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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Mumps virus lysate Isolate 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... ten-fold dilutions of virus were prepared in diluent medium (Opti-MEM (Invitrogen) with 2% FBS (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-Rex 293 HEK cells (Invitrogen) which repress antigen expression during virus propagation ...
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bioRxiv - Immunology 2021Quote: ... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...
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bioRxiv - Genomics 2021Quote: ... hiPSCs were generated from primary fibroblasts using Sendai virus reprogramming kit (ThermoFisher Scientific), yielding 2-3 different clonal lines for each individual ...
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bioRxiv - Cancer Biology 2022Quote: ... packaging cell line for virus production were cultivated in DMEM high glucose (Gibco), supplemented with 10% heat-inactivated fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
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bioRxiv - Immunology 2022Quote: ... and Toledo virus was conjugated with DMSO-dissolved AF647–N-hydroxysuccinimide ester (Invitrogen) with constant agitation at room temperature for 1 hour ...
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bioRxiv - Microbiology 2022Quote: Purified virus particles were incubated with different concentrations of BS3 cosslinkers (ThermoFisher Scientific) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml, Gibco) 24h later ...
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bioRxiv - Neuroscience 2022Quote: ... PBMCs were expanded and reprogrammed using Sendai virus vector (CytoTune-iPS 2.0, Invitrogen) using a feeder-free system (Sommer et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (ThermoFisher Scientific). Real-time PCR was performed using TaqMan assays (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... RNA from virus stocks was purified using TRIzol Reagent (Thermo Fisher Scientific, #15596026). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The virus-containing media were filtered through a 0.45 µm filter (ThermoFisher Scientific) and mixed with fresh media at a 1:1 ratio before adding to pre-seeded target cells ...
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bioRxiv - Immunology 2024Quote: ... the media was changed to virus production medium containing Opti-MEM I (Gibco) + 5 % FBS + 1:100 Glutamax + 1:100 MEM-NEAA (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Nuclease treatment of virus stocks was performed at room temperature by DNase (Ambion), RNase (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...
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bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...
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bioRxiv - Biochemistry 2024Quote: ... cells were given virus production media (DMEM supplemented with 20mM HEPES (Gibco; 15630080) and 30% FBS) ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was prepared using Moloney Murine Leukemia Virus reverse transcriptase (Thermo Fisher Scientific) with Random Hexamers (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... or immunoprecipitated type I and II PKA holoenzymes from iPSC-CMs whole cell lysate or whole heart lysates from Stk25+/+ or Stk25-/- mice following the manufacturer’s protocol (EIAPKA, Invitrogen). Briefly ...
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bioRxiv - Immunology 2019Quote: ... diluted whole cell lysate and phosphoenriched lysates were processed for SDS-PAGE with NuPage LDS Sample buffer (Life Technologies) and 100mM dithriothreitol (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... Both mock transfected lysate and STING-GFP transfected lysate were separately incubated with antibodies against GFP (Life Technologies A11122). All antibodies were incubated overnight at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2023Quote: Each lysate was treated with 0.3 U/μL (1.5 µL for 500 µl of lysate) of RNase I (ThermoFisher # AM2294) for 40 min at 20°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Most protein lysates were separated by running 30-100 μg of lysate on 10% Bis-Tris NuPage gels (Invitrogen) and subsequently ...
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bioRxiv - Microbiology 2022Quote: ... the culture supernatant was recovered to isolate RNA using MagMax Viral/Pathogen Nucleic Acid Isolation kit (Thermo Fisher Scientific) and quantify SARS-CoV-2 RNA by real time RT-PCR with a one-step qRT-PCR kit (THUNDERBIRD Probe One-step qRT-PCR kit ...
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bioRxiv - Microbiology 2020Quote: ... the sequence from isolate H77C (GenBank accession number AF011751; residues 384–661) was cloned into the pSecTag2 vector (Invitrogen), transfected with 293fectin into FreeStyle HEK293-F cells (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... while isolates intended for robot performed susceptibility testing were subcultured in a flat-bottom 96-well plate (Nunc, 167008), each well filled with 220 μL (allowing for overnight evaporation of roughly 20 μL ...
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bioRxiv - Microbiology 2021Quote: ... The isolates of bacteria were stained with a NucBlue Live ReadyProbes reagent (Thermo Fisher Scientific Inc., Waltham, MA, USA). During the infection ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 isolates (Human/NL/Lelystad/2020) were propagated in Vero E6 cells (ATCC) in Eagle’s minimal essential medium (Gibco) supplemented with 1% L-glutamine ...
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bioRxiv - Microbiology 2020Quote: ... frugiperda) IPLB-Sf21-AE clonal isolate 9 (Sf9) insect cells were cultured at 27°C in Grace’s medium (Gibco) containing 10% fetal bovine serum (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... Approximately 10μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification ...
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bioRxiv - Genetics 2023Quote: ... Antimicrobial susceptibility of Campylobacter isolates was determined using the Sensititre semi-automated system (Thermo Fisher Scientific, Kansas City, KS) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentrations of the EV isolates were determined using the PierceTM BCA protein assay kit (Thermo Fisher Scientific) and 10 ug aliquots were prepared and stored at -80°C until further analysis.
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bioRxiv - Genetics 2022Quote: ... the chromatin lysates were precleared with a 1:1 mix of protein A/G Dynabead magnetic beads (Thermo Fisher, cat# 10001D and 10003D) for 1 hour at 4°C and then incubated overnight at 4°C with 1:1 mix of protein A/G Dynabead magnetic beads conjugated to H3K9me3 antibody (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... Absorbance readings (260 nm) of 1:10 dilutions of the lysate were performed with a Nanodrop (Thermo Scientific). The amount of extract needed for 30 A260 units for each lysate was aliquoted ...
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bioRxiv - Cell Biology 2021Quote: ... cell lysates for qPCR were prepared using the Cells-to-CT 1-Step Taqman Kit (Invitrogen, cat# A25603) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... 200 μg (100 μl) of FSBA-treated cell lysate was incubated with 1 μg of kinase (Life Technologies) and 1 mM γ[18O4]-ATP (Cambridge Isotope Laboratory ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was diluted 1:3 in distilled water and digested in 0.2 mg/ml proteinase-K (Invitrogen; 25530049) for 10 min at 55 °C ...
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bioRxiv - Microbiology 2020Quote: Cell lysates were collected in 1% Triton X-100 buffer and quantified by Bradford protein assay (Thermo Scientific). Proteins were separated by SDS-PAGE and transferred to PVDF membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg of cell lysate was immunoprecipitated with 2 μg of anti-PODXL antibody (3D3, Thermo Fisher Scientific) overnight at 4ºC with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... 1% SDS-containing lysates were added to Sera-Mag Beads (Thermo Scientific, #4515-2105-050250, 6515-2105-050250) in 10 µl 15% formic acid and 30 µl of ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... The cell lysate was incubated with the 1-Step p-nitrophenyl phosphate disodium salt (PNPP) substrate (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 1% SDS-containing lysates were added to Sera-Mag Beads (Thermo Scientific, #4515-2105-050250, 6515-2105-050250) in 10 µl 1 5% formic acid and 30 µl of ethanol ...
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bioRxiv - Microbiology 2019Quote: ... The number of JEV RNA copies present in BBB-crossing samples was determined by RT-qPCR using TaqMan® Fast Virus 1-Step Master Mix kit (Applied Biosystems®, 4444432) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... MHV-A59 was quantified by RT-qPCR by amplifying a fraction of its ORF5 gene for the structural protein M.15,16 The TaqMan™ Fast Virus 1-Step Master Mix Kit (Thermo Fisher Scientific Inc., 4444432) was used for RT-qPCR ...
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bioRxiv - Microbiology 2021Quote: ... cDNA were synthesized from 100 pg of RNA and quantified using the TaqMan® Fast Virus 1-Step Master Mix (Thermofisher, Waltham, Massachusetts, USA) following manufacter’s instructions with appropriate oligonucleotides and Taqman probes (Table S1) ...
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bioRxiv - Immunology 2020Quote: Subgenomic RT-qPCR was performed on the QuantStudio™ 7 Flex Real-Time PCR System using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with forward primer ...
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bioRxiv - Microbiology 2023Quote: ... Isolated RNA was subjected to one-step RTqPCR analysis as described using primers specific for the viral genome encoding for the RNA-dependent RNA polymerase (RdRp)23 and for E24 genes with TaqMan fast virus 1-step master mix (ThermoFisher Scientific, catalogue number 4444432) using AriaMx instrumentation (Agilent ...