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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 in Dulbecco’s modified Eagle Medium (DMEM, Life Technologies #11965118); T-47D in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Cell Biology 2023Quote: Flp-In™ T-REx™ HEK293 cells (Invitrogen, R78007) containing a single genomic FRT site and stably expressing the Tet repressor were cultured in DMEM medium supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2024Quote: HEK293 cells were transfected using Lipofectamine 3000 (Invitrogen, Cat# L3000008) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Flp-In T-Rex HEK293 cell lines (Thermo Fisher Scientific) expressing mitochondrial-relocated golgin-BirA* fusions (from John Shin ...
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bioRxiv - Biochemistry 2024Quote: ... GKO HEK293 cells were maintained in DMEM (Gibco Cat# 11995065) containing 10 % FBS (Gibco Cat# 16000044) ...
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bioRxiv - Cell Biology 2024Quote: FlpIn CHO and HEK293 cell lines were purchased from Invitrogen and maintained in Dulbecco’s Modified Eagle’s Medium supplemented with 5% Foetal Bovine Serum (ThermoFisher Scientific (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: Flp-In T-Rex HEK293 cells (Invitrogen, Thermo Fisher Scientific) were co-transfected with plasmids pOG44 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Flp-In T-Rex HEK293 cells (Invitrogen, Thermo Fisher Scientific) were co-transfected with plasmids pOG44 (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... HEK293 GnTI- cells were cultured in Freestyle 293 medium (Invitrogen) supplemented with 2% FBS on a shaker at 37 °C in the presence of 8% CO2 to a density of 3 × 106 cells per ml ...
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bioRxiv - Neuroscience 2023Quote: ... Stable HEK293 lines were generated by lipofection (Lipofectamine 2000, ThermoFisher) of ∼80% confluent cells with 2 µg of linearized expression plasmid overnight according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μl Dynabeads Protein G (Thermo Fisher Scientific, 10004D), and 400 μl dilution buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... HA Tag antibody for SaCas9 protein (2-2.2.14, Thermofisher), rabbit anti-GFP (A11122 ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 E protein was purchased from ThermoFisher Scientific (Catalog# RP-87682) ...
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bioRxiv - Immunology 2023Quote: ... recombinant SARS-CoV-2 S protein (RP-87680, Invitrogen) or recombinant SARS-CoV-2 spike RBD protein (40592-V08H ...
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bioRxiv - Microbiology 2023Quote: ... pre-stained protein markers (SeeBlue Plus 2, ThermoFisher Scientific) were included ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: RNA extraction and RT-PCR were performed as previously described.45 Total RNA was extracted from HCoV-NL63 virus infected Caco-2 cells at a MOI of 0.05 at 48 hours post infection using TRIzol reagents (Thermo Fisher Scientific). 2.0□μg of total RNA was used to synthesize the first strand cDNA of viral RNA and host RNA using SuperScript III reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: Copies of the SARS-CoV-2 N gene (genomic) were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2023Quote: ... Anesthetized mice were inoculated intranasally with 30 μL of virus diluted in chilled 1X PBS (MHV) or administered undiluted in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... Baculoviruses were amplified twice and freshly generated P3 virus was used to infect 1.8-2 L of High Five cells (Thermo Fisher Scientific) at a density of 3-3.5 x106 cells/mL by adding 6 mL virus per 600 mL of cells ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were loaded along with the SeeBlue Plus 2 protein marker (LC5925, Life Technologies). The gel was run at 60V for 20 mins ...
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bioRxiv - Immunology 2021Quote: ... were transfected to HEK293 cells (ATCC® CRL-1573™) and each recombinant protein was purified using HisPur™ Ni-NTA column (Invitrogen, Cat# 88226). The specificity of recombinant protein was further validated by SDS-PAGE and Western blot evaluations.
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bioRxiv - Molecular Biology 2022Quote: ... Wells were coated with 1 μg Gfp for anti-GfpNB or 1 μg commercially available SARS-CoV-2 Spike-RBD-domain protein for SARS-CoV-2 nanobody-Cts1-fusion proteins (Invitrogen, Waltham Massachusetts, USA). Recombinant Gfp was produced in E ...
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bioRxiv - Microbiology 2020Quote: ... or P1 SARS-CoV-2/München- 1.1/2020/929 (Munich) virus was diluted to 500 µl in 15 ml with virus dilution medium (Opti-MEM, Gibco) supplemented with 2% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Next day cells were washed with phosphate-buffered saline (PBS) and infected with virus in Virus Production Serum Free Media (VPSFM; Gibco) supplemented with 0.25-0.5 ug/mL TPCK-Trypsin ...
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bioRxiv - Immunology 2019Quote: ... and Moloney Murine Leukemia Virus reverse transcriptase (Invitrogen). Amplification of cDNAs was performed by quantitative real-time PCR reactions on a Light Cycler instrument (LC480 ...
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bioRxiv - Microbiology 2020Quote: ... Virus was diluted in infection media (DMEM (Invitrogen) supplemented with 35% BSA (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... using Sendai virus-mediated transduction (Thermofisher Scientific, #A16517) or nucleofection (Amaxa ...
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bioRxiv - Microbiology 2020Quote: ... and 50% virus solution in RPMI media (Gibco). Mosquitoes were left to feed for 1.5 h using Hemotek membrane feeder system (Discovery Workshops ...
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bioRxiv - Immunology 2021Quote: ... virus was diluted in RPMI-1640 media (Gibco) and maintained on ice ...
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bioRxiv - Neuroscience 2022Quote: ... virus was mixed with 4% fast green (Invitrogen) dissolved in saline and injected using a glass micropipette at a rate of 100 nl/min ...
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bioRxiv - Cancer Biology 2023Quote: ... then virus pellets were suspended in HBSS (Gibco). The virus titer was determined with Lenti-X™ p24 Rapid Titration ELISA Kit (Takara) ...
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bioRxiv - Genomics 2023Quote: ... infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Genetics 2024Quote: ... using Sendai virus-mediated transduction (Thermofisher Scientific, #A16517) or nucleofection (Amaxa ...
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bioRxiv - Neuroscience 2023Quote: ... Virus particles were resuspended in Neurobasal A (Invitrogen) and stored at -80 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were eluted in 2% SDS and protein concentration was measured using MicroBCA Protein Assay Kit (Thermo Fisher Scientific). Peptide isolations were digested by trypsin and SDS was removed employing Detergent Removal Spin Columns (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... Human embryonic kidney 293 cells (HEK293) were maintained in DMEM (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were then stimulated with 25 ng/ml EGF (Gibco) for 5 min (other cell lines were not stimulated) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) supplemented with 10% (v/v ...
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... HEK293-F cells were cultured in FreeStyle 293 Expression Medium (ThermoFisher). A days before transfection ...
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bioRxiv - Cell Biology 2019Quote: ... Transient transfection of HEK293 cells was carried out using Lipofectamine2000 (ThermoFisher) after cells reach a confluency of 70%.
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) were grown in DMEM/F12 media (GIBCO) supplemented with HyClone cosmic calf serum (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were cultured with Dulbecco’s Modified Eagles Media (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: HEK293 and COS-7 cells were transfected with Lipofectamine 2000 (Invitrogen) according to manufacturer’s specifications ...
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bioRxiv - Biochemistry 2020Quote: HEK293T (ATCC) and HEK293 (ATCC) cells were cultured in DMEM (Invitrogen) plus 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293 were performed with 4 μL Lipofectamine 2000 (Invitrogen) and 1-2.5 μg plasmid DNA per 35 mm2 ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were maintained in Eagle’s Minimum Essential Medium (EMEM; Gibco) and SH-SY5Y cells in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12 ...
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bioRxiv - Immunology 2021Quote: ... HEK293-hCD4 were detached using enzyme-free cell dissociation buffer (Gibco) and resuspended in FACS buffer (PBS containing 5% FBS) ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 (HEK) cells were grown in DMEM Media (Thermo Fisher Scientific) supplemented with 1mM Glutamax (Thermo Fisher Scientific) ...