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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lot 134874), and 4-(2-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, purity ≥ 99.%, CAT #BP310-1, Lot 052975) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed once with PBS and a mixture of 1:1:2 (w/w) of pcDNA5-αSyn (4 μg): pOG44 Flp-recombinase expression vector (pOG44, Invitrogen #V600520):PEI in 2 mL of DMEM was added for 8 h ...
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bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed 1: 1 with SDS loading buffer and loaded on a NuPageTM 4-12% Bis-Tris PAGE Gel (Invitrogen™) along with Novex™ Sharp Pre-stained Protein Standards (range from 3.5 to 260 kDa ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Developmental Biology 2022Quote: ... embedded in paraffin using the Tissue-Tek VIP 6 (Sakura) tissue processor and of 6 µm sections obtained with a microtome (HM355S, Thermo Scientific). Z-shaped probes for Sema6d (565871) ...
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bioRxiv - Genomics 2021Quote: ... 600,000 cells were plated in 6 well plates and transfected with 2 μg plasmid DNA using 6 μl Lipofectamine LTX reagent (Thermo Fisher). Medium was exchanged after 16 h and harvested 24 h later ...
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bioRxiv - Cell Biology 2023Quote: ... The IL-6 levels in plasma were analyzed using a Mouse IL-6 Uncoated ELISA kit (#88-7064, Invitrogen, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... At 5-6 dpf each FoxP2.A:FingR(PSD95)+ larva was placed into individual wells of a 6-well plate (Thermo Fisher Scientific) containing approximately 10mL of fish water ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded at 30 000 cells/cm2 and were treated 6 h later with 6 µM of the calcium-sensitive Rhod2AM probe (ThermoFisher Scientific) for 1 h before refreshing the cell culture medium ...
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bioRxiv - Biochemistry 2024Quote: ... Transfection of 1 µg per well of plasmids encoding SARS-CoV-2 tag-free nucleocapsid protein was performed using 6 µL per well of Lipofectamine 2000 on HEK-293T cells with 70-80% confluency on 6-well plates (Invitrogen, 11668027). Compounds were added to the cells with fresh media after 6 hours of incubation with plasmids for 24-hour treatment before cell lysis and immunoblotting ...
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bioRxiv - Plant Biology 2024Quote: ... using primers GtEFF1 (5’-CCCTGCAAGCTCTTCCTCTTAG-3’) and GtEFR1 (5’-GCATGCGAGGTCCCAAAA-3’) with the TaqMan probe (5’-6FAM-ACTGCACAGACCATC-MGB-3’) (Thermo Scientific™, USA) (Keenan et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR targeting PTPRZ1 (5’-ACTCTGAGAAGCAGAGGAG-3’ and 5’-CTGTTGTCTGTAGTATCCATTAG-3’) or GAPDH (5’-TCAAGGCTGAGAACGGGAAG-3’ and 5’-CGCCCCACTTGATTTTGGAG-3’) was performed with Power SYBR™ Green PCR Master Mix (Applied Biosystems 4367659) in three technical replicates.
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bioRxiv - Cell Biology 2020Quote: ... MuSCs were stained for 3 hours with 1:500 donkey anti-rabbit Alexa 594 secondary antibody (Invitrogen) to detect cleaved caspase-3 ...
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bioRxiv - Genomics 2020Quote: ... and incubated for 1 hour in 50 µl DNAse reaction with 3 µl Turbo DNAse (ThermoFisher, AM2238) and 1X Turbo DNAse buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 3 % of homogenate was added to 1 mL of TRIzol Reagent (Fisher Scientific cat# 15-596-026) and RNA was isolated following the user guide referenced above ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were washed in PBS (3×10 min) and incubated with Cy5 streptavidin (1:500, Life Technologies) in PBS (1 h at room temperature) ...
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bioRxiv - Bioengineering 2020Quote: ... HUVECs were used between passage 1-3 Cells were enzymatically dissociated using TrypLE (ThermoFisher Scientific, MA, USA) for 5-7 minutes and centrifuged at 250G for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: The viability of the cells was examined by staining with 1 µM TOTO-3 iodide (Molecular Probes), which only stains dead cells (Zuliani et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse ENKD1 shRNA (nucleotides targeting 403-423 bp, 5′-CGCTCACCCAAGTATGACAAT-3′) were cloned into pLKO.1 (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... washed 3 times with PBS and the cellular nuclei stained with 1:1,000 of Hoechst (ThermoFisher, USA) for 15 min at RT.
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bioRxiv - Bioengineering 2021Quote: Membrane integrity was assessed after 1 and 3 days of culture using the Live/Dead assay (ThermoFisher). We used membrane integrity as a measure of cell viability in these studies ...
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bioRxiv - Neuroscience 2022Quote: BDA was visualized with fluorophore-conjugated streptavidin (Thermo Fisher Scientific, Table 2; 1:1,000 for 3 h). The reaction was enhanced using the biotinylated tyramine (BT)-glucose oxidase (GO ...
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bioRxiv - Bioengineering 2022Quote: ... Permethylated samples were combined in 1:3 ratio with 10 mg/mL 3,4-diaminobenzophenone (DABP, Acros Organics) matrix in 75% acetonitrile ...
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bioRxiv - Microbiology 2021Quote: ... media collected from infected cells and were mixed 1:3 (v/v) with TRIzol Reagent (ThermoFisher scientific) followed by chloroform extraction ...
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bioRxiv - Cancer Biology 2021Quote: ... The membrane was washed for 3 times and incubated with secondary antibodies (1:5000, Thermo Fisher Scientific) for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... NaCl 10 mM, MgCl2 3 mM, Tween-20 0,1%, Nonidet P40 Substitute 0.1%, Digitonin 0.01%, BSA 1%, Invitrogen™ RNAseout™ recombinant ribonuclease inhibitor 0.04 U/μL) ...
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bioRxiv - Immunology 2020Quote: ... 105 hCD3+ T cells were mixed with human T-Activator CD3/CD28 Dynabeads (Thermofisher, ratio 3:1) or PHA (1μg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... 3 × 105 EPCs were incubated with 1 µM LXW7-biotin in a binding buffer (1X HEPES (Gibco) containing 10% FBS (HyClone ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... Wells were washed 3 times and incubated with 1 μg/ml biotinylated anti-human IgE (A18803, Invitrogen) in 1% skim milk for 2 hours at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed 3 times with PBS and an aliquot of 1 ml of warmed TrypLE (Gibco) added to each well and incubated at 37°C for 10 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM EDTA) for 3 hours at room temperature using Slide-a-Lyzer mini dialysis cups (ThermoFisher), diluted to 100 μM ...
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bioRxiv - Immunology 2021Quote: ... with confluent bEnd.3 cell monolayers or pre-coated with collagen I (1 mg/ml, Life Technologies), and allowed to adhere for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were washed 3 × 10 min in TBS-T and secondary antibody incubation (1:750; Life Technologies) was performed for 1 hr ...
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bioRxiv - Microbiology 2022Quote: Anti-ORF8 mAb (#1-3-2) was coupled to aldehyde/sulfate latex beads (Thermo Fisher Scientific A37304). The antibody was mixed with the latex beads and shaken at room temperature overnight ...
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bioRxiv - Neuroscience 2022Quote: ... HLA-DR Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:50 Clone: LN-3, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 3 % of homogenate was added to 1 ml of TRIzol Reagent (Fisher Scientific cat# 15-596-026) and RNA was isolated as per the user guide referenced above ...
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bioRxiv - Neuroscience 2022Quote: ... biocytine filled neurons were revealed using Streptavidin Alexa Fluor 488 (1:1000; 3 h in dark; Invitrogen). Slices were then washed in PBS (1 h ...
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bioRxiv - Developmental Biology 2022Quote: ... 1μg total plasmid DNA (1:3 SAM to gRNA ratio) was diluted in Opti-MEM (Gibco #31985062) and mixed with p3000 reagent ...
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bioRxiv - Cell Biology 2022Quote: ... pDEST-AD-CYH2 (assay version 1) and pDEST-AD-AR68 (assay version 3) using Gateway Technology (Invitrogen). Attributes of these plasmids are summarized in Suppl ...
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bioRxiv - Molecular Biology 2024Quote: ... counted and total RNA was recovered from 3×106 cells using 1 ml of Trizol (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... 150ng of DNA was run on 3 lanes of a 1% eGel (Thermo Fisher Scientific, Waltham, MA) and size-selected into two populations ...
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bioRxiv - Bioengineering 2024Quote: ... for 1 hour before blocking with 3% (w/v) bovine serum albumin (BSA, Fisher Scientific, Cat # BP9703100). To detect ARP3 and GLUT4 ...
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bioRxiv - Molecular Biology 2024Quote: ... complexes (DNA to PEI MAX ratio, 1:3, w/w) formed in Opti-MEM (Thermo Fisher Scientific) via incubation for 15 min at 25°C ...
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bioRxiv - Genomics 2024Quote: ... cells were transfected with 1 µg of plasmid library using 3 µl Lipofectamine 3000 reagent (L3000008, Invitrogen, Carlsbad ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were incubated in Cyanine 3 (Cy3) goat anti-mouse (Thermo Fisher Scientific, A10521, 1:500) and Alexa Fluor 488 goat anti-rabbit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Samples were individually mixed with an equal volume of 1 µg/ml of 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate (DiI; Invitrogen), incubated for 15 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed with PBS 3 times and incubated with 1:10,000 DAPI (Invitrogen, Cat#D1306) for 5 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μg DNA and 3 μl transfection reagent were mixed in 100 μL Opti-MEM media (GIBCO) for 20min at room temperature before the mixture was added into each well ...
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bioRxiv - Genomics 2022Quote: ... The concentrate (about 300-450 µl) was mixed 1:3 with Trizol LS (ThermoFisher cat# 10296-010), and the RNA extracted using the DirectZol RNA kit (Zymo cat# R2052) ...