Labshake search
Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 prior to desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for LC-MS analysis.
-
bioRxiv - Biochemistry 2024Quote: ... Immunoprecipitated proteins were eluted three times, each time with 100 µL of elution buffer 2 (Pierce IgG Elution Buffer, pH ∼3 (Thermo Fisher Scientific); 1% (w/v ...
-
bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
-
bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
-
bioRxiv - Biochemistry 2024Quote: ... The clarified lysate (supernatant) was collected and mixed with 2-3 mL 50% slurry of Ni-NTA resin beads (ThermoFisher Scientific, #88221) washed twice in LB-MNA ...
-
bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
-
bioRxiv - Biochemistry 2024Quote: ... Samples (1µl) were loaded onto a C18 Acclaim PepMap100 trap-column (75 µm ID x 2 cm, 3 µm, 100Å, Thermo Fisher Scientific) for 3.5 minutes at 6 µL/min with 2% ACN ...
-
bioRxiv - Microbiology 2020Quote: ... 100μm lung slices were washed twice in 200 mM HEPES buffer then incubated for 20 min in a staining solution containing: 0.715 μM DAPI (4′,6-diamidino-2-phenylindole) (ThermoFisher Scientific D1306), 10 mg/L BODIPY 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene ...
-
bioRxiv - Cell Biology 2020Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
-
bioRxiv - Immunology 2022Quote: ... serum Igs were purified from H10ssF-immunized mice (combined 2-4 weeks after the third immunization) by using protein A/G (Pierce Thermo Scientific). Seven mg of purified heperimmune Igs or control Igs or 100 μg (5 mg kg-1 ...
-
bioRxiv - Neuroscience 2022Quote: ... Jurkats were treated with 150mJ of UV energy and incubated for 2-4 hours at 37°C in complete media consisting of RPMI 1640 Media (Gibco, 11875101), 10% FBS (R&D Systems ...
-
bioRxiv - Physiology 2022Quote: ... An aliquot of the polar phase was collected and vacuum-dried at 4°C and subsequently derivatized with 2% (w/v) methoxyamine hydrochloride (Thermo Scientific) in pyridine for 60 min following by 30 min sialyation N-tertbutyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were fixed for 15 min at 37°C in 4% paraformaldehyde and incubated with 2 μM Bodipy 493/503 (Bodipy 493, Molecular Probes) and 1 μg/ml of DAPI as published (9) ...
-
bioRxiv - Cell Biology 2022Quote: ... were added to 1-2 mg of lysates for overnight incubation at 4 °C before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
-
bioRxiv - Cell Biology 2022Quote: ... The remaining 50 μL were incubated at 4 °C for 2 h with 40 μg of HisPur Ni–NTA magnetic beads (Thermo Scientific) pre-equilibrated in binding buffer ...
-
bioRxiv - Immunology 2021Quote: ... and 30×103 cultured on the irradiated 40LB cells with IL-4 in supplemented RPMI-1640 media (final: 10% FBS, 1x GlutaMAX™, 50µM 2-mercaptoethanol Thermo Scientific™ ...
-
bioRxiv - Immunology 2021Quote: ... was incubated with 20 μg of NKp46-IgG1Fc or NKG2D-IgG1Fc fusion protein at 4° C with rotary agitation for 16 h and then incubated with 2 mM of DTSSP crosslinker (ThermoFisher Scientific) for 2 h on ice ...
-
bioRxiv - Bioengineering 2021Quote: ... Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol red-free DMEM + 10% FBS + 2 mM L-glutamine (Thermo Fisher). Cells were incubated with 100 nM TO-1 in HBSS starting 30 minutes prior to imaging and for the duration of the imaging period ...
-
bioRxiv - Bioengineering 2021Quote: ... and the cells were incubated for 50 minutes and DAPI (4′,6′-diamino-2-phenylindole) (Molecular Probes, Thermo Fisher Scientific, USA) for 5 minutes ...
-
bioRxiv - Bioengineering 2021Quote: ... and the cells were incubated for 50 minutes and DAPI (4′,6′-diamino-2-phenylindole) (Molecular Probes, Thermo Fisher Scientific, USA) for 5 minutes ...
-
bioRxiv - Bioengineering 2020Quote: ... the media was removed and the gels were washed once with PBS prior to the addition of 500uL of 1X PBS with Calcein AM (2 µM) and Ethidium Homodimer-1(4 µM) (Invitrogen, L3224). After 45 minutes of incubating the stains ...
-
bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
-
bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were stained for 30 min with 4’,6-diamidino-2-fenylindool (DAPI, 1:1000 in PBS, Thermo Fisher Scientific). After immunolabelling ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... slides were washed 3 x 5 min with PBS-TX and once with 0.1 M phosphate buffer pH 7.4 (0.08 M sodium phosphate dibasic and 0.02 M sodium phosphate monobasic) prior to coverslipping with ProLong Gold antifade reagent with 4’,6-diamidino-2-phenylindole [DAPI] (Invitrogen, P36931).
-
bioRxiv - Plant Biology 2021Quote: ... Tissues were counterstained with DAPI (4′,6-diamidino-2-phenylindole, 1 μg/ml in PBS) and mounted with ProLong Gold (ThermoFisher Scientific) using #1.5 coverslips (thickness ...
-
bioRxiv - Microbiology 2020Quote: ... cells were resuspended in 2 mL of 4 % FA in PBS (made from 16% Formaldehyde solution, Methanol-free from Thermo Scientific). Falcons were placed on a rotor for constant agitation for 45 min at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... Light organs were then counterstained overnight with a 1:750 dilution of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) in 5x SSC-Tween before mounting on slides with Vectashield (Vector Laboratories ...
-
bioRxiv - Neuroscience 2021Quote: Hair cell nuclei were specifically labeled by briefly exposing free-swimming larvae to 4’,6-Diamidino-2-Phenylindole (DAPI; Invitrogen/ThermoFisher) diluted to 2.5 μg/ml in E3 ...
-
bioRxiv - Genetics 2021Quote: ... cells were washed and suspended in flow cytometry buffer containing 1µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific).
-
bioRxiv - Molecular Biology 2020Quote: ... The transfection mixture was prepared by mixing 2 µg plasmid DNA and 4 µl Cellfectin-II reagent (Invitrogen, ThermoFisher Scientific, USA) to a final volume of 200 µl serum-free media ...
-
bioRxiv - Molecular Biology 2020Quote: ... once with 1X SSC at room temperature for 15 min and twice with 0.5X SSC at room temperature for 15 min and finally with 1X PBS buffer containing 0.5 µg/mL 4′,6-diamidino-2-phenylindole (DAPI) and mounted onto ProLong Gold antifade reagent (Thermo Fisher Scientific) mounting media.
-
bioRxiv - Molecular Biology 2020Quote: ... The transfection mixture was prepared by mixing 2 µg plasmid DNA and 4 µl Cellfectin-II reagent (Invitrogen, ThermoFisher Scientific, USA) to a final volume of 200 µl serum-free media ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were extracted using lysis buffer (8 M urea, 2 M thiourea, 4% CHAPS, 40mM DTT) supplemented with Halt protease inhibitor complete cocktail (Thermo Fisher) by bead beating (50 Hz for 3 five-minute cycles ...
-
bioRxiv - Cell Biology 2022Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
-
bioRxiv - Biochemistry 2022Quote: ... pH 7.4/50 mM KOAc/2 mM EDTA, pH 8/1 mM DTT/1 mM PMSF/1x Phosphatase inhibitor cocktail, Thermo Scientific), 0.3 g of glass beads (acid washed ...
-
bioRxiv - Physiology 2022Quote: ... Separation was performed with a Dionex Ionpac AS11-HC column (2 mm x 250 mm, 4 μm particle size, Thermo Fisher) at 30°C ...
-
bioRxiv - Physiology 2022Quote: ... The corpus was incubated in 50% betaisodona/PBS (Mundipharma GmbH) for 30 min at 4°C before being washed in different solutions for 2 min each: PBS (ThermoFisher Scientific), 0.1% octenidin in ddH2O (Serva Electrophoresis) ...
-
bioRxiv - Genomics 2022Quote: ... resuspended in ice cold PBS+BE (1 mg/mL BSA + 2 mM EDTA) and incubated for an hour at +4°C with Dynabeads Biotin Binder (Thermo Fisher). Cells were repeatedly washed with ice-cold sterile PBS while on magnet ...
-
bioRxiv - Microbiology 2022Quote: ... diluted 1:200 in blocking buffer for 30 min and mounted with ProLong Gold Antifade with DAPI (4′,6-diamidino-2- phenylindole; Thermo Fisher). Confocal laser micrographs were obtained with Zeiss LSM 880 laser microscope and analyzed with Zen black and Fiji software ...
-
bioRxiv - Biophysics 2022Quote: ... gels were functionalized with 0.5 mg/ml solution of sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Thermo Fisher Scientific) prepared in 50 mM HEPES buffer (Santa Cruz Biotechnologies) ...
-
bioRxiv - Plant Biology 2021Quote: ... two complementary oligonucleotides with 4-bp overhangs (Supplemental Table 2) were annealed and inserted by a Golden Gate reaction with BpiI (Thermo Scientific) and T4 DNA ligase (Thermo Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... cells were extensively washed and incubated with secondary antibodies in the presence of 4′,6-diamidino-2-phenylindole (DAPI, Molecular Probes) for 45 min at RT ...
-
bioRxiv - Cancer Biology 2020Quote: ... Pre-cleared chromatin (150 μg) was incubated with 4 μl (2 μg) of monoclonal mouse anti-V5 antibody (Invitrogen R960-25) for 1 hr at 4C with rocking ...
-
bioRxiv - Neuroscience 2020Quote: ... at 4 ng/μL and then pulled-down for 2 h using protein G-coupled dynabeads (50 μL, Thermo Fisher Scientific). After washing 3 times with 0.3 M NaCl solution ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were washed and mounted in media (0.5% N propyl gallate, 70% glycerol, 1M tris pH8.0) containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Cat. No. D1306).
-
bioRxiv - Plant Biology 2021Quote: ... The samples were washed for 8h at 4°C with gentle agitation with PBS (0.2% v/v Tween-20) replacing the buffer every 2 hours and incubated overnight at 4°C with the appropriate secondary antibodies (ThermoFisher Scientific), anti-Rabbit-Alexa 488 (RA3) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Captured cells were stained with 2 μM green-fluorescent calcein-AM and 4 μM red-fluorescent ethidium homodimer-1 (Molecular Probes) and visualized under an EVOS FL cell imaging station (Life Technologies ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) prior to 4’,6-diamidino-2-phenylindole (DAPI; 1:3000; Thermo Fisher) incubation for 5 minutes at room temperature to stain nuclei ...
-
bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...