Labshake search
Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2024Quote: ... Human GPR108 (NCBI RefSeq NP_001073921) was synthesized as GeneArt Strings by Invitrogen and cloned into pSBbi using Gibson cloning.
-
bioRxiv - Developmental Biology 2024Quote: ... human or murine aortic SMCs were transfected with Lipofectamine 2000 (Life Technologies) containing Scr RNA or siRNA targeting ELN ...
-
bioRxiv - Cell Biology 2020Quote: The breast cancer cell line MDA-MB-468 was cultured at 37°C and 5% CO2 in RPMI medium supplemented with 10% (v/v) fetal calf serum (FCS) and Antibiotic-Antimycotic (Thermo Fisher Scientific, Schwerte, Germany). The keratinocyte cell line HaCaT ...
-
bioRxiv - Cell Biology 2019Quote: ... Live-cell imaging experiments were performed using 8-well ibidi chambers (Vitaris, Switzerland) and imaging medium (Leibovitz L-15 medium from Thermofisher, supplemented with 10 % FCS). The following drug concentrations were used ...
-
bioRxiv - Cancer Biology 2022Quote: ... Japan) and was measured at an optical density (OD) of 450 nm with a Multiskan FC microplate photometer (Thermo Fisher Scientific, Waltham, MA, USA). For the cell proliferation assay ...
-
bioRxiv - Immunology 2020Quote: Analysis of IgG Fc-glycosylation was performed with nanoLC reverse phase (RP)-electrospray (ESI)-MS on an Ultimate 3000 RSLCnano system (Dionex/Thermo Scientific, Breda, The Netherlands) coupled to an amaZon speed ion trap MS (Bruker Daltonics ...
-
bioRxiv - Immunology 2020Quote: ... Briefly, TILs were cultured in RPMI 1640 culture medium (SERO-Med GmbH, Wien) supplemented with 10% FCS HyClone (Gibco Laboratories, Grand Island, NY, USA) and stimulated for 5 h using the Leukocyte Activation Cocktail ...
-
bioRxiv - Biochemistry 2022Quote: ... Prostate cancer cell lines were cultured in RPMI medium supplemented with 2 mM L-glutamine and 10% heat-inactivated FCS (ThermoFisher Scientific, Melbourne, VIC, Australia). Cell media was changed every three days and cells were passaged regularly at ∼80-90% confluency by trypsinization ...
-
bioRxiv - Cell Biology 2022Quote: ... the confocal volume was determined by performing FCS using a dye with known diffusion coefficient and concentration (Alexa Fluor 488 NHS ester; Thermo Fisher Scientific for mEGFP). To convert fluorescence intensity to the concentration ...
-
bioRxiv - Immunology 2019Quote: Analysis of IgG Fc-glycosylation was performed with nanoLC reverse phase (RP)-electrospray (ESI)-MS on an Ultimate 3000 RSLCnano system (Dionex/Thermo Scientific, Breda, The Netherlands) coupled to a Maxis HD quadrupole-time-of-flight MS equipped with a nanoBooster (Bruker Daltonics ...
-
bioRxiv - Microbiology 2022Quote: ... Staining for surface markers was performed by incubating the cells in single cell suspension with anti-mouse CD16/CD32 mouse Fc block (Thermo Fisher Scientific, RRID: AB_467135) and 10% rat-IgG in PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... Media was removed and cells were subsequently incubated in 100µg/ml pHrhodo Green Dextran-containing complete DMEM media (10% FCS (Fisher Scientific, Cat#: 10-438-026), 1% P/S (Gibco ...
-
bioRxiv - Microbiology 2023Quote: The growth burden imposed by lateral flagella was determined in an automated growth experiment in a MultiskanTM FC microplate photometer (Thermo Scientific, software SkanIt 4.1). The wells of a 96-well microplate were filled with 200 μl of either MB alone (blank wells and border wells ...
-
bioRxiv - Immunology 2023Quote: ... The fragment antigen-binding (Fab) part was separated from undigested IgG and the crystallizable fragment (Fc) using an Immobilized Protein A column (Thermo Fisher Scientific, No. 20356). The flow through containing the Fab was concentrated through 10 kDa Amicon centrifugal filter units (EMD Millipore ...
-
Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... to a microplate for the absorbance measurement at 765 nm with a Varioskan Flash plate reader (Multiskan FC Microplate Photometer, Thermo Scientific, Waltham, MA, USA). A calibration curve of the standard compound gallic acid with a linearity range from 50 mg/L to 800 mg/L was constructed ...
-
bioRxiv - Immunology 2023Quote: Mouse peripheral blood was processed to remove RBC via lysis prior to Fc blocking and then stained with antibodies against surface markers including biotin-CD93 (Invitrogen, cat # 13-5892-85), BV605-CD19 (BD ...
-
bioRxiv - Immunology 2023Quote: ... the cultured B cells were stained with Fc block followed by fixable viability dye eFluor 780 and FITC BAFF-R monoclonal antibody (Invitrogen; cat #11-5943-81) and analyzed on a LSRFortessa X-20 flow cytometer (BD).
-
bioRxiv - Cancer Biology 2021Quote: Human melanocytes and melanoma cells were lysed in Pierce RIPA buffer (Thermo Scientific) containing 1× Halt protease inhibitor cocktail (100× ...
-
bioRxiv - Cancer Biology 2021Quote: ... Human CRC organoids medium is composed of Ad-DF+++ (Advanced DMEM/F12 (GIBCO) supplemented with 2 mM Ultraglutamine I (Lonza) ...
-
bioRxiv - Cell Biology 2020Quote: Primary human umbilical vein endothelial cells (HUVECs) were obtained from Gibco (Cat#: C0035C) and cultured in Medium 200 (Cat# ...
-
bioRxiv - Cell Biology 2020Quote: ... HIF1A levels were measured in duplicates applying a HIF1A Human ELISA kit (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: Human induced pluripotent stem cells (hiPSCs) were cultured in StemFlex media (Gibco, #A3349401) and passaged with EDTA (Life Technologies #15575-020) ...
-
bioRxiv - Immunology 2021Quote: ... HRP conjugated Rabbit anti-Human IgG(H+L) (Cat. No. A18903, Invitrogen, USA) as added to each well as the detection antibody ...
-
bioRxiv - Genetics 2019Quote: ... RPE1 cells and primary human fibroblasts were cultivated in complete DMEM medium (Gibco).
-
bioRxiv - Molecular Biology 2021Quote: Human MSCs were maintained in growth media (Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing D-glucose (1g/L) ...
-
bioRxiv - Molecular Biology 2021Quote: ... supplemented with human keratinocyte growth supplement (catalogue #S-001-5, Thermo Fisher Scientific) and 1% penicillin/streptomycin (catalogue #15140-122 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed using the Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermofisher). Templating and sequencing were performed with the Ion PI HiQ OT200 kit ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
-
bioRxiv - Immunology 2021Quote: For polyreactivity ELISAs against human insulin (MilliporeSigma) and dsDNA (Calf Thymus DNA; Invitrogen), plates were coated with 2µg/ml and 50µg/ml ...
-
bioRxiv - Immunology 2021Quote: After 48 h of stimulation with Human T-activator CD3/CD28 Dynabeads (ThermoFisher) CD4+ lymphoblasts were recovered for transfection of CRISPR/Cas9 nucleoprotein complexes ...
-
Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... and 10 µl/ml Fibronectin from human plasma (Thermo Fisher Scientific, #33016-015)] for 2-3 h at 37 °C/5% CO2 and stored at 4 °C after thorough removal of coating solution.
-
bioRxiv - Physiology 2019Quote: Human embryonic kidney (HEK-293) cells were cultured in DMEM (Gibco, Basel, Switzerland) supplemented with 10% FBS ...
-
bioRxiv - Genomics 2019Quote: We used GeneChip Human Exon 1.0 ST arrays (Affymetrix, Santa Clara, CA, USA) to measure the expression levels of transcripts in each brain tissue specimen ...
-
bioRxiv - Cell Biology 2019Quote: ... Human M-CSF and Ficoll histopaque were purchased from Invitrogen (Carlsbad, CA, USA). Human CD14 magnetic beads were obtained from Miltenyi Biotec (Auburn ...
-
bioRxiv - Cancer Biology 2019Quote: ... Mouse and human PSCs were cultured in DMEM (10-013-CV; Fisher Scientific) containing 5% FBS ...
-
bioRxiv - Immunology 2019Quote: ... and sCD163 (Thermo Scientific™ Pierce™ Human CD163 Kit, Thermo Scientific, USA) in plasma.
-
bioRxiv - Immunology 2020Quote: ... Human embryonic kidney (HEK293T) cells and HeLa cells were cultured in DMEM (Gibco) supplemented with 100U/ml Penicillin (Sigma-Aldrich),100μg/ml Streptomycin (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2019Quote: ... T Cells were stimulated with Dynabeads™ Human T-Expander CD3/CD28 (Gibco). The cells were cultured in GRex (Wilson Wolfe ...
-
bioRxiv - Cancer Biology 2019Quote: ... Amyloid Beta-40 ELISA was performed using the Human AB40 ELISA Kit (Invitrogen). Secretion values were normalized to protein content of wells as measured by RIPA harvest and BCA protein quantification.
-
bioRxiv - Immunology 2020Quote: ... T cells were stimulated with Dynabeads® Human T expander CD3/CD28 (Invitrogen) at a 1:3 ratio (T cell:bead) ...
-
bioRxiv - Genomics 2019Quote: Human colon carcinoma cells HT-29 were cultured in 1640 medium (Gibco, USA) supplemented with 10% (v/v ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Cells stained with goat anti-human IgG Alexa Fluor 647 (Life Technologies, USA) and Donkey anti-human IgM Alexa FITC (Southern Biotech ...
-
bioRxiv - Immunology 2019Quote: ... anti-human CD8 conjugated to Pacific Blue (clone 3B5, cat. no. MHCD0828, Invitrogen), anti-human CD39 conjugated to APC (clone A1 ...
-
bioRxiv - Microbiology 2019Quote: ... 5% heat-inactivated AB human serum and 20 ng/mL M-CSF (Invitrogen). Cells were washed with PBS and resuspended in fresh media on days 3 and 6 of differentiation ...
-
bioRxiv - Cancer Biology 2019Quote: Human RPE1-hTERT cell lines were grown in DMEM+GlutaMAX-I (Gibco, 10569) supplemented with 10% FBS and 1% Pen Strep (100 U/ml) ...
-
bioRxiv - Genetics 2021Quote: Wild-type human FAN1 gene was cloned in a derivative of pFASTBAC1 (Invitrogen) with GST- ...
-
bioRxiv - Microbiology 2021Quote: ... 10 ng/ml of recombinant human fibroblast growth factor 2 (FGF2, Life Technologies), 20 ng/ml of recombinant human epidermal growth factor (EGF ...
-
bioRxiv - Genomics 2020Quote: ... 5% A+ human serum (Interstate Blood-Bank) and 0.5% AlbuMAX II (Life Technologies), 5% HC ...
-
bioRxiv - Cell Biology 2021Quote: ... human bone marrow aspirates were diluted in phosphate buffered saline (PBS, Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... The sensor was then pulsed with increasing concentrations of purified human APC (ThermoFisher) in 20 mM Hepes pH 7.4 ...