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Citations for Thermo Fisher :
4201 - 4250 of 10000+ citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... D-PBS (D-PBS without Ca++ and Mg++) supplemented with 2% FBS and 3 mM cell culture grade EDTA (Life Technologies; Thermofisher) prior to monocyte isolation ...
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bioRxiv - Genomics 2021Quote: Human brain tissues were manually dissected into small 2-3 mm3 pieces and immersed in homogenization buffer (HBSS (Life Technologies, 14175-095), 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were loaded onto a trap column (Dionex-C18 trap column 75 μm × 2 cm, 3 μm, particle size, 100 Å pore size; ThermoFisher Scientific) at 5 μl/min before switching the pre-column in line with the analytical column (Dionex-C18 analytical column 75 μm × 50 cm ...
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bioRxiv - Biochemistry 2022Quote: ... The trap column used for the nano HPLC was a 2 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Thermo Fisher Scientific), and the separation column was a 12.5 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Nikkyo Technos ...
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bioRxiv - Cell Biology 2022Quote: ... a Thermo Scientific EASY-nLC 1000 liquid chromatography system coupled with an Acclaim PepMap 100 (2 cm x 75 μm, 3 μm particles, Thermo Fisher Scientific) pre-column in line with an EASY-Spray PepMap RSLC C18 reversed phase column (50 cm x 75 μm ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded at 3 μl/min for 6 min onto a 2 cm × 75 μm C18 trap column (Acclaim Pepmap 100, 3 μm, 300 Å, Thermo Scientific) in loading buffer (0.5% v/v formic acid ...
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bioRxiv - Microbiology 2022Quote: ... Roughly 1/3 of the plate (2-3 loopfuls) were added to 400 µl 1x Tris-Ethylenediaminetetraacetic acid (EDTA) pH 7.5 (Thermo Scientific, Waltham, USA) and heated at 80 ºC for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... For KV2.2 channels, NDS was substituted for normal goat serum (NGS, 3%) and Alexa Fluor® 555 goat anti-mouse IgG1 (Thermo Fisher Scientific Cat# A-21127 ...
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bioRxiv - Neuroscience 2022Quote: ... YFP-positive cells from 2-3 mice of each genotype were collected after viability screening with propidium iodide staining (ThermoFisher, Cat. # P3566) on a Beckman Coulter MoFlo platform in the Flow Cytometry and Cell Sorting Facility located in the Ottawa Hospital Sprott Center (Ottawa ...
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bioRxiv - Neuroscience 2022Quote: ... The peptides were trapped on an guard column (Acclaim PepMap 100, 75 µm x 2 cm, nano viper, C18, 3 µm, 100 Å, Thermo Fisher) using solvent A (0.1% Formic acid ...
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bioRxiv - Synthetic Biology 2024Quote: ... HEK293FT and HEK293FT-LP cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco 25300-054). All HEK293FT cell lines generated by engineering either the HEK293FT or HEK293FT-LP parent cell lines were cultured in the same way ...
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bioRxiv - Immunology 2024Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d. × 2 cm, Thermo Fisher Scientific) equilibrated with 0.05 % TFA in wather ...
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bioRxiv - Biochemistry 2024Quote: ... Injected sample fractions were preconcentrated and further desalted online using a PepMap C18 nano trap column (2 cm X 75 μm; particle size, 3 μm; pore size, 100 Å; Thermo Fisher) with a flow rate of 3.5 μL/min for 6 min ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d. x 2 cm, Thermo Fisher Scientific) equilibrated with 0.05% TFA in water ...
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bioRxiv - Cell Biology 2024Quote: ... water (5:3:2) and 3 µL was injected into a Ultimate 3000 UHPLC system coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific). Separation was done on an Accucore C18 column (150 x 2.1 mm ...
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bioRxiv - Neuroscience 2023Quote: Cell viability was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Cat. No. M6494, ThermoFisher Scientific, Waltham, MA, USA) to differentiate the range of toxic and nontoxic palmitic acid concentrations ...
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bioRxiv - Neuroscience 2023Quote: ... then washed 3X with PBS before mounting on 2” x 3” microscope slides with Prolong Glass hardset mounting media (Life Technologies, #P36984). Slides were allowed to dry overnight at room temperature before imaging.
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bioRxiv - Neuroscience 2023Quote: ... One microgram of tryptic peptides (in triplicate) were loaded on a C18 Acclaim PepMap100 trap-column (75 µm ID x 2 cm, 3 µm, 100Å, Thermo Fisher Scientific) for 3.5 minutes at 5 µL/minute with 2% ACN ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptide mixtures were injected in 0.1% TFA on a C18 Acclaim PepMap100 trap-column (75 µm ID x 2 cm, 3 µm, 100 Å, Thermo Fisher Scientific) for 3 min at 5 µL/min with 2% ACN ...
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bioRxiv - Immunology 2023Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 µm, 100 Å, 75 µm i.d. x 2 cm, Thermo Fisher Scientific) equilibrated with 0.05% trifluoroacetic acid in water ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Peptides were loaded first on a Trap column (Acclaim PepMap 100 3 µm C18 column of 2 cm length and 75 µm inner diameter, Thermo Fisher Scientific) and then injected on an analytical column (EasySpray 50 cm column 2 µm 100 Å C18 column with 75 µm inner diameter ...
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bioRxiv - Biochemistry 2023Quote: Sample digests were acidified with formic acid to a pH of 2-3 prior to desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for LC-MS analysis.
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bioRxiv - Cell Biology 2022Quote: ... The LC system was equipped with an Acclaim Pepmap 100 C18 HPLC nanoViper Trap Column (100 Å, 75 μm X 2 cm, 3 μm; ThermoFisher, USA) and an analytical column as above ...
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bioRxiv - Physiology 2023Quote: ... loaded onto a C18 RP nano-precolumn (Acclaim PepMap100, 75 μm internal diameter, 3 μm particle size and 2 cm length, Thermo Fisher Scientific), and separated on an analytical C18 nano-column (EASY-Spray column PepMap RSLC C18 ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides from the samples were automatically loaded into a trap column (Acclaim PepMap 100, 75 μm Å∼ 2 cm, C18, 3 μm, 100 A, Thermo Fisher) and eluted onto a 50 cm analytical column (PepMap RSLC C18 ...
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bioRxiv - Cell Biology 2023Quote: ... the sample was incubated for 3 s and blotted for 2 s using the freezing plunger Vitrobot I (Thermo Fisher Scientific, USA). Grids were quickly frozen in liquid ethane cooled by liquid nitrogen and stored in liquid nitrogen until checked ...
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Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchbioRxiv - Biochemistry 2023Quote: ... in water) and loaded on to the Acclaim PepMap 100 trap (2 cm long, 75 μm ID, C18 3 μm; Thermo Fisher Scientific). Analytical column (PICOCHIP ...
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bioRxiv - Cancer Biology 2023Quote: ... OCI-AML-2 and OCI-AML-3 cells were electroporated with sgRNA/Cas9 complex using the Neon transfection system (Thermo Fisher Scientific) under the condition of 1350 V ...
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bioRxiv - Cell Biology 2023Quote: siRNA transfection was performed with 25 nM siRNA duplexes (Integrated DNA Techonologies) shown in the table 2 and 3 uL of Lipofectamine RNAiMAX reagent (ThermoFisher Scientific, 13778150) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed 3×5 min in PBS and incubated for 2 h with an Alexa Fluor® Plus 555-conjugated secondary antibody (Invitrogen A32816) diluted in the blocking solution ...
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bioRxiv - Cancer Biology 2023Quote: ... was loaded onto a trap column (Acclaim1 PepMap 100 pre-column, 75 µm, 2 cm, C18, 3 mm, 100 Å, Thermo Scientific) and then separated on an analytical column (EASY-Spray column ...
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bioRxiv - Molecular Biology 2023Quote: ... The peptide lysates were initially trapped on a C18-reverse phase trapping column (Acclaim PepMapTM 100, 75 μm × 2 cm, particle size 3 μM, nanoViper, Thermo Fisher Scientific), and then separated on a C18-reverse phase analytical column (Double nanoViper™ PepMap™ Neo ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were loaded onto an Acclaim PepMap 100 trap column (75 μm × 2 cm nanoViper, C18, 3 μm 100 Å, ThermoFisher Scientific) at flow rate of 2 μL/min ...
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bioRxiv - Plant Biology 2023Quote: ... The peptides were applied to a C18 column (Acclaim® PepMap 100 pre-column, C18, 3 μm, 2 cm x 75 μm Nanoviper, Thermo Scientific) and subsequently separated using an analytical column (EASY-Spray column ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylation of Htz1(V126C) was carried out using N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)propionamide (HPDP-Biotin) (Thermo Fisher cat. # 21341), which has a pyridyl disulfide moiety ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... as separation column and an Acclaim PepMap 100 C18 column (75 µm inner diameter, 2 cm length, 3 µm particle size from Thermo Fisher Scientific) as trap column was used ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 µm, 100 Å, 75 µm i.d. x 2 cm, Thermo Fisher Scientific) equilibrated with 0.05% TFA in water ...
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bioRxiv - Molecular Biology 2024Quote: ... MEFs and HeLa) or 2 µL of Lipofectamine 2000 (Calu-3 and VeroE6) according to the manufacturer’s recommendation (ThermoFisher and Invitrogen, respectively). For co-transfections ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
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bioRxiv - Molecular Biology 2024Quote: ... peptides were concentrated using a PepMap100 C18 pre-column (75 µm x 2 cm, with 3 µm particle size; Thermo Fisher Scientific) and subsequently resolved on an EASY-Spray column (ES903 ...
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bioRxiv - Physiology 2024Quote: ... Samples were washed 3 times with 0.025% PBS-Tween20 and incubated with Alexa-conjugated secondary antibodies listed in Supplemental Table 2 (Life Technologies, 1/1000e) for 1 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were grown in 10 mL LB for 2-3 h and plasmids extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). From each library ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were loaded on a trapping column (2 cm x 75 µm ID. PepMap C18 3 µm particles, 100 Å pore size, Dionex, Thermo Scientific) and separated either on a 25 cm EASY-Spray column (25 cm x 75 µm ID ...
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bioRxiv - Genetics 2024Quote: TBB-2-associated siRNAs were isolated from mex5p::3xHA::tagRFP::TBB-2;GFP::NRDE-3(*NLS) embryo lysates and incubated with anti-HA magnetic beads (Thermo Scientific #88836). The beads were washed extensively and eluted with HA peptide (Thermo Scientific #26184).
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bioRxiv - Neuroscience 2024Quote: ... Retinas were incubated at 4°C for 3 days in PBS containing Alexa Fluor 488–conjugated isolectin GS-IB4 (Griffonia simplicifolia, 2 μg/ml, Invitrogen, Rockford, IL) and anti-laminin primary antibody (LAMA2 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were loaded onto a trapping column (Acclaim PepMap 0.075 mm, 2 cm, C18, 3 μm, 100 A; Thermo Fisher Scientific Corp.) in line with a nano-LC column (Acclaim PepMap RSLC 0.050 mm ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RT-qPCR was performed using a Quantstudio 3 Flex real-time PCR machine with 2× TaqMan Universal Master Mix II (Applied Biosystems, #2704844). Commercial primers/probe sets specific for mouse Gpr4 (Mm01322176_s1) ...
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bioRxiv - Molecular Biology 2024Quote: ... mCherry-LacI-FokI-WT and mCherry-LacI-FokI-D450A expressing vectors 24 were transfected in U2OS 2-6-3 cells using Lipofectamine LTX (Invitrogen; #15338-100) for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 µm, 100 Å, 75 µm i.d. x 2 cm, Thermo Fisher Scientific) equilibrated with 0.05% TFA in water ...
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bioRxiv - Immunology 2024Quote: ... 1% L-Glutamine and plated at a density of 2-3 × 106 cells per well in a round U-bottom Nunclon Delta treated 96-well plates with lids (Thermo Fisher Scientific). Plate was then centrifuged at 500 x g for 5 min at 4 °C before being stimulated with 100 μl of complete media + 1X eBioscience Cell Stimulation Cocktail (containing phorbol 12-myristate 13-acetate (PMA) ...