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Citations for Thermo Fisher :
4251 - 4300 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected as follows: 400 µg MaxiPrepped recombinant DNA (pcDNA3.1_Strep-Strep-TEV-hPADI6) was diluted in 20 mL Opti-MEM® (Gibco™, #11058021), 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences ...
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bioRxiv - Microbiology 2024Quote: ... The endotoxin content of all recombinant antigens was measured using the Limulus amebocyte lysate (LAL)-based Chromogenic Endotoxin Quantitation Kit (Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The mouse was immunized with 50 mg of recombinant hUNG-flRBM-C2 in Imject™ Alum Adjuvant (Thermo Scientific, Catalogue #. 77161). The primary immunization was through IP (Peritoneal injection) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then transfected overnight with 100 pmol of tracrRNA and crRNA complexed with 4 pmol recombinant cas9 protein using RNAiMAX (Thermo Fisher). Medium was then replaced and cells were grown for 48 h before assessing fluorescence and sorting.
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bioRxiv - Microbiology 2019Quote: ... Eluted proteins were analyzed by SDS-PAGE followed by Coomassie-staining or western blot using an anti-His primary antibody (Thermo Fisher Scientific, Waltham, MA, USA), HRP-conjugated secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... THP-1 cells were expanded in RPMI medium (ATCC® 30-2001™) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific, Massachusetts, USA), 100 U/ml penicillin and 100 μg/ml streptomycin sulfate (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted protein was then incubated with purified 6x-His-TEV protease E106G (Cabrita et al. 2007) and 500 μM TCEP (Thermo Fisher Scientific, product number 77720) for 20-24 hours ...
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bioRxiv - Microbiology 2021Quote: ... Standard Western blotting procedures were used with the following antibodies: HIV-1 gp120 (NIH AIDS Reagent program catalog no. 288) and 6x-His Tag (ThermoFisher catalog no. MA1-21315-HRP).
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 Spike RBD, His, Avitag™ (ACRO Biosystems, SPD-C82E9) immobilized on paramagnetic beads (Dynabeads M-280 streptavidin; Invitrogen Dynal AS, Oslo, Norway) as target antigen ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the modified pSec-NtermHis6 vector containing secretory signal peptide (IgK Leader) as found originally in commercial vector pSecTag/FRT/V5- His-TOPOR vector (Thermo Fisher Scientific, Waltham, MA, USA), but fused to six histidine residues (His6 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed once with 2% HI-FBS S-MEM and further stained with PE-conjugated anti-mouse IgM (Thermo Fisher Scientific, #12-5790-81) for 30 minutes to detect the HTII-280 primary antibody ...
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bioRxiv - Microbiology 2020Quote: Toxicity of the surfaces was tested on human immortalized HaCaT keratinocytes (ThermoFisher). Cells were maintained in DMEM high glucose medium supplemented with Na-pyruvate (1mM) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T (Human Embryonic Kidney cells 293) cells were obtained from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 293T and human secondary fibroblasts cells were cultured in DMEM (Gibco, 11995) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... in tissue culture plates coated with 2.5μg/cm2 human fibronectin (Thermo Fisher). HEK293T cells and L cells were cultured in DMEM with GlutaMAX supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... HCT116 cells (human colorectal epithelial) were maintained using DMEM (Gibco, 41966-029) supplemented with 10% FBS (Sigma ...
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bioRxiv - Immunology 2021Quote: Human interleukin two (IL-2) Ready-SET Go! ELISA kit (eBioscience/Invitrogen) and Nunc MaxiSorp 96-well plates (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Human iPS WT-11 cells were cultured on Vitronectin (Thermo Fisher Scientific) coated 6-well plates or glass coverslips (for smRNA FISH purposes ...
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bioRxiv - Immunology 2021Quote: ... and Pacific Blue-conjugated mouse anti-human CD274 (ThermoFisher Scientific, MA, USA) mAbs ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated in goat anti-human HRP secondary antibody (ThermoFisher Scientific) at 1:10,000 dilution in blocking buffer for 45-60 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and against human CXCL4L1 (rabbit anti-CXCL4L1, PA5-21944, Invitrogen; 1:50). Samples were then prepared for microscopy using Duolink® mounting medium with DAPI ...
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bioRxiv - Biochemistry 2022Quote: ... 100 units/ml human LIF supplemented with 10% fetal bovine serum (Gibco). Cultures were grown in a humidified incubator at 37°C and 5% CO2.
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bioRxiv - Developmental Biology 2022Quote: ... Labelled DNA was then hybridized to Clariom S human GeneChips (ThermoFisher Scientific) and incubated at 45℃ in the GeneChip Hybridization oven 640 (Affymetrix ...
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bioRxiv - Genomics 2019Quote: ... The labeled DNA was then hybridized to Human Gene ST1.0 microarrays (Affymetrix), washed ...
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bioRxiv - Genomics 2020Quote: ... human Malat1 (positive control, Type 1 Probe, VA1-11317, Thermo Fisher Scientific), and bacterial DapB (negative control ...
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bioRxiv - Physiology 2020Quote: ... RNA isolation of human left ventricular biopsies was performed with TRIzol (Invitrogen/ThermoFisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-human-Alexa 488 or –Alexa 555 (Invitrogen cat #A11013 and #A21433) were used ...
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bioRxiv - Biochemistry 2019Quote: Human cell line HeLa were grown in DMEM (Invitrogen/ThermoFisher Cat #11965) supplemented with 10% FBS and 1X penicillin and streptomycin cocktail ...
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bioRxiv - Biochemistry 2019Quote: Human cell line HeLa were grown in DMEM (Invitrogen/ThermoFisher Cat #11965) supplemented with 10% FBS and 1X penicillin and streptomycin cocktail ...
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bioRxiv - Immunology 2020Quote: ... Cross-adsorbed goat anti-human IgG (H+L) secondary antibody (A18811, Invitrogen) was used at a dilution of 1:3,000.
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bioRxiv - Molecular Biology 2019Quote: ... Cells were stimulated with Dynabeads™ Human T-Expander CD3/CD28 (Gibco) 7 days prior to nucleofection ...
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bioRxiv - Molecular Biology 2019Quote: ... All immortalized human myoblasts were cultured in F10 medium (Gibco/ThermoFisher Scientific) supplemented with 20% FBS (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2019Quote: ... All immortalized human myoblasts were cultured in F10 medium (Gibco/ThermoFisher Scientific) supplemented with 20% FBS (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2019Quote: ... Human insulin-like growth factor 1 (IGF-1) was from Thermo Fisher and used as IGF-1 receptor activator.
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bioRxiv - Cell Biology 2019Quote: ... Human TERT-RPE cells were cultured in DMEM+F12 (1:1) (Invitrogen) containing 10% FBS ...
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bioRxiv - Bioengineering 2019Quote: ... activated using Dynabeads Human T-Activator CD3/CD28 beads (Gibco, Life Technologies), and cultured in X-VIVO 15 (Lonza Cat# BE02-053Q ...
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bioRxiv - Bioengineering 2019Quote: ... activated using Dynabeads Human T-Activator CD3/CD28 beads (Gibco, Life Technologies), and cultured in X-VIVO 15 (Lonza Cat# BE02-053Q ...
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bioRxiv - Biochemistry 2021Quote: ... Human embryonic kidney 293T cells (HEK-293T) were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Genetics 2020Quote: ... and quantified using the Quantifiler Human DNA Quantification kit (Thermo Fisher Scientific) on an Applied Biosystems 7500 Real-time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 647 goat anti-human IgG (H+L) (Molecular Probes, A21445) (1:500 IF) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were activated using Dynabeads Human T-Activator CD3/CD28 beads (Invitrogen) according to the manufacturer’s instructions and cultured in RPMI supplemented with 10% FBS and 30 U/ml recombinant human IL-2 (PeproTech ...
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bioRxiv - Bioengineering 2021Quote: ... RBCs were incubated with anti-Human IgG Alexa Fluor 488 (Thermo Fisher) for 2 h ...
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bioRxiv - Bioengineering 2021Quote: Human-derived cell lines (HEK293FT and HeLa) were purchased from Invitrogen (R70007) and American Type Culture Collection (CCL-2) ...
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bioRxiv - Biochemistry 2020Quote: Human ASCT2 (hASCT2) was produced in Pichia pastoris X-33 strain (Invitrogen) in fermentor59 and purified using DDM and CHS (Anatrace ...
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bioRxiv - Biophysics 2021Quote: ... while HRP-conjugated anti-human IgG Fc (Invitrogen™, Waltham, MA, USA) diluted 1:10,000 in PBS was used in wells treated with REGN10987 ...
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bioRxiv - Microbiology 2021Quote: ... Alexa Fluor-647-conjugated goat anti-human IgG (H+L) Abs (Invitrogen) were used as secondary antibodies to stain cells for 30 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... the human CCKBR gene was cloned into a modified pFastBac1 vector (Invitrogen) with N-terminal HA signal sequence connected to a Flag-tag and C-terminal PreScission protease site followed by a 2 × Strep-tag ...
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bioRxiv - Biophysics 2021Quote: ... the human CCKAR gene was cloned into a modified pFastBac1 vector (Invitrogen), which contains an expression cassette with a hemagglutinin (HA ...