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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for ETS Related Transcription Factor Elf 3 ELF3 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... T cells were fixed and permeabilized using the eBioscience FoxP3/Transcription Factor Staining Buffer Set (Invitrogen, #00-5523-00) following manufacturer’s instructions and stained for intracellular cytokines for 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... for 20 min at room temperature before fixation/permeabilization with the eBioscience Foxp3 / Transcription Factor Staining Buffer Set (Invitrogen) and intracellular staining for 40 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized using FoxP3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific Cat# 00-5523-00) and stained with anti-mouse antibodies targeting intracellular markers according to standard protocols.
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bioRxiv - Cancer Biology 2022Quote: ... Fixation was performed with 4% paraformaldehyde or Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific; 00-5523-00) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cell fixation and permeabilization was performed using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cell fixation and permeabilization was performed using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were permeabilized with 1X permeabilization buffer (PB) (kit Foxp3/Transcription Factor Staining Buffer kit, Thermo Fisher, 00-5523) for 30 min RT ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining was performed using the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Invitrogen, Cat. 00-5523-00) at room temperature for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... the samples were fixed with eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent (Thermo Fisher Scientific, USA) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining was performed by fixing and permeabilizing with the eBioscience Foxp3 / Transcription Factor Staining Set (Thermo Fisher Scientific). EdU positive cells were stained with Click-iT Plus EdU AlexaFluor 488 Flow Cytometry Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed and permeabilized using the FOXP3/Transcription factor staining buffer kit (00-5523-00; Thermo Fisher Scientific) following manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized for 30 min with eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) before staining with the following antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... and Foxp3 was performed using the eBioscience Foxp3 transcription factor fixation/permeabilization kit per manufacturer’s instructions (Thermo Fisher Scientific). Data were acquired on a FACS Symphony A5 (BD Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were washed with PBS and permeabilized using the Foxp3 Transcription Factor Staining Buffer Set (Invitrogen, 00-5523-00) for 1 h in the dark at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... HRP-conjugated secondary antibodies diluted by 1:10000 (HRP-conjugated Goat-anti-Mouse IgG secondary antibody for GFP, Invitrogen, USA ...
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bioRxiv - Microbiology 2020Quote: ... rabbit α-RSV polyclonal antibody and HRP-conjugated secondary antibodies (Invitrogen). RSV antibodies were generous gifts from Rebecca Craven (Penn State College of Medicine) ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were washed 3×10 minutes in PBS-T and incubated with secondary antibody (goat anti-rabbit IgG HRP conjugate, Invitrogen, G21234) diluted in 1% milk +BSA (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were washed 3 times in TBS-T and incubated with secondary antibody (Goat anti-rabbit IgG HRP conjugate, Invitrogen, G21234) for 1 hour at room temp ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were blocked again and incubated with 2-3 drops of an anti-mouse poly-HRP-conjugated secondary antibody from the Invitrogen Tyramide SuperBoost secondary staining kit (ThermoFisher, B40912). Samples were rinsed three times with 1X PBS and the tyramide working solution conjugated to AlexaFluor 488 was added and allowed to react for 20 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibodies anti-human-HRP and goat anti-mouse-HRP were purchased from Thermofisher Scientific and Vector Labs ...
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bioRxiv - Plant Biology 2022Quote: ... BIK1 was detected in Western blots assays using HA-HRP antibody (Invitrogen, 26183-HRP)) and its phosphorylation was quantified by calculating the ratio between the intensity of the shifted upper band of phosphorylated BIK1 (pBIK1 ...
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bioRxiv - Neuroscience 2023Quote: ... Horseradish peroxidase (HRP) secondary antibodies (goat anti-mouse HRP conjugated (1:10,000, 626820, Invitrogen) were incubated for 2 h at RT and the proteins were detected with Supersignal West Pico (34580 ...
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bioRxiv - Cell Biology 2024Quote: ... and HRP-conjugated anti-actin antibody (1:5000 dilution, ThermoFisher, ref: MA5-15739-HRP) in 5% non-fat milk in PBS-T ...
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bioRxiv - Microbiology 2022Quote: ... These RNAs were 35 nucleotides long and they were prepared by in vitro transcription using a DNA template (5’-CAGTAATACGACTCACTATAGGGGAAGCGGGCATGCGGCCAGCCATAGCCGATCA-3’) and the TranscriptAid T7 high-yield transcription kit (Thermo Scientific). The reaction was performed in a 50-μl mixture containing 1× TranscriptAid reaction buffer ...
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bioRxiv - Genetics 2020Quote: ... abdomens of control flies and flies with CRISPR/Cas9 generated GFP-tagged GR8A were dissected and immunostained as previously described (Lu et al., 2012; Zheng et al., 2014) using a Rabbit anti-GFP antibody (1:1000; A-11122, Thermo Fisher Scientific) followed by AlexaFluor 488 anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Genetics 2022Quote: ... abdomens of control flies and flies with CRISPR/Cas9 generated GFP-tagged GR8A were dissected and immunostained as previously described (Lu et al., 2012; Zheng et al., 2014) using a Rabbit anti-GFP antibody (1:1000; A-11122, Thermo Fisher Scientific) followed by AlexaFluor 488 anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Immunology 2022Quote: ChIP-Seq experiments were performed as previously described (Li et al., 2017; Lin et al., 2012) using the following antibodies: anti-STAT5B (Invitrogen 13-5300), anti-H3K27Ac (Diagenode) ...
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bioRxiv - Neuroscience 2022Quote: ... Tol2 transposase mRNA was transcribed from linearized pCS2FA-transposase (Kwan et al., 2007) with the mMESSAGE mMACHINE sp6 transcription kit (Invitrogen). The resulting mRNA was co-injected (20 ng/µL ...
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bioRxiv - Genetics 2022Quote: For reverse transcriptase (RT)-PCR analyses, reverse transcription reactions were performed as previously described (Carninci et al., 1998) using SuperscriptTM III (Invitrogen). The synthesized cDNA was then used as a template in standard PCRs (Sambrook and Russell ...
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bioRxiv - Cancer Biology 2020Quote: ... Cas9 mRNA was produced by in vitro transcription from a pCS2 Cas9 vector (Jao et al., 2013) using mMESSAGE mMACHINE SP6 kit (Invitrogen). The satb2 gRNA 5’-GGATGGGCAGGGGGTTCCAG-3’ was generated following established methods (Gagnon et al. ...
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bioRxiv - Cell Biology 2023Quote: Fluorescently labeled vg1 LE RNA (Gautreau et. al., 1997) was generated from linearized pSP73-2x135 using the MEGAscript T7 transcription kit (Ambion) in the presence of 250 nM CyTM5-UTP ...
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bioRxiv - Developmental Biology 2020Quote: ... Fgfrb_fwd 5’-AAACGCGAAAAGACCCTGATAGC-3’ and Fgfrb_rev 5’-GGACAGCGGGGACGTCAG-3’ Antisense probe was synthesized by in vitro transcription (MEGAScript Kit; Ambion) driven by T7 RNA polymerase with DIG incorporation (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with the recommended dilution of HRP-conjugated secondary antibody (goat anti-rabbit IgG HRP 1:10000, Invitrogen; goat anti-mouse IgG HRP 1:10000, Invitrogen) at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with an appropriate HRP-conjugated secondary antibody (IgG Fc Goat anti-Rabbit, HRP, Invitrogen 31463; IgG Fc Rabbit anti-Mouse, HRP, Invitrogen 31455 ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-rabbit HRP antibody (Invitrogen, cat#656120), was diluted 1:10,000 in PBS containing 0.1% v/v Tween 20 and incubated for 1 hour at room tempterature ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibody HRP-anti-Rabbit (Thermo Fisher Scientific) was diluted 1:50,000 and signal was detected using Supersignal west femto substrate (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... HRP conjugated rec-Protein G antibodies (Invitrogen, 101223) were diluted (diluent mixture described above ...
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bioRxiv - Plant Biology 2021Quote: ... we used anti-V5 antibody HRP-conjugated (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... or HRP-conjugated secondary antibodies (Thermo Fisher Scientific) and washed again with PBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... and detected by HRP-conjugated secondary antibodies (Invitrogen; shaken in PBS ...
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bioRxiv - Immunology 2021Quote: ... followed by HRP-conjugated anti-rabbit antibody (Invitrogen) and incubated with TrueBlue substrate (Sera Care) ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary antibodies were purchased from Invitrogen. Chemiluminescence signals were obtained upon incubation with ECL™ Prime Western Blotting Detection Reagent (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in HRP secondary antibodies (Thermo Fisher Scientific) for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary HRP antibodies were purchased from Thermo Fisher. Unedited western blots provided in the Source Data File ...
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bioRxiv - Microbiology 2024Quote: ... and the appropriate HRP-conjugated secondary antibodies (Invitrogen).
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... A goat-anti mouse HRP conjugated antibody (Invitrogen) was diluted 1:1000 in TBS with Tween-20 ...
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bioRxiv - Developmental Biology 2023Quote: ... Horseradish peroxidase (HRP)–conjugated secondary IgG antibodies (Invitrogen, #31470 ...
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bioRxiv - Neuroscience 2023Quote: ... and HRP secondary antibody (REF32430, 1:1000, Invitrogen). The images were taken by ImageQuant LAS 4000mini (cytiva ...
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bioRxiv - Developmental Biology 2022Quote: ... HRP-conjugated secondary antibodies (Thermo Fisher Scientific, #31431) and ECL reagents (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... HRP-labeled rabbit anti-IgG antibody (Thermo Scientific) was the secondary antibody ...