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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Mumps virus lysate Isolate 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... virus was serially diluted in Vero maintenance media (MEM supplemented with 2% FBS and 1% each of GlutaMax (Gibco, Grand Island, NY), PenStrep ...
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bioRxiv - Neuroscience 2021Quote: ... the PureLink® RNA Mini Kit was used to isolate RNA with DNase digestion (Thermo Fisher Scientific 12183025). To convert RNA to cDNA ...
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bioRxiv - Immunology 2019Quote: ... were then infected with EV71 viral isolates at MOI 5 in serum-free Iscove’s Modified Dulbecco’s Medium (IMDM; Gibco) (50) ...
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bioRxiv - Microbiology 2020Quote: ... Drosten) and SARS-CoV (isolate HKU39849) were propagated on Vero cells in Opti-MEM I (1X) + GlutaMAX (Gibco), supplemented with penicillin (100 IU/mL ...
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bioRxiv - Molecular Biology 2020Quote: Whole genome sequencing of the three XDR-TB isolates was performed using Ion Torrent PGM platform (Life Technologies). Briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... The arrayed isolates were recovered in a 96 deep-well plate (Costar, Thermo Fisher Scientific, Waltham, MA, USA) by addition of 1 ml of appropriate growth (either LB or R2A ...
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bioRxiv - Microbiology 2019Quote: ... The DNA of isolates was extracted using the GeneJET Plant Genomic DNA Purification Mini Kit (Thermo Fisher Scientific) or DNeasy Plant Mini Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Complimentary DNA (cDNA) libraries were prepared from each RNA isolate with SuperScript III reverse transcriptase (ThermoFisher Scientific, Canada) and an anchored oligo-dT18 primer (Table 1) ...
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bioRxiv - Microbiology 2020Quote: Isolate stocks from −80°C storage were cultured on to CBA and supplemented with cefpodoxime (Fisher Scientific, USA) 10 μg discs for isolates not sensitive to cefotaxime during original sample isolation ...
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bioRxiv - Microbiology 2022Quote: ... using bacterial isolates cultured overnight in Dulbecco’s modified Eagle’s medium with low glucose (DMEM-LG; Invitrogen, Carlsbad, CA) at 37°C without aeration ...
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bioRxiv - Microbiology 2022Quote: ... Gentamicin susceptibility of each isolate was tested using 10μg gentamicin antimicrobial susceptibility discs (Thermo Scientific™ Oxoid™). Growth in iron-limited LB was verified by measuring OD600 (600nm ...
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bioRxiv - Neuroscience 2023Quote: Protein isolates from NPCs were analyzed on the Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). MS data analysis was carried out by using the Proteome Discoverer platform ...
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bioRxiv - Microbiology 2023Quote: DNA extractions for the initial 18 isolates were performed with a GeneJET Genomic DNA Purification Kit (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... ALI cultures were then dosed with 10µL of microbial isolate/vehicle (ultrapure water, Fisher Scientific, #AAJ71786AP, Hampton, NH). Microbial isolates were dosed at 107 CFUs ...
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bioRxiv - Cell Biology 2023Quote: Mitochondrial isolates (15 μg) were separated by electrophoresis using 3-12% NativePAGE gels (Life Technologies Australia, Mulgrave, Australia) to assess supercomplex formation using a 4 g/g digitonin/protein ratio ...
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bioRxiv - Genetics 2023Quote: ... we treated the total RNA isolates with DNase I using the DNA-free™ DNA Removal Kit (Invitrogen) following the manufacturer’s instructions for routine DNase digestion ...
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bioRxiv - Microbiology 2024Quote: ... Isolates of visually distinct colonies were then picked from the plate using a sterile calibrated loop (ThermoFisher Scientific), streaked onto blood agar (Hardy Diagnostics ...
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bioRxiv - Microbiology 2024Quote: ... aeruginosa (strain PA14) and all CF clinical isolates were grown in luria broth (LB, ThermoFisher Scientific, Waltham, MA) liquid cultures at 37°C with shaking at 225 rpm for 16 hours ...
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bioRxiv - Genomics 2022Quote: ... The lysate was then treated with 10 µl diluted (1:25) Rnase I (Thermo Fisher cat# AM2295) and 5 µl TurboDNase (Thermo Fisher cat# AM2239B001 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysate (1 mg per input) was incubated with mouse anti-HA magnetic beads (Thermo Scientific, #88836) at a ratio of 20:1 v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... the cell lysates were incubated with Myc monoclonal antibody (9E10) (MA-1-980, ThermoFisher Scientific, Waltham, MA) coupled to Protein G magnetic beads (88848 ...
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bioRxiv - Genetics 2020Quote: ... 400 μl of lysate was treated with 4 μl of DNase I (1 U/μl, Thermo Scientific) and 8 μl of RNase I (100 U/μl ...
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bioRxiv - Bioengineering 2022Quote: ... Protein lysates were separated by semi-dry western blot (anti-TNF, 1:1000, Invitrogen (14-7321-85); anti-GAPDH ...
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bioRxiv - Immunology 2023Quote: ... Lysate was diluted 1:3 in distilled water and digested in 0.2mg/ml proteinase-K (Invitrogen; 25530049) for 10 minutes at 55°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mg of lysate was immunopurified with 20 μL of M2 Anti-FLAG magnetic beads (Thermo Fisher) and FLAG tagged material eluted using an excess of FLAG peptide (Sino biological ...
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bioRxiv - Microbiology 2023Quote: ... The cell lysate-resin mixture was added to a disposable plastic column (1×5 cm) (Thermo Scientific) and allowed to settle ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of the peptide lysate was injected into a Vanquish Neo nanoHPLC system (Thermo Fisher Scientific). The peptide lysates were initially trapped on a C18-reverse phase trapping column (Acclaim PepMapTM 100 ...
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bioRxiv - Cancer Biology 2021Quote: ... lysates were partially digested with RNAseT1 (Ambion) (0.25U per mg of lysate 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate was treated with DNase (DNAfree, Ambion) and diluted with 400 μl of correction buffer (NP-40 ...
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bioRxiv - Neuroscience 2021Quote: ... Lysate was clarified by centrifugation (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... lysates were digested with RNaseI (Invitrogen, #AM2294), then layered onto a 1 M sucrose cushion in Footprinting Buffer plus 0.5 mM DTT and centrifuged in a Beckman Optima TLX Ultracentrifuge at 60,000 rpm for 1 hour at 4°C using a TLA100.3 rotor to isolate 80S ribosomes ...
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bioRxiv - Neuroscience 2020Quote: ... Lysate and sample buffer (Life Technologies, NP0008) were boiled ...
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bioRxiv - Cancer Biology 2023Quote: ... and lysates were treated with RNAseI (Ambion) before ultracentrifugation over a sucrose gradient using an SW-41Ti rotor at 222,200 x g for 2 hours at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Caspase-1 p20 and Actin levels in tissue lysates were detected by rabbit polyclonal anti-Caspase 1 p20 antibody (Thermo Fisher, Australia) and rabbit HRP conjugated anti-beta-Actin (Cell signalling ...
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bioRxiv - Microbiology 2021Quote: ... Each 10-μl RT-qPCR mixture consisted of 2.5 µL 4X TaqMan Fast Virus 1-Step Master Mix (Life Technologies, Carlsbad, CA, USA), 400 nM each primer ...
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bioRxiv - Microbiology 2020Quote: The TaqMan real-time RT-PCR assays were performed by using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, MA, USA). Each 20 μl reaction mix contained 5 μl of 4×Fast Virus 1-Step Master Mix ...
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bioRxiv - Pathology 2023Quote: ... RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (Life Technologies, Carlsbad, CA, USA) on the LC96 instrument (Roche ...
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bioRxiv - Microbiology 2023Quote: ... in a total volume of 25 µL containing 5 µL of template employing Taqman Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, USA). Oligonucleotide primer and probe sequences and thermocycling conditions are detailed in Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA concentrations of 30 samples were normalized to approximately 15 ng µl-1 and TSV was detected and quantified from 2 µl of sample using a TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems™) following the OIE recommended protocol and triplicate standards (mean R2 = 0.93 ± 0.06 ...
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bioRxiv - Bioengineering 2022Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...
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bioRxiv - Systems Biology 2023Quote: ... and yield was determined by quantitative real-time PCR using the TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems, Foster City, CA) and TaqMan Gene Expression Assay ...
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bioRxiv - Microbiology 2024Quote: ... A 10 µL RT–qPCR reaction to identify the RNA enteric viruses of interest contained 2.5 µL of fast virus 1-step master mix (4X) (Life Technologies, Carlsbad, CA, USA), 500 nM of each specific primers ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was harvested for virus titration and cells were lysed in Trizol (Invitrogen). MHV in the supernatant was quantified by standard plaque assay on L2 cells.
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bioRxiv - Cell Biology 2020Quote: ... HEK-293T cells used for virus production were cultured in IMDM (Life Technologies) supplemented with 20% IFS ...
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bioRxiv - Neuroscience 2020Quote: ... Virus-containing supernatant was harvested three days after transfection with Lipofectamine 2000 (Thermofisher). This supernatant was then cleared from cell debris by centrifugation at 3500 rpm for 15 min and filtered through a 0.45 µm filter (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... to wash the virus in Opti-MEM™ Medium (ThermoFisher Scientific, Darmstadt, Germany) and concentrate to a final volume of 150 μl.
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bioRxiv - Neuroscience 2020Quote: ... They were reprogrammed into iPSCs using the replication incompetent Sendaï virus kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The virus pellet was then re-suspended in PBS (Thermo Fisher Scientific, 10010015) containing 25 mM HEPES buffer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... For this the virus was diluted in 3 mL of 199 medium (Gibco). After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...