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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 20 % FBS and 200 ng/mL Human M-CSF Recombinant Protein (ThermoFisher). Six days after the initial isolation ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Bioengineering 2021Quote: ... + 2% human serum albumin (ThermoFisher) + 5 μM ROCKi (Tocris) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell death was measured via treatment with Sytox Blue (Thermo Fisher Scientific, Waltham, MA). Sytox Blue was diluted in PBS and added to the media of all wells at a final concentration of 0.5 μM and incubated in the dark at 37°C for 15 minutes ...
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bioRxiv - Genomics 2020Quote: ... Cell death was quantified by addition of propidium iodide (PI) (0.1 mg/mL, ThermoFisher) to the culture medium for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Cell death was evaluated using flow cytometry (LIVE/DEAD™ Violet Viability dye (ThermoFisher)) and infection was evaluated by either flow cytometry (intracellular p24 staining ...
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bioRxiv - Plant Biology 2023Quote: ... cell death was measured by labelling with the fluorescent dye SYTOX Green (ThermoFisher Scientific) as described previously (Possmayer et al. ...
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bioRxiv - Immunology 2023Quote: Cell death assays were performed using the Attune Nxt Acoustic Focusing Cytometer (Life Technologies). Cells were seeded in 6-well plates at a density of 1×106 cells per well ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability/death was determined by Click-iT Plus Edu and PI staining (Thermofisher).
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and seeded onto new 6-well plates at 2,500 cells/cm2 with 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700). After allowing cells to adhere overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (ThermoFisher Scientific, Waltham, MA, USA) (S12+ media ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected 24 hr after treatment with the cell death inducers with TrypLE Express (Life Technologies) and resuspended in PBS containing 0.5% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: The DNA coding sequences of tagged recombinant GITRL and OX40L were produced by GeneArt Gene Synthesis service (ThermoFisher Scientific). The sequences of GITRL and OX40L constructs contain ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (MA1-21315, Invitrogen, 1:1,000). Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system ...
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bioRxiv - Microbiology 2020Quote: ... We then resuspended the cell pellet in 9.5mL PBS/2%HI-FBS for counting using the Countess Cell Counting system (Thermo Fisher Scientific). We aliquoted 0.5 mL for Seq-Well ...
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bioRxiv - Microbiology 2023Quote: ... 2 µL of Novex Hi-Density TBE 5X Sample Buffer (Invitrogen) was mixed with 8 µL of the completed EMSA reaction and loaded on an 8% acrylamide gel ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by death confirmation) and embedded in 1% low melting point (LMP) agarose (Invitrogen) with the tumour or regressed tissue oriented to the bottom of 6-well glass bottom plate (Cellvis) ...
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bioRxiv - Biochemistry 2022Quote: ... His6- tagged SPOC domains were purified by affinity chromatography using HisTrap HP column (Cytiva) or His-Pur Ni-NTA resin (Thermo Scientific) equilibrated in 25 mM Tris-Cl pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... the N-terminal His-tagged protein was purified in a single step protocol using HisPur Ni-NTA resin (Thermo Fisher Scientific), equilibrated at RT ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: Human THP-1 cells were cultured in RPMI-1640 medium containing 2 mM L-glutamine (Gibco; Grand Island, NY), 10% FBS ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μL of serially diluted ligands were aliquoted into MicroAmp Optical 96-Well Reaction Plates (ThermoFisher Scientific). Plates were then prepared using 18 μL of a 1.11 × master mix according to the Protein Thermal Shift™ Dye Kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: To compare the presence of the nucleolar rim of mNG-tagged proteins in live and fixed cells we fixed mNG-tagged cells in 4% formaldehyde (Thermo Scientific, 28908).
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bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
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bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
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bioRxiv - Immunology 2022Quote: ... protein was evaluated in lysate cells and the type of cell death was evaluated in detached cells after treatment with trypsin (Gibco). For treatment experiment ...
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bioRxiv - Immunology 2021Quote: ... and recombinant epidermal growth factor (EGF, 2 ng/ml, Gibco).
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bioRxiv - Synthetic Biology 2021Quote: ... and re-suspended at a density of 104 cells/mL in pre-warmed complete minimum essential media (MEM) that contains 5 mg/mL human recombinant insulin (Gibco), 1X MEM non-essential amino acids (NEAA ...
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bioRxiv - Genomics 2020Quote: ... Both cell lines were maintained in culture vessels coated with a matrix of Vitronectin human recombinant protein (ThermoFisher Scientific, A14700) and batch fed daily with mTeSR (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2022Quote: The human embryonic stem cell line H9 (WiCell, authenticated at source) was grown on recombinant human vitronectin (Thermo) coated cell culture dishes in StemFlex Medium (Gibco). Subculturing was performed by incubating plates with 0.5 mM EDTA for 5 minutes and then resuspended in culture medium to be transferred to new coated plates.
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bioRxiv - Developmental Biology 2023Quote: MAN13 hESCs were expanded in 6-well cell culture plates previously coated with 5µg/ml Human Recombinant Vitronectin (Gibco, #A27940) in Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific), and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 10 ng/ml.
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 10 ng/ml.
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were expanded on human recombinant vitronectin in StemFlexTM medium (Thermo Fisher Scientific). iBEC differentiation was performed as previously described by us [18] ...
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bioRxiv - Biochemistry 2019Quote: ... LRRK2 (G2019S) Recombinant Human Protein (residues 970-2527 #10499963) was purchased from Invitrogen and all additional kinases used in the in vitro screen were from MRC PPU Reagents and Services – including TAK1 (1-303) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human EGF and PDGF-BB were purchased from Gibco (Catalog No. PHG0311) and Peprotech (Catalog No ...
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bioRxiv - Microbiology 2022Quote: ... human recombinant epidermal growth factor (0.2 ng/mL, all from Gibco, Schwerte, Germany), isoproterenol (1 nM ...
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bioRxiv - Bioengineering 2022Quote: ... or 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700) for serum-free cells ...
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bioRxiv - Neuroscience 2022Quote: ... 10 µg/mL human recombinant insulin (ThermoFisher Scientific #12585014; stock = 4 mg/mL), 1:100 Penicillin-Streptomycin (ThermoFisher Scientific #15140122) ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Cell Biology 2023Quote: ... all iPSCs were cultured in Vitronectin Recombinant Human Protein (VTN-N) (ThermoFisher, A14700) pre-coated 6-well plates with 2 mL TeSR-E8 medium (Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... and cell death was analyzed using TUNEL staining according to the manufacturer’s protocol (Invitrogen, C10245). Images were acquired using Keyence BZ-X710/810 microscope.
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bioRxiv - Immunology 2020Quote: ... Cell death was assessed by 7-aminoactinomycin D (7-AAD, Affymetrix eBioscience, San Diego, CA) staining and flow-cytometry (FACSCantoII™ ...
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bioRxiv - Microbiology 2022Quote: Cell death in planktonic cultures was detected using the BacLight Bacterial Viability kit (L7007, Invitrogen) and quantified by fluorimetry ...
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bioRxiv - Immunology 2021Quote: ... at the concentration 1×106 cells per ml for TNF-α assessment in myeloid cells or Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) for IFN-γ assessment in T-cells in the presence of protein transport inhibitor (BD GolgiStop™ ...
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bioRxiv - Cell Biology 2021Quote: ... reaction mixes were prepared using 2.5 μg recombinant His-ACBD5 with or without the addition of 0.3 mM ice-cold ATP (Thermo Fisher) and 0.1 ug GST-GSK3β (Abcam) ...