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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... and the lentiviral envelope vector VSVg were transfected into HEK 293T cells with TurboFect (Life Technologies). Transfected HEK 293T cells were incubated for two days before the virus-laden medium was transferred to HMECs or K562 cells ...
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bioRxiv - Immunology 2021Quote: ... packaging plasmid (pCMV-dR8.2) and envelope plasmid (pCMV-VSV-G) using Lipofectamine-LTX reagent (Thermo Fisher Scientific India Pvt ...
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bioRxiv - Biophysics 2023Quote: ... and a BG505 envelope SOSIP were expressed in FreestyleTM 293-F cells (ThermoFisher Cat No. R79007). Before transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
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bioRxiv - Cell Biology 2021Quote: ... media was collected from each well at 24 h and virus RNA was isolated using MagMAX Virus/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific) and automated extraction was carried out using KingFisher™ Flex Purification System ...
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bioRxiv - Molecular Biology 2022Quote: ... Phasi Charoen like virus and Culex Y virus) were maintained at 28 °C in Leibovitz’s L-15 medium (Invitrogen: catalogue number: 21083027) supplemented with 10% foetal bovine serum (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic RNAs of PRRSV (porcine reproductive and respiratory syndrome virus) and CSFV (classic swine fever virus) were extracted following the TRIzol method (TRIzol reagent, Invitrogen, California, USA). Extracted RNA of PRRSV was examined using VDx PRRSv qRT-PCR (NA/EU dual ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid was detected by virus-specific primer/probe according to indicated reference sequences (Table 1) utilizing TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems) and QuantStudio™ 5 (ThermoScientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA concentrations of 30 samples were normalized to approximately 15 ng µl-1 and TSV was detected and quantified from 2 µl of sample using a TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems™) following the OIE recommended protocol and triplicate standards (mean R2 = 0.93 ± 0.06 ...
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Evolutionary stasis of an RNA virus indicates arbovirus re-emergence triggered by accidental releasebioRxiv - Evolutionary Biology 2019Quote: ... and virus isolates using Trizol LS® (Invitrogen, USA) and purified using Direct-zol RNA MiniPrep (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... or Sendai virus system Cytotune 2.0 (Thermo Scientific #A16517). Resulting iPSCs were clonally picked and expanded in mTESR (Stemcell Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The virus inoculum was prepared in OPTI-MEM (Gibco), and cells were incubated in SMEM at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted virus was resuspended in PBS and TRIzol (Invitrogen) extracted ...
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bioRxiv - Microbiology 2022Quote: ... and from virus in supernatant using Trizol LS (ThermoFisher). A one-step qRT-PCR kit with SYBR green from Agilent was used ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were diluted in OptiPRO SFM (ThermoFisher Scientific) serum-free medium according to the desired MOI ...
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bioRxiv - Microbiology 2020Quote: ... The virus pellet was washed in HBSS (Thermo Fisher), and centrifuged at 100,000 × g (Beckman SW 32 Ti rotor ...
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bioRxiv - Immunology 2022Quote: ... and Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen), followed by RT-PCR with Fast SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Immobilised virus was fixed with 4% formaldehyde (Thermo Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: Virus stocks were prepared by transfecting 293FT cells (Invitrogen) seeded in 10 cm cell culture dishes with the viral constructs described above ...
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bioRxiv - Microbiology 2023Quote: ... TaqMan Fast Virus 1-step Master Mix (Applied Biosystems) and primer/probes targeting NS3 primers/probe (IDT)11 ...
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bioRxiv - Microbiology 2024Quote: ... and TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Reactions were set up according to the manufacturer’s protocols using a 10 µl reaction volume and 25 ng of total RNA template ...
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bioRxiv - Cancer Biology 2024Quote: ... virus was prepared in high-glucose DMEM (Gibco, #11965092) supplemented with 10% FBS and 1% penicillin–streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: 10,000 HEK293 FRT were seeded in 96 well plates (M33089, ThermoFisher UK) and induced with tetracycline for 24 hours ...
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bioRxiv - Genomics 2019Quote: Total RNA was prepared from harvested knockdown HEK293 cells using TRIzol (ThermoFisher). Single-stranded DNA in the sense orientation was synthesized from total RNA with random priming using the GeneChip WT Amplification kit (Affymetrix) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T and HEK293 cells were cultured in DMEM 1X (Thermo Fisher Scientific) supplemented with 0.5 % Peniciline/Streptomycin 100X Solution (Biowest ...
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bioRxiv - Cell Biology 2019Quote: HeLa and HEK293 cell samples prepared in 6-well plates (Fisher Scientific) were used for the assay ...
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bioRxiv - Genetics 2019Quote: ... Plasmids were transfected into podocytes or HEK293 cells using Lipofectamine 2000 (Invitrogen). HEK293 cells stably expressing p3xFLAG-ADCK4 or BAP were selected and maintained with 1 mg/mL G418.
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bioRxiv - Neuroscience 2021Quote: HEK293 cell line were plated in 24-well plates in DMEM (Invitrogen) supplemented with 10% fetal bovine serum ...
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bioRxiv - Systems Biology 2021Quote: ... Ptk2 and HEK293 cells were incubated in imaging medium (FluoroBrite DMEM (Gibco) supplemented with 10 % FBS (Gibco) ...
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bioRxiv - Biochemistry 2021Quote: ... plasmids were transfected into HEK293-T cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... The tetracycline inducible cell line T-REx™-HEK293 (Thermo Fisher Scientific) was cultured under the following conditions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HEK293 cells using either Lipofectamine 2000 reagent (Invitrogen) or calcium phosphate transfection.
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bioRxiv - Cell Biology 2022Quote: NIH-3T3 (ATCC: CRL-1658) and HEK293-FT (HEK, ThermoFisher Scientific R70007) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 and S16 cells were cultured in DMEM (#41965, Thermofisher Scientific, USA) complemented with 10 % of FBS (#15000-036 ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells (ATCC) were maintained in DMEM (Gibco, Cat. no. 12800-017) supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... The HEK293 derivate HEK293F cell line (Cat nr R79007, Thermo Fisher Scientific) were cultured in Freestyle 293 medium ...
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bioRxiv - Cell Biology 2022Quote: ... with a C-terminal 6x His tag was custom-synthesized by Invitrogen and transiently transfected in HEK293 T cells using polyethylenimine in OPTIMEM (Invitrogen) containing 2 mM CaCl2 ...
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bioRxiv - Genetics 2021Quote: ... The plasmids were used to generate Flp-In HEK293 (Thermofisher, cat. #R78007) stable cell lines ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 suspension cells (growing in FreeStyle™ 293 Expression Medium, Gibco #12338) were transiently transfected with the vector coding for the scFv-Fc of each antibody ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 cells (ATCC) were cultured with Dulbecco’s Modified Eagle Medium (DMEM; Gibco) with 10% Fetal Bovine Serum (Biowest ...
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bioRxiv - Genetics 2020Quote: HEK293 and human fibroblast culture medium consisted of DMEM (Gibco; 11995-065) supplemented with 10% fetal bovine serum (Gibco 16000-036) ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293S GnTI− cells were cultured in suspension in FreeStyle 293 medium (Gibco) supplemented with 2% fetal bovine serum ...
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bioRxiv - Genomics 2019Quote: ... lentiviruses were produced in HEK293 cells using ViraPower Lentiviral Expression System (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: A transgenic HEK293 containing GFP gene in the genome (ThermoFisher via MTA) was used in cell experiments ...
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bioRxiv - Molecular Biology 2019Quote: ... Huh7 and HEK293 cells were maintained in DMEM GlutaMAX (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... plasmids were transfected into HEK293-T cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Thermoscientific) supplemented with 1 mM sodium pyruvate ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 GnTI- cells were grown and maintained in FreeStyle 293 medium (Gibco) supplemented with 2% fetal bovine serum ...
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bioRxiv - Molecular Biology 2020Quote: ... Splicing analysis was carried out in HEK293 cells after lipofectamine 2000 (Invitrogen) transfection of plasmids ...