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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Diethyl 2 chlorothiazol 5 yl methylphosphonate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The membrane was then coated with Rat-tail collagen I (5 μg cm-2, Gibco) for 4 hours and rinses with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and loaded with 5 μM Rhod-2 AM (R1244, Invitrogen) in HBSS without calcium/magnesium for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Digestion was quenched by adding PBS supplemented with 2% FCS and 5 mM EDTA (Gibco). Cell suspensions were obtained after filtration over the 70-μm cell strainer ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were detached after 2 or 5 days of co-culture with StemPro Accutase (Thermofisher), experimental duplicates were performed for each timepoint and each condition ...
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bioRxiv - Neuroscience 2020Quote: ... claudin-5 (1 μg/ml) and occludin (2 μg/ml; all from Thermo Fisher Scientific). After incubation with primary antibodies membrane was washed with PBS-T three times on a platform rocker and incubated with horseradish peroxidase-conjugated secondary antibody diluted in PBS-T 1:2000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Trimer was reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl, Thermo Scientific) and alkylated with 10 mM 2-chloroacetamide (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... an EdU (5-ethynyl-2′-deoxyuridine) proliferation assay was performed as recommended by Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: Fibers were incubated in 5 µM rhod-2 in the membrane permeable AM form (Invitrogen). Rhod-2 was excited with 531 nm light and the emitted light was collected through a 585 nm long-pass filter.
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bioRxiv - Molecular Biology 2022Quote: ... 5 x 107 ESCs were fixed with 2 mM DSG (disuccinimidyl glutarate, Thermo Fisher Scientific) for 50 min at 25⁰C and then 1% formaldehyde (methanol-free ...
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bioRxiv - Microbiology 2022Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope or Confocal Zeiss LSM980 - AiryScan and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Microbiology 2022Quote: Larvae were immersed in 100 μg/mL 5-ethynyl-2′-deoxyuridine (EdU) solution (A10044; Invitrogen) for 16 h before termination of the experiment ...
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bioRxiv - Cell Biology 2021Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Neuroscience 2022Quote: ... Animals were injected intraperitoneally with 40 mg/kg EdU (5-ethynyl-2’-deoxyuridine; Invitrogen, E10187) before the session ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... Slides were counterstained with 5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; ThermoFisher Scientific) for 10 min at room temperature and washed with 1 × PBST (0.2% Triton-X 100 ...
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bioRxiv - Neuroscience 2022Quote: ... Animals were injected intraperitoneally with 40 mg/kg EdU (5- ethynyl-2’-deoxyuridine; Invitrogen, E10187) before and after the session ...
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bioRxiv - Neuroscience 2022Quote: Mice were injected with 100 mg/kg of 5-ethynyl-2’-deoxyuridine (EdU; Invitrogen #E10187) by intraperitoneal injection for 5 consecutive days ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500, ThermoFisher 32-2500), Rabbit anti-Myc polyclonal (1:500 ...
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bioRxiv - Biochemistry 2024Quote: ... Acclaim PepMap 100 nanoViper C18 (Thermo Scientific, 100 μm×2 cm, 5 μm, 100 Å) was used as a trap column ...
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bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl; Thermo Scientific) and alkylated with 10 mM 2-Chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with a mix of 5 µM Fura-2 AM (Invitrogen™, F1221) and 0.025% Pluronic® F-127 (diluted in CM ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM Hepes-K+ pH 7.6) containing 2 mg/mL of collagenase type II (GIBCO) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Reduction of proteins was achieved with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Thermo Fisher) for 30 min and samples were subsequently alkylated in 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Nuclei were counterstained by Hoechst 33342 (Thermo Fisher Scientific, 2 μg/mL, 5 min, RT), and slides were mounted in ProLong Gold antifade mountant (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μM purified DNMT3A2 was incubated in triplicate with 5× SYPRO Orange (Thermo Fisher Scientific) in assay buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Recovered nuclei were stained with FITC-conujugated α-5-bromo-2’-deoxyurine (Invitrogen, MoBu-1) in staining buffer (2 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... 5-ethynyl-2’-deoxyuridine (EdU, 12.5 mg/kg, Thermo Fisher Scientific Inc. Waltham, MA, USA) was intraperitoneally injected into pregnant C57BL/6 mice once a day (12 P.M. ...
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bioRxiv - Developmental Biology 2023Quote: Tadpoles were immersed in a solution containing 1 mM EdU (5-ethynyl-2’-deoxyuridine; Invitrogen) before fixation ...
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bioRxiv - Microbiology 2023Quote: ... and 2-5 μl sample were loaded onto 10-20% SDS-tricine gels (Invitrogen, #EC66252BOX) and LPS was silver stained as described previously [47] ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µL of DNase TURBO 10X Buffer and 2 µL of DNase TURBO (Invitrogen, #AM2238) were then added and the reactions are incubated at 37 °C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... 12.5 mM Glucose and 10 μM 5’-fluoro-2’-deoxyuridine (FUDR; Thermo Fisher Scientific, 10144760), an antimitotic drug that suppresses growth of glial cells ...
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bioRxiv - Immunology 2024Quote: ... senescent CD4+ T cells were treated with BrdU (5-bromo-2′-deoxyuridine; 10µM; B23151-ThermoFisher) for 72 hours ...
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bioRxiv - Neuroscience 2024Quote: ... then an IP injection of 50 mg/kg 5-ethynyl-2’-deoxyuridine (EdU (ThermoFisher Scientific)) 24 h later and were perfused after a further six days.
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bioRxiv - Immunology 2024Quote: ... approximately 2-5×106 cells were incubated in 40 μl of fixable viability dye (Invitrogen) diluted in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitation was performed with 5 µg of anti-BRN1/2 (PA5-30124 Thermo Fisher Scientific) or rabbit IgG as a negative control ...
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bioRxiv - Molecular Biology 2024Quote: ... UHRR or HEK-293T RNAs (5 μg) were DNased with 2 U TURBO DNase (Invitrogen) and purified using an RNA Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Neuroscience 2024Quote: ... Animals were injected intraperitoneally with 40 mg/kg EdU (5-ethynyl-2’-deoxyuridine; Invitrogen, E10187) before the session ...
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bioRxiv - Neuroscience 2022Quote: ... with 16 μM BSA in the presence of CaCl2 (1.3mM) and fura-2-acetoxymethyl ester (fura 2-AM, 5 μM: Molecular Probes, Eugene, OR, USA), and they were incubated at 37 °C for 25 min ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were loaded with 5 μM acetoxymethyl-ester of Fura-2 (Fura-2/AM) and 80 μg /ml pluronic F127 (Molecular Probes, Eugene, Oregon) for at least 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... then incubated at 37C for 5 min in 2 ml M3+BPYE media containing 1 ml Turbo DNase (2 U/μL) (ThermoFisher cat. no. AM2239) per 36 ml ...
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bioRxiv - Biochemistry 2021Quote: ... The 5 mL HisTrap column was washed with 10 column volumes of wash buffer (2× GIBCO 14200-075 PBS, 5 mM Imidazole, pH 7.5) followed by 6 column volumes of elution buffer (2× GIBCO 14200-075 PBS ...
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bioRxiv - Biochemistry 2024Quote: ... 300 μm × 5 mm, 5 μm, 100 Å, separation column: C18, Acclaim PepMap, 75 μm × 500 mm, 2 μm, 100 Å, Thermo Fisher Scientific). After loading the sample on the pre-column ...
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bioRxiv - Microbiology 2020Quote: ... The assay is based on the dilution of co-mixed N-(7-nitro-benz-2-oxa-1,3-diazol-4-yl)phosphatidylethanolamine (N-NBD-PE) and N-(lissamine Rhodamine B sulfonyl)phosphatidylethanolamine (N-Rh-PE) (Molecular Probes, Eugene, OR, USA), whereby dilution due to membrane mixing results in increased N-NBD-PE fluorescence ...
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bioRxiv - Molecular Biology 2020Quote: ... The tryptic peptides were loaded at 5 μL/min onto a C18 trap column (Thermo Scientific, 100 µm ID×2 cm, 5 μm, 120 Å). Peptides were separated with PicoFrit columns (New Objective ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Systems Biology 2020Quote: ... Disulfide bonds were reduced with 5 mM Tris(2-carboxyethyl)phosphine (TCEP) Bond-breaker (Thermo Scientific) at RT for 1 hr ...
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bioRxiv - Cell Biology 2020Quote: ... protected by an Acclaim PepMap C18 column (100 μm x 2 cm, 5 μm; Thermo Scientific) before injection into a Q-Exactive mass spectrometer (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... The precolumn was a 2 cm EASYcolumn (ID 100 µm, 5 µm particles) (Thermo Fisher Scientific) while the analytical column was a 10 cm EASY-column (ID 75 µm ...
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bioRxiv - Developmental Biology 2021Quote: ... The eggs were then loaded with the calcium indicator Fura-2 AM (5 μM; Thermo Fisher) for 30 min in KSOM containing 0.02% pluronic F-127 (Thermo Fisher) ...