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Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for Recombinant Human ITGAV & ITGB5 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the protein extract was then measured with a BCA Protein Assay Kit (Thermo Fisher Scientific). A volume of each protein extract corresponding to 14 mg protein was transferred into a new 5-mL tube containing 150 µL streptavidin beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Protein extracts from cells were assessed for protein concentrations using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Equal amounts of protein lysates were added to SDS-PAGE gels and electrophoresis was performed ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the protein extract was determined using a BCA protein assay kit (CAT# 23225, Thermo Fisher Scientific) and adjusted to 500 ng ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the protein extract was determined using a BCA protein assay kit (CAT# 23225, Thermo Fisher Scientific) and adjusted to 2 μg/μL with the IP lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: Protein concentrations were determined using the BCA protein assay (Micro BCATM protein assay kit; Thermo Fisher Scientific, MA, USA) and CLARIOstar microplate reader (BMG labtech Durham ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentration was quantified using the “Protein Quantification BCA Assay” kit (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Biophysics 2021Quote: ... or 500 nM biotinylated oligonucleotides (Biomers) for external hybridization (H57-dSAv, H57-tSAv) and folding buffer (5 mM Tris (AM9855G, ThermoFisher Scientific), 50 mM NaCl (AM9759 ...
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bioRxiv - Genomics 2020Quote: ... The DNA overhangs were filled with biotinylated nucleotides by addition of 100 μL pre-mix (25 μL 0.4 mM Biotin-dATP (Thermo Fisher, 19524016), 25 μL 0.4 mM Biotin-dCTP (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... the selections were conducted using biotinylated α-cobratoxin that was captured using 80 µL of streptavidin-coated Dynabeads (Invitrogen, M-280). The concentration of α-cobratoxin was decreased through the three rounds of selections starting at 10 nM in the first round and ending at 20 pM in the third round ...
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bioRxiv - Molecular Biology 2021Quote: ... The scFvs were assessed for their binding to biotinylated α-cobratoxin (5 µg/mL) indirectly immobilized on black Maxisorp plates (Nunc) with streptavidin (10 µg/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... supernatants were incubated at 4°C with end-over-end rotation for 16-18 hrs with biotinylated Streptavidin T1 Dynabeads (ThermoFisher, 65601) previously conjugated with GFP antibodies (Sloan Kettering Institute Antibody Core ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed and then incubated for 2 hours at room temperature with biotinylated secondary antibodies (anti-rabbit 1:1000; Thermo Scientific) in the same buffer solution ...
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bioRxiv - Molecular Biology 2021Quote: ... The 4sU-labelled RNA was thiol-specific biotinylated by addition of 200 μg EZ-link HPDP-biotin (ThermoFisher Scientific, Cat# 21341), biotinylation buffer (10 mM Hepes-KOH pH 7.5 and 1 mM EDTA ...
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bioRxiv - Molecular Biology 2020Quote: ... Restriction fragments were biotinylated by supplementing the reaction with 60 μl of fill-in mix (0.25 mM biotin-14-dATP (Life Technologies, 19524016), 0.25 mM dCTP ...
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bioRxiv - Genomics 2020Quote: ... a mixture of 3 GFP-specific biotinylated primers (Supplementary Table 1; #120, #123 and #126) were used to immunoprecipitated GFP transcripts using Streptavidin C1 Dynabeads (Life Technologies). Following another round of DNase treatment ...
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bioRxiv - Genetics 2020Quote: ... Biotinylated ligation DNA was sheared to 250-500bp fragments followed by pull-down with MyOne Streptavidin T1 beads (Life technologies, 65602). Immobilized DNA fragments were end-repaired ...
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bioRxiv - Bioengineering 2020Quote: ... Biotinylated mPA was then modified with 10 equivalents of sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC; Thermo Fisher Scientific) at r.t ...
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bioRxiv - Synthetic Biology 2019Quote: ... Toehold strands were diluted to 1011 strands and mixed with biotinylated oligos at a ratio of 1:40 in a 50 µL reaction containing 2 mM MgCl2 (Invitrogen, Y02016) and 50 mM KCl (NEB ...
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bioRxiv - Neuroscience 2019Quote: ... Retrograde labeling of ascending and descending spinal cord neurons located in the spinal segment 15 was performed using dye injections with biotinylated dextran (3000 MW; ThermoFisher, D7135) into segments 10 or 20 respectively ...
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bioRxiv - Biophysics 2020Quote: ... Eluted protein fractions were site-specifically biotinylated using the BirA (Acro Biosystems Inc.) and subjected to a batch purification step with glutathione agarose (ThermoFisher Scientific) to remove the BirA ligase and gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2020Quote: Biotinylated heparin was prepared by mixing 2 mg of heparin and 2 mg of amine– PEG3–Biotin (Thermo Scientific, Waltham, MA) with 10 mg of sodium cyanoborohydride (NaCNBH3 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were subsequently washed in PBS-T three times and incubated in PBS-T containing 1:200 biotinylated goat anti-mouse IgG (Molecular Probes) as the secondary antibody at 25 °C for 1 day ...
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bioRxiv - Plant Biology 2021Quote: ... the purified protein was mixed with the appropriate concentration of each auxin before passing it over a streptavidin chip loaded with biotinylated SoIAA2 and SoIAA2Δ9 degron peptides (Thermo Fisher Scientific) (Supplementary Table 3).
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bioRxiv - Bioengineering 2021Quote: ... then incubated with 50 µL of 1 µM biotinylated peptide in peptide binding buffer (1% sheared salmon sperm DNA (Invitrogen, AM9680), 10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... B/Colorado/06/2017) were chosen as a historical antigen and were biotinylated using an EZ-Link Micro NHS-PEG4 Biotinylation Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... the captured viral biotinylated peptides were detected with 1 mg/ml pNPP (p-nitro-phenyl-phosphate, Thermo Fisher Scientific, cat: 34045) diluted in Diethanolamine substrate buffer 1X (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: The full-length CcdA and CcdA peptides of different lengths (Table S1) were biotinylated with EZ-linkTM NHS-Biotin (ThermoFisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... brain sections were washed 3 times with PBS-T and incubated with appropriate fluorophore-conjugated or streptavidine-fluorophore conjugated (for APOE biotinylated antibody) secondary antibodies (1:500, Life Technologies) together with nuclear stain Hoechst 33342 (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5 mg of these biotinylated lysates were adjusted to 500 μl and applied to 40 µl prewashed streptavidin-agarose beads (Thermo Scientific) and rotated overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Typhimurium was grown in BHI broth until OD600:1 and then biotinylated with EZ-link NHS-Biotin reagent (Thermo Fisher Scientific). After washing ...
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bioRxiv - Biophysics 2021Quote: ... The liposomes containing fluorophore-labeled hHv1 channels were retained on the PEGlyated surface coated with biotinylated Histag antibodies (1:200 dilution, ThermoFisher Inc.). Fluorophores were excited by 532 nm laser (∼1.0 W/cm2 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then incubated for 1 h at room temperature with secondary biotinylated antibodies or with the ABC kit (Cat. no. 32020, Thermo Fisher). SuperSignal West Femto (Thermo Scientific Cat n ...
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bioRxiv - Microbiology 2022Quote: 100 µl of purified and trypsin activated biotinylated rRV/VP4-BAP was incubated with 1 µl of streptavidin-Alexa Fluor 555 (2mg/ml) (ThermoFisher Scientific) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Poly-A selection was used to obtain only mRNA using poly-A biotinylated beads as per instructions (Dyna-beads Life technologies). The mRNA was used in an RT reaction that was specifically selected for the barcoded mRNA (Transcriptor High Fidelity Roche) ...
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bioRxiv - Cancer Biology 2022Quote: ... Half of the isolated total RNA underwent DNase treatment and a mixture of three GFP-specific biotinylated primers (#120, #123 and #126)(Supplementary Table 7a) were used to capture GFP transcripts with Streptavidin C1 Dynabeads (Life Technologies). An additional DNase treatment was performed ...
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bioRxiv - Bioengineering 2019Quote: ... the GVs were biotinylated by incubating them for 1 hour with a 10,000-fold molar excess of sulfo-NHS-biotin (Thermo Fisher Scientific). Then ...
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bioRxiv - Developmental Biology 2020Quote: ... supernatants were incubated at 4°C with end-over-end rotation for 16-18 hours with biotinylated Streptavidin T1 Dynabeads (ThermoFisher, 65601) previously conjugated with GFP antibodies (Sloan Kettering Institute Antibody Core ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated cRNA targets were prepared from 250 ng of total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, ThermoFisher Scientific). Labelled cRNA (750 ng ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated cRNA targets were prepared from 250 ng of total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, ThermoFisher Scientific). Labelled cRNA (750 ng ...
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bioRxiv - Neuroscience 2019Quote: ... courtesy of Peter Davies, Albert Einstein College of Medicine), biotinylated anti-phospho tauSer202/Thr205 AT8 (mouse, 1:500) (Thermo Scientific, USA) or anti-human tau CP27 (mouse ...
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bioRxiv - Molecular Biology 2021Quote: The biotinylated pre-mRNA (10 pmol) was immobilized with 5 µL of Dynabeads MyOne StreptavidinT1 magnetic beads (Invitrogen–Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: The biotinylated pre-mRNA (10 pmol) was immobilized with 5 µL of Dynabeads MyOne StreptavidinT1 magnetic beads (Invitrogen–Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... thoroughly rinsed twice with PBS and coated overnight at 4◦C with 100 µl/well of 1 mg/ml biotinylated BSA (Thermo Fisher) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... The cell surface was biotinylated by incubating cells for 10 min with 1 mg of EZ-Link-Sulfo-NHS-LC-biotin (Thermo Scientific) in Sorensen-Sorbitol buffer at pH 8 on ice ...
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bioRxiv - Immunology 2020Quote: ... thoroughly rinsed twice with PBS and coated overnight at 4°C with 100 μl/well of 1 mg/ml biotinylated BSA (Thermo Fisher) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... The cell surface was then biotinylated by incubating cells for 3 min with 30 mg of EZ-Link-Sulfo-NHS-LC-Biotin (Thermo Scientific) in Sorensen-Sorbitol buffer at pH 8 on ice ...
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bioRxiv - Cell Biology 2021Quote: ... The biotinylated DNA was pulled down by 10 μL of 10 mg/ml Dynabeads MyOne Streptavidin C1 beads (Life technologies, 65001). The beads were then re-suspended in 23 μL of 10 mM Tris-Cl pH 8.0 and libraries were prepared by on-bead reactions using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB ...
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bioRxiv - Immunology 2021Quote: ... B/Colorado/06/2017) were chosen as a historical antigen and were biotinylated using an EZ-Link Micro NHS-PEG4 Biotinylation Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Ferric Hb and ferrous Hb were individually biotinylated using sulfo-NHS-LC-Biotin (Sulfosuccinimidyl-6- {biotin-amido} hexanoate; Thermo Fisher Scientific) and immobilized on streptavidin beads in spin columns (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Secondary staining’s were performed for 1 h at room temperature in the dark with biotinylated goat anti-rabbit IgG AlexaFluor 405 (1:400; A-31556, Life Technologies) and goat anti-mouse IgG1 AlexaFluor 647 (1:800 ...