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Citations for Thermo Fisher :
4301 - 4350 of 5189 citations for CD40L Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: The region from −2662 to −1 bp (relative to translation start site) of the human TMEM135 promoter was synthesized by Life Technologies. Additionally ...
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bioRxiv - Microbiology 2020Quote: The spike protein gene from MERS-CoV strain Camel/Qatar/2/2014 (GenBank accession number KJ650098.1) was codon optimized for humans and synthesized by GeneArt (Thermo Fisher Scientific). The synthesized S gene was cloned into a transgene expression plasmid comprising a modified human cytomegalovirus immediate early promoter (CMV promoter ...
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bioRxiv - Genetics 2021Quote: The human lung epithelial cell line Calu-3 (UC Berkeley Cell Culture Facility) was maintained RPMI media supplemented with Glutamax (Thermo Fisher), 10% FBS (HyClone) ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Genomics 2021Quote: Human embryonic kidney 293T (HEK293T) cells were cultured in DMEM media (TH.Geyer, L0102) supplemented with 10% FBS (Thermo Fisher, 10500-064) and 100 U/ml Penicillin and 100 μg/ml Streptomycin (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Immunology 2021Quote: ... with unconjugated rabbit anti-human phosphorylated NF-κB p65 (Clone: Ser536 93H1) followed by goat anti-rabbit IgG (H+L) Alexa Fluor 488 (Thermo Fisher). For p38 ...
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bioRxiv - Immunology 2021Quote: ... and either left them untreated (hence naive) or activated them ex vivo with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific), wherein the bead-to-cell ratio was 1:1 ...
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bioRxiv - Immunology 2021Quote: ... at a concentration of 1×105 cells/100μl of complete medium with or without Human T-Activator CD3/CD28 Dynabeads® (ThermoFisher Scientific). NAFs/CAFs ± SI were added to wells of lymphocytes at a concentration of 1×104 cells/100μl (ratio of 1:10 NAFs/CAFs:lymphocytes ...
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bioRxiv - Microbiology 2021Quote: ... and the human microglial cell line HMC3 (ATCC® CRL-3304™) were cultured in Dulbecco’s Modified Eagle Medium (10566016, Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FCS ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells (authenticated by ATCC through their human STR profiling cell authentication service) were maintained in high glucose DMEM with Glutamax (Gibco 31966021), supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes 45 using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated using the Ion AmpliSeq™ Transcriptome Human Gene Expression Core Panel and Chef-Ready Kit (Thermo Fisher Scientific), according the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The raw spectra were searched against the Human (Homo sapiens) reference proteome database (UNIPROT) using SEQUEST HT (Proteome Discoverer v1.4, Thermo Fisher Scientific) with a Full MS precursor mass tolerance of 20 ppm and MS2 peptide fragment mass tolerance of 0.5 Da ...
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bioRxiv - Bioengineering 2020Quote: CD4+ T cells were electroporated prior to stimulation with anti-CD3/CD28 beads (Dynabeads Human T-Activator CD3/CD28, Thermo Fisher Scientific (ThermoFisher), Waltham ...
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bioRxiv - Bioengineering 2020Quote: Cytokine analysis was performed on supernatants derived from cultures given the indicated treatments using a human cytokine 10-plex panel (Thermo Scientific) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... human muscle biopsies from healthy donors were minced and digested in 0.8% w/v collagenase I (Life Technologies; Carlsbad, CA, USA) in DMEM ...
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bioRxiv - Neuroscience 2021Quote: The medial and baso-lateral amygdaloid nuclei were dissected from the mesial superior temporal lobe for 0.75-1 cm thick coronal slabs of frozen human brains on dry ice using a stainless steel punch (8mm diameter Thermo Fisher Scientific Integra Miltex standard biopsy punch) ...
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bioRxiv - Neuroscience 2021Quote: ... Anthony White (QIMR Berghofer Medical Research Institute, Australia) were maintained on human recombinant vitronectin in StemFlex medium (Life Technologies, cat. # A3349401). Differentiation was performed as previously described [15 ...
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bioRxiv - Neuroscience 2021Quote: ... 600 ng total RNA of human primary mesenchymal stem cells were reversely transcribed using the RevertAid RT Reverse Transcription Kit (K1691, Thermo Scientific) according to the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: ... and Human Embryonic Kidney 293 (HEK) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies- Invitrogen, Carlsbad, CA, USA). Both media were supplemented with 10% Fetal Bovine Serum Gold (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... and Human Embryonic Kidney 293 (HEK) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies- Invitrogen, Carlsbad, CA, USA). Both media were supplemented with 10% Fetal Bovine Serum Gold (FBS ...
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bioRxiv - Cell Biology 2021Quote: Human PCNT siRNA (Smart Pool) (M-012172-01-0005; Dharmacon) was transfected into cells with Lipofectamine RNAi MAX (13778100; ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Human Embryonic Kidney (HEK293T) cells were cultured in Dulbecco’s modified Eagle’s Medium–Glutamax medium (DMEM) (Gibco, Waltham, MA, USA, #6196-026) 10% FBS at 37°C and 5% CO2 with renewal of culture medium three times a week.
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bioRxiv - Cell Biology 2021Quote: ... N-terminal FLAG-tagged human MCC (P23508-1) constructs (pCMV2B vector) were transfected into HEK293 cells and selected with G418 (Gibco #10131035). G418-resistant cells were grown ...
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bioRxiv - Cell Biology 2021Quote: ... EpiLCs were induced by plating 1.5 x 106 ESCs on a 10 cm dish coated with human plasma fibronectin (16.7 mg/ml) in the 1:1 mix of DMEM/F12 (Gibco, 11302-33) and Neurobasal medium containing N2 supplements ...
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bioRxiv - Microbiology 2020Quote: ... The resulting plasmid DNA construct was used to transfect Expi293F human cells using the Expi293F transfection kit and Expi293F expression medium (Thermo Fisher). Cells were harvested by centrifugation at 1,000 g for 30 min and resuspended in 1 x PBS supplemented with 30 mM imidazole ...
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bioRxiv - Systems Biology 2020Quote: ... 72 h after seeding on 96-well microplates differentiated cells were incubated with recombinant human interferon gamma (IFN-γ, ThermoFisher, #PHC4031) at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10 ...
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bioRxiv - Microbiology 2020Quote: ... A549 (human lung carcinoma) cells were used for infection experiments and were maintained in Ham’s F12 nutrient mixture (Thermo Fisher Scientific) with 8.3% FBS.
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bioRxiv - Microbiology 2020Quote: ... Goat anti-Rabbit IgG Alexa Fluor 647 and Goat anti-Human IgG Alexa Fluor 647 were used at 1ug/ml concentration in FACS buffer (ThermoFisher Scientific). For western blotting ...
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bioRxiv - Evolutionary Biology 2020Quote: ... a formulation optimal for human fibroblasts [(Dickson et al., 2000): 1:1 (vol/vol) medium M199 and medium M106 (ThermoFisher Scientific) + 15% BCS + 10 ng/ml EGF + 0.4 ug/ml hydrocortisone + 1% Penicillin-Streptomycin] ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes(Rihn et al., 2021) using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... A549 cells (human, lung; CRM-CCL-185, ATCC) were cultivated in DMEM/F-12 medium with Nutrient Mix (Thermo Fisher Scientific) supplemented with 10% FCS ...
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bioRxiv - Molecular Biology 2020Quote: ... Lentiviral overexpression of PNUTS was achieved by cloning the full-length human cDNA of PNUTS into pLenti4/v5-DEST (Life technologies). The PP1 non-binding mutant PNUTS (W401A ...
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bioRxiv - Microbiology 2021Quote: ... sodium bicarbonate and either a) 10% heat-inactivated A+ human plasma (Interstate Blood Bank, Memphis, TN) or b) 1% (w/v) AlbuMAX II (Thermo Fisher) and 320 μM hypoxanthine (Sigma) ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Microbiology 2021Quote: ... ACE2 expression was assessed by staining cells with polyclonal rabbit antibodies against human ACE2 (PAB886Hu01, Cloud-Clone Corp.) followed by secondary anti-rabbit antibodies conjugated with PE (1:250, # P-2771MP, ThermoFisher Scientific). Samples were analyzed on CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... 3×105 live cells were incubated with the serum from a convalescent donor at the 1:100 dilution in PSB for 30 min followed by the incubation with secondary anti-human IgG antibodies conjugated with PE (1:250, # H10104, ThermoFisher Scientific) for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Cytokine and chemokine levels in mycobacterial-infected supernatants were determined using human cytokine and chemokine and growth factor 45-plex panel 1 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Human cavin3 Stealth siRNAs (set of 3-HSS174185, 150811, 150809) and Human BRCA1 Stealth siRNAs (set of 3 - HSS101089, 186096, 186097) were purchased from Life Technologies Australia Pty Ltd ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF-7 human breast carcinoma cells (ATCC, Rockville, MD) were grown in Dulbecco’s MEM (DMEM) with Glutamax-1 (Gibco, Paisley, Scotland) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... The human pancreas adenocarcinoma cell line Bx-PC3 obtained from ATCC (ATCC CRL-1687) was grown in RPMI-1640 medium (ThermoFisher Scientific) supplemented with 10% fetal calf serum at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... envelope and one of the transfer plasmids were co-transfected into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 (ThermoFisher Scientific) according to the manufacturer’s lentiviral production protocol ...
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bioRxiv - Cell Biology 2021Quote: ... human female adenocarcinoma of the mammary gland) were maintained in Dulbecco’s Modified Eagle Medium High Glucose (ThermoFisher Scientific, #11-965-118), 10% foetal bovine serum (Bovogen ...
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bioRxiv - Cell Biology 2021Quote: ... Transferrin, Selenite) premix (resulting in 10 µg/mL insulin, 5 µg/mL human transferrin and 30 nM sodium selenite) (Life Technologies). One droplet (10 µL ...
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bioRxiv - Cell Biology 2020Quote: ... coated overnight with 10 μg/ml of xeno-free human recombinant Laminin 521 (LN521, Biolamina) prepared in GMP DPBS+/+ (A1285801, Life Technologies) by reversing the strainer and washing the EBs into the plate with GMP N2 media (CTS™ DMEM-F12 ...
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bioRxiv - Biophysics 2021Quote: Human wild type ABCG2 or ABCG2 R184A containing an N-terminal Flag-tag was expressed in HEK293-EBNA (Thermo Fisher Scientific) cells as previously described19 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Non – metastasis human colon cancer cell line (HT-29) were cultured in Dulbecco’s modified Eagle’s medium (DMEM: Gibco, Thermo Fisher, USA) and metastasis human colon cancer cell line (SW-620 ...