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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for ETS Related Transcription Factor Elf 3 ELF3 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Primary antibodies were probed with anti-mouse HRP conjugated antibodies (Thermo Fisher).
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were then detected using horseradish peroxidase (HRP) conjugated antibody (Invitrogen) at 1:5,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were detected with HRP-conjugated secondary antibodies (Pierce, 1:5000) and chemiluminescent HRP substrates (Supersignal West Pico PLUS, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody HRP conjugated (31460) and goat anti-mouse IgG (H+L) secondary antibody HRP conjugated (31430) were from Thermo Scientific and rabbit anti-ATP13A3 antibody (HPA029471 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then incubated for 2h at room temperature with HRP-coupled secondary antibodies (anti-rabbit HRP, Proteintech; anti-mouse HRP, Thermofisher Scientific) diluted from 1/1000 to 1/10000 in blocking solution ...
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bioRxiv - Genomics 2021Quote: ... and Jurkat related cell lines are cultured in RPMI supplemented with 10% FBS (Invitrogen) and 1X pen/strep ...
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bioRxiv - Immunology 2020Quote: ... Confocal related reagents included antigen retrieval buffer low pH (cat. 00-4955-58, Invitrogen), donkey serum (cat ...
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bioRxiv - Microbiology 2024Quote: ... retinoid-related orphan receptor (RORγ; B2D, catalog no. 61-6981-82, Thermo Fisher Scientific), T-bet (4B10 ...
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bioRxiv - Genomics 2020Quote: ... cells were washed twice in ice cold staining Buffer and fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (ThermoFisher) per manufacturer’s recommendation ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice in Maxpar staining buffer followed by fixation and permeabilization using the eBioscience FoxP3/transcription factor staining kit (Thermofisher Scientific) for intracellular staining and barcode labeling ...
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bioRxiv - Immunology 2021Quote: ... samples were centrifuged again for 10 min at 14,000 x g, resuspended in permeabilization puffer (FoxP3 Transcription Factor Staining Buffer Set, eBioscience™) reconstituted with 10 % Normal Goat Serum (NGS, ThermoFisher) and stained with primary antibodies against Gephyrin (#ab136343 ...
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bioRxiv - Immunology 2022Quote: ... Staining for intracellular Notch1-IC was performed using fixation and permeabilization with Foxp3 transcription factor staining kit (Invitrogen; 00-5523-00). Intracellular staining was performed at room temperature in BD Foxp3 permeabilization buffer.
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bioRxiv - Pathology 2022Quote: ... in 1X Perm buffer (eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set, ThermoFisher, #00-5523-00, in the dark, 45min., RT). Cells were washed in 1X Perm buffer (350g ...
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bioRxiv - Pathology 2022Quote: ... RT) washed in 1X Perm buffer (eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set, ThermoFisher, #00-5523-00, 350g, 5min., RT) and incubated with anti-Ter119 (4µg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... The transcripts of lysosome biogenesis genes and their transcription factors were amplified from the cDNA in QuantStudio 6 Flex real-time PCR system (Applied Biosystems). As a control 18S rRNA or β-Actin were amplified along with the lysosomal genes ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was performed at room temperature using the eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher Scientific, Waltham, MA) as directed by the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... and then fixation and permeabilization using the eBioscience Foxp3/Transcription Factor Staining Buffer Set according to manufacturer’s instructions (Thermo Fisher Scientific). Antibodies used are listed in Table S3 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were then fixed and permeabilized using Invitrogen’s Transcription Factor Staining Buffer Set (Thermo Fisher Scientific; Cat. No.: 00-5523-00) according to manufacturer’s instructions and stained with Nur77 (12.14 ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed for 1 h at 4°C using a FOXP3/Transcription Factor Staining Buffer Set (Invitrogen, for FOXP3 detection) or 15 min at room temperature using a 1.85% formaldehyde solution in PBS (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: BAL and NT samples were stained in 96-wells plates using the FoxP3 / Transcription factor staining buffer set (eBioscience, Thermo Fisher). Cells were stained with α-CD4-APC (02 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed in FACS buffer and fixed and permeabilized with the Foxp3 / Transcription Factor Staining Buffer Set (00-5523-00, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed once with FACS buffer and subsequently fixed for 30 minutes with fixation/permeabilisation buffer of eBioscience Foxp3/transcription factor staining buffer set (ThermoFisher Scientific). They were then washed once with the permeabilisation buffer of the staining buffer set and stained with anti-Tax-AF647 (clone LT-4 ...
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bioRxiv - Immunology 2024Quote: ... Intranuclear and intracellular marker were stained after fixation and permeabilization of cells with the FoxP3/Transcription Factor staining buffer set (Thermo Fisher) or Cytofix/Cytoperm (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... cells were first surface stained as described above and fixed (eBioscience Transcription Factor Staining Set; Thermo Fisher Scientific, Waltham, MA, USA) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Parallel co-cultures were kept for one week to assess expression of FOXP3 and HELIOS (Stability) by intracellular staining using the FOXP3/Transcription Factor Staining Buffer Set (eBioscience, ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cells that received an intracellular stain were fixed and permeabilized using the FOXP3/Transcription Factor staining buffer set (Thermo Fisher Scientific). Flow cytometry data were collected on the LSRFortessa X-20 ...
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bioRxiv - Microbiology 2022Quote: ... Synthetic cDNA encoding a self-cleaving polyprotein Bcl-6.t2A.Blc-xL to express the germinal center B cell-associated transcription factors Bcl-6 and Bcl-xL was synthesized (B6L; Integrated DNA Technologies) and cloned in pENTR/D-TOPO (Thermo Fisher), generating pENTR.B6L (35 ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 40 min at 4°C using the eBioscience™ Foxp3/Transcription Factor Fixation kit (Thermo Fisher Scientific) and permeabilized using the kit’s permeabilization buffer ...
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bioRxiv - Immunology 2023Quote: ... the cells were fixed/permeabilized overnight at 4°C in dark using eBioscience FOXP3 transcription factor staining buffer set (cat. #00-5523-00; Thermo Fisher) and FOXP3 monoclonal antibody (1:25 dilution ...
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bioRxiv - Immunology 2023Quote: ... surface-labelled cell suspensions were fixed using eBioscience Foxp3/Transcription Factor Staining Buffer Set or eBioscience IC Fixation Buffer (both from Thermo Fisher). Cell suspensions were stained with following antibodies at 4°C for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then fixed and permeabilized using FOXP3 Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, Catalog no. 00-5523-00) and stained for intracellular markers RORψt anti-mouse PE-CF594 (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... All cells were then fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (00-5523-00, ThermoFisher), as per manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... Fixation and permealization was done using the eBioscience Foxp3/Transcription Factor Staining buffer set (ThermoFisher Scientific, catalogue no. 00-5523-00). Cells were acquired on BD FACSymphony A5 SE and Cytek Aurora and analyzed in FlowJo (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... Cells were fixed and permeabilized with the eBioscience™ FOXP3/Transcription Factor Staining Buffer Set (Invitrogen; Cat. No. 00-5523-00), followed by intracellular staining with fluorochrome-conjugated antibodies ...
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bioRxiv - Immunology 2024Quote: ... CD45R/B220 BV510) and then fixed and permeabilized using the eBioscience Foxp3 / Transcription Factor Staining Buffer Set (Invitrogen #00-5523-00) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... for 30 min at 4 °C prior to fixation and permeabilization (FOXP3/Transcription Factor Staining Buffer Set, 00– 5523, ThermoFisher Scientific) for 30 min at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... For intracellular staining cells were fixed and permeabilized using the eBioscience Foxp3 Transcription Factor Staining Buffer Set (Cat# 00-5523-00, ThermoFisher Scientific) followed by intracellular staining with the desired antibody overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Intracellular marker staining was performed using the eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, #00-5523-00). Data acquisition was conducted using an Attune NxT Flow Cytometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Annexin V-Alexa Fluor488 (used at 1 μg/mL) and eBioscience™ FOXP3/Transcription factor staining buffer set (Cat.00-5523-00) were from Invitrogen. Phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: U2OS 2-6-3 cells (Shanbhag et al., 2010) were cultured in McCoy’s 5A (Modified) Medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were detected with HRP-conjugated secondary antibodies (rabbit IgG-, Protein A-, or mouse IgG-conjugated with HRP, Thermo Fisher Scientific) and ECL detection reagent (Amersham) ...
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bioRxiv - Genomics 2023Quote: ... blots were washed thrice with 1× TBS-T for 10 minutes each and incubated with HRP conjugated secondary antibody (anti-Rabbit HRP Invitrogen, #32460; 1:10,000 or anti-Mouse HRP Invitrogen, #32430; 1:10,000) prepared in 1×TBS-T for 1 hour at RT on the rocker ...
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bioRxiv - Microbiology 2020Quote: ... or goat anti-hamster IgG-HRP conjugated antibody (Invitrogen) diluted (1:3,000 ...
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bioRxiv - Plant Biology 2020Quote: ... and goat anti-mouse-HRP antibodies (A-10668) (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: ... horseradish peroxidase-(HRP) conjugated secondary antibody (Novex, Life technologies) at 1 ...
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bioRxiv - Biochemistry 2020Quote: ... A goat-anti rabbit HRP conjugated secondary antibody (Invitrogen) was incubated using a 1:2500 dilution for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... and a goat anti-mouse-HRP secondary antibody (Invitrogen), and developed using the fluorescein substrate from the TSA Plus kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary horseradish peroxidase (HRP) antibodies were obtained from Invitrogen. Pierce ECL Chemiluminescence kit (Thermo Fisher scientific #32106 ...
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bioRxiv - Cancer Biology 2021Quote: ... HRP-conjugated secondary antibody was purchased from Thermo Fisher, while IRDye infrared secondary antibodies were purchased from Mandel Scientific.
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bioRxiv - Neuroscience 2022Quote: ... an HRP-conjugated goat anti-human secondary antibody (ThermoFisher) was added ...