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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Sheep Anti CMV Pentamer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... and a non-targeting pre-miRNA under bicistronic control of the CMV promoter in the pcDNA6.2GW/EmGFP-miR plasmid (Invitrogen). The UBR5 RNAi plasmid encodes EmGFP and an artificial pre-miRNA targeting mouse UBR5 under bicistronic control of the CMV promoter ...
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bioRxiv - Physiology 2020Quote: ... and a non-targeting pre-miRNA under bicistronic control of the CMV promoter in the pcDNA6.2GW/EmGFP-miR plasmid (Invitrogen). The UBR5 RNAi plasmid also encodes EmGFP plus an artificial pre-miRNA targeting mouse UBR5 under bicistronic control of the CMV promoter ...
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bioRxiv - Molecular Biology 2020Quote: ... pAAV-CMV-eGFP3 was co-transfected into PC3 cells with TRIBE construct using Lipofectamine™ 2000 (Thermo Fisher Scientific). One day or two days later ...
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bioRxiv - Developmental Biology 2019Quote: ... entry vectors were recombined with the adenovirus type 5 destination vector pAd5-CMV-V5-DEST using LR Clonase (Invitrogen). pAd5 vectors carrying candidate gene vectors were further sequenced to confirm gene identity ...
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bioRxiv - Evolutionary Biology 2019Quote: ... or HDHD-NDA-RA-pGL4.27 along with 500 ng pRL-CMV (Renilla reporter plasmid) via electroporation (Neon Transfection System, Invitrogen). Firefly and Renilla luciferase activity were analyzed using the Dual-Glo Luciferase Assay System (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... Transfections of transfer vector (pLentiCRISPRv2 SCRA, pLentiCRISPRv2 LAMA5) and packaging plasmids (CMV-VSVg and Delta8.9) were performed using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Successful incorporation of pShuttle-CMV construct into AdEasier-1 cells confirmed via digestion with PacI (Thermo Fisher Scientific, IVGN0184). 5000 ng plasmid was then digested at 37°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... For overexpression of miR-7704 the MDA-MB-231 cells were grown in six-well plates and transfected for 48h with pPRIME-miR-7704-CMV-GFP-FF3 plasmid (5.5 µg/well) using Lipofectamine-2000 reagent (ThermoFisher). As control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pAAV-CMV-tKiMBI-T2A-caMEK-bGH_pA plasmids were purified by PureLink Expi Endotoxin-free Maxi plasmid Purification kit (Invitrogen), and then were sent to Stanford Gene Vector and Virus Core to produce and titer AAV.DJ vectors.
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bioRxiv - Developmental Biology 2020Quote: ... diluted (1:10,000) in pre-incubation (PI) buffer (PBS, 0.1% Tween 20, 2% sheep serum, 2mg/ml BSA (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The cell suspension was then inoculated on LBN agar plates containing 5 % (v/v) defibrinated sheep blood (ThermoFisher Scientific) for haemolysin assay and 1 % (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit or sheep antigens were raised in donkey and conjugated to Alexa 488 or Alexa 594 (Molecular Probes, ThermoFisher, RRIDs ...
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bioRxiv - Microbiology 2021Quote: ... and 2 μL of each culture were spotted onto a TSA plate containing 5% sheep’s blood (Thermo Fisher, R060312). The plates were incubated at 30°C for 72 h and imaged above a white light ...
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bioRxiv - Microbiology 2020Quote: ... pylori was cultured on Trypticase soy agar with 5% (v/v) sheep blood (Thermo Fisher Scientific, Waltham, MA, USA) for 2 days at 37°C in microaerobic conditions ...
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bioRxiv - Microbiology 2021Quote: ... All strains were grown on Columbia agar supplemented with 7% (v/v) sheep blood (Oxoid™, Thermo Fisher Scientific) overnight at 37°C under aerobic conditions ...
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bioRxiv - Microbiology 2023Quote: ... a loopful of bacteria from the lysis spots were streaked on sheep blood agar (SBA, ThermoFisher, Australia, #Cat: R01202) and incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Wild type and catalytic mutant H672N RNase L plasmids cDNAs (the backbone is pcDNA5-FRT-TO with CMV promoter, Invitrogen) used in this study were a generous gift from Dr ...
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bioRxiv - Molecular Biology 2020Quote: ... A fragment encoding the CMV promoter and mCherry-T2A-Cre-WPRE was excised by NdeI and SacII (Thermo Fisher Scientific) and transferred to the pLenti6/V5-DEST™ Gateway™ Vector (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... mCherry-(backbone pLV-CMV-bc) and FLAG-His-tagged(backbone Pcdna3) p16INK4A constructs were created using gateway technology (Life Technologies). For cell cycle profiling ...
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bioRxiv - Neuroscience 2020Quote: ... cells were seeded at a density of 3.105 cells/well 24h before transfection (2 μg of pLVX-CMV DNA/well) with lipofectamine 2000™ according to the manufacturer’s instruction (Invitrogen). The SH-SY5Y were treated with 300 nM of trichostatin A (TSA ...
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bioRxiv - Synthetic Biology 2019Quote: ... 100 ng/well of each amplicon or 400 ng/well of each plasmid was used) and 10 ng/well pRL-CMV control vector were cotransfected into cells with Lipofectamine 2000 (Invitrogen) reagent following a standard protocol ...
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bioRxiv - Microbiology 2020Quote: The Zaire EBOV GP gene was expressed using a pcDNA vector under control of the CMV promoter (Invitrogen, Carlsbad, CA) (54 ...
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bioRxiv - Molecular Biology 2021Quote: Adenoviruses expressing the Flag-TSC22D4 cDNA under the control of the CMV promoter was cloned using the BLOCK-iT Adenoviral expression system (Invitrogen). Viruses were purified by the cesium chloride method and dialysed against phosphate-buffered-saline buffer containing 10% glycerol before animal injection ...
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bioRxiv - Cancer Biology 2021Quote: ... and transfer plasmid (N174-CMV-GFP-miR-E-IRES-puro-shRNA) into HEK293T cells with Lipofectamine 2000 reagent (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant AAV6.2 vectors were produced by calcium phosphate transfection of human embryonic kidney (HEK)-293 cells using the pFB_CMV-hDTR or the pFB_stuffer plasmid in combination with a pAAV-Cap6.2 (56) and the pHelper plasmid (Thermo Fisher Scientific), followed by purification and titer determination as described previously (57).
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bioRxiv - Cell Biology 2022Quote: ... CMV-mPiezo1 was previously generated in-house by subcloning mouse Piezo1 into the pcDNA5/FRT expression vector (Fisher Scientific, V601020) – this plasmid was used for all calcium imaging of Piezo1 activity ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with the packaging plasmids psPAX2 and pCMV-VSV-G along with the transgene in pLenti-CMV-GFP-Puro using Lipofectamine 2000 (Invitrogen). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454 ...
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bioRxiv - Physiology 2023Quote: An expression clone for the adenoviral vector was then generated by performing a LR recombination reaction between the entry clone and pAd/CMV/V5-DEST (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were co-cultured with HLA-B/CMV-restricted CD8+ T-cells for five hours (E:T ratio: 3:1) in RPMI 1640 medium (Life Technologies)(supplemented with 10 % (v/v ...
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bioRxiv - Plant Biology 2023Quote: ... Verified entry clones were used to transfer the CMV 2b sequences into pSITE (Chakrabarty et al., 2007) destination plasmids following incubation with LR clonase (Invitrogen) for 1 hour at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... pME-Dmist was recombined with 5’ (p5E-CMV/SP6) and 3’ (p3E-GFPpA) entry clones and destination vector (pDestTol2pA2) using Gateway Technology (Invitrogen LR Clonase II Plus enzyme Cat No ...
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bioRxiv - Cell Biology 2023Quote: Generation of replication-defective recombinant adenovirus expressing N-SREBPs under CMV promoter was performed using ViraPower Adenoviral Expression System (#K4930-00, Invitrogen) as described earlier (54) ...
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bioRxiv - Neuroscience 2021Quote: ... Then slides were washed 3×5’ in TBS and incubated in donkey-α-sheep-488 (1:500, Life Technologies, A11015) in TBS for 90’ ...
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bioRxiv - Cancer Biology 2022Quote: ... or normal IgG (1μg) was incubated with 50 μl Dynabeads M-280 sheep antimouse IgG magnetic beads (Thermo Fisher, 11202D) in 200ul wash buffer (50 mM pH 7.5 Tris-HCl ...
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bioRxiv - Pathology 2019Quote: ... Aliquot concentration was determined by enumerating colony growth on agar plates with 5% sheep blood (Remel, Thermo Fisher, Lenexa, KS) at the time of freezing ...
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bioRxiv - Microbiology 2019Quote: ... Any typical colony was streaked to a trypticase soy agar plate supplemented with 5% defibrinated sheep’s blood (Thermo Scientific-Remel) to confirm isolate purity ...
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bioRxiv - Microbiology 2022Quote: ... Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922) were passaged twice on tryptic soy agar with 5% sheep’s blood (Thermo Scientific) at 37° C in 5% CO2 for 18-24 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... was grown overnight on blood agar plates (with 5% sheep blood) or in brain heart infusion broth (SA, ThermoFisher Scientific) until OD600=0.4 ...
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bioRxiv - Microbiology 2023Quote: ... serial dilutions of the cryopreserved homogenates were plated on eight different media: CBA (Columbia Blood Agar supplemented with 5 % defibrinated sheep blood (Thermofisher)) ...
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bioRxiv - Immunology 2023Quote: ... The depletion of endogenous microbiota was monitored by conducting quantitative fecal cultures on Sheep Blood agar plates (Thermo Scientific, #R01200). This allowed the assessing of the reduction in microbial colony-forming units (CFUs ...
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bioRxiv - Microbiology 2023Quote: ... The culturing for all the samples was done onto tryptic soy agar with 5% sheep’s blood (Thermo Fisher Scientific, USA). The plates were incubated at +35±2°C in 5% CO2 and read at 24-hour intervals for four days ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial cultures were plated on THM-blood-agar consisting of 5% of sheep blood (Remel Lenexa, KS) in THM with 1.5% agar (Fisher Scientific). The bacteria were routinely cultured in an anaerobic chamber in an atmosphere consisting of 80% N2 ...
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bioRxiv - Microbiology 2024Quote: Additional homogenised tumour tissues from four patients (PT09, PT14, PT15, PT17) were also cultured on sheep blood agar plates (Thermofisher) in an anaerobic hood at 37°C condition ...
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bioRxiv - Biophysics 2019Quote: ... and subsequently cloned into CMV-mCherry-Gateway vector or Vivid Colors™ pcDNA™6.2/N-EmGFP-DEST vector (ThermoFisher Scientific) to obtain CMV-mCherry-DBNL or CMV-EmGFP-DBNL ...
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bioRxiv - Biophysics 2019Quote: ... N-terminal-tagged MBP-TRPA1 under the control of a CMV promoter was expressed in HEK293F cells using the BacMam system (Thermo Fisher). Protein expression was induced at cell density 1.5-2.5 × 106 cells ml- with media supplemented with 5 mM sodium butyrate and 5 µM ruthenium red ...
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bioRxiv - Microbiology 2020Quote: ORFs were cloned into the pENTR1A construct and transferred into the pLENTI CMV puro or blast vectors using a Gateway enzyme mix (Invitrogen #56484). Lentivirus was produced by co-transfection of the pLenti CMV plasmids with pMD2.G and psPAX2 plasmids ...
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bioRxiv - Neuroscience 2019Quote: ... 1.5 ug of CMV-Egr3 vector or CMV vector was co-transfected with 1 ug of each promoter reporter vector per well using Lipofectamine 3000 reagents (ThermoFisher Scientific). Transfection was performed with 3.75 μl of Lipofectamine 3000 reagent ...
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bioRxiv - Microbiology 2020Quote: ... 3.6 µg pSp-Cas9(BB)-2A-GFP-sgRNA and 400 ng pSc-TIA- CMV-BSR-TIA were mixed with polyethylenimine and OptiMEM (#51985026, ThermoFisher Scientific) for transfection of cells ...
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bioRxiv - Systems Biology 2020Quote: 5637 cells were placed in 24 wells plate and transfected with the lentiviral vectors pTSB-CMV-puro and SiRNA against ACSS1 reaching 70%-80% confluence using Lipofectamine 2000 (Thermo Scientific) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... pCMV-EML4-ALK(K589M)-P2A-H2B-iRFP and CMV-3XFLAG-mCh-EML4-ALK were transiently transfected into wt Beas2B or Beas2B mNG:Grb2 cells using Lipofectamine™ 3000 (Invitrogen, L3000001). Briefly ...