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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for Recombinant Human ITGAV & ITGB5 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... We then amplified the library using Hi-fidelity KAPA PCR kit (KAPA) for 6 cycles and quantified the library using Qubit high sensitivity DNA assay (Thermofisher). We performed QC using bioanalyzer (Agilent ...
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bioRxiv - Microbiology 2021Quote: The SARS-CoV-2 NTD (residues 13-303) or RBD (residues 319-541) with a C-terminal His tag was cloned into a modified pFastBac vector (Invitrogen) that encodes a melittin signal peptide before the NTD or RBD ...
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bioRxiv - Immunology 2020Quote: ... containing the gp67 secretion signal peptide and a C-terminal 6×His tag was inserted into pFastBac-Dual vectors (Invitrogen) and transformed into DH10Bac component cells ...
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bioRxiv - Microbiology 2021Quote: ... VF-Hi and VF-Lo (generated from this study) were maintained in Dulbecco′s Modified Eagle′s Medium (DMEM) (Gibco) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... which were subsequently purified and ligated upstream the His-tag into the expression vector pTrcHis2 (Invitrogen™, cat. n° V36520). The recombinant vectors were transformed into the ΔarsC E ...
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bioRxiv - Genetics 2022Quote: ... The blasticidin resistance gene coding sequence was amplified by PCR with the primers BlasS SacII F1 and BlasS BamHI R3 from pcDNA6/V5-His-A (Invitrogen):
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was performed using the qPCRBio SyGreen Mix Hi-ROX (PCR biosystems) according to the manufacturer’s instructions and run on the Real Time PCR QuantStudio3 (ThermoFisher scientific) using the following conditions – Initial denaturation step at 95□ °C for 2 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant obtained after the centrifugation of the lysate at 9000 rpm for 25 minutes at 4°C was subjected to overnight binding with Hi-bind Ni-NTA agarose beads (Invitrogen). The beads were washed 4-5 times with wash buffer (20mM Tris ...
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bioRxiv - Cancer Biology 2019Quote: ... The PCa cells were maintained in F-12K media with 10% HI fetal bovine serum (FBS) (Gibco, Grand Island, NY). Once reaching 80-90% cellular confluence ...
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bioRxiv - Microbiology 2020Quote: ... fused to a Gly-Ser-Gly linker and a Myc epitope tag into the EcoRV site of pEF1 V5 His C (Invitrogen) using NEB HiFi Assembly Mix (New England Biolabs ...
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bioRxiv - Synthetic Biology 2020Quote: ... flow cytometry and in vivo binding assays) were subcloned via NcoI/EcoRI or HindIII/BamHI into either the pBAD/His A (Invitrogen) or pZA23MCS (EXPRESSYS ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and SC-His-Ura (for Ty1his3-AI) or YEPD + Geneticin (G418; 200 μg/ml for Ty1neo-AI) (ThermoFisher, Waltham MA) plates ...
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bioRxiv - Biochemistry 2020Quote: The C-terminal fragments of Stu2 used in the pulldown experiments were cloned into a pLIC vectors with a TEV-cleavable N-terminal 6-His tag and transformed into Bl-21 AI cells (Invitrogen). Cells were grown to an OD600 of 0.6 in TB media with 0.1% glucose and were induced by addition of IPTG and arabinose to 200 μM and 0.2% ...
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bioRxiv - Physiology 2021Quote: ... The resulting cell pellet was resuspended in hypotonic buffer and used for Western blotting using HRP-conjugated anti-His antibody (1: 10,000; Novex, Life technologies).
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bioRxiv - Biochemistry 2020Quote: ... the opsin-encoding constructs were fused in frame with a C-terminal 8-His tag and subcloned into the pPIC9K (A1ACR1, HfACR1 and AlACR2) or pPICZα (AlACR3) vector (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2019Quote: ... blocked with 5% milk in TBST and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (ThermoFisher MA1-21315, 1:3000). Blots were incubated with primary antibodies overnight at 4 °C with rocking and were then washed (3×5min ...
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bioRxiv - Cell Biology 2019Quote: ... and sequencing was performed on an Ion Proton system using with Ion PI Hi-Q sequencing kit (Thermo Fisher Scientific) the PI v3 chip (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane and analyzed by western blotting using an anti-His tag antibody (7) or stained with Krypton fluorescent dye (ThermoFisher) according to a company’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... Insoluble material was clarified for 10 min at 4°C at 16k rcf and incubated with 60 μl Dynabeads HIS-Tag isolation (10104D, Invitrogen) for 2 hour with rotation at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 0.01% Tween 20 and 50μM acetyl phosphate) containing 10 μl of pre-washed (3X) magnetic cobalt beads (Dynabeads His-Tag Isolation and Pulldown - Invitrogen) for a final volume of 200 μl in 96-well plates and incubated for 40 min at room temperature on a rotating wheel ...
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bioRxiv - Biophysics 2020Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The construct was transformed into bacterial DH10Bac component cells ...
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bioRxiv - Immunology 2021Quote: ... were coated at room temperature for 8 hours with 1 μg/mL 6x-His tag polyclonal antibody (PA1-983B, ThermoFisher), followed by overnight blocking with blocking buffer containing 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The PCR amplicon was digested with EcoR I and Age I and cloned to the same sites in pAc5.1/V5-His B (Thermo Scientific) to express the His-tagged protein ...
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bioRxiv - Microbiology 2020Quote: ... using the primers described in Table II followed by restriction digestion with BamHI and XbaI and ligation into pcDNA-V5/His (Invitrogen). The MV H and HPIV-1 HN were PCR amplified using the primers shown in Table II from genomic RNA of MV ...
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bioRxiv - Immunology 2021Quote: ... or in sterile Sorting Medium [RPMI 1640 supplemented with 10% (v/v) Heat-Inactivated Fetal Bovine Serum (HI-FCS; A3840001; Gibco)] ...
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bioRxiv - Genetics 2019Quote: ... were joined with the PCR amplified wild-type or activated Tkv cytoplasmic domain in the pIB/V5-His vector (ThermoFisher). The activated Tkv chimera (Tac-TkvA ...
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bioRxiv - Cell Biology 2022Quote: ... MEF cells were grown in high-glucose Dulbecco’s Modified Eagle Medium (DMEM; Cytiva) supplemented with 10% Heat-Inactivated Fetal Bovine Serum (HI FBS; Life Technologies), 10 mL per L of Antibiotic Antimycotic Solution (Penicillin/Streptomycin/Amphoterichin B ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently probed with alexa fluor 488-conjugated anti-His or anti-mouse IgG (H + L) Cross-Adsorbed Secondary Antibody Alexa Fluor 568 (ThermoFisher), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... with a N-terminal FLAG-tag and a C-terminal 6×His-tag preceded by a two-residues linker were subcloned into pFastBac 1 vector (Gibco). Sequence transposition into bacmid DNA using DH10Bac cells (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... and were maintained in DMEM high glucose (4500 mg/L) supplemented with 10% fetal bovine serum (FBS) (AlphaFBS-HI, Alphabioregen) and 1% Penicillin/Streptomycin antibiotics (Gibco), incubated at 37 °C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-HA(C29F4, Cell Signalling Technology,WB,1:1000, IF,1:500),anti-His (MA1-21315, Invitrogen; WB,1:10,000), anti-EEA1 antibody was a kind gift from Prof ...
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bioRxiv - Microbiology 2022Quote: ... Sequencing templates were prepared on the Ion Chef System using the Ion PI Hi-Q Chef Kit (Thermo Fisher Scientific). Sequencing was performed on an Ion Proton System using an Ion PI v3 chip (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... then eluted by incubation for 20 min in 11 µL Formamide-ROX350 mix (1000 µL Hi-Di Formamide (Thermo Fisher) and 8 µL ROX350 ladder (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... Expression vectors were made by cloning the indicated coding sequences into the pcDNA3.1/V5-His-TOPO vector (cat. no. K480001; Life Technologies). Constitutively active STAT5B (STAT5BCA ...
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bioRxiv - Developmental Biology 2022Quote: ... pMT-yki-HA was generated by cloning Yki-RG and HA tag into pMT-V5-His vector (Invitrogen, Cat# V412020). The Y281A ...
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bioRxiv - Synthetic Biology 2022Quote: Fluorescent proteins were purified in batch using His-tag affinity chromatography at room temperature according to the resin manufacturer’s instructions (Thermo Fisher). Lysates were supplemented with 10 mM imidazole and 600 μl was applied to HisPur Cobalt resin (300 μl ...
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bioRxiv - Cell Biology 2024Quote: All eukaryotic expression plasmids for pUL71 and pUL71 homologues were generated in the backbone of pEF1/Myc-His C expression vector (Invitrogen) by cloning using restriction enzymes ...
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bioRxiv - Immunology 2024Quote: Sera competition ELISA assay plates were coated with PBS containing 250 ng of anti-6x His tag monoclonal antibody (Invitrogen) and left covered overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... C2C12 cells transfected with EGFP-HRas WT/C181S/C184S in 35mm dishes were treated with 15d-PGJ2-Biotin (5 µM) in DMEM Hi Glucose medium (Gibco) supplemented with 1% Penicillin-streptomycin-Glutamine (Gibco ...
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bioRxiv - Biochemistry 2024Quote: Bacmids containing 6×His-MBP-TEV-nsp12 variants were generated using the Bac-to-Bac™ baculovirus expression system (Invitrogen). Sf9 cells were transfected with the bacmid DNA and baculovirus-containing cell supernatants were harvested 5 days after transfection ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were transferred from SDS-PAGE gel to the polyvinylidene difluoride membranes by western blotting and were detected using anti-His(C-term)-HRP antibodies (Invitrogen) as described previously (80).
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bioRxiv - Molecular Biology 2023Quote: ... Experiments were carried out using 8 μL of SETDB1-3TD (residues 196-403, N-terminal His tag) and 20X concentration Sy-pro Orange dye (5000X DMSO stock, ThermoFisher) and 2 uL of ligand for a final concentration of 20 μM of protein and 200 μM of ligand with 1.1% of DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... Sequences of full-length Dprs and DIPs with an N-terminal V5 tag were cloned into the pMT/BiP/V5-His A vector (Invitrogen) for expression in S2 Drosophila cells for PLC cleavage assay or into the pAcGP67 baculovirus transfer vector (BD Biosciences ...
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bioRxiv - Biochemistry 2022Quote: The region coding for the full-length Hermes transposase (Uniprot Q25438, with the mutations Q2E and K128G) was cloned into pBAD/Myc-His (Invitrogen) in such a way that the recombinant protein was not tagged ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 1% FBS (CPS Serum, Cat. No. FBS-500-HI) and 1X penicillin/streptomycin (Gibco, Cat. No. 15140-122), followed by centrifugation in an Eppendorf® centrifuge (model 5810 R ...
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bioRxiv - Immunology 2023Quote: ... Both cell lines were cultured in DMEM supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) and 100 U/mL penicillin-streptomycin (Gibco). Cells were incubated at 37°C with 5% CO2.
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Microbiology 2022Quote: ... Infected Vero cell monolayers were immediately overlaid with DMEM 2% Hi-FBS 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500). R ...
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bioRxiv - Developmental Biology 2023Quote: Peaks enriched in neural crest samples were cloned from genomic DNA using standard PCR methods using AccuPrime Hi-Fidelity Taq polymerase (Invitrogen). Primers used to amplify target enhancer sequences are provided in the Key Resources table (Table S1) ...
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bioRxiv - Biochemistry 2023Quote: ... A total of 2 mg of lysate per condition together with 25 μL of DynabeadsTM for His-Tag isolation (Invitrogen) were incubated with His6-AtLEGβ or the His6-proAtLEGβ-C211A dead mutant or lysate alone for an hour at room temperature with rotation ...