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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Sheep Anti CMV Pentamer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... or FLAG:ACAD11 expressed under a CMV promoter in pcDNA3.1 constructs using Lipofectamine 3000 (Thermo Scientific) in OptiMEM (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 ng of PGL4 (hRluc-CMV) plasmid using lipofectamine-3000 as transfection reagent (Invitrogen). For RU486 treatment ...
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bioRxiv - Microbiology 2019Quote: ... The isolates were grown on sheep blood agar plates (Thermo Fisher Scientific, Remel, Lenexa, KS) at 42°C under microaerobic conditions (85% N2 ...
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bioRxiv - Cancer Biology 2021Quote: ... PA5-51057 and sheep IgG isotype control (Cat. No. 31243) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Immunology 2022Quote: ... The bacteria were grown on blood agar plates (TSA with 5% sheep blood, Thermo Fisher). Inoculated plates were placed into a hypoxia chamber (Billups- Rothenberg) ...
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bioRxiv - Microbiology 2021Quote: ... or the respective dilutions at the indicated times onto sheep blood agar plates (ThermoFisher, Spain). Plates were incubated for 24 h at 37 °C ...
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bioRxiv - Physiology 2022Quote: ... 2 µg/ml Heparin) and secondary antibodies against sheep (1:500, Invitrogen, A21436 or A21448) and/or rabbit (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... immunofluorescently stained for BUNV-NP (α-sheep Alexa-fluor-594 secondary antibodies; Thermo Scientific A11016) and images taken using an IncuCyte ZOOM live imaging system as previously described (10,45).
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bioRxiv - Cancer Biology 2021Quote: ... The iRFP-ENTR4 was then recombinated into the pLenti-CMV-Puro-Dest by a LR (Invitrogen) gateway reaction as previously described 20 ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... Successful incorporation of pShuttle-CMV construct into AdEasier-1 cells confirmed via digestion with PacI (ThermoFisher). 5000 ng plasmid was then digested at 37℃ overnight ...
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bioRxiv - Biophysics 2020Quote: ... pLenti CMV Hygro 3xnls-mScarlet-I was generated by Gateway Gene Cloning (Invitrogen, Thermo Fisher Scientific). First ...
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bioRxiv - Biophysics 2020Quote: ... pLenti CMV Hygro 3xnls-mScarlet-I was generated by Gateway Gene Cloning (Invitrogen, Thermo Fisher Scientific). First ...
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bioRxiv - Cell Biology 2020Quote: ... and pLV-CMV-delta 8.2 (Massey, A.C 2006) using the Lipofectamine LTX transfection reagent (Life Technologies). Virus-containing supernatants were collected at 24 and 48 h post transfection ...
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bioRxiv - Microbiology 2019Quote: ... We generated the corresponding vector by cloning eGFP into pcDNA3.3-CMV (Thermo Fisher Scientific, Waltham, MA) modified by an in-frame C-terminal V5 peptide tag.
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bioRxiv - Physiology 2021Quote: ... musculus Crebrf (Uniprot Q8CDG5-1) were constructed using the pAd/CMV/V5-DEST vector (Thermo Fisher). No epitope tag was added to the CREBRF polypeptide ...
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bioRxiv - Cancer Biology 2022Quote: ... CMV-Rev (6.25 μg) and VSV-G (9 μg) using the calcium-phosphate transfection method (Invitrogen). The transfection mixture was added to the packaging cells along with 100 mM chloroquine (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... A CMV expression plasmid encoding Sumo-HRV-CD19-Spytag-Histag was transfected into Expi293F Cells (ThermoFisher) using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... thetaiotaomicron were cultured on trypticase soy-agar plates with 5% v/v sheep blood (ThermoFisher #B21239X) at 37°C while contained within an anaerobic chamber (BD GasPak #260683) ...
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bioRxiv - Microbiology 2020Quote: Hemolytic activity was measured using Mueller-Hinton agar plates containing 5% defribinated sheep blood (Thermo Fisher) +/-L-161,982 at a final concentration of 50 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Presumptive macrococci colonies were streaked onto blood agar supplemented with 5% sheep blood (Oxoid, ThermoFisher, Johannesburg), incubated for 24 hours at 37°C ...
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bioRxiv - Genomics 2020Quote: Expression cassettes consisting of the doxycycline-inducible cytomegalovirus (CMV) promoter (derived from the Invitrogen T-REx system), the intron-containing EF1α 5 UTR ...
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bioRxiv - Neuroscience 2020Quote: ... Final constructs were subcloned into mammalian expression plasmids pIR-CMV (NaV1.1, NaV1.2) (18) or pcDNA4/TO (NaV1.6; ThermoFisher). Each construct included an internal ribosome entry sequence (IRES2 ...
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bioRxiv - Cell Biology 2021Quote: ... Successful incorporation of pShuttle-CMV construct into AdEasier-1 cells were confirmed via digestion with PacI (ThermoFisher). 5000 ng plasmid was then digested at 37°C overnight ...
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bioRxiv - Immunology 2022Quote: Lentivirus construction encompassing BAFF was generated using the AdMax (Microbix) and pSilencer™ adeno 1.0-CMV (Ambion) systems ...
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bioRxiv - Cell Biology 2023Quote: ... the promoter reporter vector and the pRL-CMV Renilla luciferase plasmid using Lipofectamine 2000 reagent (Invitrogen, USA). After transfection for 24 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting clones were transferred into pLenti CMV GFP Blast 101 or pcDNA3.1/nV5-DEST (ThermoFisher Scientific, 12290010) by LR recombination (ThermoFischer Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... or pLenti-CMV-Dendra2×3-P2A-H2B-miRFP after a 5 minute incubation in OptiMEM (Gibco, #31985070) with 40 µg linear PEI (polsciences ...
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bioRxiv - Immunology 2024Quote: pCMV-mCD8 carries the mouse CD8 α-chain driven by the CMV immediate early promotor/enhancer (Invitrogen). The Adenoviral expression vectors ...
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bioRxiv - Molecular Biology 2021Quote: ... at 37°C without aeration or in THY agar medium supplemented with 5% sheep blood (Thermo Scientific) at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Microbiology 2021Quote: Hemolytic index was measured using 1.5% BHI agar plates supplemented with 5% defibrinated sheep blood (Thermo Scientific). After overnight incubation at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... pneumoniae (one colony per species per plate) were further sub-cultured on Sheep Blood Agar plates (ThermoFisher) and species identity confirmed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS ...
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bioRxiv - Microbiology 2022Quote: ... Transformants were selected by plating inside Columbia agar supplemented with 3% defibrinated sheep blood (CBA, Thermo Scientific) containing the appropriate antibiotic and incubated at 37°C in 5% CO2 overnight ...
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bioRxiv - Microbiology 2020Quote: ... Transformants were selected by plating inside Columbia agar supplemented with 3% of defibrinated sheep blood (Thermo Scientific), followed by CRISPR-mediated counter selection ...
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bioRxiv - Neuroscience 2022Quote: ... Actual inocula were determined by plating serial dilutions on sheep blood agar plates (Thermo Fisher, Waltham, MA). Age-and sex-matched 20–24-week-old ...
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bioRxiv - Cell Biology 2020Quote: ... 293T cells were transfected with pLenti-CMV-eGFP plasmid together with a lentviral packaging mix (Life Technologies, USA) to produce GFP lentivirus ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A fragment containing CMV-2TO-MCS-bGH-poly(A) was PCR amplified from pcDNA4/TO (Thermo Fisher, V102020) using primers CMV_bGH_F and _R ...
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Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... The cytomegalovirus (CMV) promoter was amplified by PCR from the pcDNA 3.1D/V5-His-TOPO plasmid (Life Technologies) with forward primer 5’-AGTAATCAATTACGGGGTCATTAGTTCAT-3’ and reverse primer 5’ CATAGGAGATGCCTAAGCCGGTGGAGCTCTGCTTATATAGACCTC-3’ ...
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bioRxiv - Immunology 2023Quote: ... pRL-CMV-Renilla (5 ng) and pReceiver-M12-SH2B3 (100 ng) plasmids using Lipofectamine 2000 (Invitrogen; cat # 11668019) resuspended in Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... HT29 cells were seeded in a 6-well plate upto 50-60% confluency and transfected with 2.5μg of cloned PGL4.12 and 50ng of PGL4 (hRluc-CMV) plasmid using lipofectamine-3000 as transfection reagent (Invitrogen). The transfected cells with cloned pGL4.12 and pGL4 (hRluc-CMV ...
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bioRxiv - Immunology 2024Quote: ... KP cells were transiently transfected with pZac2.1-U6sgRNA-CMV-ZsGreen and (pSpCas9(BB)(PX458) using Lipofectamine 3000 (Invitrogen), following the manufactureŕs instructions ...
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bioRxiv - Microbiology 2024Quote: ... This CMV-DENV genome-HDVr-SV40pA assembled product was transfected into BHK-21 cells using Lipofectamine 2000 (Invitrogen). Four to six days later ...
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bioRxiv - Immunology 2022Quote: ... Lysate was cultured overnight at 37°C on agar medium containing sheep blood (Fisher Scientific, cat no. PB5012A).
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bioRxiv - Neuroscience 2020Quote: ... rabbit or sheep antigens were raised in donkey and conjugated to Alexa 488 or Alexa 594 (Molecular Probes, ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... pneumoniae transformants were selected by plating inside Columbia agar supplemented with 3% of defibrinated sheep blood (Thermo Scientific), followed by antibiotic selection ...
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bioRxiv - Microbiology 2023Quote: ... cells from a cryostock were streaked onto Tryptic Soy Agar (TSA) with 5% sheep blood (Thermo Scientific, R01198) and incubated for two days in an anaerobic chamber (with 2-3% hydrogen ...
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bioRxiv - Microbiology 2024Quote: ... tryptic soy agar with sheep’s blood (pre-made plates: Thermo Scientific; tryptic soy agar: BD Difco, Fisher Scientific; defibrinated sheep’s blood ...
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bioRxiv - Cell Biology 2020Quote: ... Tor1A and Tor1B coding regions were amplified from HeLa cDNA and cloned into the vector pcDNA5.1-GFP FRT/TO (cytomegalovirus [CMV] promoter; Invitrogen) for generation of stable ...
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bioRxiv - Microbiology 2020Quote: ... MDCK-CMAH cells were prepared by transfection of the human CMAH gene in a plasmid under the control of the CMV promoter (pcDNA3.1, Invitrogen). Clones with Neu5Gc expression were determined by HPLC analysis as previously described (63) ...
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bioRxiv - Microbiology 2020Quote: ... MDCK-SiaT1 cells were prepared by transfection of the ST6Gal1 gene in a plasmid under the control of the CMV promoter (pcDNA3.1, Invitrogen). Cell clones with increased levels of α2,6-linked Sia were identified by staining with the Sambucus nigra (SNA ...
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bioRxiv - Microbiology 2019Quote: ... Variants of those MDCK cells with increased levels of α2,6-linked Sia cells were prepared in the laboratory by transfection with the ST6Gal1 gene in a plasmid under the control of the CMV promoter (pcDNA3.1, Invitrogen). Cell clones with increased levels of α2,6-linked Sia were identified by staining with the Sambucus nigra (SNA ...