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Citations for Thermo Fisher :
4551 - 4600 of 5189 citations for CD40L Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... (74) were cultured in keratinocyte serum-free media (KSFM) supplemented with bovine pituitary extract and human epidermal growth factor (Thermo Fisher). HCT116 human colon carcinoma lines were obtained from ATCC and maintained following their recommendations ...
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bioRxiv - Microbiology 2023Quote: The African green monkey kidney Vero E6 (ATCC, no. CRL-1586) and human Calu3 cells (ATCC, no. HTB-55) were grown in DMEM medium (Thermo Fisher) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... purified CD4+CD25+CD127lo naive Treg cells (5 × 104) were stimulated with Dynabeads Human T-activator CD3/CD28 (12.5 μl/ml) (Thermo Fisher Scientific) and IL-2 (100 U/ml ...
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bioRxiv - Microbiology 2023Quote: HeLa epithelial cells and MelJuSo human melanoma cells were cultured in Gibco Iscove’s Modified Dulbecco’s Medium (IMDM; ThermoFisher Scientific, the Netherlands) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: ... harboring a human APP transgene with the K670N and M671L Swedish mutations (25) were grown in DMEM/F12 medium (Thermo Scientific) supplemented with 5% FBS ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed three times with flow staining buffer and labeled with a PE-conjugated anti-Human IgG Fc antibody (Thermo Fisher) for 30 mins ...
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bioRxiv - Cell Biology 2023Quote: ... These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies). The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Methods used for these assays have been described.28 Briefly, human FLAG-tagged CDKL5 WT or kinase dead (KD, CDKL5 K42R) constructs were subcloned into pT7CFE1-CHis plasmid (Thermo Fisher). These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Bioengineering 2023Quote: ... wells were washed 3 times with PBS with 0.1% tween (PBST) and then incubated overnight at 4 °C with a human hemoglobin-FITC conjugated antibody (Life Technologies, A80134F). Samples were washed three times with PBST prior to imaging on a Keyence BZ-X800 microscope.
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bioRxiv - Cell Biology 2023Quote: Human placental–epithelial choriocarcinoma cells (JEG-3 HTB-36TM ATCC®) were cultured in Earle’s Minimum Essential Medium (EMEM) (Gibco™) containing 2-mM L-glutamine supplemented with 1% nonessential amino acids (Gibco™) ...
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bioRxiv - Cell Biology 2023Quote: Mouse embryonic fibroblast cells (MEFs) and Human embryonic kidney 293T (HEK293T) were cultured in Dulbecco’s modified Eagle medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... expressing GFP-LC3A (human), were prepared as described previously (Fletcher et al., 2018; Jacquin et al., 2017) and cultured in DMEM/F12 (Gibco, 11320074) containing 5% horse serum (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Hela and 293T cells (Human Embryonic Kidney, HEK293T) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (GIBCO, Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2023Quote: ... Hela and 293T cells (Human Embryonic Kidney, HEK293T) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (GIBCO, Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2023Quote: ... PIK3CA/PIK3R1 (p110a/p85a) recombinant human protein for use in the experiment shown in Figure 4F was purchased from Thermo Fisher Scientific (catalog number PV4788).
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bioRxiv - Neuroscience 2022Quote: ... Preparation of nuclear and cytoplasmic extracts from human fibroblasts was performed using a NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher), as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... except for RWPE1 which were maintained in Keratinocyte Serum Free Medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (manufacturer supplied, Life Technologies). For spheroid cultures ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Previously homogenized human-derived AD crude brain extracts were diluted 1 to 20 with Opti-MEM (Thermo Fisher Scientific, cat. 31985062) and sonicated in a Qsonica multiplate horn water bath for 3 minutes at 40% power ...
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bioRxiv - Immunology 2023Quote: Serum samples were analyzed using the Immune Monitoring 65-Plex Human ProcartaPlex Panel (Cat# EPX650-16500-901; ThermoFisher Scientific, Vienna, Austria), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: The human alveolar epithelial cell line A549 (ATCC CCL-185) was cultured in F-12K culture medium (Gibco™ 21127-030) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2023Quote: ... human prostate tissues were first digested in aDMEM/F12/Collagenase II (1.5mg/ml)/Hyaluronidase VIII (1000 u/ml; Thermo Fisher Scientific) plus 10 μM Y-27632 (Tocris ...
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bioRxiv - Immunology 2023Quote: Cryopreserved/thawed PBMCs were stained for 30 minutes at 4°C with the following reagents or conjugated anti-human mAbs: Live/Dead Aqua (Life Technologies), anti-CD4-PerCP (L200 ...
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bioRxiv - Cancer Biology 2023Quote: H357 and H376 cells at ∼80 % confluence were treated either with distilled water (as control) or with 20 ng/ml of Human Recombinant FGF2 (PHG0026, ThermoFisher Scientific) for 48 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Human liver homogenate was prepared from human FLC or matched normal liver tissue (adjacent to the FLC) using RIPA Lysis buffer (Thermo Fisher) with Halt protease and phosphatase inhibitor cocktail (Thermo Fisher) ...
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bioRxiv - Pathology 2023Quote: ... 25 nM) or Human YAP/TAZ-siRNA (UGUGGAUGAGAUGGAUACA) (102) by using Lipofectamine RNAiMAX reagent (13778075, Thermo Fisher Scientific, Waltham, MA, USA). Cells were harvested after 72 h.
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bioRxiv - Microbiology 2023Quote: Human ileocecal adenocarcinoma cells (HCT-8; ATCC CCL-244) were cultured in RPMI 1640 ATCC Modification medium (Thermo Fisher A14091-01) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The experiments were normalized using human β-Actin as the endogenous control (FAM/MGB probe, cat. no. 401846; Applied Biosystems, USA).
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bioRxiv - Bioengineering 2023Quote: Human kidney epithelial cell line HEK293T was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units/ml Pen-Strep (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: Human lung Carcinoma cell line A549 was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units /ml Pen-Strep (GIBCO ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies and dilutions were used: Alexa Fluor 568- and 647-conjugated goat anti-human and anti-rabbit (Life Technologies), 1:1000 ...
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bioRxiv - Bioengineering 2023Quote: Human lung Carcinoma cell line A549 was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units /ml Pen-Strep (GIBCO ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
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bioRxiv - Molecular Biology 2023Quote: The surface of all cultureware used for the maintenance and propagation of human pluripotent stem cells (WTC-11 line) was coated with Corning Matrigel (Fisher Scientific) prior to cell seeding ...
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bioRxiv - Genetics 2023Quote: ... 293T human embryonic kidney epithelial cells (ATCC, #CRL-3216) were grown in complete Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher, #11995-065) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... we used quantitative TaqMan based OpenArray Human MicroRNA Panel (Cat# 4470187; Thermo Fisher Scientific, Life Technologies Corporation, Grand Island, New York). This panel contains 754 well-characterized human miRNA sequences from the Sanger miRBase v14 ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding paired Ig heavy and light chains were co-transfected into human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) for recombinant mAb production ...
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bioRxiv - Immunology 2023Quote: ... Serum starved PANC-1 and MiaPaCa-2 cells were incubated with human properdin (20 µg/ml) and CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (5 μM ...
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bioRxiv - Systems Biology 2023Quote: ... IgG1 and IgG4 in the basolateral media were quantified using human ELISA) IgG1 and IgG4 quantification kits (Invitrogen, EHIGG1 and BMS2095) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... were added as the primary antibodies and HRP-labelled goat anti-human IgE antibody (at 1:1,000 dilution, Invitrogen, Waltham, MA) was used as the secondary antibodies ...
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bioRxiv - Immunology 2023Quote: Polyclonal T cells were isolated from PBMCs (see above) and pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron) ...
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bioRxiv - Developmental Biology 2023Quote: 100 ng total RNA was reverse transcribed using the Ion AmpliSeq Transcriptome Human Gene Expression Kit following the manufacturer’s protocol (Thermo Fisher Scientific). Target genes (SQSTM1 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature and then exposed to antibodies specific for human PD-1 (ThermoFisher Scientific; 14-9969-82), Iba1 (Wako Chemicals ...
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bioRxiv - Cell Biology 2023Quote: MRC5 fibroblasts (ATCC cat. no. CCL-171) and adult human mesenchymal cells were cultured in DMEM (cat. no. 11965092, Thermo Fisher) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: Saturating amounts of recombinant human (rh) EGFR-Biotin and rhPD-L1-Biotin (ACRO Biosystems) were coated onto separate Streptavidin-coated DynaBeads (Thermo Fisher) using manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
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bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and processed for quantitative PCR analysis of SMAD3 and SP1 binding to the human MCEMP1 promoter using Power SYBR Green PCR Master Mix (ThermoFisher Scientific). ChIP-qPCR results were calculated using the ΔΔCt method and are shown as percentage of input DNA for each ChIP experiment.