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Citations for Thermo Fisher :
4601 - 4650 of 10000+ citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Primary human T cells were cultured in AIM-V medium (Invitrogen 12055-083) supplemented with 5% Human serum (Sigma H4522-100ML) ...
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bioRxiv - Cancer Biology 2024Quote: ... A549 human lung adenocarcinoma cells (CCL_185, ATCC) were cultured in DMEM (Gibco, #41965039), and supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: Human embryonic kidney (HEK)293T (ATCC CRL-11268) and FreeStyle 293F (Life Technologies) cell lines were used for recombinant protein productions ...
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bioRxiv - Immunology 2024Quote: Human serum derived macrophages were lifted using TrypLE (5 mL, Thermo Fisher Scientific). Once lifted ...
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bioRxiv - Cell Biology 2024Quote: ... and a human proteome database (UniProt) was screened with SEQUEST (Thermo Fisher Scientific). The normalization node was added to the processing workflow ...
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bioRxiv - Immunology 2024Quote: Human and murine B cells were lysed in RIPA buffer (Thermo Fisher, #89900) supplemented with protease inhibitors (Roche ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were activated with DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher, 11131D) at a 1:1 cell-to-bead ratio and cultured in 24-well plates with 1 million cells per mL at 37◦C and 5% CO2 overnight.
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bioRxiv - Immunology 2024Quote: ... in combination (each at 100ng/mL) or with Dynabeads human T-Activator (ThermoFisher) anti-CD3/CD28 beads (at a 1:1 bead/cell ratio) ...
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bioRxiv - Molecular Biology 2024Quote: ... the CECs were resuspended in stabilisation media (containing Human Endothelial-SFM (Life technologies) supplemented with 5% foetal bovine serum (FBS) ...
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bioRxiv - Immunology 2024Quote: ... ELISA plates were coated with anti-human IgG (ThermoFisher 88-50550-88, 1X) and blocked overnight with 2X assay buffer (Invitrogen 88-50550-88 ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed twice with cold FACS buffer (PBS + 2% FCS) and incubated in the dark at 4° C with an anti-TfR1 (CD71) antibody (clone OKT9; Thermo Fisher Scientific, Waltham, MA, USA). Cells were then washed with PBS and resuspended in FACS buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... The suspended material was filtered through a 76 μm sterile filter and subsequently cultured in DMEM/F12 phenol red-free medium supplemented with 10% heat-inactivated fetal calf serum (FCS; Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin/streptomycin (C-medium) ...
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bioRxiv - Developmental Biology 2020Quote: 30,000 thawed hESCs were cultured in single wells of a 48-wells plate and decidualized by adding DMEM/F12 phenol red-free medium supplemented with 10% heat-inactivated charcoal stripped FCS (Thermo Fisher Scientific, Waltham, MA, USA), 0.5 mM 8-bromoadenosine 3□,5□-cyclic monophosphate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: ... All media were supplemented with 10% Foetal Calf Serum (FCS) (First Link, Birmingham, UK) as well as 100 units/mL penicillin and 100 μg/mL streptomycin (Gibco™ through Thermo Fisher Scientific, Loughborough, UK). All cell types were cultured at 5% carbon dioxide (CO2 ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: 3T3-F442A preadipocytes were grown and differentiated in a modified DMEM designed for isotope labeling in cell culture [Sigma Aldrich (Saint Louis, MO, USA)] supplemented with 10% dialyzed FCS (GIBCO-Thermo Fisher Scientific, Eugene, OR, USA), 125mg/mL streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... with formulations calling for L-WRN conditioned medium instead using 500 pM Gibco™ Wnt Surrogate-Fc Fusion Recombinant Protein (Thermo Fisher Scientific, cat# PHG0402), 500 ng/mL recombinant RSPO1 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The change in absorbance at 340 nm due to oxidation of NADH was recorded in a Multiskan FC Microplate Photometer (Thermo Fisher Scientific, Waltham, MA, USA). The data were fitted to the Michaelis-Menten equation to obtain values for the actin concentration at half-maximal activation of ATP-turnover (Kapp ...
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bioRxiv - Cancer Biology 2022Quote: The detection of TGF-β in the cell culture media from SH-SY5Y and MDA-MB-231 cells treated with TEPA for 24h was performed according to the protocol suggested by Thermo Fisher (TGF-beta-1-Human-ELISA-Kit/BMS249-4).
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bioRxiv - Synthetic Biology 2021Quote: ... CD3+ T cells were activated with anti-human CD3/CD28 magnetic Dynabeads (CTS, ThermoFisher) in a 1:1 ratio with 100 U mL-1 of IL-7 and 10U mL-1 IL-15 (R&D Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... FAM-labeled target primer and endogenous human ACTB control (beta actin) (Thermo Fisher Scientific) were mixed with master mix and template ...
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bioRxiv - Microbiology 2020Quote: ... Human embryonic stem cell (hESC; H9)-derived neural stem cells (NSC) (Thermo Fisher Scientific) were cultured ...
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bioRxiv - Microbiology 2020Quote: A549 human epithelial cells (ATCC ref# CCL-185) were maintained in F12K media (Gibco) supplemented with 1x GlutaMax (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... Taqman probes targeting human GAPDH (Hs02758991_g1) and IFIT1 (Hs03027069_s1) were purchased from Life Technologies. qRT-PCR data were collected on a StepOnePlus™ Thermal Cycler (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2021Quote: Membranes were coated with an anti-human IgG capture antibody (Thermo Fisher Scientific, A18813). Cells were then added to the plate in duplicate in 2 fold serial dilutions and incubated for 24hrs prior to detection using an alkaline phosphatase-linked anti-human IgG detection antibody (Jackson ...
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bioRxiv - Genomics 2021Quote: ... Human K562 erythroleukemia cells were cultured in Roswell Park Memorial Institute (RPMI, Life Technologies) 1640 medium supplemented with 10% FBS ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Total RNA was isolated from human desmoid tumor cell lines using Trizol reagent (Invitrogen) according to the manufacturer instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Primed human ESC and iPSC cells were harvested with 0.25% trypsin-EDTA solution (Invitrogen), and cells resuspended in PBS were transfected with 75μg DNA constructs (Gene Pulser Xcell System ...
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bioRxiv - Microbiology 2022Quote: ... human foreskin fibroblast (HFF) monolayers were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplemented with 25 mM glucose (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 20 ng/mL human recombinant basic fibroblast growth factor (bFGF; Invitrogen, USA), 20 ng/mL epidermal growth factor (EGF ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated with Alexa Fluor 488 goat anti-human IgG(H+L) (Invitrogen) at 1:500 (in 3% BSA/PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... except for human XBP1S that was assessed using SYBR green master mix (Thermofisher Scientific) using primers in Supplementary Table 3.
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bioRxiv - Molecular Biology 2021Quote: ... Stable integration of CLIP-UPF1SL into human Flp-InTM T-RExTM-293 cells (Invitrogen) and subsequent maintenance of this stable line was previously described (Kishor et al ...
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bioRxiv - Genomics 2020Quote: The THP1 (human acute monocytic leukemia derived; ATCC number TIB-202 purchased from Thermofisher) cell line[18] was regularly maintained in RPMI 1640 medium containing GlutaMAX™ ...
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bioRxiv - Genomics 2020Quote: ... Samples passing quality control were analyzed using the human Clariom D Assay (ThermoFisher Scientific) and analyzed using Transcriptome Analysis Console (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... and stained with Alexa 647-conjugated anti-human IgG (H+L) antibody (Thermo Fisher) for 1h at 4°C ...
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bioRxiv - Pathology 2020Quote: ... SMCs were transfected with control siRNA or siRNA targeting the human calpain-2 (Ambion - Silencer Select validated siRNA ...
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bioRxiv - Biophysics 2019Quote: Homo sapiens SUR1 and human Kir6.2 were subcloned into pcDNA4/TO (Invitrogen; Carlsbad, CA) for expression in HEK-293T cells ...
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bioRxiv - Genomics 2019Quote: ... dataset (which used an Affymetrix platform, [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array); the default importer in BRB-ArrayTools was used to collate the data from .cel files ...
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bioRxiv - Immunology 2019Quote: ... anti-human CD3 conjugated to FITC (clone OKT3, cat. no. 11-0037-41, Invitrogen), anti-human CD8 conjugated to Pacific Blue (clone 3B5 ...
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bioRxiv - Immunology 2019Quote: ... Cortactin expression was assessed using rabbit anti-human cortactin antibody (PA5-27134; Life Technologies) stained in conjugation with goat anti-rabbit Cy3 (Life Technologies) ...
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bioRxiv - Genetics 2019Quote: ... cells were incubated in differentiation medium (DM) (10 µg/ml recombinant human insulin (Gibco), 2% HI horse serum (Thermo Fisher) ...
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bioRxiv - Genomics 2019Quote: ... We used the Genome-Wide Human SNP Array 6.0 (Affymetrix, Santa Clara, CA, USA) for genotyping single nucleotide polymorphisms (SNPs) ...
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bioRxiv - Immunology 2021Quote: ... coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher, #H17000) in 0.1 M carbonate buffer ...
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bioRxiv - Immunology 2021Quote: ... Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific) and cultured in DMEM medium (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: Human GCDH cDNA (clone ID 3142978) in pOTB7 vector was obtained from Thermo Fisher Scientific Open Biosystems repository (now part of Horizon Discovery) ...
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bioRxiv - Biochemistry 2020Quote: ... 7.5 μg/mL human IgG and 50 μg/mL BSA-biotin (Thermo Fisher Scientific) were also printed to serve as a printing control and “intra-well signal normalizer” ...
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bioRxiv - Biochemistry 2020Quote: ... and negative selection using the Dynabeads Untouched Human CD4 T Cells kit (Invitrogen 11346D) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: Human embryonic kidney (HEK)293T cells (ATCC, CRL-11268) were grown in DMEM (Gibco) plus 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Biophysics 2021Quote: ... CD4+ T cells were stimulated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) and cultured in AIM V Medium (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS (Gibco ...