE3)pLysS strain (#C602003, Thermo Fisher Scientific) was transformed with pDest-His6-MBP-PDE6D and grown at 37 °C in LB medium supplemented with ampicillin at 1:1,000 dilution from 100 mg/ mL stock ...
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bioRxiv - Biochemistry 2023Quote: ... coli strain was BL21(DE3) from Invitrogen (One ShotTM BL21(DE3), Cat ...
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bioRxiv - Microbiology 2023Quote: ... or 6 was then used to transform into stbl3 strain (Invitrogen) for further plasmid amplification.
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bioRxiv - Microbiology 2023Quote: ... Strains were cultured in brain heart infusion (BHI, Fisher Scientific, NH) at 37 °C under different oxygen conditions as specified in the main text ...
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bioRxiv - Microbiology 2024Quote: ... Escherichia coli strains were grown in Luria-Bertani (LB; ThermoFisher Scientific) medium at 37 °C in a MaxQ 6000 incubator (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... gondii strains were cultured in Lab-Tek chamber slides (177437, Nunc). Infected cells incubated or not with the chelator were then chemically fixed for 2h at room temperature using the fixation solution (2.5% glutaraldehyde in 0.1 M Cacodylate buffer ...
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bioRxiv - Biochemistry 2019Quote: ... lentivector plasmids were transfected into HEK293T cells together with packaging and envelope plasmids (psPAX2 and pMD2.G) using Lipofectamine 3000 (Thermo Fisher Scientific, L3000008). Three days after transfection ...
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bioRxiv - Immunology 2022Quote: ... 2.7 μg envelope plasmid pMD2G and 8 μg target plasmid encoding shRNA or lentiCRISPRv2 encoding sgRNA using Lipofectamine® 2000 (Thermo Fisher Scientific). Lentivirus supernatants were collected at 36 h ...
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bioRxiv - Immunology 2021Quote: ... qPCR was performed using TaqMan Fast virus 1 Step PCR Master Mix (Life Technologies), standard curve was drawn with 2019_nCOV_N Positive control (IDT) ...
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bioRxiv - Immunology 2022Quote: ... amplified using the TaqMan Fast Virus 1-Step Master Mix qRT-PCR kit (Invitrogen) on a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... and the virus was produced in HEK293A cells as described by the manufacturer (Invitrogen). Immature brown adipocytes were treated with the viruses on day 2.