Labshake search
Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Contactin Associated Protein Like 3 CNTNAP3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: Murine macrophage-like RAW 264.7 cells and human A549 lung epithelial carcinoma cells were cultivated in RPMI 1640 medium (Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... were chosen for this study due to the absence of endogenous ASIC-like currents [53] and were grown using standard procedures in the following medium: Ham’s F-12 Nutrient Mixture (Life Technologies), 10 % fetal bovine serum (Sigma) ...
-
bioRxiv - Biochemistry 2019Quote: ... ATCC CCL-2) cells and 3T3-L1 (murine embryo fibroblast-like cells, ATCC CL-173) were grown in DMEM (Gibco) supplemented with 10% FBS (Sigma) ...
-
bioRxiv - Plant Biology 2022Quote: ... Like samples were combined into a single tube then quantified by high sensitivity DNA Qubit (ThermoFisher scientific, Cat. No. Q32854).
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... For both fungi, first we obtained fresh blastospores (i.e., yeast-like single cells) suspended in Grace’s Insect Medium (Gibco, Thermo Fisher Scientific) supplemented with 2.5% FBS (Gibco ...
-
bioRxiv - Immunology 2023Quote: Human embryonic kidney (HEK) epithelial-like cell line HEK293T was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (11965-118; GIBCO™, Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2023Quote: ... resuspended in extracellular-like Ca2+-free buffer (120mM NaCl; 5mM KCl; 1mM KH2PO4; 0.2mM MgCl2·6H2O (Fisher Scientific #M35-500); 0.1mM EGTA ...
-
bioRxiv - Physiology 2023Quote: Total RNA was extracted from cryo-pulverized liver tissue and hiPSC-derived hepatocyte-like cells using Trizol (Thermo Fisher Scientific) and 1 ug RNA was reverse-transcribed using SuperScriptTM IV VILOTM Master Mix (#11756050 ...
-
bioRxiv - Neuroscience 2020Quote: ... after 3 washes with PBS cells were incubated with secondary antibody (Alexa Fluor conjugated antibody, 1:500, Molecular probes, Invitrogen) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... after 3 washes with PBS cells were incubated with secondary antibody (Alexa Fluor conjugated antibody, 1:500, Molecular probes, Invitrogen) for 1 hour at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... tissue sections were washed 3 times with PBS-T and the following secondary antibodies were used to detect the primary antibodies: Streptavidin-AF555 (Invitrogen), anti-rat IgG2a-AF488 (SouthernBiotech) ...
-
bioRxiv - Genomics 2023Quote: ... A western blot was performed on 3 of the monoclonal lines to confirm knockout (primary antibody used: MLH1 Monoclonal Antibody; Invitrogen, Cat No ...
-
bioRxiv - Cell Biology 2023Quote: ... and incubated with primary antibodies (Supplemental Table 3) followed by secondary antibodies (goat anti-rabbit IgG Alexa 488, 1:200, Invitrogen; goat anti-mouse IgG Alexa Fluor 594 ...
-
bioRxiv - Immunology 2023Quote: ... D1-3 and D4-5 proteins were fluorescently labelled with Alexa Fluor™ 594 or 488 Microscale Protein Labeling Kits (Invitrogen™, A30008 or A30006) as described [15] ...
-
bioRxiv - Developmental Biology 2020Quote: ... The biliary tree fragments and associated stroma were then dissociated into single cells with TrypLE 5x (Gibco, A12177-01), incubated with fluorophore-conjugated antibodies for 30min (see Supplementary Table 1 ...
-
bioRxiv - Microbiology 2020Quote: ... Measurements and analysis were performed using an ABI7500 instrument and associated software package (Applied Biosystems, Foster City, CA, USA).
-
bioRxiv - Microbiology 2023Quote: ... The gDNA was quantified using Qubit 2.0 Fluorometer and associated Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, USA). The quality was assessed by horizonal agarose gel electrophoresis and ImplenNanoPhotometer N60 (Implen ...
-
bioRxiv - Developmental Biology 2023Quote: ... a 10-min incubation at RT with streptavidin associated with peroxidase (UltraVision Detection System HRP kit, Thermo Fisher Scientific) was performed ...
-
bioRxiv - Developmental Biology 2023Quote: ... Rho associated kinase inhibitor (10μM, Y-27632) and Geltrex (0.5 μl/cm2 well surface area, Thermo Fisher Scientific, A1413302) were added to media during replating ...
-
bioRxiv - Genetics 2024Quote: ... The remaining pellet was collected as the chromatin-associated fraction.Total RNA from the cytoplasmic and nuclear fractions was extracted by using PureLink RNA MiniKit (Invitrogen) following the manufacturer’s instructions for the RNA extraction from aqueous solutions ...
-
bioRxiv - Cancer Biology 2024Quote: ... human mammary fibroblast (MF) and cancer associated fibroblast (CAF) were maintained in DMEM/F-12 (Thermo Fisher Scientific, #21331046) supplemented with 2 mM Glutamine (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... the antibody-protein-chromatin complex was retrieved by adding 25μl pre-washed Protein G Dynabeads (Thermo Fisher Scientific, 10004D). Immunoprecipitated chromatin DNA was de-crosslinked by 65°C heating overnight using elution buffer (1% SDS ...
-
bioRxiv - Genomics 2020Quote: ... Pooled fractions were tumbled at 4°C for 1 hour with 5μg of antibody (Key Resources Table) and 25μL Dynabeads™ Protein A or Protein G (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... Antibody proteins were purified from the culture supernatant after 12-14 days using Protein A bead columns (Thermo Fisher).
-
bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
-
bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
-
bioRxiv - Molecular Biology 2020Quote: ... CSDE1 protein was then captured by incubation with an affinity purified CSDE1 antibody conjugated to protein A Dynabeads (Invitrogen) for 4 hours at 4°C with gentle rotation ...
-
bioRxiv - Molecular Biology 2023Quote: ... Precipitation of the protein-antibody conjugate was performed incubating with Dynabeads® Protein A (Thermo Scientific, Carlsbad, CA, USA) 40 minutes at 4ºC in a rotary shaker ...
-
bioRxiv - Molecular Biology 2022Quote: ... The RNA-protein-antibody complex was captured using protein A/G magnetic beads (Thermo Fisher Scientific, 10002D and 10004D). Magnetic beads were washed with low salt buffer (1X PBS ...
-
bioRxiv - Genomics 2024Quote: ... 5-10 μg of antibody was coupled to 50-100 μl of Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody ZG003 (Thermo Fisher Scientific, Waltham, Massachusetts, US) were incubated at 4 °C overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... The antibodies (3 μg/IP) used include anti-GFP mouse monoclonal 3E6 (Thermo Fisher), Anti-GFP rabbit polyclonal A-11120 (Thermo Fisher ...
-
bioRxiv - Neuroscience 2019Quote: ... probed overnight with the following primary antibodies diluted in 3% BSA (Fisher Scientific BP1600): RAI1 (1:1000) ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were fixed and allowed to bind with TLR 3 antibody (Invitrogen-PA5-29619) and Alexa 594 conjugated secondary anti-rabbit goat antibody (Invitrogen-A11012) ...
-
bioRxiv - Neuroscience 2022Quote: ... Secondary antibody (2-3% serum, 0.4% PBST, 1:500 Invitrogen A11035, 1:500 DAPI) was applied and incubated for one hour at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... rabbit anti-claudin 3 polyclonal antibody (1:100 dilution, PA5-16867, Thermo Fisher Scientific); mouse anti-chondroitin sulfate antibody (1:100 dilution ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein samples were separated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) (3-8% gradient gels, Invitrogen) and subsequently transferred onto nitrocellulose membranes ...
-
bioRxiv - Molecular Biology 2021Quote: ... The protein was dialyzed in a Slide-a-Lyzer cassette with a 3 kDa cutoff (Thermo Scientific) in step-wise gradient of NaCl and imidazole before final dialysis for 16-18h in 50 mM Tris-HCl ...
-
bioRxiv - Microbiology 2021Quote: ... Protein levels of IL-18 from intestinal biopsies were measured by ELISA (BMS618-3, ThermoFisher, Waltham, MA). Assays were performed according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2021Quote: ... Processed proteins samples were run on 3–8% NuPAGE Tris-Acetate 1.5mM pre-cast gels (ThermoFisher, EA0378BOX) and primary antibodies used were ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein lysates were loaded into 3-8% Tris-Acetate mini gels (Thermo Fisher Scientific, cat no. EA03785BOX), separated electrophoretically ...
-
bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Immunoprecipitation was performed at 4°C for 3 hr with 50 µl protein G dynabeads (Thermo Fisher). Beads were washed five times with ice-cold Wash buffer 1 (50 mM Hepes-KOH (pH7.6) ...
-
bioRxiv - Systems Biology 2019Quote: ... 3-30μg protein per well was separated using NuPAGE precast 4-12% polyacrylamide gel system (ThermoFisher Scientific). Immunoblotting was carried out using an X-Cell II blot module (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... Equal protein lysates were run on Novex 3– 8% Tris-acetate 15 Well Mini Gels (EA03785BOX, Invitrogen) and transferred to Immobilon-P membranes (IPVH00010 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Equal protein lysates were run on Novex 3–8% Tris-acetate 15 Well Mini Gels (EA03785BOX, Invitrogen) and transferred to Immobilon-P membranes (IPVH00010 ...
-
bioRxiv - Biochemistry 2022Quote: ... following the separation of protein complexes on 3–12% precast Bis-Tris Native PAGE gels (Life Technologies), the proteins were transferred to polyvinylidene difluoride (PVDF ...
-
bioRxiv - Biochemistry 2022Quote: ... Samples were run through precast Native PAGE Novex 3-12 % Bis-Tris Protein Gels (Thermo Fisher Scientific) according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were blotted onto PVDF membranes using iBlot (program 3 for 8 min; Invitrogen, Carlsbad, CA, USA). Blots were blocked in 5% nonfat dry milk (NFDM ...
-
bioRxiv - Plant Biology 2023Quote: ... Proteins were separated by Native PAGE™ Novex® 3–12% Bis-Tris Gels (BN1001, Invitrogen™) and transferred to PVDF membrane using an eBlot™ L1 transfer system (GenScript) ...