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Citations for Thermo Fisher :
4301 - 4350 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Goat anti-human IgG (H + L) (Thermo Fisher Scientific) or Goat anti-Human IgG Fc recombinant (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... 2 mM L-glutamine (Thermo Fisher Scientific, #25030-024), Insulin-Transferrin-Selenium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM L-Glutamine (Gibco (Thermo Fisher Scientific Inc.)) ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM L-Glutamine (Gibco (Thermo Fisher Scientific Inc.)) ...
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bioRxiv - Microbiology 2024Quote: ... Goat anti-human IgG (H+L) (Thermo Fisher Scientific) pre-coupled to Alexa Fluor 647 were used as secondary antibodies in flow cytometry experiments ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-mouse IgG (H+L) AlexaFluor 488 (ThermoFisher) and goat anti-guinea-pig IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-mouse IgG (H+L) AlexaFluor 488 (ThermoFisher) and goat anti-chicken IgY (H+L ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10 % FBS (Biowest) and L-glutamine (Gibco). After 24 h at 37 °C ...
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bioRxiv - Physiology 2024Quote: ... and donkey anti-rabbit IgG (H + L) (Invitrogen A32802)] were incubated with the membrane at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... containing either light Arg/Lys or heavy isotope-labeled Arg (13C6 15N4 L-arginine)/Lys (13C6 15N2 L-lysine) (Thermo Fisher Scientific, respectively) at least six doubling times ...
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bioRxiv - Biochemistry 2021Quote: Fibroblasts were cultured in DMEM with 4,5 g/L glucose, L-glutamine and sodium pyruvate (PAN-Biotech, Aidenbach, Germany) supplemented with 10% fetal bovine serum (FBS) (ThermoFisher Scientific, Waltham, MA) and 1% Penicillin/Streptomycin (PAN-Biotech ...
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bioRxiv - Neuroscience 2021Quote: ... before cells were treated for 3 hours with nutrients (1 µM insulin plus 0.8 mM L-leucine [Sigma Aldrich catalog numbers I5500 and L8000, respectively] and 0.4mM L-arginine [Acros Organics catalog number 105001000)]) ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor 488 Donkey anti-Rabbit IgG (H+L, Catalog # A-21206), Alexa Fluor 647 Donkey anti-Rabbit IgG (H+L, Catalog # A-31573) were purchased from Invitrogen (Manufacturer: Life Technologies). For the WT mice used for restraint stress ...
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bioRxiv - Biochemistry 2019Quote: ... The mixture was then alkylated using 3 µL of 400 mmol/L 2-chloroacetamide (CAA, Thermo Scientific; final concentration of 40 mmol/L) and incubated in the dark at room temperature for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Alexa Fluor 555 goat anti-mouse IgG (H+L) and Alexa Fluor 488 goat anti-rabbit IgG (H+L) were purchased from Life Technologies (MA, USA). Secondary antibodies for Western blot studies were from LI-COR Biosciences (Bad-Homburg ...
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bioRxiv - Cell Biology 2024Quote: ... Goat anti-Rabbit IgG (H+L)-Alexa Fluor™ 488 (#A11070) and Goat anti-Mouse IgG (H+L)-Alexa Fluor™ 568 (#A11019) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... unconjugated primary antibodies were detected with fluorescently-labelled species appropriate secondary antibodies including Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 and Goat Anti-Rat IgG (H+L) Alexa Fluor 647 (ThermoFisher Scientific, Brisbane, Australia). All IF sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2023Quote: ... and then with Alexa Fluor 594-conjugated donkey anti-goat IgG (H+L) and Alexa Fluor 488-conjugated donkey anti-mouse IgG (H+L) antibodies (Invitrogen; cat # A-21202). The stained sections were preserved in Vectashield antifade mounting medium with DAPI (Vector Laboratories ...
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bioRxiv - Genetics 2024Quote: ... and these primary antibodies were detected by the secondary antibodies Goat anti-Rabbit IgG (H+L) - Alexa Fluor 555 and Goat anti-Mouse IgG (H+L) - Alexa Fluor 488 (Invitrogen, A11001, 1:4000), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured on L-Laminin and Poly-L ornithine pre-coated flasks/plates in the presence of Neurobasal Medium (Gibco, Cat# 21103-049) supplemented with 0.5% N2 (100x ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with the secondary fluorescent antibodies Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) and Alexa Fluor® 568 Goat Anti-Mouse IgG (H+L) (Life Technologies). Ovine (Fig ...
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bioRxiv - Physiology 2023Quote: ... samples were further incubated with secondary antibodies (donkey anti-rabbit IgG H+L Alexa Fluor 546 and donkey anti-goat IgG H+L Alexa Fluor 488, both at 1:1000, Thermo Fisher Scientific, USA) at 4 °C overnight and mounted with fluorescence mounting medium (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... specific antibody binding was visualized with Alexa Fluor 488 goat anti-rabbit IgG (H+L) and Alexa Fluor 546 goat anti-mouse IgG (H+L) (Life Technologies, CA, USA) in 1% BSA in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was measured by quantitative real real-time polymerase chain reaction (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with primers and probe specific for the CCHFV L gene.
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bioRxiv - Evolutionary Biology 2021Quote: ... we harvested virus from each virus line under sterile conditions by collecting up to 10 virus killed cadavers per line and transferring these to sterile 15 mL disposable tissue grinders (ThermoFisher Scientific, U.S.A.). Infected larvae were recognizable by their opaque ...
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bioRxiv - Immunology 2021Quote: ... Total mRNA was extracted from 190 pMHC-tetramerpos purified (Supplementary Figure 1A) virus-specific T-cell populations(37) using magnetic beads (Dynabead mRNA DIRECT kit; Invitrogen, Thermo Fisher Scientific). Ten μl (~1μg ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A common virus stock was generated by transfection of plasmid DNA into NLFK cell culture using Lipofectamine 2000 (Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... ZHBTc4 cells were transduced with pLV-pCAGGS-Tir1-V5 and PLEX-mCherry-OCT4-AID packaged lentiviral vectors (i.e Tir1-V5 and mCherry-Oct4-AID virus, respectively) and were selected with 2 μg/ml Puromycin (Thermo Fisher Scientific #A1113803) for 10 days ...
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bioRxiv - Microbiology 2020Quote: Proteins were harvested four to five days post virus infection in RIPA Lysis and Extraction Buffer (Boston Bio) with 1X Protease Inhibitor and 1X Phosphatase Inhibitor (Thermo Fisher Scientific). Protein Concentration was determined using BCA Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... cells (in-house stock regularly tested to be virus- and mycoplasma-free) were grown at 25°C in S2 cell media supplemented with 10% fetal bovine serum (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... the extracellular RSV virus were collected by PEG6000 precipitation method and intracellular RSV virus were collected by lysing the macrophage cells with the lysis buffer from mirVanaTM miRNA Isolation Kit (Ambion, Life Technologies, AM1560). Total RNA from RSV infected macrophage cells as well as stock virus was extracted using mirVanaTM miRNA Isolation Kit according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Purity and titer of virus were then assessed by SDS-PAGE and SYPRO ruby staining (S-12000, Life technologies, Carlsbad, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... For the preparation of the BGLF2-KO virus, HEK293/EBV(dBGLF2) cells (Konishi et al., 2018) were transfected with BZLF1 expression plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and quantitative real-time polymerase chain reaction (qRT-PCR) was conducted using TaqMan Fast Virus 1-Step Master Mix (Thermofisher Scientific, US) with primers and probe specific for the SARS-CoV-2 E gene following guidelines by the World Health Organization (https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assayv1991527e5122341d99287a1b17c111902.pdf ...
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bioRxiv - Microbiology 2021Quote: ... HCV RNA was quantified by one-step reverse transcription-quantitative PCR using 50 ng of total intracellular RNA and TaqMan® Fast Virus 1-Step Master Mix (Applied Biosystems) with primers and probe targeting the HCV 5’ nontranslated region as described previously [82] ...
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bioRxiv - Microbiology 2022Quote: The ZEBOV RT-PCR assay was performed in a 25 µL reaction mixture containing TaqMan Fast Virus 1-step master mix (Thermo Fisher Scientific), 5 µl template RNA ...
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bioRxiv - Microbiology 2019Quote: ... total RNA was reverse transcribed using virus-specific RT primers or oligo-dT primers and the SuperScript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA). Real-time quantitative PCR was performed on the Applied Biosystems 7500 system with QuantiTect SYBR Green PCR Master Mix (Qiagen ...
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bioRxiv - Neuroscience 2019Quote: cDNA sequences from the leader through the first half of the rabies virus P gene were amplified using Platinum SuperFi Green Master Mix (Invitrogen (Thermo Fisher), USA ...
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bioRxiv - Microbiology 2020Quote: ... apical poles were gently washed with warm PBS and then infected with a 150-μL dilution of HRSV-A (Long) virus in OptiMEM medium (Gibco, ThermoFisher Scientific) at a MOI of 1 ...
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bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... One microgram of RNA was reverse transcribed into cDNA using oligo-dT and MMLV-RT (Moloney Murine Leukemia Virus Reverse Transcriptase, Invitrogen, Thermo Fisher Scientific). Before cDNA synthesis ...
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bioRxiv - Neuroscience 2019Quote: ... Patient KS1-1 was consented for stem cell derivation and cells were reprogrammed using non-integrating Sendai virus vectors (CytoTune-iPS 2.0, ThermoFisher Scientific, Waltham, MA). 5 days post-induction ...
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: Viral RNA for real-time PCR assay was extracted from 140 ml of virus isolate (previously tittered as described above) and serum samples using Invitrogen PureLink RNA Mini Kit (Invitrogen, Valencia, CA) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cell supernatants were harvested at 48 h post-infection and virus titers were determined by infecting TZM-bl cells and measuring β-galactosidase activity using Galacto-Lite system (Applied Biosystems). For flow cytometry quantification of infection ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Immunology 2021Quote: ... T2 target cells and Epstein-Barr Virus-transformed B lymphoblastoid cell lines (EBV-LCLs) cells were cultured in RPMI-1640 (Thermo Fisher Scientific) supplemented with 10% FCS and 1% penicillin/streptomycin (#P433 ...
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bioRxiv - Immunology 2020Quote: ... at a concentration to give 100 to 250 foci per well in the virus only control wells was mixed 50:50 in 1% FCS MEM with 1 x antibiotic/antimycotic (Gibco, 15240-062) with serum doubling dilutions from 1:20 to 1:640 (or higher dependent on antibody levels ...
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bioRxiv - Immunology 2020Quote: ... Virus heat inactivation at 56 °C for 30 minutes and total RNA including virus RNA extraction was performed using TRIzol™ Plus RNA Purification Kit (ThermoFisher, USA). cDNA synthesis was performed using GoScript™ Reverse Transcription System (Promega ...
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bioRxiv - Immunology 2020Quote: ... pEco envelope was used to produce virus to infect murine T cells) using lipofectamine 2000 (Cat. No. 11668019, Invitrogen, Carlsbad, California, USA) on a 100□mm2 poly-D-lysine–coated plate (Corning ...
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bioRxiv - Microbiology 2021Quote: ... RNA extracted from animal samples or RNA standards were then measured using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, catalog # 4444432) and custom primers/probes targeting the E gene sgRNA (forward primer ...