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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system ...
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bioRxiv - Genomics 2024Quote: ... 20 ng/mL human recombinant EGF (Thermo Fisher PHG0311), and 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2024Quote: ... growth factor 100ug/ml FGF10 (recombinant human) ( Thermo Fisher) and 500nM A-83-01 (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... and digest protocol was programmed using BindIt software v4.1 (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant human Epithelial Growth Factor 5 µg.L-1 (Ref AF-100-15; PEPROTECH, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DMEM 2% Hi-FBS 0.5% Ultrapure agarose (Invitrogen) was used to overlay the wells ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% HI-FBS and 2mM EDTA (Invitrogen, 15575038).
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bioRxiv - Microbiology 2021Quote: The production of His-tagged soluble ACE2 was performed in FreeStyle™ 293-F cells and purified using HisPur™ Ni-NTA Resin (Thermo Scientific) as described previously [31].
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bioRxiv - Synthetic Biology 2020Quote: ... and the His-tagged proteins on the cell surface were then visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, USA) using FUSION Solo S documentation system (Vilber ...
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bioRxiv - Neuroscience 2022Quote: Cell death was detected using Propidium iodide (PI, Thermo Fisher Scientific) or SYTOX™ Green (SYTOX ...
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bioRxiv - Biophysics 2023Quote: ... or 3 µM SytoxBlue Cell Death stain (Thermos Fisher Scientific, S34857). Pyroptosis kinetics measurements were performed by acquiring images before pyroptosis induction and afterwards with intervals of 15 min at a Spinning disk confocal microscope (CellVoyagerTM CQ1 Benchtop High ...
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bioRxiv - Neuroscience 2024Quote: Cell death was detected using Propidium iodide (PI, Thermo Fisher Scientific) or SYTOX™ Green (SYTOX ...
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bioRxiv - Microbiology 2024Quote: ... Vectors of sulfatase 1 and 2 tagged with myc were transfected using Lipofectamine 2000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Purified His-tagged proteins were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris Protein Gels, Invitrogen) and gels were stained with Quick Coomassie Stain (Generon) ...
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bioRxiv - Microbiology 2022Quote: ... His-tagged proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific Cat. Nr. 88222) according to manufacturer’s instructions (batch protocol) ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher Scientific cat.# 88221). After washing by wash buffer (25 mM Imidazole ...
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bioRxiv - Immunology 2023Quote: ... Filtered supernatants containing His-tagged proteins were passed slowly through HisPur Ni-NTA Resin (Thermo Fisher 88221) beads in columns ...
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bioRxiv - Biochemistry 2023Quote: ... The His tag was digested during 14h at 4°C with histidine-tagged 3C proteases (Thermo Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... His-tagged FH(44-511) was purified from the lysate using HisPurTM Cobalt Spin Columns (Thermo Fisher). Columns were washed using with buffer A (50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Synthetic Biology 2023Quote: All RBD antigens were His-tagged and purified using HisPur Ni-NTA resin (Thermo Fisher Scientific, 88222). Cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... Fms-like tyrosine kinase 3 ligand (Flt3- ligand; 100 ng/mL) and 1% penicillin-streptomycin (GIBCO, Grand Island, NY, USA), in the presence or absence of G-CSF at a dose of 100 ng/mL ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Genetics 2022Quote: ... BMP4 ligand (Gibco, #PHC9534) was added to cells at 100 ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant aFGF/FGF-1 and bFGF/FGF-2 were purchased from Thermo Fisher Scientific (13241-103 and PHG0024) ...
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bioRxiv - Neuroscience 2021Quote: ... Percent of cell death was determined by staining with 7 μM Hoechst 33342 and 2 μM propidium iodide (PI) (Invitrogen, USA). PI were diluted in warm neuronal culture medium and incubated for 5 min and then images were taken by a Zeiss microscope equipped with automated computer assisted software (Axiovision 4.6 ...
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bioRxiv - Microbiology 2020Quote: Mice engrafted with J-Lat cells were treated with recombinant human TNF-α (Cat. #PHC3011, Gibco), a potent and well-known LRA ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell death was determined using SYTOX dead cell stain sampler Kit (ThermoFisher Scientific, S34862) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: 250 μg of protein from HEK-293T cell lysates were incubated overnight with 2 μg of a 6x-His tag antibody (MA 1-135, Thermo Fisher) in the presence of a low–salt RIPA buffer (20mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human TNF-α a well established trigger of HIV-1 reactivation was purchased from Gibco. The PKC activator Bryostatin and Sodium Butyrate (NaBu ...
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bioRxiv - Cell Biology 2020Quote: ... 20 ng/ml recombinant human epidermal growth factor (Invitrogen, USA), 10 ng/ml human basic FGF (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... human recombinant Epidermal Growth Factor (5 ng/ml, ThermoFisher Scientific) and 0.15 mM CaCl₂ (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... coated with 5μg/ml human recombinant vitronectin (Life Technologies, #A14700) in mTesR1 (StemCell Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... 0.36 pM Recombinant Human Bone Morphogenetic Protein 4 (BMP4, Gibco), and 0.08 pM Activin A (Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... and human recombinant epidermal growth factor (EGF; Gibco 10450-013). All cells were cultured at 37 °C and 5% CO2.
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bioRxiv - Neuroscience 2020Quote: Glial-Derived Neurotrophic Factor (GDNF) Recombinant Human Protein (ThermoFisher, #PHC7044)
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bioRxiv - Neuroscience 2020Quote: Brain-Derived Neurotrophic Factor (BDNF) Recombinant Human Protein (ThermoFisher, #PHC7074)
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bioRxiv - Bioengineering 2020Quote: ... 12.5μg/ml Insulin human recombinant zinc solution (Thermo Fisher 1258014)] ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10 ng/mL recombinant human TRAIL (Life Technologies, PH1634) in serum-free medium for 4 hours ...
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bioRxiv - Neuroscience 2022Quote: ... 10 ng/ml recombinant human basic fibroblast growth factor (Invitrogen) (hiPSC medium) ...
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bioRxiv - Bioengineering 2022Quote: ... and pre-coated vitronectin recombinant human protein (ThermoFisher Scientific #A14700) at 0.5 μg/cm2.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Human paraoxonase1 (PON1) recombinant protein was purchased from Thermo Fisher Scientific Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Human recombinant TNFα was purchased from Invitrogen (Carlsbad, CA, USA). Puromycin was purchased from Funakoshi (Tokyo ...
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bioRxiv - Bioengineering 2021Quote: ... recombinant human oncostatin M (hOSM, 20 ng/ml, Gibco, PHC5015) and recombinant human insulin-like growth factor 1 (hIGF-1 ...
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bioRxiv - Physiology 2022Quote: ... and 5 ng/mL recombinant human EGF (Gibco Cat# PHG0311). Once IPEC-J2 reached confluence ...
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bioRxiv - Developmental Biology 2023Quote: ... wells were coated with Human Recombinant Laminin 511 (Fisher Scientific) in a 1:20 dilution in PBS with calcium and magnesium for 3 hours at 37 °C ...
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bioRxiv - Systems Biology 2024Quote: ... FGF Basic (aa 10 155) Recombinant Human Protein (Gibco #PHG0021), 1% Penicillin-Streptomycin Solution and 1% L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... They were cultured on human recombinant vitronectin (Thermo Fisher Scientific) coated plates in StemFlex medium (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... or human TGFβ1 recombinant protein (10ng/mL)(ThermoFisher Scientific, PHG9204) for 24 hours followed by addition of lipopolysaccharides (LPS ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for cell death using either LIVE/DEAD Fixable Aqua Dead Cell marker (Invitrogen) or GhostDye Violet 510 Viability Dye (TONBO Biosciences ...