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Citations for Thermo Fisher :
401 - 450 of 1551 citations for Echovirus Antigen Recombinant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
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bioRxiv - Evolutionary Biology 2022Quote: ... were transfected with recombinant bacmids using Cellfectin II reagent (Gibco-Thermo Fisher Scientific™ ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with recombinant murine EGF (100 ng mL-1, Gibco), recombinant human R-spondin 1 (200 ng mL-1 ...
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bioRxiv - Physiology 2022Quote: Recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transient transfection ...
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bioRxiv - Microbiology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore) ...
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bioRxiv - Developmental Biology 2023Quote: ... wells were coated with Human Recombinant Laminin 511 (Fisher Scientific) in a 1:20 dilution in PBS with calcium and magnesium for 3 hours at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... 200 units/mL RNaseOUT™ Recombinant Ribonuclease Inhibitor (#10777019; ThermoFisher), and 1X cOmplete ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.15 μL recombinant RNase Inhibitor (40 U/μL, Thermo Fisher); 0.06 μL Dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2023Quote: ... 80 U of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher), and 25 μl of T7 RNA polymerase (lab made) ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µg (60 pmol) of recombinant Cas9 protein (A36498, Invitrogen) was incubated with 180 pmol of sgRNA in a 20 µL reaction for 20 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: ... 0.5 μl of RNaseOUT Recombinant Ribonuclease Inhibitor (ThermoFisher, 10777-019) to the hexamer annealed RNA ...
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bioRxiv - Systems Biology 2024Quote: ... FGF Basic (aa 10 155) Recombinant Human Protein (Gibco #PHG0021), 1% Penicillin-Streptomycin Solution and 1% L-glutamine ...
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bioRxiv - Biophysics 2023Quote: ... Tetravalent recombinant (wild-type) streptavidin was procured from Thermo Scientific. Plasmids encoding SBD2 (T369C/S451C ...
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bioRxiv - Microbiology 2023Quote: ... They were cultured on human recombinant vitronectin (Thermo Fisher Scientific) coated plates in StemFlex medium (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 ng/mL recombinant mouse interleukin-3 (Gibco PMC0031).
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bioRxiv - Immunology 2024Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher, Cat. #A14635) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... or human TGFβ1 recombinant protein (10ng/mL)(ThermoFisher Scientific, PHG9204) for 24 hours followed by addition of lipopolysaccharides (LPS ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 50 ng/ml mouse recombinant EGF (Invitrogen, PMG8043). The organoids were trypsinized with 0.05% Trypsin-EDTA (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... and FL Spike trimers from hCoVs polypeptide antigens) were purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer (20 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... Antigen recognition was performed by incubating primary antibody in a humidified chamber for different times (Phalloidin, Invitrogen, A12381 ...
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bioRxiv - Biochemistry 2019Quote: ... a rabbit polyclonal antibody specific to soluble tachyzoite antigen (STAG) directly conjugated to FITC (PA1-7253, Invitrogen) was diluted 1:300 in TBS-T (0.1% Tween20 ...
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bioRxiv - Cell Biology 2020Quote: ... The antiserum was affinity purified using the antigen coupled to Dyna M-280 Streptavidin beads (Life Technologies).
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bioRxiv - Biochemistry 2020Quote: ... antigens or capture antibodies were coated in PBS overnight onto 384 microwell plates (Maxisorp, Thermo Fisher Scientific), washed 5 times with PBST (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... the antigen was detected using chemiluminescence Western blotting detection reagents (Pierce ECL Western Blotting Substrate, Thermo Fisher Scientific Inc. ...
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bioRxiv - Pathology 2022Quote: ... Antigen antibody complexes were visualised with anti-mouse Alexa 488- or 594-conjugated antibody (Thermo Fisher Scientific) or Immpress anti-mouse/rabbit coupled with tyramide amplification kit (PerkinElmer ...
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bioRxiv - Plant Biology 2020Quote: ... The antibody-antigen complex was detected by using the SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Scientific) and the FujiFilm LAS-3000 or Amersham imager 600 analyzers.
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bioRxiv - Neuroscience 2019Quote: ... antibody/antigen mixture was incubated with Dynabeads M-280 sheep anti-rabbit IgG (Thermo Fisher Scientific, 11203D) or Dynabeads M-280 sheep anti-mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... the antigen was detected using chemiluminescence Western blotting detection reagents (Pierce ECL Western Blotting Substrate, Thermo Fisher Scientific Inc. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by binding of antigen antibody complexes to protein A/G magnetic beads (Thermo scientific, Rockford, USA) for 1 h ...
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bioRxiv - Immunology 2020Quote: ... while non-Avi tagged antigens were biotinylated chemically using EZ-Link Sulfo-NHS-Biotin (Thermo Fisher, A39256). VH and VL sequences of SARS-CoV-2 control antibodies (COV2-2196 ...
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bioRxiv - Cancer Biology 2021Quote: ... epithelial membrane antigen (EMA; clone GP1.4, mouse monoclonal, dilution 1:1000, Thermo Fisher Scientific, Fremont, CA, USA), Sry-related HMG-BOX gene 10 (SOX10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antigen detection was accomplished with goat-ant-rabbit Cy3 secondary and chicken-anti-mouse Cy5 secondary (ThermoFisher, Waltham ...
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bioRxiv - Immunology 2020Quote: Antigen specific antibodies were depleted in a bead-based approach using Ni-NTA Magnetic beads (Thermo Scientific) as described (62) ...
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bioRxiv - Neuroscience 2022Quote: ... All sections were subjected to heat-mediated antigen retrieval in 10 mM sodium citrate (pH6.5; Fisher Scientific) in distilled water for 20 minutes at 80°C prior to washing 3 times in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibody/antigen complexes were recovered with Dynabeads M-280 sheep anti-rabbit IgG (Thermo Fisher Scientific, 11203D). Beads with DNA fragments were washed once with RIPA buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Protein content of the antigen was determined with Coomassie (Bradford) Protein assay kit (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and SV40 large T antigen (20 ng) suspended in Opti-MEM I Reduced-Serum Medium (ThermoFisher Scientific), mixed with 293fectin (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Protein antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). OSP and LPS antigens were modified by 4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium and conjugated to Luminex Magplex carboxylated beads ...
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bioRxiv - Synthetic Biology 2023Quote: All RBD antigens were His-tagged and purified using HisPur Ni-NTA resin (Thermo Fisher Scientific, 88222). Cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed by the use of Lab Vision PT Module (Thermo Fisher Scientific, Waltham, MA), in 10 mM citrate pH 6 ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-induced (98°C) antigen retrieval was performed with a citrate buffer (pH 6.0) (Thermo Fisher Scientific), and slides were incubated with Triton X-100 0.25% in PBS for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... with 0.1 ng/mL human recombinant epidermal growth factor (EGF; Gibco) and 0.05 mg/ml bovine pituitary extract (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... and human recombinant insulin (2.5 µg/mL, Thermo Fisher Scientific, 12585014). From Day 1 to 11 ...
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bioRxiv - Cell Biology 2020Quote: ... Ki-67 recombinant rabbit monoclonal antibody (SP6) (Thermofisher, Cat. #: MA5-14520), Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Genetics 2021Quote: ... and Mouse M-CSF Recombinant Protein were from Invitrogen (Carlsbad, CA). Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant soluble ACE2 was coated on NUNC Maxisorp plates (Thermo Scientific) at 1µg/well at RT for 3 h ...
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bioRxiv - Biochemistry 2020Quote: ... Full-length recombinant human LRRK2[G2019S] was purchased from Invitrogen (#A15202). GZD-824 was purchased from Cayman Chemical (#21508 ...