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Citations for Thermo Fisher :
4151 - 4200 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... along with goat anti-human IgG Fc secondary antibody (polyclonal, ThermoFisher). HLA-ESC was detected with an anti-HLA-E antibody (clone 3D12 ...
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bioRxiv - Immunology 2023Quote: ... using the Dynabeads Untouched Human CD4 T cell kits (Thermofisher, USA). T lymphoblast were generated by culturing T cells at 2 million/mL onto P12 wells on RPMI 1640 with FBS (10 %) ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human keratinocyte growth supplement (HKGS, Gibco™, cat. #S0015), containing Human growth factor I insulin-like recombinant ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 20 ng/ml human epidermal growth factor (Invitrogen, PHG0311), 20 ng/ml human basic fibroblast growth factor (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... and biotinylated human BCMA (Bio-connect) with streptavidin-PE (Thermo Fisher). For intracellular staining ...
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bioRxiv - Microbiology 2024Quote: ... followed by AlexaFluor 647-conjugated anti-human antibodies (Invitrogen, 1:1,000). Slides were then stained with 1 μg/mL DAPI and mounted in Citifluor (Citifluor Ltd. ...
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bioRxiv - Cell Biology 2024Quote: ... Human Pools Set v3.0 kit (Applied BiosystemsTM, Thermo Fisher Scientific, USA) and the TaqManTM MicroRNA Reverse Transcription Kit (Applied BiosystemsTM ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... Normal human epithelial cells were cultured in keratinocyte-SFM medium (Gibco) supplemented with 1% antibiotic-antimycotic (Gibco).
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bioRxiv - Microbiology 2024Quote: ... Alexa Fluor 647 conjugated anti-human IgG Fc secondary antibodies (Invitrogen) (1:1500 ...
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bioRxiv - Cell Biology 2024Quote: The following human (h) and rat (r) TaqMan assays (Thermo Fisher) were used ...
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bioRxiv - Cancer Biology 2024Quote: ... added with human complete organoid medium containing Advanced DMEM/F12 (GIBCO), 10 mM HEPES (GIBCO) ...
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bioRxiv - Neuroscience 2024Quote: ... miBrains were cultured in Human Endothelial Serum-Free Medium (ThermoFisher Scientific) with 1% pen/strep (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... human custom-made 3’UTR HEC1 siRNA (oligo #3, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit anti-Human IgG DyLight™ 800 (Cat# SA5-10116, Thermofisher). For the appraisal of common painkillers drugs were as follows ...
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bioRxiv - Developmental Biology 2024Quote: ... Complete human medium with 10% exosome depleted FBS (Gibco, Massachusetts, US) was replenished and device placed into incubator ...
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bioRxiv - Immunology 2024Quote: ... and human IL-6 (Gibco, PeproTech, #200-06; 20 ng/mL). Prostaglandin E2 (PGE2 ...
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bioRxiv - Systems Biology 2024Quote: ... Human THP-1 monocytes were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... and cytokine levels were measured using Human Uncoated ELISA Kits (ThermoFisher) targeting IFNψ ...
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bioRxiv - Molecular Biology 2024Quote: Human MET knockout HeLa cells were transiently transfected with Lipofectamine3000 (Invitrogen) according to the manufacturer’s protocols in a 6-well plate format ...
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bioRxiv - Immunology 2024Quote: ... stimulated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco™) and cultured for 5 days in AIMV medium supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Genomics 2024Quote: We used the human Amyloid-β 40 (ThermoFisher Cat No. KHB3481), Amyloid-β 42 (ThermoFisher Cat No ...
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bioRxiv - Plant Biology 2019Quote: ... Aliquots were analyzed on a 10% SDS-PAGE followed by Coomassie Blue staining or Western blot analysis with goat α-6X-His-HRP antibody (Life Technologies, Carlsbad, CA) at a 1:5,000 dilution in Tris-buffered saline (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The gene optimized for the Drosophila codon usage and extended by C-terminal His-tag was purchased from Life Technologies (DNA String fragments, USA). The DNA fragment was inserted into a pMT-BiP-V5-His_A vector (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: 1-2 μg of purified 6xHis-tagged calcineurin or 6xHis-MBP as a negative control was first bound to 10 μL magnetic Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Fisher Sci., 10104D) in 490 μL base buffer (50 mM Tris-HCl pH 7.5,150 mM NaCl ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by sequencing using the Ion PI™ Hi-Q™ Sequencing 200 Kit on Ion Proton PI™ V2 chips (Thermo Fisher Scientific), an Ion Proton™ System ...
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bioRxiv - Genetics 2020Quote: ... in all the above clonings except for the his-72::GFP repair template which required ccdB resistant bacteria for which we used One Shot ccdB Survival (Thermo Fisher Scientific, cat. A10460). DNA extractions by miniprep were done with the ZymoPURE Plasmid Miniprep kit and elution with water (Zymo Research ...
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bioRxiv - Immunology 2020Quote: ... Nunc MaxiSorp ELISA plates were coated with 50 μL of capture antibody (anti-6x-His antibody clone HIS.H8; 2 μg/mL; Thermo Fisher Scientific, Waltham, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR product of the C-His oipA gene fragment was cloned into vector pLATE11 using aLICator LIC Cloning and Expression Kit 1 (Thermo Fisher Scientific, United States) by following the kit’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Vero/hSLAM and VeroE6/TMPRSS2 were maintained in supplemented DMEM with 10% HI-FBS and 1 mg/mL G418 sulfate (Thermo Fisher 10-131-027). HeLa/ACE2/TMPRSS2 cells were a generous gift from Dr ...
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bioRxiv - Microbiology 2023Quote: ... Protein bands were visualized with Coomassie staining (ROTIBlue Quick, Carl Roth) or immunodetected with a monoclonal anti-His antibody conjugated to HRP (MA1-21315-HRP, Thermo Fisher, 1:2000 diluted). A detailed protocol can be found in protocols.io (dx.doi.org/10.17504/protocols.io.bu67nzhn).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% (v/v) heat-inactivated (HI) FBS (Thermo Fisher Scientific, Waltham, MA, USA). Stable cell lines expressing F-CIC-S ...
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bioRxiv - Pathology 2023Quote: ... The cDNA was used for RT-qPCR with qPCRBIO SyGreen Blue mix Hi-ROX (PCR Biosystems, London, UK) on the StepOne Plus Real-Time PCR machine (Applied Biosystems, Foster City, CA) and primers listed in Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The TMT10-plex-tagged sample (1 µg) was loaded onto an Acclaim PepMap100 C18 trap column (Thermo Fisher Scientific, 100 µm × 2 cm, 100 Å, 5 µm) at 5 µL/min using an UltiMate 3000 RSLCnano system (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: The pFAST-bac1vectors were used to produce recombinant baculovirus according to the protocol for the Bac-to-Bac system (Life Technologies). Recombinant GST-A3H Hap I WT and mutant baculovirus (K117E ...
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bioRxiv - Cell Biology 2020Quote: ... cleared lysates were mixed with Protein G/A (50:50) recombinant protein G agarose beads (catalog #15920-010, Invitrogen, Carlsbad, CA) coupled with anti-HA (catalog #3724S ...
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bioRxiv - Genetics 2021Quote: ... using 2 µg of total RNA in the presence of 2 U/µl of RNaseOut Recombinant RNase Inhibitor (Thermo Fisher Scientific). Genomic DNA was eliminated using the RapidOut DNA Removal Kit (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Recombinant bacmids were made according to instructions for the Bac-to-Bac® Baculovirus expression system given by the manufacturer (Invitrogen) using DH10EMBacY (Geneva Biotech) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Plasmid DNAs were isolated according to standard protocols and recombinant plasmids were subjected to sequencing using universal M13 primers and the BigDye terminator cycle sequencing kit v1.1 (Thermo Fisher Scientific). Sequencing products were EDTA/ethanol-precipitated ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant lentivirus was used to infect NIH3T3 cells and stably expressing cells selected with 5 μg/ml puromycin (Thermo Fisher, A1113803). Conditional expression of CB1R was induced by the addition of Doxycycline (Millipore Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... and further digested for 5 min at room temperature (22°C) using a TrypLE Express recombinant protease (ThermoFisher Scientific, cat. #12605010). The dissociated cells were sedimented by centrifugation (900 g for 10 min at 4°C) ...
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bioRxiv - Molecular Biology 2021Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was incubated with superparamagnetic beads covalently linked to recombinant protein G (Dynabeads protein G Novex, Life technologies, USA) to obtain the precleared lysate ...
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bioRxiv - Developmental Biology 2019Quote: ... Recombinant HSF2 (TP310751 Origen) was desalted (ZebaTM Spin Desalting Columns, 7K molecular-weight cutoff, 0.5 ml (1034–1164, Fisher Scientific, Germany)) and biotinylated at a molar ratio biotin/protein (3:1 ...
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bioRxiv - Cell Biology 2019Quote: ... sequence-verified plasmids (wild-type and mutant variants of pbiG2ab:6His-ZZ-SNAPf-DHC1/IC2/LIC2/Tctex1/Robl1/LC8) were used to generate recombinant baculoviral genomes by Tn7 transposition into DH10Bac cells (Life Technologies). White ...
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bioRxiv - Developmental Biology 2019Quote: ... was introduced into the BglII and SpeI sites of pCR4-HBB-24XMS2SL and the recombinant HBB- 24XMS2SL-MCS sequence was subcloned into the pcDNA3 mammalian expression vector (ThermoFisher Scientific) using the NotI and XbaI sites ...
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bioRxiv - Biochemistry 2021Quote: Recombinant baculoviruses for expression of the mTTYH3 ECD1-T4L-ECD2 construct were generated using the Bac-to-Bac system (ThermoFisher Scientific). Sf9 cells were cultured at 27 °C in 1 L culture flasks with ESF-921 insect cell culture medium (Expression systems) ...